A nucleosome-free dG-dC-rich sequence element promotes constitutive transcription of the essential yeast RIO1 gene.

abstract：:Nitrosative stress results from an increase in reactive nitrogen species (RNS) within the cell. Though the RNS - nitric oxide (·NO) and peroxynitrite (ONOO-) - play pivotal physiological roles, at elevated concentrations, these moieties can be poisonous to both prokaryotic and eukaryotic cells alike due to their capac...

journal_title：Biological chemistry

authors： Tharmalingam S,Alhasawi A,Appanna VP,Lemire J,Appanna VD

更新日期：2017-10-26 00:00:00

abstract：:Cellular volume regulation is fundamental for numerous physiological processes. The volume-regulated anion channel, VRAC, plays a crucial role in regulatory volume decrease. This channel, which is ubiquitously expressed in vertebrates, has been vastly characterized by electrophysiological means. It opens upon cell swe...

journal_title：Biological chemistry

authors： Stauber T

更新日期：2015-09-01 00:00:00

abstract：:The expression of the cysteine protease cathepsin B is increased in early stages of human breast cancer.To assess the potential role of cathepsin B in premalignant progression of breast epithelial cells, we employed a 3D reconstituted basement membrane overlay culture model of MCF10A human breast epithelial cells and ...

journal_title：Biological chemistry

authors： Mullins SR,Sameni M,Blum G,Bogyo M,Sloane BF,Moin K

更新日期：2012-12-01 00:00:00

abstract：:The enzyme L-asparaginase is a crucial component in the treatment of acute lymphoblastic leukemia (ALL). As all asparaginases in clinical use are derived from microorganisms, immunological reactions are the most important adverse events associated with asparaginase treatment. Two different methods, phage display and t...

journal_title：Biological chemistry

authors： Werner A,Röhm KH,Müller HJ

更新日期：2005-06-01 00:00:00

abstract：:In order to enhance and target gene delivery we have previously established a novel method, termed magnetofection, which uses magnetic force acting on gene vectors that are associated with magnetic particles. Here we review the benefits, the mechanism and the potential of the method with regard to overcoming physical ...

journal_title：Biological chemistry

authors： Plank C,Schillinger U,Scherer F,Bergemann C,Rémy JS,Krötz F,Anton M,Lausier J,Rosenecker J

更新日期：2003-05-01 00:00:00

abstract：:The adipocytokine adiponectin and its receptor (AdipoR) comprise a new receptor-ligand system that is involved in a variety of clinically important morbidities such as obesity, type 2 diabetes and cardiovascular diseases. Adiponectin exerts a multitude of beneficial and tissue specific effects depending on its unique,...

journal_title：Biological chemistry

authors： Heiker JT,Kosel D,Beck-Sickinger AG

更新日期：2010-09-01 00:00:00

abstract：:Xeroderma pigmentosum complementation group D protein (XPD) is an iron-sulfur cluster containing 5'-3' helicase and, in humans, part of the transcription factor TFIIH. TFIIH is involved in nucleotide excision repair as well as in transcription initiation. Recently, three different groups have reported the structures o...

journal_title：Biological chemistry

authors： Wolski SC,Kuper J,Kisker C

更新日期：2010-07-01 00:00:00

abstract：:Metachromatic leukodystrophy is a lysosomal disorder caused by the deficiency of arylsulfatase A (ASA). This leads to the storage of the sphingolipid 3-O-sulfogalactosylceramide (sulfatide) in various cell types, such as renal tubular cells. Examination of mannose 6-phosphate receptor (MPR300)-dependent endocytosis re...

journal_title：Biological chemistry

authors： Klein D,Yaghootfam A,Matzner U,Koch B,Braulke T,Gieselmann V

更新日期：2009-01-01 00:00:00

abstract：:Dendritic cells (DCs) are an important link between innate and adaptive immunity. DCs get activated in inflamed tissues where oxygen tension is usually low, which requires the transcription factor hypoxia inducible factor (HIF)-1 for cellular adaptation. To investigate whether the HIF-1 transcriptional complex plays a...

journal_title：Biological chemistry

authors： Wobben R,Hüsecken Y,Lodewick C,Gibbert K,Fandrey J,Winning S

更新日期：2013-04-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) was used in monitoring human parvovirus B19 virus-like particle (VLP) antibody complexes from acute phase and past-immunity serum samples. The Oregon Green 488-labeled VLPs gave an average diffusion coefficient of 1.7 x 10(-7) cm2 s(-1) with an apparent hydrodynamic radius o...

journal_title：Biological chemistry

authors： Toivola J,Michel PO,Gilbert L,Lahtinen T,Marjomäki V,Hedman K,Vuento M,Oker-Blom C

更新日期：2004-01-01 00:00:00

abstract：:The vast majority of malignancies detected in renal parenchyma are diagnosed as renal cell carcinoma (RCC), whose subtype discrimination and determination of prognosis may contribute to the selection of the adequate therapy. Recently, a new class of small non-coding RNAs, known as microRNAs, has proven to be among the...

journal_title：Biological chemistry

authors： Papadopoulos EI,Petraki C,Gregorakis A,Fragoulis EG,Scorilas A

更新日期：2016-06-01 00:00:00

abstract：:Historically considered as accessory cells to neurons, there is an increasing interest in the role of astrocytes in normal and pathological conditions. Astrocytes are involved in neurotransmitter recycling, antioxidant supply, ion buffering and neuroinflammation, i.e. a lot of the same pathways that go astray in Alzhe...

journal_title：Biological chemistry

authors： Zulfiqar S,Garg P,Nieweg K

更新日期：2019-08-27 00:00:00

abstract：:The accumulation of cholesterol is a general feature of cancer tissue, and recent evidence suggests that cholesterol plays critical roles in the progression of cancers, including breast, prostate, and colorectal cancers. The dysregulation of metabolic pathways, including those involved in cholesterol biosynthesis, is ...

journal_title：Biological chemistry

authors： Murai T

更新日期：2015-01-01 00:00:00

abstract：:Synthetic biologists aim at engineering controllable biological parts such as DNA, RNA and proteins in order to steer biological activities using external inputs. Proteins can be controlled in several ways, for instance by regulating the expression of their encoding genes with small molecules or light. However, post-t...

journal_title：Biological chemistry

authors： Di Ventura B,Mootz HD

更新日期：2019-03-26 00:00:00

abstract：:The transcription factor GLI1 is one of three vertebrate members of the GLI family, which is characterized by a highly conserved DNA-binding domain of five zinc fingers. We have analyzed whether human GLI1 is a target of PKA regulation. It was found that PKA inhibits GLI1 transcriptional activity. However, no evidence...

journal_title：Biological chemistry

authors： Kaesler S,Lüscher B,Rüther U

更新日期：2000-07-01 00:00:00

abstract：:Human kallikrein-related peptidases (KLKs), including the well-known prostate cancer biomarker, prostate-specific antigen (PSA-KLK3), along with 14 other serine proteinase KLK family members are now known to regulate cells by cleaving and activating members of the G-protein-coupled proteinase-activated receptor (PAR) ...

journal_title：Biological chemistry

authors： Hollenberg MD

更新日期：2014-09-01 00:00:00

abstract：:Abstract Fanconi anemia (FA) is a rare cancer-prone genetic disorder characterized by progressive bone marrow failure, chromosomal instability and redox abnormalities. There is much biochemical and genetic data, which strongly suggest that FA cells experience increased oxidative stress. The present study was designed ...

journal_title：Biological chemistry

authors： Petrovic S,Leskovac A,Kotur-Stevuljevic J,Joksic J,Guc-Scekic M,Vujic D,Joksic G

更新日期：2011-07-01 00:00:00

abstract：:In the SalI system, endonuclease activity can be only achieved in the presence of a functional modification gene. Thus, the DNA methyltransferase is involved in the control of restriction. By fusion of the restriction gene of the SalI system to the modification gene of the isospecific HgiDII system a hybrid type II re...

journal_title：Biological chemistry

authors： Pélaez AI,Ribas-Aparicio RM,Gómez A,Rodicio MR

更新日期：1998-04-01 00:00:00

abstract：:Insulin contains three disulfide bonds, one intrachain bond, A6-A11, and two interchain bonds, A7-B7 and A20-B19. Site-directed mutagenesis results (the two cysteine residues of disulfide A7-B7 were replaced by serine) showed that disulfide A7-B7 is crucial to both the structure and activity of insulin. However, chemi...

journal_title：Biological chemistry

authors： Guo ZY,Jia XY,Feng YM

更新日期：2004-12-01 00:00:00

abstract：:The relationship between the structure and activity of meta- and para-hydroxylated monophenols was studied during their tyrosinase-catalysed hydroxylation and the rate-limiting steps of the reaction mechanism were identified. The para-hydroxylated substrates permit us to study the effect of a substituent (R) in the ca...

journal_title：Biological chemistry

authors： Fenoll LG,Rodríguez-López JN,Varón R,García-Ruiz PA,García-Cánovas F,Tudela J

更新日期：2000-04-01 00:00:00

abstract：:The physiological roles of the intracellular iron and redox regulatory systems are intimately linked. Iron is an essential trace element for most organisms, yet elevated cellular iron levels are a potent generator and amplifier of reactive oxygen species and redox stress. Proteins binding iron or iron-sulfur (Fe/S) cl...

journal_title：Biological chemistry

authors： Mühlenhoff U,Braymer JJ,Christ S,Rietzschel N,Uzarska MA,Weiler BD,Lill R

更新日期：2020-11-26 00:00:00

abstract：:Dipeptidyl peptidase-IV has been demonstrated to play a role in cancer biology by many authors. Since then, additional proteins possessing similar enzymatic activity have been described and their role in cancerogenesis has been hypothesized. To assess the complexity of these 'Dipeptidyl peptidase-IV activity and/or st...

journal_title：Biological chemistry

authors： Sedo A,Busek P,Scholzová E,Malík R,Vlasicová K,Janácková S,Mares V

更新日期：2004-06-01 00:00:00

abstract：:Kallikrein-related peptidases (KLKs) are a family of serine proteases that were shown to be useful cancer biomarkers. KLKs have been shown to be dysregulated in prostate cancer (PCa). microRNAs (miRNAs) are short RNA nucleotides that negatively regulate gene expression and have been reportedly dysregulated in PCa. We ...

journal_title：Biological chemistry

authors： White NM,Youssef YM,Fendler A,Stephan C,Jung K,Yousef GM

更新日期：2012-04-01 00:00:00

abstract：:The ubiquitous calpains, mu- and m-calpain, have been implicated in essential physiological processes and various pathologies. Cell-permeable specific inhibitors are important tools to elucidate the roles of calpains in cultivated cells and animal models. The synthetic N-acetylated 27-mer peptide derived from exon B o...

journal_title：Biological chemistry

authors： Gil-Parrado S,Assfalg-Machleidt I,Fiorino F,Deluca D,Pfeiler D,Schaschke N,Moroder L,Machleidt W

更新日期：2003-03-01 00:00:00

abstract：:It is now appreciated that mitochondria form tubular networks that adapt to the requirements of the cell by undergoing changes in their shape through fission and fusion. Proper mitochondrial distribution also appears to be required for ATP delivery and calcium regulation, and, in some cases, for cell development. Whil...

journal_title：Biological chemistry

authors： Frazier AE,Kiu C,Stojanovski D,Hoogenraad NJ,Ryan MT

更新日期：2006-12-01 00:00:00

abstract：:Vascular injury triggers endothelial exocytosis of granules, releasing pro-inflammatory and pro-thrombotic mediators into the blood. Nitric oxide (NO) and reactive oxygen species (ROS) limit vascular inflammation and thrombosis by inhibiting endothelial exocytosis. NO decreases exocytosis by regulating the activity of...

journal_title：Biological chemistry

authors： Lowenstein CJ,Tsuda H

更新日期：2006-10-01 00:00:00

abstract：:Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are the isoaspartate (isoAsp)-Abeta peptides and pyroglutamyl (pGlu)-Abeta. While pGl...

journal_title：Biological chemistry

authors： Böhme L,Hoffmann T,Manhart S,Wolf R,Demuth HU

更新日期：2008-08-01 00:00:00

abstract：:The cysteine-type peptidase cathepsin X is highly upregulated in several cancers and presumably promotes tumor invasion through bypassing cellular senescence. Here, we present first evidence that the underlying mechanism may involve the regulation of the insulin-like growth factor (IGF) system, a well-known activator ...

journal_title：Biological chemistry

authors： Kraus S,Fruth M,Bunsen T,Nägler DK

更新日期：2012-12-01 00:00:00

abstract：:Coxsackievirus B3 (CVB-3) is a plus-strand RNA virus that is believed to be the most common causal agent of viral myocarditis. Since no specific treatment for CVB-3 infections is available to date, we and others have recently started to develop RNA interference (RNAi) approaches to prevent virus propagation. Here we d...

journal_title：Biological chemistry

authors： Werk D,Schubert S,Lindig V,Grunert HP,Zeichhardt H,Erdmann VA,Kurreck J

更新日期：2005-09-01 00:00:00

abstract：:The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9-16 μmol/g resulting in nanomaterials with high local longitudinal and tra...

journal_title：Biological chemistry

authors： Joshi R,Feldmann V,Koestner W,Detje C,Gottschalk S,Mayer HA,Sauer MG,Engelmann J

更新日期：2013-01-01 00:00:00