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Nuclear delivery of p53 C-terminal peptides into cancer cells using scFv fragments of a monoclonal antibody that penetrates living cells.

Abstract：

:scFv fragments of a monoclonal antibody that penetrates living cells and localizes in nuclei were designed as fusion proteins with C-terminal p53 peptides and tested for restoring p53 function in p53 mutant cancer cells. scFv fragments transported a 30-mer C-terminal peptide of p53 into cancer cells and induced cellular cytotoxicity in contrast to scFv fragments alone and other scFv-p53 fusion peptides. Cellular toxicity was not observed with scFv fragments containing a single mutation in VH that prevented antibody penetration. Our results demonstrate the potential efficacy of antibody scFv fragments as a nuclear delivery system in cancer cells.

journal_name

Cancer Lett

journal_title

Cancer letters

authors

Weisbart RH,Miller CW,Chan G,Wakelin R,Ferreri K,Koeffler HP

doi

10.1016/s0304-3835(03)00151-4

keywords:

subject

Has Abstract

pub_date

2003-06-10 00:00:00

pages

211-9

issue

2

eissn

0304-3835

issn

1872-7980

pii

S0304383503001514

journal_volume

195

pub_type

杂志文章

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