Abstract:
:Rac signalling to actin -- a pathway that is thought to be mediated by the protein Scar/WAVE (WASP (Wiskott-Aldrich syndrome protein)-family verprolin homologous protein -- has a principal role in cell motility. In an analogous pathway, direct interaction of Cdc42 with the related protein N-WASP stimulates actin polymerization. For the Rac-WAVE pathway, no such direct interaction has been identified. Here we report a mechanism by which Rac and the adapter protein Nck activate actin nucleation through WAVE1. WAVE1 exists in a heterotetrameric complex that includes orthologues of human PIR121 (p53-inducible messenger RNA with a relative molecular mass (M(r)) of 140,000), Nap125 (NCK-associated protein with an M(r) of 125,000) and HSPC300. Whereas recombinant WAVE1 is constitutively active, the WAVE1 complex is inactive. We therefore propose that Rac1 and Nck cause dissociation of the WAVE1 complex, which releases active WAVE1-HSPC300 and leads to actin nucleation.
journal_name
Naturejournal_title
Natureauthors
Eden S,Rohatgi R,Podtelejnikov AV,Mann M,Kirschner MWdoi
10.1038/nature00859keywords:
subject
Has Abstractpub_date
2002-08-15 00:00:00pages
790-3issue
6899eissn
0028-0836issn
1476-4687pii
nature00859journal_volume
418pub_type
杂志文章相关文献
NATURE文献大全abstract::Many homeobox genes are clustered in a linear array along a chromosome, reflecting their ordered expression along the anterior-posterior axis of the embryo. Expression patterns as well as grafting, ectopic expression and loss-of-function experiments suggest that the Hox genes encode a combinatorial system of positiona...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/368747a0
更新日期:1994-04-21 00:00:00
abstract::Ribosomes are translational machineries that catalyse protein synthesis. Ribosome structures from various species are known at the atomic level, but obtaining the structure of the human ribosome has remained a challenge; efforts to address this would be highly relevant with regard to human diseases. Here we report the...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature14427
更新日期:2015-04-30 00:00:00
abstract::For parallel computers to simulate our brains, we must face the fact that human beings have a better claim on the title Homo seriatim than Homo sapiens--we're more consistently serial than wise. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/330033a0
更新日期:1987-11-05 00:00:00
abstract::The tumour suppressor p53 is required to induce programmed cell death (apoptosis) by DNA-damaging agents. As p53 is a transcriptional activator that mediates gene induction after DNA damage, it has been proposed to be a genetic switch that activates apoptosis-mediator genes. Here we evaluate the role of p53 in DNA-dam...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/370220a0
更新日期:1994-07-21 00:00:00
abstract::The genes torso (tor) and torso-like (tsl) are two of the Drosophila maternal group genes implicated in a receptor tyrosine kinase signalling pathway that specifies terminal cell fate (reviewed in ref. 3). Loss-of-function mutations in these loci cause an identical phenotype in which pattern elements from the anterior...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/367741a0
更新日期:1994-02-24 00:00:00
abstract::H-2 class I antigens appear to direct the recognition of virus-infected and neoplastic transformed cells by cytotoxic T lymphocytes (CTLs). Here, to identify the regions of class I antigens involved in CTL recognition, four hybrid class I genes were constructed in which exons were exchanged between the H-2Kb and H-2Db...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/309279a0
更新日期:1984-05-17 00:00:00
abstract::7SL RNA is an abundant cytoplasmic RNA which functions in protein secretion as a component of the signal recognition particle. Alu sequences are the most abundant family of human and rodent middle repetitive DNA sequences (reviewed in ref. 2). The primary structure of human 7SL RNA consists of an Alu sequence interrup...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/312171a0
更新日期:1984-11-08 00:00:00
abstract::Blood coagulation can be initiated when factor VII or VIIa, a plasma protease, binds to its essential cofactor, tissue factor (TF), and proteolytically activates factors IX and X, triggering a cascade of events which eventually leads to the formation of thrombin and a fibrin clot. Plasma contains a lipoprotein-associa...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/338518a0
更新日期:1989-04-06 00:00:00
abstract::In Drosophila and mice, olfactory receptor neurons (ORNs) expressing the same receptors have convergent axonal projections to specific glomerular targets in the antennal lobe/olfactory bulb, creating an odour map in this first olfactory structure of the central nervous system. Projection neurons of the Drosophila ante...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35102574
更新日期:2001-11-08 00:00:00
abstract::Humans differ from other primates in their significantly lengthened growth period. The persistence of a fetal pattern of brain growth after birth is another important feature of human development. Here we present the results of an analysis of the 1.8-million-year-old Mojokerto child (Perning 1, Java), the only well pr...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature02852
更新日期:2004-09-16 00:00:00
abstract::Haematopoietic stem cells (HSCs) have the ability to renew themselves and to give rise to all lineages of the blood; however, the signals that regulate HSC self-renewal remain unclear. Here we show that the Wnt signalling pathway has an important role in this process. Overexpression of activated beta-catenin expands t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01593
更新日期:2003-05-22 00:00:00
abstract::CDKN2A (INK4a/ARF) is frequently disrupted in various types of human cancer, and germline mutations of this locus can confer susceptibility to melanoma and other tumours. However, because CDKN2A encodes two distinct cell cycle inhibitory proteins, p16INK4a and p14ARF (p19Arf in mice), the mechanism of tumour suppressi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35092584
更新日期:2001-09-06 00:00:00
abstract::Mutations in phosphatase and tensin homologue (PTEN) or genomic alterations in the phosphatidylinositol-3-OH kinase-signalling pathway are the most common genetic alterations reported in human prostate cancer. However, the precise mechanism underlying how indolent tumours with PTEN alterations acquire metastatic poten...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11674
更新日期:2013-01-10 00:00:00
abstract::Activated T lymphocytes differentiate into effector cells tailored to meet disparate challenges to host integrity. For example, type 1 and type 2 helper (T(H)1 and T(H)2) cells secrete cytokines that enhance cell-mediated and humoral immunity, respectively. The chemokine monocyte chemoattractant protein-1 (MCP-1) can ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35006097
更新日期:2000-03-23 00:00:00
abstract::The increasing ability to control light-matter interactions at the nanometre scale has improved the performance of semiconductor lasers in the past decade. The ultimate optimization is realized in semiconductor microcavities, in which strong coupling between quantum-well excitons and cavity photons gives rise to hybri...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature06979
更新日期:2008-05-15 00:00:00
abstract::The neutron superfluid in most neutron stars should be in a highly turbulent state. If so, this turbulence drastically alters its rotational properties. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/227791a0
更新日期:1970-08-22 00:00:00
abstract::Recurring outbursts associated with matter flowing onto compact stellar remnants (such as black holes, neutron stars and white dwarfs) in close binary systems provide a way of constraining the poorly understood accretion process. The light curves of these outbursts are shaped by the efficiency of angular-momentum (and...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature25159
更新日期:2018-02-01 00:00:00
abstract::An optimal search theory, the so-called Lévy-flight foraging hypothesis, predicts that predators should adopt search strategies known as Lévy flights where prey is sparse and distributed unpredictably, but that Brownian movement is sufficiently efficient for locating abundant prey. Empirical studies have generated con...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09116
更新日期:2010-06-24 00:00:00
abstract::Methane is an important greenhouse gas that is emitted from multiple natural and anthropogenic sources. Atmospheric methane concentrations have varied on a number of timescales in the past, but what has caused these variations is not always well understood. The different sources and sinks of methane have specific isot...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature11461
更新日期:2012-10-04 00:00:00
abstract::Fossil dinosaur embryos are surprisingly rare, being almost entirely restricted to Upper Cretaceous strata that record the late stages of non-avian dinosaur evolution. Notable exceptions are the oldest known embryos from the Early Jurassic South African sauropodomorph Massospondylus and Late Jurassic embryos of a ther...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11978
更新日期:2013-04-11 00:00:00
abstract::Comprehensive protein protein interaction maps promise to reveal many aspects of the complex regulatory network underlying cellular function. Recently, large-scale approaches have predicted many new protein interactions in yeast. To measure their accuracy and potential as well as to identify biases, strengths and weak...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature750
更新日期:2002-05-23 00:00:00
abstract::Molecular approaches to understanding the functional circuitry of the nervous system promise new insights into the relationship between genes, brain and behaviour. The cellular diversity of the brain necessitates a cellular resolution approach towards understanding the functional genomics of the nervous system. We des...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature05453
更新日期:2007-01-11 00:00:00
abstract::The mec-2 gene is required for the function of a set of six touch receptor neurons in the nematode Caenorhabditis elegans; mec-2 mutants, which are touch-insensitive, have touch cells that appear morphologically normal. Gene interaction studies suggest that mec-2 positively regulates the activity of the putative mecha...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/378292a0
更新日期:1995-11-16 00:00:00
abstract::The rarity and inaccessibility of the earliest primordial germ cells (PGCs) in the mouse embryo thwart efforts to investigate molecular mechanisms of germ-cell specification. stella (also called Dppa3) marks the rare founder population of the germ lineage. Here we differentiate mouse embryonic stem cells carrying a st...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature08210
更新日期:2009-08-13 00:00:00
abstract::Lipoprotein(a) is an LDL-like lipoprotein whose concentration in plasma is correlated with atherosclerosis. The characteristic protein component of lipoprotein(a) is apolipoprotein(a) which is disulphide-linked to apolipoprotein B-100. Sequencing of cloned human apolipoprotein(a) complementary DNA shows that it is ver...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/330132a0
更新日期:1987-11-12 00:00:00
abstract::Double-stranded RNAs (dsRNAs) induce post-transcriptional gene silencing in several species of animal and plant. In plants, dsRNAs targeted to CpG islands within a promoter can also induce RNA-directed DNA methylation; however, it remains unclear whether gene silencing mediated by DNA methylation can be induced by dsR...
journal_title:Nature
pub_type: 杂志文章,收录出版
doi:10.1038/nature02889
更新日期:2004-09-09 00:00:00
abstract::The peptide-loading complex (PLC) is a transient, multisubunit membrane complex in the endoplasmic reticulum that is essential for establishing a hierarchical immune response. The PLC coordinates peptide translocation into the endoplasmic reticulum with loading and editing of major histocompatibility complex class I (...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature24627
更新日期:2017-11-23 00:00:00
abstract::The identification of properties that contribute to the persistence and resilience of ecosystems despite climate change constitutes a research priority of global relevance. Here we present a novel, empirical approach to assess the relative sensitivity of ecosystems to climate variability, one property of resilience th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature16986
更新日期:2016-03-10 00:00:00
abstract::The clinical benefit for patients with diverse types of metastatic cancers that has been observed upon blockade of the interaction between PD-1 and PD-L1 has highlighted the importance of this inhibitory axis in the suppression of tumour-specific T-cell responses. Notwithstanding the key role of PD-L1 expression by ce...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature23669
更新日期:2017-09-07 00:00:00
abstract::Mitogen-activated protein kinases, MAP kinases or ERKs (extracellular signal-regulated kinases) are rapidly stimulated by growth-promoting factors acting on a variety of cell-surface receptors. In turn, ERKs phosphorylate and regulate key intracellular enzymes and transcription factors involved in the control of cellu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/369418a0
更新日期:1994-06-02 00:00:00