Abstract:
:The merozoite surface protein 2 of P. falciparum is highly polymorphic in nature, but has regions of almost complete conservation at its N- and C-termini. We produced a chimeric recombinant protein comprising these regions only (hereafter termed NC). Mice immunized with the NC antigen produce antibodies at levels comparable to those immunized with 1624, a full-length recombinant protein representing MSP2 from P. falciparum. Antisera raised against NC recognized P. falciparum schizonts by IFA and a P. falciparum protein of Mr 45 kDa by Western blot. However, antibody specificities were observed to differ between anti-NC and anti-1624 sera, and this resulted in differences in parasite recognition, despite similar levels of antibodies having been produced. The response to the NC antigen was also shown to be restricted in some mice (H2-d), but this was overcome by including appropriate T-cell help, which was accomplished by creating recombinant protein chimeras that contained NC and T-helper epitopes from Tetanus toxoid, or MSP119 from P. berghei.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Lawrence N,Stowers A,Mann V,Taylor D,Saul Adoi
10.1046/j.1365-3024.2000.00293.xkeywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
211-21issue
5eissn
0141-9838issn
1365-3024pii
pim293journal_volume
22pub_type
杂志文章abstract::The pathogenesis of Acanthamoeba keratitis (AK) is complicated. In our previous studies, TLR4 was found involved in the process of infection by Acanthamoeba in human corneal cells. The purpose of this study was to investigate the role of Toll-like receptor 4 (TLR4) signalling pathway in Wistar rats challenged with Aca...
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journal_title:Parasite immunology
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journal_title:Parasite immunology
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journal_title:Parasite immunology
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doi:10.1111/pim.12103
更新日期:2014-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2007-12-01 00:00:00
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journal_title:Parasite immunology
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更新日期:2004-11-01 00:00:00
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journal_title:Parasite immunology
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更新日期:2008-08-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1998-11-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1989-11-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2008.01066.x
更新日期:2009-01-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1991-05-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12416
更新日期:2017-02-01 00:00:00