Abstract：
:The aim of our work was to investigate the presence of non-selective cation channels (NSCC) in freshly isolated smooth muscle cells from the human umbilical artery (HUA), one of the vessels involved in fetal-placental circulation. We studied the electrophysiological properties of NSCC using the patch-clamp technique in whole-cell configuration, and their possible role in the contractile state of intact vessels' rings. Recording with a high intracellular Cs(+) solution and a near physiological extracellular saline solution, we found a Gd(3+)-sensitive current (IC(50) = 1.05 microM) with a linear current-voltage relationship showing a reversal potential (E(rev)) of -2.1 +/- 1.2 mV (n =15 cells). La(3+) (100 microM) and Mg(2+) (5 mM) also blocked this current. In such conditions, inward currents were carried by Na(+) and Ca(2+); hence, a Na(+)-free solution inhibited only inward current (-67.3 +/- 11.4%, at -40 mV, n = 7, p < 0.05) and a Ca(2+)-Na(+)-free solution decreased the current even further with respect to values obtained in Na(+)-free solution (-69.8 +/- 8.8% at -40 mV, n = 9, p < 0.05). The permeability ratios (P(X)/P(Cs(+))) for monovalent and divalent cations were 1, 0.9, 0.7, 0.7, 0.7, and 0.5 where X = Cs(+), Na(+), Li(+), Ca(2+), Ba(2+) and Tris(+), respectively. In intact tissue, a 0 Ca(2+) extracellular solution, Gd(3+) (100-250 microM), La(3+) (200 microM) and Mg(2+) (5 mM) induced vasorelaxation in non-stimulated HUA rings.
journal_name
Placentajournal_title
Placentaauthors
Raingo J,Rebolledo A,Iveli F,Grassi de Gende AO,Milesi Vdoi
10.1016/j.placenta.2004.01.028keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
723-9issue
8-9eissn
0143-4004issn
1532-3102pii
S0143400404000426journal_volume
25pub_type
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