Expression of the mammalian homologue of vacuolar protein sorting 16 (Vps16p) in the mouse and rat brain.

Abstract:

:Synaptic vesicle fusion events are essential for synaptic transmission. Membrane docking and fusion events are highly regulated processes requiring the participation of a large number of soluble N-ethylmaleimide-sensitive factors (SNAREs) and SNARE-interacting proteins. We report the neuronal expression of mammalian homologue of vacuolar protein sorting 16 (mVps16p) which exhibits a high homology to the yeast Vps16p, a component of Class C Vps. Western blot and immunohistochemical analyzes revealed that mVps16p is highly expressed in the various brain areas and developmental stages tested. The immunoreactivities of mVps16p colocalized with microtubule associated protein 2, but not glial fibrillary acidic protein. In the primary culture of cortical neurons, mVps16p immunoreactivities were observed in the cell body and the neuronal processes, and highly enriched in the axonal outgrowths.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Kim BY,Ueda M,Nakamura Y,Kohsaka S,Akazawa C

doi

10.1016/j.neulet.2003.11.021

keywords:

subject

Has Abstract

pub_date

2004-01-30 00:00:00

pages

217-20

issue

3

eissn

0304-3940

issn

1872-7972

pii

S0304394003013351

journal_volume

355

pub_type

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