Abstract：
:Seven mixed-breed dogs were challenged with either promastigotes or amastigotes of Leishmania donovani infantum strains recently isolated from naturally infected dogs. Different routes and numbers of parasites were utilized and each dog was monitored for at least 1 year post-infection. Anti-parasite specific antibody levels were measured by enzyme-linked immunosorbence, immunofluorescence, crossed-immune electrophoresis and Western blotting on crude antigen. Western blotting on two pure parasite proteins, dp72 and gp70-2, was also done. Mitogenic and antigen-specific stimulation of peripheral blood lymphocytes was monitored; and the haematological, clinical and parasitological parameters measured. Dogs challenged with amastigotes exhibited a more pronounced humoral response to leishmanial antigens. Only in one case was strong antigen-specific proliferation detected. Clinical signs of disease, including hypergammaglobulinaemia, enlarged lymph nodes and the presence of parasites, were also more apparent in the dogs challenged with amastigotes. None of the seven dogs died. Serum antibodies to leishmanial antigens were apparent between 1.5 to 3 months following challenge and correlated with the appearance of enlarged lymph nodes, hypergammaglobulinaemia and the presence of parasites in tissue biopsies. Serum antibodies remained chronically high in these dogs throughout the period of the study. Only one dog (1/3) challenged intravenously with promastigotes and the dog challenged intradermally with amastigotes produced transient antibody responses to leishmanial antigen.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Abranches P,Santos-Gomes G,Rachamim N,Campino L,Schnur LF,Jaffe CLdoi
10.1111/j.1365-3024.1991.tb00550.xsubject
Has Abstractpub_date
1991-09-01 00:00:00pages
537-50issue
5eissn
0141-9838issn
1365-3024journal_volume
13pub_type
杂志文章abstract：:Nitric oxide (NO) plays diverse roles in a variety of pathological processes. We investigated the role of NO in Schistosoma japonicum egg-induced granuloma formation in a mouse hepatic model. Immunohistological analysis revealed that there is the most intense and extensive inducible nitric oxide (iNOS) expression 2 we...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3024.2001.00384.x
更新日期：2001-06-01 00:00:00
abstract：:Previous work has shown that a protein extract enriched for cysteine protease activity (TSBP) prepared from adult Haemonchus contortus using thiol sepharose affinity chromatography confers substantial protection against a single challenge infection. TSBP comprised proteases and other proteins. Here, TSBP were further ...
journal_title：Parasite immunology
pub_type： 临床试验,杂志文章
doi：10.1111/j.1365-3024.2005.00750.x
更新日期：2005-04-01 00:00:00
abstract：:Factors governing the sensitization or desensitization of basophils and mast cells are discussed. Mathematical models are proposed which illustrate the effects of rising or falling specific or non-specific IgE titres on the tendency of these cells to degranulate. The models presented are consistent with the hypothesis...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1981.tb00394.x
更新日期：1981-07-01 00:00:00
abstract：:In this study, supplementation with the probiotic Saccharomyces boulardii promoted a reduction in intensity of infection by Toxocara canis and modulates cytokines mRNA expression in experimentally infected mice. IL-12 gene transcription had 40-fold increase in S. boulardii supplemented uninfected mice and sevenfold in...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12314
更新日期：2016-05-01 00:00:00
abstract：:The zymosan-triggered chemiluminescence response of peritoneal exudate cells from mice infected with relatively virulent or avirulent Toxoplasma gondii strains was examined at various intervals after infection. Toxoplasma infections increased the background chemiluminescence compared with controls, but higher values w...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1984.tb00796.x
更新日期：1984-05-01 00:00:00
abstract：:On a B10 (Lshs) genetic background, the development of acquired T cell mediated immunity to Leishmania donovani infection in mice is under H-2 linked genetic control. Following intravenous inoculation of 10(7) amastigotes three phenotypic patterns of recovery have been described: 'early cure' (H-2r,s), 'cure' (H-2b) a...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1983.tb00760.x
更新日期：1983-09-01 00:00:00
abstract：:Dirofilaria immitis is the agent of the heartworm disease in canids and felids, and of pulmonary dirofilariosis in man. Like other filariae, D. immitis harbours endosymbion Wolbachia bacteriae. In this work we analyse the response of specific IgE antibodies against both D. immitis antigens and the Wolbachia surface pr...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2008.01060.x
更新日期：2008-11-01 00:00:00
abstract：:In vitro released products of the adult stage of the bovine lungworm. Dictyocaulus viviparus, were characterized according to their SDS-PAGE profile, glycosylation pattern, in vitro synthesis and antigenicity in the context of infection and vaccination with irradiated larvae. Biosynthetic labelling experiments with 35...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1993.tb00596.x
更新日期：1993-03-01 00:00:00
abstract：:Mice can be immunized to Plasmodium vinckei by repeated infections followed by cure. Such immunity is dependent on CD4 T cells and an architecturally modified spleen, but has little requirement for antibody. Thus, athymic mice can be exposed to P. vinckei and cured, but do not develop immunity. They are resistant to c...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1991.tb00548.x
更新日期：1991-09-01 00:00:00
abstract：:The caecal-dwelling nematode Trichuris muris provides a natural model of human whipworm infection. Resistance to T. muris is dependent on a host Th2 response, and CD4+Th2 cells migrate to the gut-associated lymphoid tissue (GALT) to elicit parasite expulsion. Thus, CD4+T cells infiltrate the caecal lamina propria duri...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2007.01017.x
更新日期：2008-03-01 00:00:00
abstract：:Helminth parasites induce a strong Th2 response, characterized by high levels of IgE and elevated signature cytokines such as IL-5. As many global deworming programmes are underway, there is concern that this might lead to emergence of Th1-mediated pathologies when the counterbalancing helminth-induced Th2 response is...
journal_title：Parasite immunology
pub_type： 杂志文章,随机对照试验
doi：10.1111/pim.12428
更新日期：2017-06-01 00:00:00
abstract：:The soluble portion of a large particle fraction which was derived from the muscle larva of T. spiralis was subjected to molecular sizing column chromatography using Sephacryl S-200. Five major peaks of 280 nm absorbing material were obtained. Analysis by immunoelectrophoresis revealed that each peak contained antigen...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1981.tb00405.x
更新日期：1981-10-01 00:00:00
abstract：:The knowledge on immune mechanisms of chronic urticaria (CU) at the cytokine level is widely scarce. We compared pro- and anti-inflammatory as well as Th1- and Th2-associated serum cytokine levels in two phenotypes of CU: associated with (CU+) and without (CU⁻) sensitization against Anisakis simplex, a ubiquitous fish...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2011.01282.x
更新日期：2011-06-01 00:00:00
abstract：:Previous studies have shown that Leishmania elongation initiation factor (LeIF) antigen causes a partial immunity against leishmaniasis. The antigen develops type I immunity by overexpression of inflammatory cytokines such as interleukin-12 (IL-12), IFN-γ and TNF-α. Therefore, We evaluated immune responses induced by ...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12310
更新日期：2016-04-01 00:00:00
abstract：:Opisthorchis viverrini infection causes opisthorchiasis and is a risk factor for cholangiocarcinoma via chronic inflammation. To investigate the mechanism of O. viverrini -induced liver disease, we applied a proteomic approach to examine alterations in hepatic protein levels in O. viverrini -infected hamsters. Two-dim...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2009.01189.x
更新日期：2010-05-01 00:00:00
abstract：:Low- and high-dose infections with the murine large intestinal nematode Trichuris muris are associated with induction of adaptive Th1 and Th2 responses, respectively, in mesenteric lymph nodes (MLN). Classical dendritic cells (cDC) accumulate in the large intestinal mucosa and MLN upon T. muris infection, yet their ro...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12458
更新日期：2017-10-01 00:00:00
abstract：:Antigenic proteins of Echinostoma caproni (Trematoda) against mouse IgM, IgA, IgG, IgG1 and IgG2a were investigated by immunoproteomics. Excretory/secretory products (ESP) of E. caproni separated by two-dimensional (2D) gel electrophoresis were transferred to nitrocellulose membranes and probed with the different mous...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2007.01019.x
更新日期：2008-05-01 00:00:00
abstract：:Studies in animal models suggest that protection against malaria induced by intradermal (ID) administration of sporozoites is less effective compared to intravenous injection (IV). We investigated in a murine model the protective efficacy and immune responses after ID or IV immunization of sporozoites. Mice were immun...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/pim.12000.x
更新日期：2012-12-01 00:00:00
abstract：:A comparison of Plasmodium chabaudi AS infection in BALB/c and BALB/c IgM-deficient mice demonstrated a protective role for IgM during infection. IgM-/- mice, unlike microMT mice, display competent B cell humoral immune responses. Increased susceptibility of IgM-/- mice was demonstrated by increased mortality, an adva...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.2005.00760.x
更新日期：2005-05-01 00:00:00
abstract：:In endemic areas, asymptomatic infection by the malaria parasite Plasmodium falciparum was found associated with elevated percentages of human host's mononuclear cell spontaneous in-vitro apoptosis. In Dielmo, a village where malaria is holoendemic, apoptosis was age-and parasite-dependent. In-vitro exposure of periph...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3024.2000.00307.x
更新日期：2000-06-01 00:00:00
abstract：:The effect of Toxocara (T.) canis antigen (TcAg) on lymphocytes was studied in vitro using normal murine spleen cells and human peripheral blood lymphocytes. TcAg prepared from adult worms stimulated murine spleen cells to proliferate at concentrations of 1-125 micrograms/ml. The responder cells TcAg are B cells, beca...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1995.tb01006.x
更新日期：1995-12-01 00:00:00
abstract：:Chagas' disease or American trypanosomiasis due to Trypanosoma cruzi has existed at least since the time of the Inca empire and contributes significantly to cardiovascular morbidity and mortality in several countries of this continent. Due to the fundamental role of human class II molecules polymorphic residues in the...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:Infections with the nematode Nematospiroides dubius fail to elicit mucosal mast cell (MMC) responses in the intestines of host mice, and suppress MMC responses generated by heterologous infection. Larval N. dubius have the capacity to prime for mastocytosis, and to elicit this response in primed mice during a challeng...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1988.tb00205.x
更新日期：1988-01-01 00:00:00
abstract：:The mechanisms by which antibodies interfere with Plasmodium growth are still under debate. Characterizing the asexual erythrocyte stages susceptible to antibodies from hyperimmune individuals is therefore a relevant contribution to vaccine research. In this study, using a virulent and synchronous murine malaria paras...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3024.2001.00419.x
更新日期：2001-11-01 00:00:00
abstract：:Antibody isotype and epitope specificities were examined in sheep immunized with EG95, a protective recombinant vaccine against hydatid disease. All sheep immunized with EG95 as a fusion protein with glutathione S-transferase (GST) produced prominent IgG antibodies against the EG95 portion of the protein. Linear, anti...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3024.1998.00176.x
更新日期：1998-11-01 00:00:00
abstract：:The carrier effect, using TNP-labelled schistosomula was used to measure the helper T-cell activity against the schistosomula surface in CBA mice exposed to 30 cercariae of Schistosoma mansoni. After infection the helper T-cell activity reached a peak in 8--10 days, but by 6 weeks it had declined to background levels....
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1981.tb00401.x
更新日期：1981-10-01 00:00:00
abstract：:The capacity of peritoneal exudate cells (PEC) obtained from mice infected or immunized with Plasmodium berghei to produce reactive oxygen species (ROS) and the biological basis of this response was investigated, using luminol-dependent zymosan-triggered chemiluminescence (CL). CL response of PEC from infected mice in...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1987.tb00509.x
更新日期：1987-05-01 00:00:00
abstract：:Malaria, particularly Plasmodium falciparum, continues to disproportionately affect pregnant women. In addition to the profoundly deleterious impact of maternal malaria on the health of the mother and foetus, malaria infection in pregnancy has been shown to affect the development of the foetal and infant immune system...
journal_title：Parasite immunology
pub_type： 杂志文章,评审
doi：10.1111/pim.12573
更新日期：2019-03-01 00:00:00
abstract：:Naturally acquired humoral immune responses to Pfs16, an integral membrane protein expressed in Plasmodium falciparum gametocytes and sporozoites, were investigated in The Gambia. A high prevalence of antibodies to this molecule was detected by peptide ELISA. Ninety-three per cent of the people taking part in a survey...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3024.1996.d01-17.x
更新日期：1996-10-01 00:00:00
abstract：:Inhibitory monoclonal antibody (MoAb) 8E7/55 recognizes a parasitophorous vacuole membrane (PVM) antigen in Plasmodium falciparum. Previous studies have identified the epitope, DNNLVSGP, recognized by the MoAb. A synthetic peptide containing this sequence was synthesized and coupled to diphtheria toxoid (DT) and was f...
journal_title：Parasite immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3024.1993.tb00574.x
更新日期：1993-11-01 00:00:00
