Abstract：
:Normal cellular development and function require tight spatiotemporal control of actin assembly. Formins are potent actin assembly factors that protect the growing ends of actin filaments from capping proteins. However, it is unresolved how the duration of formin-mediated actin assembly events is controlled, whether formins are actively displaced from growing ends, and how filament length is regulated in vivo. Here, we identify Bud14 as a high-affinity inhibitor of the yeast formin Bnr1 that rapidly displaces the Bnr1 FH2 domain from growing barbed ends. Consistent with these activities, bud14Delta cells display fewer actin cables, which are aberrantly long, bent, and latrunculinA resistant, leading to defects in secretory vesicle movement. Moreover, bud14Delta suppressed mutations that cause abnormally numerous and shortened cables, restoring wild-type actin architecture. From these results, we propose that formin displacement factors regulate filament length and are required in vivo to maintain proper actin network architecture and function.
journal_name
Dev Celljournal_title
Developmental cellauthors
Chesarone M,Gould CJ,Moseley JB,Goode BLdoi
10.1016/j.devcel.2008.12.001subject
Has Abstractpub_date
2009-02-01 00:00:00pages
292-302issue
2eissn
1534-5807issn
1878-1551pii
S1534-5807(08)00511-Xjournal_volume
16pub_type
杂志文章abstract：:The viral genomes of alpha- and gamma-retroviruses follow an outbound route through the cytoplasm before assembling with the budding particle at the plasma membrane. We show here that murine leukemia virus (MLV) RNAs are transported on lysosomes and transferrin-positive endosomes. Transport on transferrin-positive ves...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/s1534-5807(03)00188-6
更新日期：2003-07-01 00:00:00
abstract：:Rab GTPases define the vesicle trafficking pathways underpinning cell polarization and migration. Here, we find that Rab4, Rab11, and Rab14 and the candidate Rab GDP-GTP exchange factors (GEFs) FAM116A and AVL9 are required for cell migration. Rab14 and its GEF FAM116A localize to and act on an intermediate compartmen...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2012.04.010
更新日期：2012-05-15 00:00:00
abstract：:Tight control of the number and distribution of crossovers is of great importance for meiosis. Crossovers establish chiasmata, which are physical connections between homologous chromosomes that provide the tension necessary to align chromosomes on the meiotic spindle. Understanding the mechanisms underlying crossover ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2008.07.006
更新日期：2008-09-01 00:00:00
abstract：:Polarity is a prominent feature of both chemotaxis and cytokinesis. In chemotaxis, polarity is established by local accumulation of PI(3,4,5)P3 at the cell's leading edge, achieved through temporal and spatial regulation of PI3 kinases and the tumor suppressor, PTEN. We find that as migrating D. discoideum cells round...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2005.02.010
更新日期：2005-04-01 00:00:00
abstract：:Angiogenesis is responsible for tissue vascularization during development, as well as in pathological contexts, including cancer and ischemia. Vascular endothelial growth factors (VEGFs) regulate angiogenesis by acting through VEGF receptors to induce endothelial cell signaling. VEGF is processed in the extracellular ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2016.12.017
更新日期：2017-01-23 00:00:00
abstract：:The emergence of limb-driven locomotor behaviors was a key event in the evolution of vertebrates and fostered the transition from aquatic to terrestrial life. We show that the generation of limb-projecting lateral motor column (LMC) neurons in mice relies on a transcriptional autoregulatory module initiated via transi...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2014.03.008
更新日期：2014-04-28 00:00:00
abstract：:Plasma membrane subdomains enriched in sphingolipids, cholesterol, and signaling proteins are critical for organization of actin, membrane trafficking, and cell polarity, but the role of such domains in cytokinesis in animal cells is unknown. Here, we show that eggs form a plasma membrane domain enriched in gangliosid...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2005.11.002
更新日期：2005-12-01 00:00:00
abstract：:Several genes have been implicated in heart tube formation, yet we know little about underlying cellular mechanisms. We analyzed the cellular architecture of the migrating myocardial precursors, and find that they form coherent epithelia that mature as they move medially. Mutant analyses indicate that the cardia bifid...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/s1534-5807(04)00063-2
更新日期：2004-03-01 00:00:00
abstract：:Major histocompatibility complex class Ib molecules may play a surprising role in pheromone detection in mammals. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章,评审
doi：10.1016/s1534-5807(03)00066-2
更新日期：2003-03-01 00:00:00
abstract：:Fibro/adipogenic progenitors (FAPs) are emerging as crucial regulators of fibrous and fat deposits during skeletal muscle regeneration. In a recent issue of Cell, Kopinke et al. (2017) report that primary cilia induce the adipogenic fate of FAPs in injured and diseased muscle by restraining Hedgehog signaling. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2017.06.023
更新日期：2017-07-24 00:00:00
abstract：:One G protein-coupled receptor (GPCR) can activate more than one G protein, but the physiologic importance of such activation has not been demonstrated in vivo. We have generated mice expressing exclusively a mutant form of the PTH/PTHrP receptor (DSEL) that activates adenylyl cyclase normally but not phospholipase C ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/s1534-5807(02)00218-6
更新日期：2002-08-01 00:00:00
abstract：:Recent studies on aging in model systems such as yeast and roundworms have revealed conserved regulation of the process in response to nutrient availability and specific genes that appear to mediate this regulation. Here we review these findings with a focus on the nematode Caenorhabditis elegans and the budding yeast...
journal_title：Developmental cell
pub_type： 杂志文章,评审
doi：10.1016/s1534-5807(01)00098-3
更新日期：2002-01-01 00:00:00
abstract：:In this issue of Developmental Cell, Jossin et al. (2017) show that the tumor suppressor protein Lgl1 interacts with N-cadherin to stabilize apical junctions in brain stem cells. Neural progenitors lacking Lgl1 had decreased N-cadherin internalization and abnormal cell junctions, generating an ectopic neuronal layer t...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2017.05.017
更新日期：2017-06-05 00:00:00
abstract：:Cell stress often triggers inflammation as a protective response to tissue damage or infection. In this issue of Developmental Cell, Sullivan et al. (2020) reveal an unexpected role for TRAIL receptors in ER-stress-induced inflammation. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2020.03.004
更新日期：2020-03-23 00:00:00
abstract：:While autophagy is thought to be an essential process in some cancer cells, it is unknown if or how such cancer cells can circumvent autophagy inhibition. To address this, we developed a CRISPR/Cas9 assay with dynamic live-cell imaging to measure acute effects of knockout (KO) of autophagy genes compared to known esse...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2019.07.010
更新日期：2019-09-23 00:00:00
abstract：:In a recent issue of Molecular Cell, Cairo et al. (2013) report that the spindle checkpoint protein Mad1 shuttles between unattached kinetochores and nuclear pores. Mobile Mad1 inhibits the import receptor Kap121p, thus indirectly changing spindle dynamics. This adds nuclear transport control to the mitotic roles of M...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2013.01.003
更新日期：2013-01-28 00:00:00
abstract：:The spindle assembly checkpoint ensures that mitotic cells only segregate their sister chromatids once all chromosomes are attached via kinetochores by microtubules of the mitotic spindle. Reporting in Developmental Cell, Silió et al. (2015) show that in human cells the signaling cascade controlling the checkpoint ope...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2015.11.021
更新日期：2015-12-07 00:00:00
abstract：:Although it is well appreciated that autophagy begins with phagophore formation and expansion through lipid acquisition to become the autophagosome, this process remains poorly understood, with the source of autophagosome membrane controversial. Reporting recently in Nature, Hamasaki et al. (2013) suggest that the ER-...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2013.04.004
更新日期：2013-04-29 00:00:00
abstract：:In this issue of Developmental Cell, Yao et al. (2019) disrupt mitochondrial respiration in limb skeletal progenitors by conditionally deleting Tfam in the mouse embryo. They discover that not only is mitochondrial respiration dispensable for embryonic cartilage development, but also its elimination obviates the need ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2019.05.030
更新日期：2019-06-03 00:00:00
abstract：:During embryonic development, cells must establish fates, morphologies, and behaviors in coordination with one another to form a functional body. A prevalent hypothesis for how this coordination is achieved is that each cell's fate and behavior is determined by a defined mixture of RNAs. Only recently has it become po...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2016.07.025
更新日期：2016-08-22 00:00:00
abstract：:To ensure equal chromosome segregation during mitosis, the macromolecular kinetochore must remain attached to depolymerizing microtubules, which drive chromosome movements. How kinetochores associate with depolymerizing microtubules, which undergo dramatic structural changes forming curved protofilaments, has yet to b...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2012.09.012
更新日期：2012-11-13 00:00:00
abstract：:In Drosophila, commitment of a cell to a sense organ precursor (SOP) fate requires bHLH proneural transcription factor upregulation, a process that depends in most cases on the interplay of proneural gene autoregulation and inhibitory Notch signaling. A subset of SOPs are selected by a recruitment pathway involving EG...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2004.09.015
更新日期：2004-11-01 00:00:00
abstract：:Tetrapod limbs display a great variety of forms and functions. In an attempt to understand factors influencing this diversity, Cretekos and colleagues, in a recent issue of Genes & Development, provide molecular insight into the evolution of wing morphology in bats. ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2008.01.011
更新日期：2008-02-01 00:00:00
abstract：:Centromeres form the site of chromosome attachment to microtubules during mitosis. Identity of these loci is maintained epigenetically by nucleosomes containing the histone H3 variant CENP-A. Propagation of CENP-A chromatin is uncoupled from DNA replication initiating only during mitotic exit. We now demonstrate that ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2011.10.014
更新日期：2012-01-17 00:00:00
abstract：:The extensive subcellular network of membrane contact sites plays central roles in organelle biogenesis and communication, yet the precise contributions of the involved machineries remain largely enigmatic. The yeast vacuole forms a membrane contact site with mitochondria, called vacuolar and mitochondrial patch (vCLA...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2018.05.011
更新日期：2018-06-04 00:00:00
abstract：:The 6th European Meiosis Workshop, sponsored by EMBO and organized by Franz Klein of the University of Vienna, met in mid-September. More than 150 participants who have not discovered anything more interesting than sex gathered in a bucolic Alpine valley near the town of Obertraun, Austria, surrounded by granite massi...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/s1534-5807(03)00336-8
更新日期：2003-11-01 00:00:00
abstract：:Steroid hormones have long been thought to enter target cells via passive diffusion through the plasma membrane. Now, reporting in Developmental Cell, Okamoto et al. (2018) demonstrate that, at least for Drosophila, steroid hormones require a protein transporter for cellular entry. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2018.10.022
更新日期：2018-11-05 00:00:00
abstract：:In this issue of Developmental Cell, Tang et al. (2018) and Li et al. (2018) combine genetic manipulation, mechanical perturbation, and live imaging to show how mechanical forces and local growth factors intersect to influence epithelial behavior and cell fate specification within the developing lung. ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2018.01.015
更新日期：2018-02-05 00:00:00
abstract：:Preventing inappropriate gene expression in time and space is as fundamental as triggering the activation of tissue- or cell-type-specific factors at the correct developmental stage and in the correct cells. Here, we study the impact of Polycomb repressive complex 2 (PRC2) function on HoxA gene regulation. We analyze ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2019.05.021
更新日期：2019-07-22 00:00:00
abstract：:Determination of left-right asymmetry in mouse embryos is established by a leftward fluid flow that is generated by clockwise rotation of node cilia. How node cilia achieve stable unidirectional rotation has remained unknown, however. Here we show that brief exposure to the microtubule-stabilizing drug paclitaxel (Tax...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2015.10.001
更新日期：2015-10-26 00:00:00
