Abstract:
:Antibody isotype and epitope specificities were examined in sheep immunized with EG95, a protective recombinant vaccine against hydatid disease. All sheep immunized with EG95 as a fusion protein with glutathione S-transferase (GST) produced prominent IgG antibodies against the EG95 portion of the protein. Linear, antibody-binding epitope specificities of EG95 were mapped using a series of 25 overlapping synthetic peptides. Three immunodominant regions were identified which generated specific IgG1 and IgG2 antibodies in the majority of vaccinated sheep. These regions corresponded to the EG95-derived sequences SLKAVNPSDPLVYKRQTAKF, DIETPRAGKKESTVMTSGSA and SALTSAIAGFVFSC. An additional immunogenic region was identified which induced almost exclusively IgG2 antibody. This epitope was located within the sequence TETPLRKHFNLTPV. The anti-parasitic, protective effects of the EG95 vaccine correlated with the detection of specific antibody to two or more of the four linear immunogenic regions. The identification of these immunogenic peptides of EG95 maybe useful in the development of a synthetic peptide vaccine as a derivative of the EG95 recombinant.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Woollard DJ,Gauci CG,Heath DD,Lightowlers MWdoi
10.1046/j.1365-3024.1998.00176.xsubject
Has Abstractpub_date
1998-11-01 00:00:00pages
535-40issue
11eissn
0141-9838issn
1365-3024journal_volume
20pub_type
杂志文章abstract::Carboxy-terminus of merozoite surface protein-1 (MSP-1(19) ) is the major protein on the surface of the plasmodial merozoite that acts as one of the most important blood-stage vaccine candidates. The present investigation was designed to evaluate the immune responses when either two recombinant antigens (rPvMSP-1(19) ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2011.01331.x
更新日期:2011-11-01 00:00:00
abstract::Human recombinant tumour necrosis factor (rTNF) enhanced monoclonal IgE-dependent eosinophil-mediated cytotoxicity to schistosomula of Schistosoma japonicum in a dose-dependent manner. The enhancing effect of rTNF was also observed for the antibody-dependent cytotoxicity of a human eosinophilic leukemia cell line, EoL...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1992.tb00033.x
更新日期:1992-11-01 00:00:00
abstract::Dipetalonema viteae (Filarioidea) infections were established in inbred strains of mice by the s.c. implantation of adult female worms and the resulting microfilaraemia and adult worm survival monitored. BALB/c mice were the most susceptible strain examined, showing a high level microfilaraemia of approximately 6 mont...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1985.tb00081.x
更新日期:1985-07-01 00:00:00
abstract::Net intestinal fluid movement was measured in immunized and non-immunized rats infected with the enteric stages of the nematode, Trichinella spiralis. Animals were studied 30 min, 5 days and 30 days after receiving infective larvae. Net water movement across the mucosal surface of the gut was measured in vivo by perfu...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1979.tb00711.x
更新日期:1979-01-01 00:00:00
abstract::In schistosomiasis endemic areas, intensities of reinfection after treatment are greater amongst young children than amongst adults, and high levels of parasite-specific IgE are associated with resistance to reinfection in an age-dependent manner. Previously we have reported that, in Western blots, a 22 kDa band was r...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1997.d01-186.x
更新日期:1997-02-01 00:00:00
abstract::In a double-blind study 393 seronegative dogs, residing in a holoendemic area for Leishmania donovani infantum infection, were randomly assigned to an immunization with a partly purified L.d. infantum-derived preparation, or received adjuvant only. During the first year of the study period the rate of infection was si...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1989.tb00676.x
更新日期:1989-07-01 00:00:00
abstract::Urine and stool specimens from 425 school children in Swaziland were examined for evidence of Schistosoma mansoni or Schistosoma haematobium infection. Concurrently, saliva collections were analysed for ABH secretory ability and blood samples were typed for ABO, Rh and Lewis groups. Among individuals infected with S. ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1979.tb00701.x
更新日期:1979-07-01 00:00:00
abstract::Trypanosoma congolense is one of the main species responsible for Animal African Trypanosomosis (AAT). As preventive vaccination strategies for AAT have been unsuccessful so far, investigating the mechanisms underlying vaccine failure has to be prioritized. In T. brucei and T. vivax infections, recent studies revealed...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12099
更新日期:2014-05-01 00:00:00
abstract::To determine the role of T cells and mast cells in intestinal pathology and immune expulsion of intestinal nematodes, worm burdens, goblet cell responses and villus structures were analysed in T- and B-cell-deficient severe combined immunodeficiency (SCID) mice, athymic nu/nu mice and mast cell deficient W/W(v) mice a...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2009.01123.x
更新日期:2009-08-01 00:00:00
abstract::The pathogenesis of Acanthamoeba keratitis (AK) is complicated. In our previous studies, TLR4 was found involved in the process of infection by Acanthamoeba in human corneal cells. The purpose of this study was to investigate the role of Toll-like receptor 4 (TLR4) signalling pathway in Wistar rats challenged with Aca...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2010.01247.x
更新日期:2011-01-01 00:00:00
abstract::The present paper reviews the available literature on the development of immunity to animal Schistosoma infections. The majority of the studies on animal schistosomiasis were performed in cattle and pigs and only Schistosoma mattheei, S. bovis and S. japonicum received particular attention, mainly because of their rec...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2005.00766.x
更新日期:2005-07-01 00:00:00
abstract::Chagas' disease or American trypanosomiasis due to Trypanosoma cruzi has existed at least since the time of the Inca empire and contributes significantly to cardiovascular morbidity and mortality in several countries of this continent. Due to the fundamental role of human class II molecules polymorphic residues in the...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
abstract::Mitogen-activated lymphocytes co-cultured with either purified Trypanosoma cruzi trypomastigotes or the filtrate of trypomastigote suspensions in culture medium manifest a significant decrease in their capacities to express p55 interleukin-2 receptor molecules (p55IL-2R) on their membrane and proliferate. In this stud...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb00889.x
更新日期:1995-04-01 00:00:00
abstract::This study examines several aspects of a polymorphic, immunodominant molecule (PIM) found in the protozoan parasite, Theileria parva. The antigen is present in all T.p. parva stocks examined, and in the related subspecies, T.p.bovis and T.p.lawrencei. It is the predominant antigen recognized by antisera from immune ca...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1991.tb00262.x
更新日期:1991-01-01 00:00:00
abstract::Naegleria fowleri, a free-living amoeba, is the causative pathogen of primary amoebic meningoencephalitis in humans and experimental mice. N. fowleri is capable of destroying tissues and host cells through lytic necrosis. However, the mechanism by which N. fowleri induces host cell death is unknown. Electron microscop...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2011.01297.x
更新日期:2011-07-01 00:00:00
abstract::Studies in animal models suggest that protection against malaria induced by intradermal (ID) administration of sporozoites is less effective compared to intravenous injection (IV). We investigated in a murine model the protective efficacy and immune responses after ID or IV immunization of sporozoites. Mice were immun...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12000.x
更新日期:2012-12-01 00:00:00
abstract::African trypanosomes are well known for their ability to avoid immune elimination by switching the immunodominant variant surface glycoprotein (VSG) coat during infection. However, antigenic variation is only one of several means by which trypanosomes manipulate the immune system of their hosts. In this article, the r...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2005.00791.x
更新日期:2005-10-01 00:00:00
abstract::Ascariasis is the most frequent soil transmitted helminthiasis and, as well as other helminth infections, is expected to influence the clinical presentation of allergic diseases such as asthma. Indeed, several clinical and experimental works have shown an important impact either increasing or suppressing symptoms, and...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12595
更新日期:2019-06-01 00:00:00
abstract::The overall performance of quantitative assays in the detection of anti-Toxoplasma IgG is satisfactory, but discrepancies between assays are not uncommon especially when IgG concentrations are close to the limit of detection of the tests. The purpose of our study was to identify soluble and membrane antigens extracted...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12139
更新日期:2014-12-01 00:00:00
abstract::The pathogenesis of the tissue lesions observed in American trypanosomiasis (Chagas' disease) seems to depend on a variety of mechanisms. The present study was dedicated to the investigation of the evolution of muscle lesions and its relationship with immunological and parasitological parameters. Mice were infected i....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1985.tb00091.x
更新日期:1985-09-01 00:00:00
abstract::Neospora caninum is a coccidian parasite of veterinary importance by causing abortion or stillbirth in cattle among other problems in diverse animal species. We assessed an experimental murine model for its suitability to study the immune response to N. caninum infection. Thus, wild-type (wt) C57BL/6 mice and B-cell (...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1999.00223.x
更新日期:1999-05-01 00:00:00
abstract::Leishmaniasis has recently garnered attention as one of the diseases 'most neglected' by drug research and development, as the current therapeutic modalities available for the patients are ridden with unacceptable toxicity due to high dosage of the drug, prolonged treatment schedules, resistance and prohibitive costs....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12071
更新日期:2014-01-01 00:00:00
abstract::Many studies on the role of merozoite surface protein 3 (MSP3) in immunity against malaria have focused on a conserved section of MSP3. New evidence suggests that polymorphic sequences within MSP3 are under immune selection. We report a detailed analysis of naturally-acquired antibodies to allele-specific and conserve...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2007.00951.x
更新日期:2007-08-01 00:00:00
abstract::African trypanosomes cause human and animal African trypanosomiases, which are chronic, debilitating and often fatal diseases of people and livestock in sub-Saharan Africa. The extracellular protozoan parasites are exemplars of antigenic variation. They direct host-protective B-cell and T-cell immune responses towards...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12387
更新日期:2016-12-01 00:00:00
abstract::A longitudinal study was undertaken in Burkina Faso among 293 children aged 6 months to 9 years in order to determine the correlation between an antibody response to several individual malarial antigens and malarial infection. It was found that the presence of a positive antibody response at the beginning of the rainy...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.0141-9838.2004.00705.x
更新日期:2004-06-01 00:00:00
abstract::There is good evidence for costs to both the uses of immune defences and their development and maintenance. The optimal defence will be a balance of these costs with the risk of infection and the virulence of the disease. It is therefore clear that the life-history characteristics of both host and parasite will impact...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12055
更新日期:2013-11-01 00:00:00
abstract::The chromadorea abundant larval transcript (ALT) family of proteins contains ALT one of the most studied putative vaccine candidate in experimental filariasis. This study reports the characterization of Wuchereria bancrofti 20/22 (Wb20/22) as a member of chromadorea, the ALT family of proteins from the L3 stage of W. ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12120
更新日期:2014-10-01 00:00:00
abstract::Despite the plethora of evidence on the consequences of host nutrition on their immune responses to gastrointestinal parasites, the identity of molecules and mechanisms that drive the manifestations of immunity under nutrient abundance are not yet known. This is partly due to limitations of the methodologies employed ...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2008.00993.x
更新日期:2008-02-01 00:00:00
abstract::Malaria parasites have a complex, multistage life cycle and there is a widely held view that each stage displays a distinct set of antigens presented to the immune system. Yet, molecular analysis of malaria parasites suggests that many putative antigenic targets are shared amongst the different stages. The specificiti...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12434
更新日期:2017-07-01 00:00:00
abstract::Previous work has shown that a protein extract enriched for cysteine protease activity (TSBP) prepared from adult Haemonchus contortus using thiol sepharose affinity chromatography confers substantial protection against a single challenge infection. TSBP comprised proteases and other proteins. Here, TSBP were further ...
journal_title:Parasite immunology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1365-3024.2005.00750.x
更新日期:2005-04-01 00:00:00