Abstract：
:The iron carrier protein transferrin plays a prominent antioxidant and anti-bacterial role in the lower respiratory tract and is present at elevated concentrations in lung epithelial lining fluid relative to plasma. The level of transferrin receptor synthesis in primary cultures of rat alveolar epithelial cells (AECs) was investigated. Transferrin receptor was found to be synthesized early in AEC cultures with the alveolar type II cell-like phenotype. Cell-surface receptor localization was attenuated upon apparent transdifferentiation to the alveolar type I cell-like phenotype later in culture. Binding of (125)I-labeled transferrin to the receptor indicated that surface and total cellular transferrin receptor levels were decreased in the type I-like cells. Inclusion of keratinocyte growth factor (KGF) in culture media (10 ng/ml) resulted in retention of transferrin receptor localized to the basolateral surface. Transferrin-receptor-specific internalization of (59)Fe-transferrin was also limited to the basolateral surface of KGF-treated monolayers. These data suggest that alveolar type II (but not type I) cells express functional transferrin receptor in adult rat alveolar epithelium.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Widera A,Beloussow K,Kim KJ,Crandall ED,Shen WCdoi
10.1007/s00441-002-0663-3keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
313-8issue
3eissn
0302-766Xissn
1432-0878journal_volume
312pub_type
杂志文章abstract：:Fragments of pituitary tissue obtained from a total of 37 patients with either breast cancer, diabetic retinopathy, galactorrhea, or acromegaly were dissociated into single cell suspensions prior to cell culture. Release of human growth hormone (hGH) and human prolactin (hPRL) into the culture medium was measured by r...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219803
更新日期：1978-08-16 00:00:00
abstract：:As a companion to an earlier study, the morphometric attributes of stimulated (blast-derived) lymphocytes in mouse axillary lymph nodes have been established using stereological and autoradiographic methods. Blast transformation was induced in vivo with dinitrochlorobenzene (DNCB) and stimulated cells were labelled wi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00233538
更新日期：1981-01-01 00:00:00
abstract：:Periodontal ligament (PDL) stem cell properties are critical in the periodontal tissue regeneration for periodontitis. Previously, we have demonstrated that cigarette smoking attenuates PDL-derived stem cell (PDLSC) regenerative properties. Here, we report the findings on the regenerative properties of human PDLSCs wi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-020-03176-y
更新日期：2020-07-01 00:00:00
abstract：:The electron-dense tracers ferritin, and iron-dextran, and the protein horseradish peroxidase, have been used to investigate the ultrastructural basis of permeability in the upper and lower segments of the Malpighian tubules of Glomeris marginata. All these materials were able to cross the basal lamina and enter the t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00219837
更新日期：1977-09-05 00:00:00
abstract：:The effect of a physiological dose of vitamin C (100 microg/ml) on goat articular cartilage chondrocytes cultured in an alginate matrix and subjected to static pressurization of 2.4 MPa was investigated. Biochemical analyses of DNA, glycosaminoglycan (GAG), collagen and protease activity were carried out in various ma...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-008-0666-9
更新日期：2008-10-01 00:00:00
abstract：:The fine structure of luminal surface of clearly identified portions of uriniferous tubules has been studied by scanning electron microscopy to elucidate some controversies concerning the topography of certain surface formations. The results show a characteristic pattern of the luminal surface in the region of Henle's...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215128
更新日期：1976-02-06 00:00:00
abstract：:Aquaporins (AQPs) are integral membrane proteins that function as channels for the transfer of water and small solutes across membranes. In mammalian cells, 13 isoforms (AQP0-12) have been identified, and these exhibit unique patterns of expression in various cell types and tissues. Among these isoforms, AQP6 is consi...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0558-4
更新日期：2008-04-01 00:00:00
abstract：:By means of a preembedding immuno-electron-microscopic technique, a large number of nerve endings containing a substance related to human growth hormone-releasing factor (hGRF) have been demonstrated in the paraventricular nucleus of the guinea pig. They made synaptic contacts primarily with dendritic shafts: 80% of t...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF01239984
更新日期：1987-04-01 00:00:00
abstract：:The number of cells comprising biological structures represents fundamental information in basic anatomy, development, aging, drug tests, pathology and genetic manipulations. Obtaining unbiased estimates of cell numbers, however, was until recently possible only through stereological techniques, which require specific...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-015-2127-6
更新日期：2015-04-01 00:00:00
abstract：:Zebrafish (Danio rerio) is widely used as an animal model to understand the pathophysiology of cardiovascular diseases. Here, we present the adult cardiac phenotype of weak atrium, myh6-/-, which carry mutations in the zebrafish atrial myosin heavy chain. Homozygous mutants survive to adulthood and are fertile despite...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-019-03044-4
更新日期：2019-11-01 00:00:00
abstract：:We have recently examined the electrophysiology and ultrastructure of approximately 100 tactile spines from the metathoracic legs of adult cockroaches. In only one animal the single sensory neuron that innervates the spine was replaced with a pair of apparently identical neurons which we believe were both functional. ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216522
更新日期：1984-01-01 00:00:00
abstract：:The complex physiology of the gastrointestinal (GI) tract must permanently be adjusted according to the composition of ingested food, which requires continuous monitoring by appropriate sensory systems. Sensing the dietary constituents is thought to be mediated by chemosensory cells residing in the mucosa of the GI tr...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-011-1137-2
更新日期：2011-05-01 00:00:00
abstract：:Unique rod-shaped secretory granules were observed among oval or spherical secretory granules in GH cells of the anterior pituitary gland of musk shrew using the protein A-gold procedure combined with electron microscopy. The rod-shaped and spherical secretory granules were both immunoreactive by the immuno-gold metho...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00215454
更新日期：1988-01-01 00:00:00
abstract：:Our previous findings have demonstrated that the rat autosomal-recessive mutation, whitish chalk-like teeth (wct), induces enamel defects resembling those of human amelogenesis imperfecta (AI) in continuously growing incisor teeth. The present study clarifies the effect of the wct mutation on the morphogenesis and cal...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-007-0452-0
更新日期：2007-10-01 00:00:00
abstract：:Somatostatin-like immunoreactivity was localized in nerve cell bodies and nerve terminals in the cat coeliac ganglion. Two types of somatostatin-immunoreactive cell bodies were revealed, the first being large (diameter 35 microns), numerous and weakly labelled, whereas the second was considerably smaller (diameter 10....
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00327290
更新日期：1991-03-01 00:00:00
abstract：:Proliferating cell nuclear antigen is expressed in cells from late G1 through the S-phase of the cell cycle. Therefore, antibodies directed against this molecule should provide a probe for labeling immunocytochemically the nuclei of proliferating cells. Herein we demonstrate the feasibility and reliability of this tec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00224731
更新日期：1989-04-01 00:00:00
abstract：:Dorsal unpaired median (DUM) neurones in the abdominal ganglia of the locust were impaled with microelectrodes and some were injected intracellularly with horseradish peroxidase so that their synapses could be identified in the electron microscope. Simultaneous recordings from DUM neurones in different abdominal gangl...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00307805
更新日期：1995-05-01 00:00:00
abstract：:The immunoreactive CRF-neurons of the rat hypothalamus have been examined immunohistochemically employing anti-rat CRF serum. These neurons are confined to the paraventricular nucleus, dorsomedial-lateral hypothalamic area, and suprachiasmatic nucleus, and are, respectively, also immunoreactive to anti-Met-enk, -alpha...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00216347
更新日期：1985-01-01 00:00:00
abstract：:Angiogenesis, the growth of new blood vessels, is required for a variety of normal proliferative processes. Furthermore, angiogenesis is well established as also playing an important role in neoplastic growth and metastasis. Numerous regulators of angiogenesis have been identified and characterized over the last few d...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-008-0675-8
更新日期：2009-01-01 00:00:00
abstract：:Immunoreactivity to the polypeptide designated "7B2" recently isolated from human and porcine pituitary glands, appears to be consistently confined to neuroendocrine and endocrine cells in various tissues. In rat gut, immunoreactive 7B2 was found in endocrine-paracrine cells. Highly labeled cells were found in the ant...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217343
更新日期：1987-09-01 00:00:00
abstract：:The amygdala is a central hub for fear learning assessed by Pavlovian fear conditioning. Indeed, the prevailing hypothesis that learning and memory are mediated by changes in synaptic strength was shown most convincingly at thalamic and cortical afferents to the lateral amygdala. The neurotrophin brain-derived neurotr...
journal_title：Cell and tissue research
pub_type： 杂志文章,评审
doi：10.1007/s00441-020-03260-3
更新日期：2020-10-01 00:00:00
abstract：:An antiserum against conjugated histamine and two oligonucleotide probes that detect the mRNA encoding L-histidine decarboxylase (HDC) involved in histamine synthesis were used to study the appearance of histamine and its location in the kidneys of fetal, newborn and young postnatal rats and in the kidneys of pregnant...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00302960
更新日期：1992-02-01 00:00:00
abstract：:NCS-1 (neuronal calcium sensor) is a recently characterized member of a highly conserved neuron-specific family of calcium-binding proteins, which also includes frequenin and recoverin. The cellular and subcellular distributions of NCS-1 in the rat nervous system were investigated using light- and electron-microscopic...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410051246
更新日期：1999-03-01 00:00:00
abstract：:During the formation of dental enamel, maturation-stage ameloblasts express ion-transporting transmembrane proteins. The SLC4 family of ion-transporters regulates intra- and extracellular pH in eukaryotic cells by cotransporting HCO3 (-) with Na(+). Mutation in SLC4A4 (coding for the sodium-bicarbonate cotransporter N...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-014-1935-4
更新日期：2014-11-01 00:00:00
abstract：:Interstitial cells of Cajal (ICC) are important regulatory cells in the smooth muscle coats of the digestive tract. Expression of the Kit receptor tyrosine kinase was used in this study as a marker to study their distribution and development in the striated musculature of the mouse esophagus. Sections and whole-mounts...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s004410100418
更新日期：2001-10-01 00:00:00
abstract：:The spleen of the oriental fire-bellied toad, Bombina orientalis, consists of well-developed white pulp, separated from the lymphocytic marginal zone by the connective tissue boundary layer. Injection of peroxidase-conjugated rabbit anti-peroxidase revealed that these immune complexes were localized on the surface of ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00297553
更新日期：1993-01-01 00:00:00
abstract：:High-density cultures are widely used as an in vitro model for studies of embryonic cartilage formation. In the present study we investigated the suitability of high-density cultures for the redifferentiation of dedifferentiated chondrocytes. When primary human chondrocytes were cultured in alginate beads, some cells ...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-002-0562-7
更新日期：2002-06-01 00:00:00
abstract：:Coincident with an increase in the water permeability of toad urinary bladder induced by serosal hypertonicity, a transformation of the ridge-like surface structures of the granular cells into individual microvillous structures occurs. This study was initiated to establish whether the transformation is mediated by the...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00217226
更新日期：1984-01-01 00:00:00
abstract：:The H-Tx rat has fetal-onset hydrocephalus associated with closure of the cerebral aqueduct and a reduction in the secretory cells of the subcommissural organ (SCO), a circumventricular organ situated in the dorsal wall of the cerebral aqueduct. The objective of this study was to determine the role of the SCO in hydro...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/s00441-003-0843-9
更新日期：2004-03-01 00:00:00
abstract：:Gill lamellae of a bivalve Anodonta woodiana lauta (v. Martens) were observed by electron microscopy. The Anodonta gill wall consists of a single layer of epithelial cells, its basal lamina and the underlying connective tissue layer. It was confirmed that there is no true endothelium in the vessels and that the connec...
journal_title：Cell and tissue research
pub_type： 杂志文章
doi：10.1007/BF00222069
更新日期：1975-01-01 00:00:00
