Emerging technologies and their applications in interactions between nutrition and immunity to gastrointestinal parasites in sheep.


:Despite the plethora of evidence on the consequences of host nutrition on their immune responses to gastrointestinal parasites, the identity of molecules and mechanisms that drive the manifestations of immunity under nutrient abundance are not yet known. This is partly due to limitations of the methodologies employed to date that have failed to give comprehensive answers. The great advancements in the technological front over the past few years have opened a window of opportunity to identify these effector molecules, to explore both the mechanisms and cellular pathways and to evaluate their importance in the immune response to parasites. The aim of this paper is to present some of the novel, high-throughput technologies that are currently available in immunology and to explore their use in advancing our knowledge in interactions between nutrition and immunity to nematode parasites, with special reference to sheep. In the first part, we introduce the technologies and we discuss advantages and pitfalls of their use. We bring in successful examples of how their employment advanced knowledge and improved our understanding of the mechanisms that regulate immune responses to pathogens (both micro- and macroparasites). In the second part, we focus on the impact of nutrition on the immune response to parasites, and explore how these technologies can be used to advance our knowledge of immunonutritional interactions. We use as our starting point well-established models that have been successfully used to investigate the consequences of nutrition on the manifestations of immunity to parasites, which we further consider in the context of the novel technologies. We conclude by emphasizing the great potential of the described methodologies in unravelling the complex interactions between nutrition and immunity, but we also recommend caution when interpreting the outcomes.


Parasite Immunol


Parasite immunology


Athanasiadou S,Huntley JF




Has Abstract


2008-02-01 00:00:00














  • Contribution of immunodiagnostic tests to epidemiological/intervention studies of cysticercosis/taeniosis in Mexico.

    abstract::Cysticercosis is acquired when swine ingest human faeces contaminated with Taenia solium eggs. Humans become tapeworm carriers when they ingest infected pork meat. They can also develop cysticercosis after inadvertently swallowing T. solium eggs. Human neurocysticercosis (NCC) is considered as a public health problem ...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Flisser A,Gyorkos TW

    更新日期:2007-12-01 00:00:00

  • Antilymphocyte autoantibody in lethal mouse malaria and its suppression by non-lethal malaria.

    abstract::Mice infected with lethal P. berghei or P. yoelii malaria develop anti-lymphocyte autoantibodies less than a week after infection. The autoantibodies are IgM, T-dependent and cytotoxic to a subpopulation of non-T lymphocytes at 37 degrees C in the presence of mouse or guinea-pig complement. Non-lethal P. yoelii and P....

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: de Souza JB,Playfair JH

    更新日期:1983-05-01 00:00:00

  • The role of macrophages in protective and pathological responses to Toxoplasma gondii.

    abstract::The ability of Toxoplasma gondii to cause clinical disease in immune-competent and immune-deficient hosts coupled with its ease of use in vitro and availability of murine models has led to its use as a model organism to study how the immune system controls an intracellular infection. This article reviews the studies t...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Park J,Hunter CA

    更新日期:2020-07-01 00:00:00

  • Teladorsagia circumcincta in the sheep abomasum: defining the role of dendritic cells in T cell regulation and protective immunity.

    abstract::Parasitic nematodes of the small-ruminant gastrointestinal tract pose a problem worldwide. The impact of these pathogens is worsened by the emergence of anthelmintic resistance to all three available classes of drugs. In addition to causing considerable economic loss, these parasites are detrimental to the health and ...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: McNeilly TN,Devaney E,Matthews JB

    更新日期:2009-07-01 00:00:00

  • An experimental model for canine visceral leishmaniasis.

    abstract::Seven mixed-breed dogs were challenged with either promastigotes or amastigotes of Leishmania donovani infantum strains recently isolated from naturally infected dogs. Different routes and numbers of parasites were utilized and each dog was monitored for at least 1 year post-infection. Anti-parasite specific antibody ...

    journal_title:Parasite immunology

    pub_type: 杂志文章


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    更新日期:1991-09-01 00:00:00

  • Mice immunized with a synthetic peptide construct corresponding to an epitope present on a Plasmodium falciparum antigen are protected against Plasmodium chabaudi challenge.

    abstract::Inhibitory monoclonal antibody (MoAb) 8E7/55 recognizes a parasitophorous vacuole membrane (PVM) antigen in Plasmodium falciparum. Previous studies have identified the epitope, DNNLVSGP, recognized by the MoAb. A synthetic peptide containing this sequence was synthesized and coupled to diphtheria toxoid (DT) and was f...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Lord R,Jones GL,Spencer L,Saul A

    更新日期:1993-11-01 00:00:00

  • Characterization of a polymorphic immunodominant molecule in sporozoites and schizonts of Theileria parva.

    abstract::This study examines several aspects of a polymorphic, immunodominant molecule (PIM) found in the protozoan parasite, Theileria parva. The antigen is present in all T.p. parva stocks examined, and in the related subspecies, T.p.bovis and T.p.lawrencei. It is the predominant antigen recognized by antisera from immune ca...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Toye PG,Goddeeris BM,Iams K,Musoke AJ,Morrison WI

    更新日期:1991-01-01 00:00:00

  • Modulation of IL-12 and IFNγ by probiotic supplementation promotes protection against Toxocara canis infection in mice.

    abstract::In this study, supplementation with the probiotic Saccharomyces boulardii promoted a reduction in intensity of infection by Toxocara canis and modulates cytokines mRNA expression in experimentally infected mice. IL-12 gene transcription had 40-fold increase in S. boulardii supplemented uninfected mice and sevenfold in...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: de Avila LF,de Leon PM,de Moura MQ,Berne ME,Scaini CJ,Leivas Leite FP

    更新日期:2016-05-01 00:00:00

  • Follow-up of Gambian children recruited to a pilot safety and immunogenicity study of the malaria vaccine SPf66.

    abstract::A pilot safety and immunogenicity trial of the malaria vaccine SPf66 was undertaken in The Gambia in 1993. One hundred and fifty infants aged 6-11 months were immunized with either 0.5 mg or 1.0 mg of SPf66 produced either in Colombia or in the USA or with a control vaccine. Children who received SPf66 experienced mor...

    journal_title:Parasite immunology

    pub_type: 临床试验,杂志文章


    authors: Bojang KA,Obaro SK,Leach A,D'Alessandro U,Bennett S,Metzger W,Ballou WR,Targett GA,Greenwood BM

    更新日期:1997-12-01 00:00:00

  • Gene expression profiling of ovine keratinocytes stimulated with Psoroptes ovis mite antigen--a preliminary study.

    abstract::Sheep scab is caused by the noninvasive mite, Psoroptes ovis, which initiates a profound pro-inflammatory skin response leading to lesion development. To investigate these early events between the skin and the parasite, primary ovine epidermal keratinocyte cultures were generated and challenged with mite derived antig...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Watkins CA,Mackellar A,Frew D,Mackie C,George A,Hopkins J,Burgess ST,McNeilly TN,Huntley JF

    更新日期:2009-06-01 00:00:00

  • Relationships between maternal malaria and malarial immune responses in mothers and neonates.

    abstract::Immune responses of 97 Gambian women and their neonates were studied. New methods distinguished between active and previous placental malaria, were used to examine relationships between maternal malaria and neonatal immune responses. Many placentas (61%) had active or previous malarial infection. Maternal and cord mal...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Rasheed FN,Bulmer JN,De Francisco A,Jawla MF,Jakobsen PH,Jepson A,Greenwood BM

    更新日期:1995-01-01 00:00:00

  • Effects of recombinant tumour necrosis factor on antibody-dependent eosinophil-mediated damage to Schistosoma japonicum larvae.

    abstract::Human recombinant tumour necrosis factor (rTNF) enhanced monoclonal IgE-dependent eosinophil-mediated cytotoxicity to schistosomula of Schistosoma japonicum in a dose-dependent manner. The enhancing effect of rTNF was also observed for the antibody-dependent cytotoxicity of a human eosinophilic leukemia cell line, EoL...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Janecharut T,Hata H,Takahashi H,Yoshida S,Saito H,Kojima S

    更新日期:1992-11-01 00:00:00

  • Experimental Schistosomiasis japonica in the pig: immunohistology of the hepatic egg granuloma.

    abstract::Use of the pig as an animal model in schistosomiasis research is increasing, but knowledge of the porcine immune response to schistosome infection is still very limited. We investigated the immunohistology of different maturation stages of the Schistosoma japonicum egg granuloma in pigs. Liver sections from pigs exper...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Hurst MH,Willingham AL,Lindberg R

    更新日期:2002-03-01 00:00:00

  • Biochemical characterization and localization of Fasciola hepatica 26-28 kDa diagnostic coproantigen.

    abstract::We have previously reported the usefulness of a 26-28 kDa coproantigen of Fasciola hepatica for diagnosis of infection. In this study, the 26-28 kDa coproantigen was biochemically characterized with the aid of monoclonal antibodies (MoAb) in an effort to better understand the biology of the antigen. Differential stain...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Abdel-Rahman S,O'Reilly KL,Malone JB

    更新日期:1999-06-01 00:00:00

  • Functional correlations between mucosal mast cell activity and immunity to Trichinella spiralis in high and low responder mice.

    abstract::Levels of intestinal mast cell protease (IMCP) were quantified in serum, gut tissue and in intestinal fluids taken from mice infected with Trichinella spiralis during primary and secondary infections. The ability to generate a mast cell response was dependent on the response phenotype of the mouse strain used. The mas...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Tuohy M,Lammas DA,Wakelin D,Huntley JF,Newlands GF,Miller HR

    更新日期:1990-11-01 00:00:00

  • The scavenger receptor MARCO is involved in Leishmania major infection by CBA/J macrophages.

    abstract::CBA/J mice are resistant to Leishmania major infection but are permissive to L. amazonensis infection. In addition, CBA/J macrophages control L. major but not L. amazonensis infection in vitro. Phagocytosis by macrophages is known to determine the outcome of Leishmania infection. Pattern recognition receptors (PRR) ad...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Gomes IN,Palma LC,Campos GO,Lima JG,DE Almeida TF,DE Menezes JP,Ferreira CA,Santos RR,Buck GA,Manque PA,Ozaki LS,Probst CM,DE Freitas LA,Krieger MA,Veras PS

    更新日期:2009-04-01 00:00:00

  • Identification of antigenic proteins from Echinostoma caproni (Trematoda) recognized by mouse immunoglobulins M, A and G using an immunoproteomic approach.

    abstract::Antigenic proteins of Echinostoma caproni (Trematoda) against mouse IgM, IgA, IgG, IgG1 and IgG2a were investigated by immunoproteomics. Excretory/secretory products (ESP) of E. caproni separated by two-dimensional (2D) gel electrophoresis were transferred to nitrocellulose membranes and probed with the different mous...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Sotillo J,Valero L,Sánchez Del Pino MM,Fried B,Esteban JG,Marcilla A,Toledo R

    更新日期:2008-05-01 00:00:00

  • Free radical generation and the course of primary infection with Nippostrongylus brasiliensis in congenitally athymic (nude) rats.

    abstract::The course of primary infections with Nippostrongylus brasiliensis was followed in nude (CBH/R nu/nu) and heterozygote (CBH/R nu/+) rats. In both nude and heterozygote rats peak egg production by N. brasiliensis occurred on days 7 and 8 post-infection. However, whereas in heterozygote rats egg production declined rapi...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Smith NC,Ovington KS,Bryant C

    更新日期:1991-11-01 00:00:00

  • Depleted intestinal goblet cells and severe pathological changes in SCID mice infected with Heligmosomoides polygyrus.

    abstract::To determine the role of T cells and mast cells in intestinal pathology and immune expulsion of intestinal nematodes, worm burdens, goblet cell responses and villus structures were analysed in T- and B-cell-deficient severe combined immunodeficiency (SCID) mice, athymic nu/nu mice and mast cell deficient W/W(v) mice a...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Hashimoto K,Uchikawa R,Tegoshi T,Takeda K,Yamada M,Arizono N

    更新日期:2009-08-01 00:00:00

  • Susceptible mice present higher macrophage activation than resistant mice during infections with myotropic strains of Trypanosoma cruzi.

    abstract::The kinetics of macrophage activation were compared among inbred strains of mice (C3H, BALB, B6 and B10.A) that are known to differ in their relative resistance to infections with the myotropic strains (Colombian and CL) of Trypanosoma cruzi. The parameters utilized to measure macrophage activation were rapid spreadin...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Russo M,Starobinas N,Ribeiro-Dos-Santos R,Minoprio P,Eisen H,Hontebeyrie-Joskowicz M

    更新日期:1989-07-01 00:00:00

  • Protection induced by vaccinating sheep with a thiol-binding extract of Haemonchus contortus membranes is associated with its protease components.

    abstract::Previous work has shown that a protein extract enriched for cysteine protease activity (TSBP) prepared from adult Haemonchus contortus using thiol sepharose affinity chromatography confers substantial protection against a single challenge infection. TSBP comprised proteases and other proteins. Here, TSBP were further ...

    journal_title:Parasite immunology

    pub_type: 临床试验,杂志文章


    authors: Knox DP,Smith SK,Redmond DL,Smith WD

    更新日期:2005-04-01 00:00:00

  • Dynamic two-photon imaging of the immune response to Toxoplasma gondii infection.

    abstract::Toxoplasma gondii is a highly successful parasite that can manipulate host immune responses to optimize its persistence and spread. As a result, a highly complex relationship exists between T. gondii and the immune system of the host. Advances in imaging techniques, and in particular, the application of two-photon mic...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Luu L,Coombes JL

    更新日期:2015-03-01 00:00:00

  • Germinal centre and marginal zone B cells expand quickly in a second Plasmodium chabaudi malaria infection producing mature plasma cells.

    abstract::Antibodies and B cells are critical in the protective immune response to the blood stage of the malaria parasite, Plasmodium chabaudi. However, little is known about the development of memory B cells and their differentiation into plasma cells during infection or after re-infection. Here we have shown that B cells wit...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Stephens R,Ndungu FM,Langhorne J

    更新日期:2009-01-01 00:00:00

  • Cellular responses and cytokine profiles in Ascaris lumbricoides and Trichuris trichiura infected patients.

    abstract::The impact of intestinal helminth infection, i.e. Ascaris lumbricoides and Trichuris trichiura, on cellular responsiveness and cytokine production was investigated in young adults. Ascaris-specific cellular responsiveness was higher in parasite-free endemic controls than in patients infected with T. trichiura, or A. l...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Geiger SM,Massara CL,Bethony J,Soboslay PT,Carvalho OS,Corrêa-Oliveira R

    更新日期:2002-11-01 00:00:00

  • Antibody-independent binding and activation of complement by Schistosoma mansoni adult worms.

    abstract::The in vitro binding serum complement (C) components to Schistosoma mansoni worms was studied. By exposing frozen sections of adult male and female parasites to normal human serum, binding of both classical and alternative pathway C components was observed. By immunofluorescence (IFL) microscopy selective binding of C...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Linder E,Huldt G

    更新日期:1983-03-01 00:00:00

  • A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.

    abstract::To study the role of autoreactive T cells in the pathogenesis of cardiomyopathy in Chagas' disease, we generated a cell line by repeated in vitro antigenic stimulation of purified splenic CD4+ T lymphocytes from a chronically Trypanosoma cruzi-infected mouse. Cells from this line were confirmed to be CD4+ CD8- and pro...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Ribeiro-Dos-Santos R,Mengel JO,Postol E,Soares RA,Ferreira-Fernandez E,Soares MB,Pontes-De-Carvalho LC

    更新日期:2001-02-01 00:00:00

  • Imaging trematode and nematode parasites.

    abstract::Recent advances in molecular genetics and in imaging mean that it is now increasingly feasible to image biological processes within helminth parasites and to visualize interactions between worms and their hosts. Moreover, other innovative imaging approaches that are not dependent on transgenic parasites have been appl...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Pearce EJ,Lok JB

    更新日期:2013-09-01 00:00:00

  • Reactive oxygen species-dependent necroptosis in Jurkat T cells induced by pathogenic free-living Naegleria fowleri.

    abstract::Naegleria fowleri, a free-living amoeba, is the causative pathogen of primary amoebic meningoencephalitis in humans and experimental mice. N. fowleri is capable of destroying tissues and host cells through lytic necrosis. However, the mechanism by which N. fowleri induces host cell death is unknown. Electron microscop...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Song KJ,Jang YS,Lee YA,Kim KA,Lee SK,Shin MH

    更新日期:2011-07-01 00:00:00

  • Evaluation of recombinant P23 protein as a vaccine for passive immunization of newborn calves against Cryptosporidium parvum.

    abstract::Cryptosporidiosis is a zoonotic protozoan disease that affects the gastrointestinal tract of animals and humans. Diarrhoea as the most important indication of the infection leads to high economic losses in livestock industries and is a life threatening infection in immunocompromised individuals. In the absence of the ...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Askari N,Shayan P,Mokhber-Dezfouli MR,Ebrahimzadeh E,Lotfollahzadeh S,Rostami A,Amininia N,Ragh MJ

    更新日期:2016-05-01 00:00:00

  • Schistosoma haematobium infection modulates Plasmodium falciparum parasite density and antimalarial antibody responses.

    abstract:AIMS:Schistosomiasis and malaria are endemic in sub-Saharan Africa where Schistosoma haematobium (Sh) and Plasmodium falciparum (Pf) coinfections are thus frequent. We explored the effect of Sh infection on antibody responses directed to Pf merozoite antigens and on malaria susceptibility in Beninese children. METHODS...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Tokplonou L,Nouatin O,Sonon P,M'po G,Glitho S,Agniwo P,Gonzalez-Ortiz D,Tchégninougbo T,Ayitchédji A,Favier B,Donadi EA,Milet J,Luty AJF,Massougbodji A,Garcia A,Ibikounlé M,Courtin D

    更新日期:2020-04-01 00:00:00