Cyclosporin a affects signaling events differentially in human gingival fibroblasts.

Abstract:

:Gingival overgrowth is a common side-effect of the administration of cyclosporin A (CSA), phenytoin, and calcium blockers. To identify the signaling mechanisms possibly involved in the overgrowth, we examined how CSA affects the activities of MAP kinases and transcription factors in human gingival fibroblasts (HGF). The HGF were treated with CSA and TNF-alpha or PDGF. DNA-binding activity of NFAT, NFkappaB, and AP-1 transcription factors was determined by gel shift assay, and JNK, p38, and ERK1 and ERK2 activation was assessed by Western blot analysis of immunoprecipitates. The CSA inhibited NFAT, NFkappaB, and p38 and JNK activities; however, ERK1 and ERK2 were not affected significantly. AP-1 activity increased approximately 4.5-fold. Our results indicate that CSA affects signaling molecules in HGF differently from other cell types, and that a CSA-induced increase in AP-1 activity may affect the expression of fibrogenic molecules in gingiva and promote gingival overgrowth.

journal_name

J Dent Res

authors

Bostrom A,Bharath H,Saulewicz A,Narayanan AS

doi

10.1177/154405910508400609

keywords:

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

532-6

issue

6

eissn

0022-0345

issn

1544-0591

pii

84/6/532

journal_volume

84

pub_type

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