Abstract:
:Escherichia coli fMet-tRNAfMet alters the pattern of promoter selection of E. coli RNA polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase; EC 2.7.7.6), affecting RNA synthesis from the rRNA, suIII+tRNA, and lac promoters in different ways. The in vitro synthesis of the stable RNA species is selectively decreased, whereas that of lac RNA from both the wild-type and mutant UV5 promoters is selectively increased at high ionic strength. The functional effect of fMet-tRNAfMet resembles that of the nucleotide guanosine 3'-diphosphate 5'-diphosphate (ppGpp). This nucleotide competes with the binding of fMet-tRnafMet to RNA polymerase.
journal_name
Proc Natl Acad Sci U S Aauthors
Debenham PG,Pongs O,Travers AAdoi
10.1073/pnas.77.2.870subject
Has Abstractpub_date
1980-02-01 00:00:00pages
870-4issue
2eissn
0027-8424issn
1091-6490journal_volume
77pub_type
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