Prospect for an Entamoeba histolytica Gal-lectin-based vaccine.


:Entamoeba histolytica is the aetiological agent of invasive amoebiasis, the third leading parasitic cause of mortality in the world. The disease can be easily cured by chemotherapy; however, prevention, mainly in the form of vaccination, could greatly decrease the incidence of the disease, and possibly help in its eradication. The parasite's surface galactose and N-acetyl-d-galactosamine-inhibitable adherence lectin (Gal-lectin) is highly antigenic and is the most promising subunit vaccine candidate. We have generated a Gal-lectin-based DNA vaccine and tested its immunogenicity in mice. Although further optimization will probably be required, this vaccine could help in the generation of an amoebiasis DNA vaccine for use in humans.


Parasite Immunol


Parasite immunology


Gaucher D,Chadee K





Has Abstract


2003-02-01 00:00:00














  • Workshop on a detailed characterization of Trichinella spiralis antigens: a platform for future studies on antigens and antibodies to this parasite.

    abstract::In order to characterize immunodominant components of T. spiralis a workshop was organized. In this the reactivity of monoclonal and polyclonal antibodies, provided by different research groups, towards total extracts from adult, new born larvae and muscle larvae as well as to excretory/secretory components of muscle ...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Ortega-Pierres MG,Yepez-Mulia L,Homan W,Gamble HR,Lim PL,Takahashi Y,Wassom DI,Appleton JA

    更新日期:1996-06-01 00:00:00

  • Susceptibility of B-cell deficient C57BL/6 (microMT) mice to Neospora caninum infection.

    abstract::Neospora caninum is a coccidian parasite of veterinary importance by causing abortion or stillbirth in cattle among other problems in diverse animal species. We assessed an experimental murine model for its suitability to study the immune response to N. caninum infection. Thus, wild-type (wt) C57BL/6 mice and B-cell (...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Eperon S,Brönnimann K,Hemphill A,Gottstein B

    更新日期:1999-05-01 00:00:00

  • Immunoprophylactic potential of a 120 kDa Brugia malayi adult antigen fraction, BmA-2, in lymphatic filariasis.

    abstract::A 120 kDa antigen containing SDS-PAGE fraction BmA-2 isolated from Brugia malayi adult parasite was highly reactive with normal sera from filarial endemic area. BmA-2 was analysed for its prophylactic potential in in vitro and in vivo. Sera collected from BmA-2 immunized jirds induced a significant level (80 to 90%) o...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Chenthamarakshan V,Reddy MV,Harinath BC

    更新日期:1995-06-01 00:00:00

  • Genetic influence on the antibody response to antigens of Schistosoma mansoni in chronically infected mice.

    abstract::Immunoprecipitations of in vitro translation products of mRNA from adult Schistosoma mansoni worms with sera from chronically infected mice of 12 inbred strains reveal a strain-dependent heterogeneity in the antibody responses to schistosome antigens. Of the strains tested the response to the 86K antigen was restricte...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Kee KC,Taylor DW,Cordingley JS,Butterworth AE,Munro AJ

    更新日期:1986-11-01 00:00:00

  • Active suppression of interleukin 2 secretion in mice infected with Trypanosoma brucei AnTat 1.1.E.

    abstract::Impairment of T cell proliferation in mice infected with the pleomorphic Trypanosoma brucei AnTat 1.1 E clone was found not to be related to a depletion of T cells or to an absence of functional accessory cells, but rather to an active suppression of interleukin 2 (IL-2) production. Lymph node cells derived from infec...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Sileghem M,Hamers R,De Baetselier P

    更新日期:1986-11-01 00:00:00

  • The innate host defence against nematode parasites.

    abstract::Nematode parasites cause significant infections in both humans and animals. They are complex, multicellular organisms that present unique challenges for the host, in particular with respect to the recognition of their unusual surface structures by the innate defence system. The innate immune system is now recognized t...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: de Veer MJ,Kemp JM,Meeusen EN

    更新日期:2007-01-01 00:00:00

  • A DNA-prime/protein-boost vaccination regimen enhances Th2 immune responses but not protection following Schistosoma mansoni infection.

    abstract::DNA immunization represents a promising vaccine strategy that has been reasonably successful, and will likely play an even greater role in vaccine development as these vaccines continue to be improved. We have developed a partially protective DNA vaccine against schistosome infection based on a 23-kDa integral membran...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Da'Dara AA,Skelly PJ,Walker CM,Harn DA

    更新日期:2003-08-01 00:00:00

  • Functional correlations between mucosal mast cell activity and immunity to Trichinella spiralis in high and low responder mice.

    abstract::Levels of intestinal mast cell protease (IMCP) were quantified in serum, gut tissue and in intestinal fluids taken from mice infected with Trichinella spiralis during primary and secondary infections. The ability to generate a mast cell response was dependent on the response phenotype of the mouse strain used. The mas...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Tuohy M,Lammas DA,Wakelin D,Huntley JF,Newlands GF,Miller HR

    更新日期:1990-11-01 00:00:00

  • Calpain mediates degradation of cytoskeletal proteins during Jurkat T-cell death induced by Entamoeba histolytica.

    abstract::Entamoeba histolytica is known to induce host cell death via activation of calpain and caspases. In this study, we investigated the specific proteases involved in the degradation of cytoskeletal proteins during Jurkat T-cell death induced by E. histolytica. Amoebic trophozoites induced marked degradation of paxillin, ...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Lee YA,Kim KA,Shin MH

    更新日期:2011-06-01 00:00:00

  • Immunology of Taenia solium taeniasis and human cysticercosis.

    abstract::The life cycle of Taenia solium, the pork tapeworm, is continuously closed in many rural settings in developing countries when free roaming pigs ingest human stools containing T. solium eggs and develop cysticercosis, and humans ingest pork infected with cystic larvae and develop intestinal taeniasis, or may also acci...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Garcia HH,Rodriguez S,Friedland JS,Cysticercosis Working Group in Peru.

    更新日期:2014-08-01 00:00:00

  • Activation of complement in C-reactive protein positive sera by phosphorylcholine-bearing component isolated from parasite extract.

    abstract::Phosphorylcholine-bearing component levels in extracts of various parasites were determined by a capillary precipitin test using anti-phosphorylcholine Ig A myeloma protein. TEPC-15. Phosphorylcholine was demonstrated as a structural component not only in nematodes but also in trematodes and cestodes. The phosphorylch...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Sugane K,Oshima T

    更新日期:1983-07-01 00:00:00

  • Characterization of Neospora caninum iscom antigens using monoclonal antibodies.

    abstract::Neospora caninum is a cyst-forming coccidian parasite which in cattle can cause abortion and birth of feeble calves. For the serological diagnosis of neosporosis, antibodies directed to the parasite can be demonstrated by ELISA, utilizing N. caninum proteins incorporated into iscoms (immunostimulating complexes) as an...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Björkman C,Hemphill A

    更新日期:1998-02-01 00:00:00

  • Toxoplasma gondii-induced neuronal alterations.

    abstract::The zoonotic pathogen Toxoplasma gondii infects over 30% of the human population. The intracellular parasite can persist lifelong in the CNS within neurons modifying their function and structure, thus leading to specific behavioural changes of the host. In recent years, several in vitro studies and murine models have ...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Parlog A,Schlüter D,Dunay IR

    更新日期:2015-03-01 00:00:00

  • The scavenger receptor MARCO is involved in Leishmania major infection by CBA/J macrophages.

    abstract::CBA/J mice are resistant to Leishmania major infection but are permissive to L. amazonensis infection. In addition, CBA/J macrophages control L. major but not L. amazonensis infection in vitro. Phagocytosis by macrophages is known to determine the outcome of Leishmania infection. Pattern recognition receptors (PRR) ad...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Gomes IN,Palma LC,Campos GO,Lima JG,DE Almeida TF,DE Menezes JP,Ferreira CA,Santos RR,Buck GA,Manque PA,Ozaki LS,Probst CM,DE Freitas LA,Krieger MA,Veras PS

    更新日期:2009-04-01 00:00:00

  • Fasciola hepatica cathepsin L suppresses sheep lymphocyte proliferation in vitro and modulates surface CD4 expression on human and ovine T cells.

    abstract::Fasciola hepatica infection has been shown to suppress sheep lymphocyte proliferation in vitro and this is at least partially attributable to excretory/secretory products (ES) released by F. hepatica parasites. We identified a suppressive component in ES by analysing the effect of ES fractions, separated by gel filtra...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Prowse RK,Chaplin P,Robinson HC,Spithill TW

    更新日期:2002-02-01 00:00:00

  • Imaging trematode and nematode parasites.

    abstract::Recent advances in molecular genetics and in imaging mean that it is now increasingly feasible to image biological processes within helminth parasites and to visualize interactions between worms and their hosts. Moreover, other innovative imaging approaches that are not dependent on transgenic parasites have been appl...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Pearce EJ,Lok JB

    更新日期:2013-09-01 00:00:00

  • Malaria in pregnancy shapes the development of foetal and infant immunity.

    abstract::Malaria, particularly Plasmodium falciparum, continues to disproportionately affect pregnant women. In addition to the profoundly deleterious impact of maternal malaria on the health of the mother and foetus, malaria infection in pregnancy has been shown to affect the development of the foetal and infant immune system...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Harrington WE,Kakuru A,Jagannathan P

    更新日期:2019-03-01 00:00:00

  • Neutropenia augments experimentally induced Schistosoma japonicum egg granuloma formation in CBA mice, but not in C57BL/6 mice.

    abstract::The present study was designed to investigate the role of neutrophils during the development of Schistosoma japonicum egg granulomas, in C57BL/6 and CBA mice. Laid eggs were implanted into the liver and monoclonal antibody, RB6-8C5, was used to eliminate neutrophils. After daily antibody treatment between days 9 and 1...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Hirata M,Hara T,Kage M,Fukuma T,Sendo F

    更新日期:2002-09-01 00:00:00

  • The production of reactive oxygen species in mice infected or immunized with Plasmodium berghei.

    abstract::The capacity of peritoneal exudate cells (PEC) obtained from mice infected or immunized with Plasmodium berghei to produce reactive oxygen species (ROS) and the biological basis of this response was investigated, using luminol-dependent zymosan-triggered chemiluminescence (CL). CL response of PEC from infected mice in...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Li M,Li YJ

    更新日期:1987-05-01 00:00:00

  • Antibody-independent binding and activation of complement by Schistosoma mansoni adult worms.

    abstract::The in vitro binding serum complement (C) components to Schistosoma mansoni worms was studied. By exposing frozen sections of adult male and female parasites to normal human serum, binding of both classical and alternative pathway C components was observed. By immunofluorescence (IFL) microscopy selective binding of C...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Linder E,Huldt G

    更新日期:1983-03-01 00:00:00

  • Changes in microRNA expression in response to Schistosoma japonicum infection.

    abstract::Schistosomiasis japonicum is one of the most serious zoonotic diseases in the world. There is increasing evidence to show that host miRNAs are modulated following Schistosoma japonicum infection, and some of these miRNAs may play important regulatory roles in response to schistosome infection. Several host miRNAs have...

    journal_title:Parasite immunology

    pub_type: 杂志文章,评审


    authors: Hong Y,Fu Z,Cao X,Lin J

    更新日期:2017-02-01 00:00:00

  • Free radical generation and the course of primary infection with Nippostrongylus brasiliensis in congenitally athymic (nude) rats.

    abstract::The course of primary infections with Nippostrongylus brasiliensis was followed in nude (CBH/R nu/nu) and heterozygote (CBH/R nu/+) rats. In both nude and heterozygote rats peak egg production by N. brasiliensis occurred on days 7 and 8 post-infection. However, whereas in heterozygote rats egg production declined rapi...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Smith NC,Ovington KS,Bryant C

    更新日期:1991-11-01 00:00:00

  • Immunoregulation of genetically controlled acquired responses to Leishmania donovani infection in mice: the effects of parasite dose, cyclophosphamide and sublethal irradiation.

    abstract::On a B10 (Lshs) genetic background, the development of acquired T cell mediated immunity to Leishmania donovani infection in mice is under H-2 linked genetic control. Following intravenous inoculation of 10(7) amastigotes three phenotypic patterns of recovery have been described: 'early cure' (H-2r,s), 'cure' (H-2b) a...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Ulczak OM,Blackwell JM

    更新日期:1983-09-01 00:00:00

  • Distinct DC subsets regulate adaptive Th1 and 2 responses during Trichuris muris infection.

    abstract::Low- and high-dose infections with the murine large intestinal nematode Trichuris muris are associated with induction of adaptive Th1 and Th2 responses, respectively, in mesenteric lymph nodes (MLN). Classical dendritic cells (cDC) accumulate in the large intestinal mucosa and MLN upon T. muris infection, yet their ro...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Demiri M,Müller-Luda K,Agace WW,Svensson-Frej M

    更新日期:2017-10-01 00:00:00

  • Vaccination of goats against the trematode Schistosoma bovis with a recombinant homologous schistosome-derived glutathione S-transferase.

    abstract::We assayed the vaccine potentialities of a recombinant S. bovis-derived glutathione S-transferase (rSb28GST), member of a molecular family already shown to have protective capacities in the S. mansoni and S. japonicum models. Injection of the rSb28GST in Freund's Complete Adjuvant resulted in good specific IgG respons...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Boulanger D,Trottein F,Mauny F,Bremond P,Couret D,Pierce RJ,Kadri S,Godin C,Sellin E,Lecocq JP

    更新日期:1994-08-01 00:00:00

  • Restricted heterogeneity of T cell receptor variable alpha chain transcripts in hearts of Chagas' disease cardiomyopathy patients.

    abstract::The role of autoimmunity in the pathogenesis and progression of heart lesions in the chronic phase of Chagas' disease is controversial. In the absence of parasites in situ, the T cell infiltrate seen in heart lesions may be the primary determinant of tissue damage ultimately leading to heart failure and death. We used...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Cunha-Neto E,Moliterno R,Coelho V,Guilherme L,Bocchi E,Higuchi Mde L,Stolf N,Pileggi F,Steinman L,Kalil J

    更新日期:1994-04-01 00:00:00

  • Recombinant K28 antigen in ELISA in the diagnosis of canine visceral leishmaniosis.

    abstract::Crude total antigen (CTA) from Leishmania infantum and recombinant antigen K39 (rK39) and recombinant antigen K28 (rK28) were compared using an ELISA for the diagnosis of canine visceral leishmaniosis (CVL). Forty-two blood samples from healthy dogs from a nonendemic area and 80 blood samples from an endemic area for ...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Venturin GL,Bragato JP,Silva KL,de Lima VM

    更新日期:2015-12-01 00:00:00

  • Gene expression profiling of ovine keratinocytes stimulated with Psoroptes ovis mite antigen--a preliminary study.

    abstract::Sheep scab is caused by the noninvasive mite, Psoroptes ovis, which initiates a profound pro-inflammatory skin response leading to lesion development. To investigate these early events between the skin and the parasite, primary ovine epidermal keratinocyte cultures were generated and challenged with mite derived antig...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Watkins CA,Mackellar A,Frew D,Mackie C,George A,Hopkins J,Burgess ST,McNeilly TN,Huntley JF

    更新日期:2009-06-01 00:00:00

  • Immunization of mice with Plasmodium TCTP delays establishment of Plasmodium infection.

    abstract::Translationally controlled tumour protein (TCTP) may play an important role in the establishment or maintenance of parasitemia in a malarial infection. In this study, the potential of TCTP as a malaria vaccine was investigated in two trials. In the initial vaccine trial, Plasmodium falciparum TCTP (PfTCTP) was express...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Taylor KJ,Van TT,MacDonald SM,Meshnick SR,Fernley RT,Macreadie IG,Smooker PM

    更新日期:2015-01-01 00:00:00

  • Avian coccidiosis: changes in intestinal lymphocyte populations associated with the development of immunity to Eimeria maxima.

    abstract::The effect of infection and subsequent challenge with Eimeria maxima on the populations of lymphocytes in the small intestine of Light Sussex chickens was assessed by immunohistochemistry. T cells were characterized for CD3, CD4, CD8, TCR1 (gamma delta heterodimer) or TCR2 (alpha beta 1 heterodimer) markers, and B cel...

    journal_title:Parasite immunology

    pub_type: 杂志文章


    authors: Rothwell L,Gramzinski RA,Rose ME,Kaiser P

    更新日期:1995-10-01 00:00:00