Abstract:
:Mitochondrial abnormality in the skeletal muscles of 13 patients with myotonic dystrophy was analyzed by both histochemical and molecular biologic methods. Nine of 13 patients had ragged-red fibers (50 +/- 116 per 10,000 muscle fibers, mean +/- SD), and 10 patients had cytochrome c oxidase-negative fibers (41 +/- 90 per 10,000 muscle fibers). Southern blot analysis detected no mitochondrial DNA deletions, while PCR revealed multiple mitochondrial DNA deletions in all the specimens. Direct sequencing of one of the deleted mitochondrial DNAs disclosed that the junctional sequence of a 3,460-bp deletion involved a 6-bp directly repeated sequence (5'-TAGAAG-3') flanked by C-rich regions located on the CO3 gene and the ND5 gene. Quantitative analysis of PCR amplified deleted mitochondrial DNAs revealed that the amount of deleted mitochondrial DNAs had positive correlation both with the frequencies of ragged-red fibers and cytochrome c oxidase-negative fibers. Although deleted mitochondrial DNAs were observed even in controls above age 30, the mean amount of deleted mitochondrial DNAs in patients with myotonic dystrophy was significantly higher than in controls. Moreover, the increase of deleted mitochondrial DNAs with aging was more marked in myotonic dystrophy than in controls. These results suggest that increased mitochondrial DNA deletions and consequent impairment of mitochondrial function contribute to the pathophysiology of myotonic dystrophy.
journal_name
Gerontologyjournal_title
Gerontologyauthors
Sahashi K,Tanaka M,Tashiro M,Ohno K,Ibi T,Takahashi A,Ozawa Tdoi
10.1159/000213303keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
18-29issue
1-2eissn
0304-324Xissn
1423-0003journal_volume
38pub_type
杂志文章相关文献
GERONTOLOGY文献大全abstract::We studied the effects of factors associated with vascular injury on the production of endothelin-1 (ET-1) in cultured porcine aortic endothelial cells. ET-1 mRNA expression and peptide production in endothelial cells were increased by thrombin, transforming growth factor beta 1 (TGF beta 1), interleukin-1 (IL-1) and ...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000213360
更新日期:1992-01-01 00:00:00
abstract::To develop a new method for accurately predicting creatinine clearance (Ccr) in the debilitated elderly without urine collections, the total muscle volume of both thighs (MV thigh), as a new predictive parameter, was calculated using computed tomography in 68 debilitated patients aged 61-92 years. A regression line be...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000213705
更新日期:1995-01-01 00:00:00
abstract::Mechanisms underlying the decreased febrile response in the elderly have not been elucidated. One hypothesis is that elderly persons have a decreased response at the hypothalamic or peripheral effector level to endogenous pyrogen (EP). We studied the response to human monocyte-derived EP in young adult and aged Fische...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000212723
更新日期:1985-01-01 00:00:00
abstract::The life span of Drosophila melanogaster was reduced when copper compounds were fed at concentrations of 1 X 10(-3) M or greater. Feeding lower concentrations of copper compounds failed to significantly change life span. A combination of copper gluconate and gluconic acid increased the adult life span by 21.6%. We con...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000212611
更新日期:1984-01-01 00:00:00
abstract::The Zika virus (ZIKV) outbreak in French Polynesia, in 2013, and in Brazil, in 2015, was correlated with neurological complications, which comprised, among others, congenital microcephaly and Guillain-Barré syndrome (GBS), which includes a group of acute autoimmune neuropathies generally reported after respiratory or ...
journal_title:Gerontology
pub_type: 杂志文章,评审
doi:10.1159/000453579
更新日期:2017-01-01 00:00:00
abstract:UNLABELLED:BACKGROUND; The link between experiencing difficulties in reading or understanding and non-compliance with drug treatment among seniors is not clearly established. OBJECTIVES:We measured the effect of both difficulties in reading, as well as difficulties in understanding prescription labelling, on non-compl...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000048924
更新日期:2002-01-01 00:00:00
abstract::Dementia is a symptom of a variety of specific structural brain diseases as well as several system degenerations. Alzheimer's disease presently is the commonest cause in the developed world, causing a cortical-subcortical degeneration of ascending cholinergic neurons and large pyramidal cells in the cerebral cortex. C...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000212832
更新日期:1986-01-01 00:00:00
abstract::During a winter epidemic of A/H1N1 influenza virus, we evaluated the protection conferred by vaccination of 285 residents of a nursing home. Fifteen of 204 members of the nursing staff were also vaccinated. Serological determinations were performed before and after vaccination using radial hemolysis (RH) and neuramini...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000213520
更新日期:1993-01-01 00:00:00
abstract::We use nerve tissue cultures as a model system for studying the mode of action of centrally acting drugs. Neurons in cerebellar cultures retain a number of organ-specific properties including the formation of complex synaptic networks and characteristic bioelectric activity. Electrical activity may be recorded from Pu...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000212300
更新日期:1978-01-01 00:00:00
abstract::The article discusses some aspects demonstrating that a decrease in acetylcholine synthesis in senile dementia of the Alzheimer type (SDAT) is a consequence of the strong decline in glucose turnover in the brain. This becomes obvious by the fact that acetylcoenzyme A, the key substrate of acetylcholine synthesis, is e...
journal_title:Gerontology
pub_type: 杂志文章,评审
doi:10.1159/000213592
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:The link between longevity and diet is of great interest to biological and gerontological research. The fact that relevant knowledge has generally been available for many centuries is often not remarked upon. OBJECTIVE:This article examines three aspects of early modern Western medicine which thematize the ...
journal_title:Gerontology
pub_type: 历史文章,杂志文章
doi:10.1159/000082198
更新日期:2005-03-01 00:00:00
abstract::Form birefringence curves and linear dichroic properties of aging rat tendons are different from those of adult controls. The steepness of the form birefringence curves and the increased retardations found in aging tendons are ascribed to an increase in collagen aggregation accompanied by loss of tissue water. Based o...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000212314
更新日期:1979-01-01 00:00:00
abstract::Sera from 40 elderly individuals ranging in age from 60 to 94 years were tested for the presence of antibodies to varicella-zoster virus (VZV)-specific proteins. Sodium dodecylsulfate polyacrylamide gel electrophoresis analysis of lysates of VZV-infected BSC-1 cells labeled with either [35S]methionine or [3H]mannose a...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000212962
更新日期:1988-01-01 00:00:00
abstract::The experimental evidence is reviewed which bears on the theory that aging in mammalian cells may be related to a decline in the efficiency of normal DNA repair processes. Although the data are as yet fragmentary, they do suggest that there is an age-associated decline in the capacity of cells to perform at least cert...
journal_title:Gerontology
pub_type: 杂志文章,评审
doi:10.1159/000212123
更新日期:1976-01-01 00:00:00
abstract::The age-related accumulation of lipopigments was determined in the adrenergic and nonadrenergic peripheral neurons of the rat using a microspectrofluorometric method and electron microscopy, 3-, 12- and 30-month-old rats were used. The rate of lipopigment accumulation was greater in nonadrenergic than adrenergic neuro...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000212808
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:The chronological age is clearly the strongest risk factor for fractures or death. Age as a concept can be described exactly as chronological age. Age in relative terms can be described as biological age. OBJECTIVE:We postulated that, even without taking into account known or unknown comorbidity, an immedia...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000079129
更新日期:2004-09-01 00:00:00
abstract::We review findings on spousal interrelations in old age in such key domains as cognition, well-being, and health. Therein, we demonstrate that spousal interrelations may extend developmental options but may also make an individual vulnerable to the experience of loss. We address theoretical questions concerning possib...
journal_title:Gerontology
pub_type: 杂志文章,评审
doi:10.1159/000211948
更新日期:2009-01-01 00:00:00
abstract::Although it is usually assumed that Lamarckian inheritance does not and cannot occur, molecular mechanisms by which non-mutational changes acquired in one generation can be transmitted to the next are now known. These mechanisms involve changes in chromatin structure, rather than changes in DNA base sequence. It is ar...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000213217
更新日期:1990-01-01 00:00:00
abstract::(Na+-K+)-ATPase is an enzyme responsible for ionic homeostasis in excitable tissues. Thus, the activity of this enzyme is essential for the maintenance of optimal function of the cerebral cortex. Although abnormalities of cerebral oxidative metabolism have been described in aging, these abnormalities do not appear to ...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000213123
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:Previous extensive clinical studies, using angiography or ultrasound, investigated the influence of alimentary, medicamentous and other antiatherosclerotic measures on the degree of atherosclerosis. The following signs of regression were found: clinical improvement, changes in tonus of vascular walls, format...
journal_title:Gerontology
pub_type: 杂志文章,评审
doi:10.1159/000065248
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND:Recent studies have shown that vitamin D status may be relevant for physical and cognitive performance in the older population. This association may be of particular interest to older people at risk for cognitive impairment and functional decline. OBJECTIVE:The aim of this study was to determine the associa...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000355667
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:C-reactive protein (CRP), a nonspecific marker of the inflammatory status, is associated with cardiovascular disease risk factors and may be an important feature of the metabolic syndrome (MSX) in middle-aged subjects. OBJECTIVES:We assessed the relationship of CRP levels to specific components of MSX and o...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000103555
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Hypoxia and aging determine on mammalian cells a stress response which implies modified production of oxidants, reactive oxygen species or reactive nitrogen species at the mitochondrial level, interfering with cell-signaling proteins and inducing mitochondrial damage, apoptosis occurrence and functional cons...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000299105
更新日期:2010-01-01 00:00:00
abstract::Both ageing and vitamin C (VC) deficiency result in immune defect. Since low serum and tissue levels of VC are found in the elderly, we have in a placebo-controlled study, tested the effect of VC supplements (500 mg/day i.m. for 1 month) on various immune parameters. Indeed, VC enhances the proliferative response of T...
journal_title:Gerontology
pub_type: 临床试验,杂志文章
doi:10.1159/000213131
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:The perioperative assessment and management of elderly patients with hip fracture and significant aortic stenosis (AS) is an increasingly common clinical problem with little data available to guide perioperative management. OBJECTIVES:It was the aim of this study to examine the incidence of perioperative ev...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000209245
更新日期:2009-01-01 00:00:00
abstract::To evaluate the requirement for provocative cystometry in urodynamic investigation in the elderly with urinary incontinence, 100 consecutive patients, who were referred on the basis of defined clinical criteria, were first studied by supine cystometry followed by provocative tests where necessary. The most satisfactor...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000212791
更新日期:1986-01-01 00:00:00
abstract:AIM:To investigate acceptance and usage issues in relation to Telehealth products as used by frail older clients of the Transition Care Program and their carers. METHOD:The study design was a quasi-randomised controlled clinical trial. A stratified random sample of participants was allocated to one of five groups usin...
journal_title:Gerontology
pub_type: 杂志文章,随机对照试验
doi:10.1159/000335033
更新日期:2012-01-01 00:00:00
abstract::Morphometric measurements have been carried out on the human myenteric esophageal neurons at the ages of 20-40 and more than 70 years. The number of neurons decreases after 70 years of age, which is accompanied by an increase in the sizes of the neurons. In percentage terms the decrease in the number of neurons in the...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000213658
更新日期:1995-01-01 00:00:00
abstract::The kinetics of cell proliferation and sister chromatid exchanges (SCE) were studied in blood lymphocytes treated by mitomycin C (MMC) in young and old human donors. In both the young and old donors MMC inhibited cell proliferation. Spontaneous SCE frequency was not age-dependent. In young donors, MMC induced a dose-r...
journal_title:Gerontology
pub_type: 杂志文章
doi:10.1159/000212658
更新日期:1984-01-01 00:00:00
abstract::Different approaches using cell culture techniques to study the biology of aging are critically described. Most of the studies concerned the relationship between cell division potential and aging. The growth potential of cells is fundamental for aging of the organism, since it relates to phenomena such as the regenera...
journal_title:Gerontology
pub_type: 杂志文章,评审
doi:10.1159/000022135
更新日期:2000-03-01 00:00:00