Biogenesis of lysosome-related organelles complex 3 (BLOC-3): a complex containing the Hermansky-Pudlak syndrome (HPS) proteins HPS1 and HPS4.

abstract：:A striking and widespread observation is that higher-order folding for many RNAs is very slow, often requiring minutes. In some cases, slow folding reflects the need to disrupt stable, but incorrect, interactions. However, a molecular explanation for slow folding in most RNAs is unknown. The specificity domain of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mortimer SA,Weeks KM

更新日期：2009-09-15 00:00:00

abstract：:Ral (Ras-like) GTPases are directly activated by oncogenic Ras GTPases. Mutant K-Ras (G12C) has enabled the development of covalent K-Ras inhibitors currently in clinical trials. However, Ral, and the overwhelming majority of mutant oncogenic K-Ras, are devoid of a druggable pocket and lack an accessible cysteine for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bum-Erdene K,Liu D,Gonzalez-Gutierrez G,Ghozayel MK,Xu D,Meroueh SO

更新日期：2020-03-31 00:00:00

abstract：:DNA origami enables the precise fabrication of nanoscale geometries. We demonstrate an approach to engineer complex and reversible motion of nanoscale DNA origami machine elements. We first design, fabricate, and characterize the mechanical behavior of flexible DNA origami rotational and linear joints that integrate s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marras AE,Zhou L,Su HJ,Castro CE

更新日期：2015-01-20 00:00:00

abstract：:The acid-sensing ion channel 1a (ASIC1a) is abundantly expressed in the amygdala complex and other brain regions associated with fear. Studies of mice with a disrupted ASIC1 gene suggested that ASIC1a may contribute to learned fear. To test this hypothesis, we generated mice overexpressing human ASIC1a by using the pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wemmie JA,Coryell MW,Askwith CC,Lamani E,Leonard AS,Sigmund CD,Welsh MJ

更新日期：2004-03-09 00:00:00

abstract：:Copper is an essential metal nutrient that is tightly regulated in the body because loss of its homeostasis is connected to severe diseases such as Menkes and Wilson diseases, Alzheimer's disease, prion disorders, and amyotrophic lateral sclerosis. The complex relationships between copper status and various stages of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirayama T,Van de Bittner GC,Gray LW,Lutsenko S,Chang CJ

更新日期：2012-02-14 00:00:00

abstract：:Liver fibrosis interferes with normal liver function and facilitates hepatocellular carcinoma (HCC) development, representing a major threat to human health. Here, we present a comprehensive perspective of microRNA (miRNA) function on targeting the fibrotic microenvironment. Starting from a murine HCC model, we identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Winkler I,Bitter C,Winkler S,Weichenhan D,Thavamani A,Hengstler JG,Borkham-Kamphorst E,Kohlbacher O,Plass C,Geffers R,Weiskirchen R,Nordheim A

更新日期：2020-01-07 00:00:00

abstract：:Division of labor is fundamental to the success of all societies. The most striking examples are the physically polymorphic worker castes in social insects with clear morphological adaptations to different roles. These polymorphic worker castes have previously been thought to be a classic example of nongentically cont...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes WO,Sumner S,Van Borm S,Boomsma JJ

更新日期：2003-08-05 00:00:00

abstract：:A strain of Escherichia coli, selected on the basis of its resistance to colicin N, reveals distinct structural and functional alterations in unspecific OmpF porin. A single mutation [Gly-119-->Asp (G119D)] was identified in the internal loop L3 that contributes critically to the formation of the construction inside t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jeanteur D,Schirmer T,Fourel D,Simonet V,Rummel G,Widmer C,Rosenbusch JP,Pattus F,Pagès JM

更新日期：1994-10-25 00:00:00

abstract：:How did human cooperation evolve? Recent evidence shows that many people are willing to engage in altruistic punishment, voluntarily paying a cost to punish noncooperators. Although this behavior helps to explain how cooperation can persist, it creates an important puzzle. If altruistic punishment provides benefits to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fowler JH

更新日期：2005-05-10 00:00:00

abstract：:1-Aminocyclopropane-1-carboxylate (ACC) oxidase catalyzes the oxidation of ACC to ethylene. Following conventional column fractionation, the enzyme was purified 180-fold to near homogeneity with a specific activity of 20 nmol/(mg.min). This purified enzyme preparation migrated as a single protein band with an apparent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dong JG,Fernández-Maculet JC,Yang SF

更新日期：1992-10-15 00:00:00

abstract：:Mahler proved the following quantitative result supplementing the Lindemann-Weierstrass theorem: Sigma(i=0) (n)C(i)e(ri) > H(-n-epsilon) for any distinct rational numbers r(0),r(1),..., r(n) and rational integers C(0),C(1),...,C(n) with H = max(0

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chudnovsky GV

更新日期：1983-05-01 00:00:00

abstract：:Nucleotide excision repair proteins have been implicated in genetic recombination by experiments in Saccharomyces cerevisiae and Drosophila melanogaster, but their role, if any, in mammalian cells is undefined. To investigate the role of the nucleotide excision repair gene ERCC1, the hamster homologue to the S. cerevi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sargent RG,Rolig RL,Kilburn AE,Adair GM,Wilson JH,Nairn RS

更新日期：1997-11-25 00:00:00

abstract：:The problem of Congressional apportionment is explained together with a brief history of the methods used or considered for its solution. Reasons are given for rejecting the presently used method of equal proportions and for accepting a new method, the quota method, which is the unique method satisfying three essentia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balinski ML,Young HP

更新日期：1974-11-01 00:00:00

abstract：:When light-grown seedlings of amaranth are transferred to total darkness, synthesis of the large subunit (LS) and small subunit (SS) of ribulose-1,5-bisphosphate carboxylase [RbuP(2)Case; 3-phospho-D-glycerate carboxylase (dimerizing), EC 4.1.1.39] is rapidly depressed. This reduction in RbuP(2)Case synthesis occurs i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berry JO,Carr JP,Klessig DF

更新日期：1988-06-01 00:00:00

abstract：:Protozoan parasites of the genus Leishmania undergo a complex life cycle involving transmission by biting sand flies and replication within mammalian macrophage phagolysosomes. A major component of the Leishmania surface coat is the glycosylphosphatidylinositol (GPI)-anchored polysaccharide called lipophosphoglycan (L...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Späth GF,Epstein L,Leader B,Singer SM,Avila HA,Turco SJ,Beverley SM

更新日期：2000-08-01 00:00:00

abstract：:Paired immunoglobulin-like type 2 receptor α (PILRα) and β (PILRβ) belong to the PILR family and are related to innate immune regulation in various species. Despite their high sequence identity, PILRα and PILRβ are shown to have variant sialic acid (SA) binding avidities. To explore the molecular basis of this interac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu Q,Lu G,Qi J,Wang H,Xuan Y,Wang Q,Li Y,Zhang Y,Zheng C,Fan Z,Yan J,Gao GF

更新日期：2014-06-03 00:00:00

abstract：:Lateral inhibition is the ubiquitous strategy used by visual neurons for spatial resolution throughout the animal kingdom. It has been a puzzle whether lateral inputs in retinal bipolar cells are mediated by the horizontal cell (HC)-cone feedback synapse, by the HC-bipolar cell feedforward synapse, or by both. By bloc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang XL,Wu SM

更新日期：1991-04-15 00:00:00

abstract：:Bves is an integral membrane protein with no determined function and no homology to proteins outside of the Popdc family. It is widely expressed throughout development in myriad organisms. Here, we demonstrate an interaction between Bves and guanine nucleotide exchange factor T (GEFT), a GEF for Rho-family GTPases. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith TK,Hager HA,Francis R,Kilkenny DM,Lo CW,Bader DM

更新日期：2008-06-17 00:00:00

abstract：:The reduction in levels of the potentially toxic amyloid-beta peptide (Abeta) has emerged as one of the most important therapeutic goals in Alzheimer's disease. Key targets for this goal are factors that affect the expression and processing of the Abeta precursor protein (betaAPP). Earlier reports from our laboratory ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaw KT,Utsuki T,Rogers J,Yu QS,Sambamurti K,Brossi A,Ge YW,Lahiri DK,Greig NH

更新日期：2001-06-19 00:00:00

abstract：:Pheromone biosynthesis-activating neuropeptide (PBAN) regulates sex pheromone biosynthesis in female Helicoverpa (Heliothis) zea. Two oligonucleotide probes representing two overlapping amino acid regions of PBAN were used to screen 2.5 x 10(5) recombinant plaques, and a positive recombinant clone was isolated. Sequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis MT,Vakharia VN,Henry J,Kempe TG,Raina AK

更新日期：1992-01-01 00:00:00

abstract：:The structure of cellulose microfibrils in wood is not known in detail, despite the abundance of cellulose in woody biomass and its importance for biology, energy, and engineering. The structure of the microfibrils of spruce wood cellulose was investigated using a range of spectroscopic methods coupled to small-angle ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fernandes AN,Thomas LH,Altaner CM,Callow P,Forsyth VT,Apperley DC,Kennedy CJ,Jarvis MC

更新日期：2011-11-22 00:00:00

abstract：:Knowledge of the range and chronology of historic trade and long-distance transport of natural resources is essential for determining the impacts of past human activities on marine environments. However, the specific biological sources of imported fauna are often difficult to identify, in particular if species have a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Star B,Boessenkool S,Gondek AT,Nikulina EA,Hufthammer AK,Pampoulie C,Knutsen H,André C,Nistelberger HM,Dierking J,Petereit C,Heinrich D,Jakobsen KS,Stenseth NC,Jentoft S,Barrett JH

更新日期：2017-08-22 00:00:00

abstract：:A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaptain S,Downey WE,Tang C,Philpott C,Haile D,Orloff DG,Harford JB,Rouault TA,Klausner RD

更新日期：1991-11-15 00:00:00

abstract：:Sympatric speciation has been controversial since it was first proposed as a mode of speciation. Subterranean blind mole rats (Spalacidae) are considered to speciate allopatrically or peripatrically. Here, we report a possible incipient sympatric adaptive ecological speciation in Spalax galili (2n = 52). The study mic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hadid Y,Tzur S,Pavlícek T,Šumbera R,Šklíba J,Lövy M,Fragman-Sapir O,Beiles A,Arieli R,Raz S,Nevo E

更新日期：2013-02-12 00:00:00

abstract：:A plasmid construct expressing the p66 version of the human immunodeficiency virus reverse transcriptase as a bacterial fusion protein was subjected to in vitro mutagenesis, and the resulting variant proteins were assayed to define the locations of the two major enzymatic activities. The DNA polymerase activity was lo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prasad VR,Goff SP

更新日期：1989-05-01 00:00:00

abstract：:A high proportion of lymphocytes from F/St mice produce infectious xenotropic murine leukemia virus (X-MuLV) and express high levels of cell surface antigens, termed XenCSA, related to the major glycoprotein of X-MuLV. In crosses of F/St with AKR, the high-virus phenotype of F/St was found to be recessive and was show...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yetter RA,Hartley JW,Morse HC 3rd

更新日期：1983-01-01 00:00:00

abstract：:Using morphometrical methods, we investigated variation in the skeletons of more than 400 offspring of C57BL/6J and DBA/2J male mice that had received either a 250 mg/kg dose of the mutagen ethylnitrosourea or a solvent control. Sperm involved in the matings developed from cells in the spermatogonial stage at the time...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lovell DP,Willis DB,Johnson FM

更新日期：1985-05-01 00:00:00

abstract：:Interstitial telomeric sequences (ITSs) are present in many eukaryotic genomes and are linked to genome instabilities and disease in humans. The mechanisms responsible for ITS-mediated genome instability are not understood in molecular detail. Here, we use a model Saccharomyces cerevisiae system to characterize genome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aksenova AY,Greenwell PW,Dominska M,Shishkin AA,Kim JC,Petes TD,Mirkin SM

更新日期：2013-12-03 00:00:00

abstract：:Escherichia coli fMet-tRNAfMet alters the pattern of promoter selection of E. coli RNA polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6), affecting RNA synthesis from the rRNA, suIII+tRNA, and lac promoters in different ways. The in vitro synthesis of the stable RNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Debenham PG,Pongs O,Travers AA

更新日期：1980-02-01 00:00:00

abstract：:The emergence of antibiotic resistance among nosocomial pathogens has reemphasized the need for effective infection control strategies. The spread of resistant pathogens within hospital settings proceeds along various routes of transmission and is characterized by large fluctuations in prevalence, which are typical fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pelupessy I,Bonten MJ,Diekmann O

更新日期：2002-04-16 00:00:00