当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > C2'-endo nucleotides as molecular timers suggested by the folding of an RNA domain.

C2'-endo nucleotides as molecular timers suggested by the folding of an RNA domain.

Abstract：

:A striking and widespread observation is that higher-order folding for many RNAs is very slow, often requiring minutes. In some cases, slow folding reflects the need to disrupt stable, but incorrect, interactions. However, a molecular explanation for slow folding in most RNAs is unknown. The specificity domain of the Bacillus subtilis RNase P ribozyme undergoes a rate-limiting folding step on the minute time-scale. This RNA also contains a C2'-endo nucleotide at A130 that exhibits extremely slow local conformational dynamics. This nucleotide is evolutionarily conserved and essential for tRNA recognition by RNase P. Here we show that deleting this single nucleotide accelerates folding by an order of magnitude even though this mutation does not change the global fold of the RNA. These results demonstrate that formation of a single stacking interaction at a C2'-endo nucleotide comprises the rate-determining step for folding an entire 154 nucleotide RNA. C2'-endo nucleotides exhibit slow local dynamics in structures spanning isolated helices to complex tertiary interactions. Because the motif is both simple and ubiquitous, C2'-endo nucleotides may function as molecular timers in many RNA folding and ligand recognition reactions.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Mortimer SA,Weeks KM

doi

10.1073/pnas.0901319106

subject

Has Abstract

pub_date

2009-09-15 00:00:00

pages

15622-7

issue

37

eissn

0027-8424

issn

1091-6490

pii

0901319106

journal_volume

106

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • A rapid and label-free platform for virus capture and identification from clinical samples.

    abstract：:Emerging and reemerging viruses are responsible for a number of recent epidemic outbreaks. A crucial step in predicting and controlling outbreaks is the timely and accurate characterization of emerging virus strains. We present a portable microfluidic platform containing carbon nanotube arrays with differential filtra...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1910113117

    authors： Yeh YT,Gulino K,Zhang Y,Sabestien A,Chou TW,Zhou B,Lin Z,Albert I,Lu H,Swaminathan V,Ghedin E,Terrones M

    更新日期：2020-01-14 00:00:00

  • Common cell-surface antigen associated with murine and feline C-type RNA leukemia viruses.

    abstract：:A new common cell-surface antigen associated with murine and feline C-type RNA leukemia viruses was demonstrated by the use of rabbit antiserum against feline leukemia virus and the indirect membrane immunofluorescence test. Common cell-surface antigen was found in all leukemias of all strains of mice tested, in norma...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.70.6.1878

    authors： Yoshiki T,Mellors RC,Hardy WD Jr

    更新日期：1973-06-01 00:00:00

  • Pyrolysis of organomercury compounds: investigation by the method of matrix isolation.

    abstract：:The method of matrix isolation has been used to investigate mechanisms of gas-phase chemical reactions, in particular the pyrolysis of some organomercury compounds. A molecular beam of pyrolysis products was condensed simultaneously with a large excess of rare gas at temperatures from 5 to 15 degrees K to form a matri...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.68.12.3238

    authors： Maltsev AK,Mikaelian RG,Nefedov OM,Hauge RH,Margrave JL

    更新日期：1971-12-01 00:00:00

  • Fluorescence-activated sorting of totipotent embryonic stem cells expressing developmentally regulated lacZ fusion genes.

    abstract：:Murine embryonic stem (ES) cells were infected with a retrovirus promoter trap vector, and clones expressing lacZ fusion genes (LacZ+) were isolated by fluorescence-activated cell sorting (FACS). Of 12 fusion genes tested, 1 was repressed when ES cells were allowed to differentiate in vitro. Two of three lacZ fusion g...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.15.6721

    authors： Reddy S,Rayburn H,von Melchner H,Ruley HE

    更新日期：1992-08-01 00:00:00

  • Photoperiodic information acquired and stored in vivo is retained in vitro by a circadian oscillator, the avian pineal gland.

    abstract：:Endogenous circadian rhythms have been described in a wide range of organisms from prokaryotes to man. Although basic circadian mechanisms at the molecular level are genetically fixed, certain properties of circadian rhythms at the organismic level can be modified by environmental conditions and subsequently retained ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.200354997

    authors： Brandstätter R,Kumar V,Abraham U,Gwinner E

    更新日期：2000-10-24 00:00:00

  • An enzymatic defect in the obese (ob/ob) mouse: loss of thyroid-induced sodium- and potassium-dependent adenosinetriphosphatase.

    abstract：:Genetically obese (ob/ob) mice, mice that became obese after treatment with gold thioglucose, and lean animals were studied in the euthyroid state, after induction of hypothyroidism, and after treatment with triiodothyronine. The activity of glycerol 3-phosphate dehydrogenase (sn-glycerol-3-phosphate:(acceptor) oxidor...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.1.477

    authors： York DA,Bray GA,Yukimura Y

    更新日期：1978-01-01 00:00:00

  • In situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: an approach to the diagnosis of viral heart disease.

    abstract：:We have developed an in situ hybridization assay capable of detecting enteroviral RNA in myocardial cells, using molecularly cloned coxsackievirus B3 cDNA as a diagnostic probe. Because of the high degree of nucleic acid sequence homology among the numerous enteroviral serotypes, including the group A and B coxsackiev...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.17.6272

    authors： Kandolf R,Ameis D,Kirschner P,Canu A,Hofschneider PH

    更新日期：1987-09-01 00:00:00

  • Dual role of Brg chromatin remodeling factor in Sonic hedgehog signaling during neural development.

    abstract：:Sonic hedgehog (Shh) signaling plays diverse roles during animal development and adult tissue homeostasis through differential regulation of Gli family transcription factors. Dysregulated Shh signaling activities have been linked to birth defects and tumorigenesis. Here we report that Brg, an ATP-dependent chromatin r...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1018510108

    authors： Zhan X,Shi X,Zhang Z,Chen Y,Wu JI

    更新日期：2011-08-02 00:00:00

  • Periodic and chaotic host-parasite interactions in human malaria.

    abstract：:It has been recognized since ancient times that malaria fever is highly periodic but the mechanism has been poorly understood. Malaria fever is related to the parasite growth cycle in erythrocytes. After a fixed period of replication, a mature parasite (schizont) causes the infected erythrocyte to rupture, releasing p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.12.5111

    authors： Kwiatkowski D,Nowak M

    更新日期：1991-06-15 00:00:00

  • Endothelial plasmalemmal vesicles as elements in a system of branching invaginations from the cell surface.

    abstract：:In electron microscopy studies of the endothelial vesicles in frog mesenteric capillaries, an accidental observation was made concerning vesicular organization. When tannic acid was added to already fixed tissue, the mordant reached apparently free vesicles in the cytoplasm under conditions in which vesicular movement...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.12.6439

    authors： Bundgaard M,Frøkjaer-Jensen J,Crone C

    更新日期：1979-12-01 00:00:00

  • Bis-(3'-5')-cyclic dimeric GMP-linked quorum sensing controls swarming in Vibrio parahaemolyticus.

    abstract：:Movement over and colonization of surfaces are important survival strategies for bacteria, and many find it advantageous to perform these activities as a group, using quorum sensing to sample population size and synchronize behavior. It is puzzling however, that swarming-proficient and virulent strains of Vibrio parah...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1113790108

    authors： Trimble MJ,McCarter LL

    更新日期：2011-11-01 00:00:00

  • A mutation in the peroxisome proliferator-activated receptor gamma-binding site in the gene for the cytosolic form of phosphoenolpyruvate carboxykinase reduces adipose tissue size and fat content in mice.

    abstract：:Regulation of the turnover of triglycerides in adipose tissue requires the continuous provision of 3-glycerophosphate, which may be supplied by the metabolism of glucose or by glyceroneogenesis, the de novo synthesis of 3-glycerophosphate from sources other than hexoses or glycerol. The importance of glyceroneogenesis...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.022616299

    authors： Olswang Y,Cohen H,Papo O,Cassuto H,Croniger CM,Hakimi P,Tilghman SM,Hanson RW,Reshef L

    更新日期：2002-01-22 00:00:00

  • Genetic analysis of traditional and evolved Basmati and non-Basmati rice varieties by using fluorescence-based ISSR-PCR and SSR markers.

    abstract：:The objective of the present study was to make use of efficient molecular marker systems to reveal genetic relationships in traditional and evolved Basmati (EB) and semidwarf non-Basmati (NB) rice varieties. A subset of three rice groups was analyzed by using 19 simple sequence repeat (SSR) loci and 12 inter-SSR-PCR p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.042099099

    authors： Nagaraju J,Kathirvel M,Kumar RR,Siddiq EA,Hasnain SE

    更新日期：2002-04-30 00:00:00

  • Success or failure of vaccination for HPV16-positive vulvar lesions correlates with kinetics and phenotype of induced T-cell responses.

    abstract：:One half of a group of 20 patients with human papillomavirus type 16 (HPV16)-induced vulvar intraepithelial neoplasia grade 3 displayed a complete regression (CR) after therapeutic vaccination with HPV16 E6/E7 synthetic long peptides. Patients with relatively larger lesions generally did not display a CR. To investiga...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1006500107

    authors： Welters MJ,Kenter GG,de Vos van Steenwijk PJ,Löwik MJ,Berends-van der Meer DM,Essahsah F,Stynenbosch LF,Vloon AP,Ramwadhdoebe TH,Piersma SJ,van der Hulst JM,Valentijn AR,Fathers LM,Drijfhout JW,Franken KL,Oostendorp J,Fle

    更新日期：2010-06-29 00:00:00

  • Complete amino acid sequence and homologies of human erythrocyte membrane protein band 4.2.

    abstract：:The complete amino acid sequence for human erythrocyte band 4.2 has been derived from the nucleotide sequence of a full-length 2.35-kilobase (kb) cDNA. The 2.35-kb cDNA was isolated from a human reticulocyte cDNA library made in the expression vector lambda gt11. Of the 2348 base pairs (bp), 2073 bp encode 691 amino a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.2.613

    authors： Korsgren C,Lawler J,Lambert S,Speicher D,Cohen CM

    更新日期：1990-01-01 00:00:00

  • Structural implications of sequence variability in immunoglobulins.

    abstract：:Immunoglobulin sequences were compared by using a technique that takes into account the dissimilarity in physicochemical properties of amino acids. Exterior residues showed greater structural variability than interior residues. High structural variability was found at positions known from crystallographic studies to b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.6.2551

    authors： Padlan EA

    更新日期：1977-06-01 00:00:00

  • Global temperature responses to current emissions from the transport sectors.

    abstract：:Transport affects climate directly and indirectly through mechanisms that cause both warming and cooling of climate, and the effects operate on very different timescales. We calculate climate responses in terms of global mean temperature and find large differences between the transport sectors with respect to the size...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0804844105

    authors： Berntsen T,Fuglestvedt J

    更新日期：2008-12-09 00:00:00

  • Genome sequencing of environmental Escherichia coli expands understanding of the ecology and speciation of the model bacterial species.

    abstract：:Defining bacterial species remains a challenging problem even for the model bacterium Escherichia coli and has major practical consequences for reliable diagnosis of infectious disease agents and regulations for transport and possession of organisms of economic importance. E. coli traditionally is thought to live with...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1015622108

    authors： Luo C,Walk ST,Gordon DM,Feldgarden M,Tiedje JM,Konstantinidis KT

    更新日期：2011-04-26 00:00:00

  • C-terminal kink formation is required for lateral gating in BamA.

    abstract：:In Gram-negative bacteria, the outer membrane contains primarily β-barrel transmembrane proteins and lipoproteins. The insertion and assembly of β-barrel outer-membrane proteins (OMPs) is mediated by the β-barrel assembly machinery (BAM) complex, the core component of which is the 16-stranded transmembrane β-barrel Ba...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1722530115

    authors： Lundquist K,Bakelar J,Noinaj N,Gumbart JC

    更新日期：2018-08-21 00:00:00

  • A role for the melanocortin 4 receptor in sexual function.

    abstract：:By using a combination of genetic, pharmacological, and anatomical approaches, we show that the melanocortin 4 receptor (MC4R), implicated in the control of food intake and energy expenditure, also modulates erectile function and sexual behavior. Evidence supporting this notion is based on several findings: (i) a high...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.172378699

    authors： Van der Ploeg LH,Martin WJ,Howard AD,Nargund RP,Austin CP,Guan X,Drisko J,Cashen D,Sebhat I,Patchett AA,Figueroa DJ,DiLella AG,Connolly BM,Weinberg DH,Tan CP,Palyha OC,Pong SS,MacNeil T,Rosenblum C,Vongs A,Tang R

    更新日期：2002-08-20 00:00:00

  • Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes.

    abstract：:Fluorescence in situ hybridization (FISH) is a powerful tool for physical mapping in human and other mammalian species. However, application of the FISH technique has been limited in plant species, especially for mapping single- or low-copy DNA sequences, due to inconsistent signal production in plant chromosome prepa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.10.4487

    authors： Jiang J,Gill BS,Wang GL,Ronald PC,Ward DC

    更新日期：1995-05-09 00:00:00

  • Rescue of a primary myelofibrosis model by retinoid-antagonist therapy.

    abstract：:Molecular targeting of the two receptor interaction domains of the epigenetic repressor silencing mediator of retinoid and thyroid hormone receptors (SMRT(mRID)) produced a transplantable skeletal syndrome that reduced radial bone growth, increased numbers of bone-resorbing periosteal osteoclasts, and increased bone f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1318974110

    authors： Hong SH,Dvorak-Ewell M,Stevens HY,Barish GD,Castro GL,Nofsinger R,Frangos JA,Shoback D,Evans RM

    更新日期：2013-11-19 00:00:00

  • Human placental alkaline phosphatase in liver and intestine.

    abstract：:Three distinct forms of human alkaline phosphatase, presumably isozymes, are known, each apparently associated with a specific tissue. These are placental, intestinal, and liver (kidney and bone). We have used a specific immunoassay and HPLC to show that placental alkaline phosphatase is also present in extracts of li...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.18.6080

    authors： Garattini E,Margolis J,Heimer E,Felix A,Udenfriend S

    更新日期：1985-09-01 00:00:00

  • Macropredatory ichthyosaur from the Middle Triassic and the origin of modern trophic networks.

    abstract：:The biotic recovery from Earth's most severe extinction event at the Permian-Triassic boundary largely reestablished the preextinction structure of marine trophic networks, with marine reptiles assuming the predator roles. However, the highest trophic level of today's marine ecosystems, i.e., macropredatory tetrapods ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 历史文章,杂志文章

    doi：10.1073/pnas.1216750110

    authors： Fröbisch NB,Fröbisch J,Sander PM,Schmitz L,Rieppel O

    更新日期：2013-01-22 00:00:00

  • Insulin stimulates a membrane-bound serine kinase that may be phosphorylated on tyrosine.

    abstract：:Triton X-100-solubilized high-density microsomes from insulin-treated rat adipocytes exhibit a marked increase in serine/threonine and tyrosine kinase activities toward exogenous histone when compared to controls. The insulin-dependent activation of microsomal histone kinase activities occurs within the physiological ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.12.3972

    authors： Yu KT,Khalaf N,Czech MP

    更新日期：1987-06-01 00:00:00

  • Torsional stress induces an S1 nuclease-hypersensitive site within the promoter of the Xenopus laevis oocyte-type 5S RNA gene.

    abstract：:The internal promoter of the Xenopus laevis oocyte-type 5S RNA gene is preferentially cleaved by S1 and Bal-31 nucleases in plasmid DNA. S1 nuclease sensitivity is largely dependent on supercoiling; however, Bal-31 cleaves within the 5S RNA gene in linear as well as in supercoiled DNA. The S1 nuclease-hypersensitive s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.12.4018

    authors： Reynolds WF,Gottesfeld JM

    更新日期：1985-06-01 00:00:00

  • Nematic twist-bend phase in an external field.

    abstract：:The response of the nematic twist-bend ([Formula: see text]) phase to an applied field can provide important insight into the structure of this liquid and may bring us closer to understanding mechanisms generating mirror symmetry breaking in a fluid of achiral molecules. Here we investigate theoretically how an extern...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1721786115

    authors： Pająk G,Longa L,Chrzanowska A

    更新日期：2018-10-30 00:00:00

  • Observing the formation of ice and organic crystals in active sites.

    abstract：:Heterogeneous nucleation is vital to a wide range of areas as diverse as ice nucleation on atmospheric aerosols and the fabrication of high-performance thin films. There is excellent evidence that surface topography is a key factor in directing crystallization in real systems; however, the mechanisms by which nanoscal...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1617717114

    authors： Campbell JM,Meldrum FC,Christenson HK

    更新日期：2017-01-31 00:00:00

  • The role of seawater endocytosis in the biomineralization process in calcareous foraminifera.

    abstract：:Foraminifera are unicellular organisms that inhabit the oceans in various ecosystems. The majority of the foraminifera precipitate calcitic shells and are among the major CaCO(3) producers in the oceans. They comprise an important component of the global carbon cycle and also provide valuable paleoceanographic informa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0906636106

    authors： Bentov S,Brownlee C,Erez J

    更新日期：2009-12-22 00:00:00

  • Immune response to p53 is dependent upon p53/HSP70 complexes in breast cancers.

    abstract：:Overexpression of the p53 protein, resulting from gene mutations that increase protein stability, has been detected in greater than 25% of primary human breast cancers. In addition, approximately 10% of breast cancer patients have circulating antibodies to the p53 protein. In this study, the anti-p53 humoral response ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.8.3439

    authors： Davidoff AM,Iglehart JD,Marks JR

    更新日期：1992-04-15 00:00:00