Abstract:
INTRODUCTION:To elucidate whether trophoblast expansion and invasion are modulated by androgen signaling in an in vitro co-culture model system with decidualizing endometrial stromal cells (ESCs). METHODS:We employed an in vitro co-culture model of early embryo implantation, consisting of human ESCs (EtsT499 cells) and spheroids generated by extravillous trophoblast (EVT) derived HTR8/Svneo. The ESCs were decidualized with 8-bromo-cAMP (8-br-cAMP) in the presence or absence of dihydrotestosterone (DHT) at various concentrations for 5 days before co-culture with EVT spheroids. Trophoblast expansion was monitored by fluorescent time-lapse imaging microscopy. ESCs motility was visualized by using CellTracker™ Orange CMRA fluorescent probe. Apoptosis of ESCs was detected by CellEvent™ Caspase-3/7® green detection reagent. Invasion assays were performed to quantify EVT invasion through a chemotaxis cell membrane. RESULTS:Expansion of EVT spheroids was significantly enhanced by decidualized compared to undifferentiated ESCs. This process was further stimulated if ESCs were first decidualized in the presence of DHT. In contrast to decidualized ESCs, undifferentiated cells actively migrated away from expanding EVT spheroids. Invasiveness of EVT toward decidualized ESCs was significantly attenuated in comparison to undifferentiated ESCs. DHT had no effect on EVT invasion. However, an inhibitor of intercellular gap junction communication significantly enhanced EVT invasion towards decidualized ESCs. CONCLUSIONS:These results indicate distinct roles for androgen signaling and gap junction formation in decidual cells in regulating trophoblast expansion and invasion.
journal_name
Placentajournal_title
Placentaauthors
Wongwananuruk T,Sato T,Kajihara T,Matsumoto S,Akita M,Tamura K,Brosens JJ,Ishihara Odoi
10.1016/j.placenta.2016.09.005subject
Has Abstractpub_date
2016-11-01 00:00:00pages
56-62eissn
0143-4004issn
1532-3102pii
S0143-4004(16)30510-0journal_volume
47pub_type
杂志文章相关文献
PLACENTA文献大全abstract:INTRODUCTION:Preterm birth is a heterogeneous phenotype, with placental abnormalities underlying many cases. The etiology of preterm births that occur in the absence of placental abnormalities, however, remain enigmatic and we considered that early pregnancy biomarkers may provide clues. METHODS:Women from a hospital-...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2019.12.007
更新日期:2020-01-01 00:00:00
abstract::Maternal and fetal cellular relationships in the normal human term placental basal plate were investigated by single and double immunohistochemical labelling techniques. Extravillous fetal trophoblast in the basal plate was uniformly reactive with markers of low-molecular-weight cytokeratins. The predominant maternal ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(88)90031-8
更新日期:1988-05-01 00:00:00
abstract:INTRODUCTION:Escherichia coli is recognized as an etiological bacteria associated with chorioamnionitis and the preterm premature rupture of fetal membranes. This pathological condition induces pro-inflammatory cytokines and degradative metalloproteinases, which are considered biological markers secreted in an acute st...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2014.12.011
更新日期:2015-03-01 00:00:00
abstract::Ovarian, endometrial and breast cancers are associated with several risk factors, such as low parity, infertility, early age at menarche, and late age at menopause. Frequently most of these risk factors coexist in infertile patients and some studies suggested that the different infertility causes can be involved in ca...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2008.08.007
更新日期:2008-10-01 00:00:00
abstract::The apoptosis cascade that plays a central role in normal and pathological processes is strictly controlled, in part by FLIP (Fas-associated death domain-like interleukin-1beta-converting enzyme-inhibitory protein), an inhibitor of caspase-8. Here, we report the expression of long and short isoforms of FLIP mRNAs and ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2005.08.004
更新日期:2006-06-01 00:00:00
abstract:OBJECTIVE:To evaluate pregnancy outcome and ultrasound diagnosis in patients with histologically-confirmed placental chorioangioma. STUDY DESIGN:Placentas with histological diagnosis of chorioangioma were identified and medical charts were reviewed for findings of ultrasound examinations. Pregnancy outcomes were compa...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2011.06.007
更新日期:2011-09-01 00:00:00
abstract::The gestational age and litter size in the guinea pig can be accurately assessed by taking an x-ray of the animal. From a measurement of the fetal head length on the x-ray plate a fetal weight can be calculated using previously accumulated data, included here. The gestational age can be assessed from this weight to wi...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(82)80022-2
更新日期:1982-01-01 00:00:00
abstract::Twinning and placental fusion occur at a low frequency in species like humans and mice. They are linked to reduced birth weight and intertwin growth discrepancy with important consequences for perinatal survival. Despite this, little is known about the histological characteristics of the twin or fused placenta and how...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2020.10.036
更新日期:2020-11-02 00:00:00
abstract:BACKGROUND:MicroRNAs (miRNAs) are small single-stranded RNA molecules working as post-transcriptional modulators of gene expression. Trophoblast cells are a heterogenous group of fetal cells forming the feto-maternal interface and displaying a wide spectrum of functions. The regulation of their behavior may partly unde...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2012.05.009
更新日期:2012-09-01 00:00:00
abstract::Cytophotometric measurement of the DNA content of Feulgen-stained nuclei in touch preparations of bovine placentomes (n =5) revealed that 8C nuclei occurred in all, 16C nuclei in two, and 32C nuclei in one specimen. The determination of ploidy level by in situ hybridization with a Y-chromosome specific DNA probe showe...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1053/plac.1999.0402
更新日期:1999-07-01 00:00:00
abstract::Physiological data indicate that both a transcellular and a paracellular pathway are available for transfer across the human placenta but the morphological correlate of the latter is uncertain. We measured the permeability of the dually perfused human placental cotyledon to the predominantly cationic protein horseradi...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(05)80249-8
更新日期:1993-01-01 00:00:00
abstract::Neutral endopeptidase 24.11 (NEP) is known to regulate cellular functions by degrading several bioactive peptides, such as gonadotropin-releasing hormone (GnRH). The present study was performed to clarify the mechanisms of NEP expression by GnRH in human choriocarcinoma (BeWo) cells. GnRH increased NEP expression and ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2003.09.002
更新日期:2004-02-01 00:00:00
abstract:BACKGROUND:Chronic maternal asthma is associated with reduced growth of the female fetus and normal growth of the male fetus. The mechanisms that control the differential effects of maternal asthma on the fetus have not been fully elucidated but alterations in placental function may play a role. In the current study we...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2011.05.005
更新日期:2011-08-01 00:00:00
abstract:INTRODUCTION:The most adverse perinatal outcome of intrahepatic cholestasis of pregnancy (ICP) is sudden fetal death related to acute fetoplacental hypoxia. Corticotrophin-releasing hormone (CRH), urocortin (UCN), and Wolfram syndrome 1 (WFS1) proteins may have a compensatory response to hypoxic stress. METHODS:A tota...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2019.06.378
更新日期:2019-08-01 00:00:00
abstract::Uptake and metabolism of adenosine by human placenta were studied using the single-circulation paired-tracer technique. When isolated cotyledons were perfused through the fetal (basal) circulation at mean pressures of 36 +/- 3.3 mmHg and mean flow rates of 6.6 +/- 0.3 ml/min the maximal [3H]adenosine uptake was 51.3 +...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/0143-4004(95)90030-6
更新日期:1995-10-01 00:00:00
abstract:OBJECTIVE:Receptor-activator of NF-κB ligand (TNFSF11, also known as RANKL) and its receptor RANK are essential regulators on bone remodeling, mammary gland development and hormone-associated breast cancer development. However, the expression pattern and role of RANKL/RANK axis in decidual stromal cells (DSCs) are uncl...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2013.04.020
更新日期:2013-08-01 00:00:00
abstract::The intrauterine environment has an important influence on placental development. In pre-eclampsia (PE) and intrauterine growth restriction (IUGR), early remodelling of spiral arteries has repercussions for uteroplacental blood flow. The IUGR placenta exhibits compromised growth of villous trees, a smaller intervillou...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2010.04.005
更新日期:2010-07-01 00:00:00
abstract:INTRODUCTION:Perfluorinated alkyl acids (PFAAs) are widely used in industry and consumer products. Pregnant women are exposed to PFAAs and their presence in umbilical cord blood represents fetal exposure. Interestingly, PFAAs are substrates for organic anion transporters (OAT) of which OAT4 is expressed in human placen...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2015.07.119
更新日期:2015-10-01 00:00:00
abstract::In the present study the possible role of decidual cells in the pregnancy-associated increased sensitivity of glomerular ecto-ADP-ase to endotoxin was investigated. Early (day 5) pregnant (E-Pr; n = 10), pseudopregnant (E-PSP; n = 10), (day 5), pseudopregnant rats with a decidualized uterus (E-DEC; n - 10), as well as...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(96)80013-0
更新日期:1996-03-01 00:00:00
abstract::Angiotensinogen is the substrate for the enzyme renin. In addition to the well-characterized low molecular weight forms of angiotensinogen, a high molecular weight form (HMrA) comprises about 15 per cent of the total angiotensinogen in plasma of pregnant women. In the current study the different forms of angiotensinog...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(05)80386-8
更新日期:1993-11-01 00:00:00
abstract::The dynamics of nickel (Ni) uptake, transfer, retention and clearance in fetuses and late gestational rats were investigated by assessing its distributions in placenta, maternal and fetal organs and tissues during the 24 h period after a single dose of (63)Ni intraperitoneal injection on gestational day 20. Peak (63)N...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2010.12.021
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:To evaluate umbilical cord abnormalities predisposing to mechanical cord compression and determine their relationship to adverse clinical outcomes and stasis-associated histologic changes in the placenta. METHODS:Placental slides of 224 singleton pregnancies with gross cord abnormality (true knots, long cord...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2009.09.005
更新日期:2009-12-01 00:00:00
abstract::Chorioallantoic placentae were obtained from a reproductively synchronized wild population of greater mustache bats for ultrastructural and immunocytochemical examination. The single discoidal placenta was always located in a lateral to mesometrial position on the right side of the uterus, which in the non-pregnant st...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(98)90079-0
更新日期:1998-07-01 00:00:00
abstract::A role for Substance P (SP) in human placenta is not known, although is possible that regulates placental physiology through the Neurokinin (NK)-1 receptor. Ten human normal placenta tissues were studied by immunohistochemistry to demonstrate the localization of NK-1 receptor and SP. An immunostaining pattern for NK-1...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2010.03.015
更新日期:2010-07-01 00:00:00
abstract::Amino acid transfer to the fetus is dependent on several different factors. While these factors can be understood in isolation, it is still not possible to predict the function of the system as a whole. In order to do this an integrated approach is required which incorporates the interactions between the different det...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2012.10.010
更新日期:2013-03-01 00:00:00
abstract::The aim of the present study was to evaluate the relationship between the retention of fetal membranes (RFM) and apoptosis of the cells in fetal membranes. The present study investigated mRNA and protein expressions of apoptosis-regulating factors: FAS, cellular FLICE-like inhibiting protein (cFLIP), BAX, BCL2, caspas...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2010.10.016
更新日期:2011-01-01 00:00:00
abstract::Superficial invasion of extravillous trophoblasts (EVTs) and impaired spiral artery remodelling are characterizing phenomena in pregnancies complicated by pre-eclampsia (PE) and fetal growth restriction (FGR). However, the underlying causes remain unclear. In this study, gene expression in decidua basalis tissue from ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/j.placenta.2010.04.003
更新日期:2010-07-01 00:00:00
abstract::Alpha-fetoprotein (AFP) is a major serum glycoprotein synthesized during fetal life mainly by the yolk sac and the fetal liver. At term, it reaches high concentrations in the maternal intervillous blood, which is in direct contact with the placental trophoblastic microvillous membrane, and this suggests the placental ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1053/plac.2002.0816
更新日期:2002-09-01 00:00:00
abstract::The ability of three streptococcal isolates of different pathogenicity to multiply in the tissues of pregnant mice was investigated following intravenous injection. The highly pathogenic isolate multiplied most rapidly whilst the isolate of low pathogenicity showed the least rapid growth within the observation period ...
journal_title:Placenta
pub_type: 杂志文章
doi:10.1016/s0143-4004(81)80001-x
更新日期:1981-07-01 00:00:00
abstract::Annexins are soluble proteins that bind to biological membranes containing negatively charged phospholipids, principally phosphatidylserine, in a Ca(2+)-dependent manner. Annexin-A5 (AnxA5), the smallest member of the annexin family, presents unique properties of membrane binding and self-assembly into ordered two-dim...
journal_title:Placenta
pub_type: 杂志文章,评审
doi:10.1016/j.placenta.2015.01.193
更新日期:2015-04-01 00:00:00