Abstract:
:Gingival overgrowth is a common side-effect of the administration of cyclosporin A (CSA), phenytoin, and calcium blockers. To identify the signaling mechanisms possibly involved in the overgrowth, we examined how CSA affects the activities of MAP kinases and transcription factors in human gingival fibroblasts (HGF). The HGF were treated with CSA and TNF-alpha or PDGF. DNA-binding activity of NFAT, NFkappaB, and AP-1 transcription factors was determined by gel shift assay, and JNK, p38, and ERK1 and ERK2 activation was assessed by Western blot analysis of immunoprecipitates. The CSA inhibited NFAT, NFkappaB, and p38 and JNK activities; however, ERK1 and ERK2 were not affected significantly. AP-1 activity increased approximately 4.5-fold. Our results indicate that CSA affects signaling molecules in HGF differently from other cell types, and that a CSA-induced increase in AP-1 activity may affect the expression of fibrogenic molecules in gingiva and promote gingival overgrowth.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Bostrom A,Bharath H,Saulewicz A,Narayanan ASdoi
10.1177/154405910508400609keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
532-6issue
6eissn
0022-0345issn
1544-0591pii
84/6/532journal_volume
84pub_type
杂志文章abstract::Conservative techniques for improving the appearance of discolored teeth have become popular in the past decade. These include: in-office bleaching with 30% hydrogen peroxide, which is applied on etched enamel with a gauze pad and then exposed to a bleaching light; home bleaching with a mild form of peroxide, such as ...
journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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abstract::Blood cortisol levels of oral surgery patients were analyzed to objectively determine the effect of intravenous sedation on surgical stress. Results showed a measure of sedation but did not adequately reflect the stress of oral surgery or the changes produced by intravenous sedation. ...
journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:1975-09-01 00:00:00
abstract::1. A method has been developed and described for obtaining accurate thermal diffusivity values for dental cements and other restorative materials. This method employs specimens of a short cylindrical geometry which are readily fabricated from simply-constructed molds. 2. Results obtained for polycarboxylate and glass-...
journal_title:Journal of dental research
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journal_title:Journal of dental research
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abstract::A sintered hydroxyapatite (HAP) ceramic for use in wear studies was prepared from a commercial tricalcium phosphate. The sintered HAP had physical properties close to those of human enamel. The coefficient of friction and wear of the sintered HAP ceramic as characterized by tangential force, track width, and surface f...
journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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