Abstract:
:To enable stratification and barrier function, the epidermis must permit self-renewal while maintaining adhesive connections. By generating K14-GFP-actin mice to monitor actin dynamics in cultured primary keratinocytes, we uncovered a role for the actin cytoskeleton in establishing cellular organization. During epidermal sheet formation, a polarized network of nascent intercellular junctions and radial actin cables assemble in the apical plane of the monolayer. These actin fibers anchor to a central actin-myosin network, creating a tension-based plane of cytoskeleton across the apical surface of the sheet. Movement of the sheet surface relative to its base expands the zone of intercellular overlap, catalyzing new sites for nascent intercellular junctions. This polarized cytoskeleton is dependent upon alpha-catenin, Rho, and Rock, and its regulation may be important for wound healing and/or stratification, where coordinated tissue movements are involved.
journal_name
Dev Celljournal_title
Developmental cellauthors
Vaezi A,Bauer C,Vasioukhin V,Fuchs Edoi
10.1016/s1534-5807(02)00259-9keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
367-81issue
3eissn
1534-5807issn
1878-1551pii
S1534-5807(02)00259-9journal_volume
3pub_type
杂志文章abstract::Neutrophils, in response to a chemoattractant gradient, undergo dynamic F-actin remodeling, a process important for their directional migration or chemotaxis. However, signaling mechanisms for chemoattractants to regulate the process are incompletely understood. Here, we characterized chemoattractant-activated signali...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2011.10.023
更新日期:2011-12-13 00:00:00
abstract::Because mitochondria have outer and inner membranes, the fusion of two mitochondria requires the coordinated fusion of four lipid bilayers. Fusion of the outer membranes requires mitofusins. A new study published in Cell (Meeusen et al., 2006) shows that inner membrane fusion requires the dynamin-related protein Mgm1/...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2006.10.009
更新日期:2006-11-01 00:00:00
abstract::Fission yeast Pat1 kinase inhibits sexual differentiation by phosphorylating the meiotic inducer Mei2 and the transcription factor Ste11. Here, we show how Pat1 downregulates these proteins. Mei2 is degraded via a ubiquitin-proteasome pathway in a phosphorylation-dependent fashion. The E2 Ubc2 and the E3 Ubr1 are requ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(01)00037-5
更新日期:2001-09-01 00:00:00
abstract::Messenger RNAs (mRNAs) are synthesized in the cell nucleus and transported through nuclear pores to the cytoplasm for protein synthesis. Reporting in Nature Cell Biology, Mor et al. now track in living cells in real time the journey of single mRNA molecules as they transit from nucleus to cytoplasm. ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2010.05.021
更新日期:2010-06-15 00:00:00
abstract::Phase separation represents an important form of subcellular compartmentalization. However, relatively little is known about how the formation or disassembly of such compartments is regulated. In zebrafish, the Balbiani body (Bb) and the germ plasm (Gp) are intimately linked phase-separated structures essential for ge...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2018.07.009
更新日期:2018-08-06 00:00:00
abstract::We present the Real-time Accurate Cell-shape Extractor (RACE), a high-throughput image analysis framework for automated three-dimensional cell segmentation in large-scale images. RACE is 55-330 times faster and 2-5 times more accurate than state-of-the-art methods. We demonstrate the generality of RACE by extracting c...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2015.12.028
更新日期:2016-01-25 00:00:00
abstract::One of the hardest working mitotic proteins, Polo-like kinase 1 (PLK1), functions at mitotic entry, cytokinesis, and many steps in between. In this issue, Xie et al. (2020) describe a centrosome-specific interaction between PLK1 and Retinoid X Receptor-α and they test selective inhibition of this interaction as an ant...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2020.11.027
更新日期:2020-12-21 00:00:00
abstract::T-box genes encode DNA binding transcription factors known to regulate a wide variety of developmental processes during embryogenesis and are present in the genomes of all multicellular animals. Indeed, alongside other more familiar families of developmental regulators such as Hox, Sox, and Pax, T-box genes constitute...
journal_title:Developmental cell
pub_type:
doi:10.1016/s1534-5807(02)00330-1
更新日期:2002-11-01 00:00:00
abstract::Inherited mtDNA mutations cause severe human disease. In most species, mitochondria are inherited maternally through mechanisms that are poorly understood. Genes that specifically control the inheritance of mitochondria in the germline are unknown. Here, we show that the long isoform of the protein Oskar regulates the...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2016.11.004
更新日期:2016-12-05 00:00:00
abstract::Changes in the genetic makeup of an organism can extend lifespan significantly if they promote tolerance to environmental insults and thus prevent the general deterioration of cellular function that is associated with aging. Here, we introduce the Jun N-terminal kinase (JNK) signaling pathway as a genetic determinant ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(03)00323-x
更新日期:2003-11-01 00:00:00
abstract::Normal cellular development and function require tight spatiotemporal control of actin assembly. Formins are potent actin assembly factors that protect the growing ends of actin filaments from capping proteins. However, it is unresolved how the duration of formin-mediated actin assembly events is controlled, whether f...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2008.12.001
更新日期:2009-02-01 00:00:00
abstract::The genetic networks that govern vertebrate development are well studied, but how the interactions of trans-acting factors with cis-regulatory modules (CRMs) are integrated into spatiotemporal regulation of gene expression is not clear. The transcriptional regulator HAND2 is required during limb, heart, and branchial ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2014.09.018
更新日期:2014-11-10 00:00:00
abstract::Myelin is essential in vertebrates for the rapid propagation of action potentials, but the molecular mechanisms driving its formation remain largely unknown. Here we show that the initial stage of process extension and axon ensheathment by oligodendrocytes requires dynamic actin filament assembly by the Arp2/3 complex...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2015.06.011
更新日期:2015-07-27 00:00:00
abstract::Tet enzymes (Tet1/2/3) convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) in various embryonic and adult tissues. Mice mutant for either Tet1 or Tet2 are viable, raising the question of whether these enzymes have overlapping roles in development. Here we have generated Tet1 and Tet2 double-knockout (DKO)...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2012.12.015
更新日期:2013-02-11 00:00:00
abstract::The Par polarity complex creates mutually exclusive cortical domains in diverse animal cells. Activity of the atypical protein kinase C (aPKC) is a key output of the Par complex as phosphorylation removes substrates from the Par domain. Here, we investigate how diverse, apparently unrelated Par substrates couple phosp...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2015.09.016
更新日期:2015-10-26 00:00:00
abstract::Many human genes generate mRNAs with alternative 3' untranslated regions (3' UTRs), which can modulate protein function. In a recent Cell paper, Ma and Mayr (2018) unveil a major membraneless organelle, termed the TIS granule-endoplasmic reticulum (TIGER), which enables specific 3' UTRs to encipher protein localizatio...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2018.11.022
更新日期:2018-12-03 00:00:00
abstract::Nuclear size plays pivotal roles in gene expression, embryo development, and disease. A central hypothesis in organisms ranging from yeast to vertebrates is that nuclear size scales to cell size. This implies that nuclei may reach steady-state sizes set by limiting cytoplasmic pools of size-regulating components. By m...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2020.05.003
更新日期:2020-08-10 00:00:00
abstract::A remarkable feature of development is its reproducibility, the ability to correct embryo-to-embryo variations and instruct precise patterning. In Drosophila, embryonic patterning along the anterior-posterior axis is controlled by the morphogen gradient Bicoid (Bcd). In this article, we describe quantitative studies o...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2008.09.004
更新日期:2008-10-01 00:00:00
abstract::The crystal structure of a Wnt morphogen bound to its Frizzled receptor ectodomain provides insights into the evolutionary provenance of this complex fold and offers an explanation for why Wnts utilize both lipid- and protein-mediated contacts to engage Frizzleds. ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2012.07.011
更新日期:2012-08-14 00:00:00
abstract::Somatic muscle formation in Drosophila requires fusion of muscle founder cells with fusion-competent myoblasts. In a genetic screen for genes that control muscle development, we identified antisocial (ants), a gene that encodes an ankyrin repeat-, TPR repeat-, and RING finger-containing protein, required for myoblast ...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/s1534-5807(01)00084-3
更新日期:2001-11-01 00:00:00
abstract::Centrosomes in mammalian cells have recently been implicated in cytokinesis; however, their role in this process is poorly defined. Here, we describe a human coiled-coil protein, Cep55 (centrosome protein 55 kDa), that localizes to the mother centriole during interphase. Despite its association with gamma-TuRC anchori...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2005.09.003
更新日期:2005-10-01 00:00:00
abstract::Although many features of neurogenesis during development and in the adult are intrinsic to the neurogenic cells themselves, the role of the microenvironment is irrefutable. The neurogenic niche is a melting pot of cells and factors that influence CNS development. How do the diverse elements assemble and when? How doe...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2015.01.010
更新日期:2015-02-23 00:00:00
abstract::When two colonies of the tunicate Botryllus contact each other, they either fuse into a single colony or mount a destructive reaction, which keeps them apart. Which of the two reactions takes place is determined by a single, highly polymorphic fusion/histocompatibility (FuHC) locus. Recent communications report the cl...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2005.12.009
更新日期:2006-01-01 00:00:00
abstract::Cells in a variety of developmental contexts sense extracellular cues that are given locally on their surfaces, and subsequently amplify the initial signal to achieve cell polarization. Drosophila wing cells acquire planar polarity along the proximal-distal (P-D) axis, in which the amplification of the presumptive cue...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2005.11.016
更新日期:2006-02-01 00:00:00
abstract::Microtubules are non-covalent mesoscale polymers central to the eukaryotic cytoskeleton. Microtubule structure, dynamics, and mechanics are modulated by a cell's choice of tubulin isoforms and post-translational modifications, a "tubulin code," which is thought to support the diverse morphology and dynamics of microtu...
journal_title:Developmental cell
pub_type: 杂志文章,评审
doi:10.1016/j.devcel.2020.06.008
更新日期:2020-07-06 00:00:00
abstract::Recently reporting in Molecular Cell, Hussey et al. (2011) find that hnRNP E1 inhibits the translation of several genes essential for the epithelial-to-mesenchymal transition (EMT) by blocking eEF1A1 release during translation elongation. Phosphorylation of hnRNP E1 in response to TGF-β signaling disrupts the hnRNP E1...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2011.02.012
更新日期:2011-03-15 00:00:00
abstract::In this issue of Developmental Cell, Tang et al. (2018) and Li et al. (2018) combine genetic manipulation, mechanical perturbation, and live imaging to show how mechanical forces and local growth factors intersect to influence epithelial behavior and cell fate specification within the developing lung. ...
journal_title:Developmental cell
pub_type: 评论,杂志文章
doi:10.1016/j.devcel.2018.01.015
更新日期:2018-02-05 00:00:00
abstract::MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deletin...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2014.11.029
更新日期:2014-12-22 00:00:00
abstract::Centrosomes are important cell organizers. They consist of a pair of centrioles surrounded by pericentriolar material (PCM) that expands dramatically during mitosis-a process termed centrosome maturation. How centrosomes mature remains mysterious. Here, we identify a domain in Drosophila Cnn that appears to be phospho...
journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2014.02.013
更新日期:2014-03-31 00:00:00
abstract::Lipid droplets are fat storage organelles in cells that physically resemble stable oil-water emulsion droplets. In this issue of Developmental Cell, Ben M'barek et al. (2017) show that the resemblance is more than superficial: physical principles governing emulsion stability also control lipid droplet nucleation and g...
journal_title:Developmental cell
pub_type: 评论,杂志文章,评审
doi:10.1016/j.devcel.2017.06.001
更新日期:2017-06-19 00:00:00