Abstract:
:The tumour suppressor p53 is required to induce programmed cell death (apoptosis) by DNA-damaging agents. As p53 is a transcriptional activator that mediates gene induction after DNA damage, it has been proposed to be a genetic switch that activates apoptosis-mediator genes. Here we evaluate the role of p53 in DNA-damage-induced apoptosis by establishing derivatives of GHFT1 cells, that are somatotropic progenitors immortalized by expression of SV40 T-antigen, which express a temperature-sensitive p53 mutant. In these cells induction of apoptosis by DNA damage depends strictly on p53 function. A shift to the permissive temperature triggers apoptosis following DNA damage, but this is independent of new RNA or protein synthesis. The extent of apoptotic DNA cleavage is directly proportional to the period during which p53 is functional. These results do not support the proposal that p53 is an activator of apoptosis-mediator genes but rather indicate that p53 either represses genes necessary for cell survival or is a component of the enzymatic machinery for apoptotic cleavage or repair of DNA.
journal_name
Naturejournal_title
Natureauthors
Caelles C,Helmberg A,Karin Mdoi
10.1038/370220a0subject
Has Abstractpub_date
1994-07-21 00:00:00pages
220-3issue
6486eissn
0028-0836issn
1476-4687journal_volume
370pub_type
杂志文章相关文献
NATURE文献大全abstract::Neuronal function depends crucially on the spatial segregation of specific membrane proteins, particularly the segregation associated with sites of synaptic contact. Understanding the factors governing this localization of proteins is a major goal of cellular neurobiology. A conspicuous example of synaptic specializat...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/313588a0
更新日期:1985-02-14 00:00:00
abstract::Nutrients, such as amino acids and glucose, signal through the Rag GTPases to activate mTORC1. The GATOR1 protein complex-comprising DEPDC5, NPRL2 and NPRL3-regulates the Rag GTPases as a GTPase-activating protein (GAP) for RAGA; loss of GATOR1 desensitizes mTORC1 signalling to nutrient starvation. GATOR1 components h...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature26158
更新日期:2018-04-05 00:00:00
abstract::The development of colloidal quantum dots has led to practical applications of quantum confinement, such as in solution-processed solar cells, lasers and as biological labels. Further scientific and technological advances should be achievable if these colloidal quantum systems could be electronically coupled in a gene...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02695
更新日期:2004-07-08 00:00:00
abstract::CD95 (also called Fas and APO-1) is a prototypical death receptor that regulates tissue homeostasis mainly in the immune system through the induction of apoptosis. During cancer progression CD95 is frequently downregulated or cells are rendered apoptosis resistant, raising the possibility that loss of CD95 is part of ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09075
更新日期:2010-05-27 00:00:00
abstract::All synaptically-based neuropsychological theories of learning postulate that there are changes resulting from neural activity which are long-lasting and confined to specific sets of synapses. In the past decade a form of synaptic strengthening known as long-term potentiation (LTP) has been found which results from hi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/305717a0
更新日期:1983-10-20 00:00:00
abstract::Oxygen consumption by carnivorous reptiles increases enormously after they have eaten a large meal in order to meet metabolic demands, and this places an extra load on the cardiovascular system. Here we show that there is an extraordinarily rapid 40% increase in ventricular muscle mass in Burmese pythons (Python molur...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/434037a
更新日期:2005-03-03 00:00:00
abstract::Electronic devices have advanced from their heavy, bulky origins to become smart, mobile appliances. Nevertheless, they remain rigid, which precludes their intimate integration into everyday life. Flexible, textile and stretchable electronics are emerging research areas and may yield mainstream technologies. Rollable ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12314
更新日期:2013-07-25 00:00:00
abstract::Calcium (Ca(2+))-activated chloride channels are fundamental mediators in numerous physiological processes including transepithelial secretion, cardiac and neuronal excitation, sensory transduction, smooth muscle contraction and fertilization. Despite their physiological importance, their molecular identity has remain...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07313
更新日期:2008-10-30 00:00:00
abstract::The intestinal tract maintains proper function by replacing aged cells with freshly produced cells that arise from a population of self-renewing intestinal stem cells (ISCs). In the mammalian intestine, ISC self renewal, amplification and differentiation take place along the crypt-villus axis, and are controlled by th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07156
更新日期:2008-07-31 00:00:00
abstract::How environmental cues regulate adult stem cell and cancer cell activity through surface receptors is poorly understood. Angiopoietin-like proteins (ANGPTLs), a family of seven secreted glycoproteins, are known to support the activity of haematopoietic stem cells (HSCs) in vitro and in vivo. ANGPTLs also have importan...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11095
更新日期:2012-05-30 00:00:00
abstract::During the past ten years, coupled quantum wells have emerged as a promising system for experiments on Bose condensation of excitons, with numerous theoretical and experimental studies aimed at the demonstration of this effect. One of the issues driving these studies is the possibility of long-range coherent transport...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature00940
更新日期:2002-08-15 00:00:00
abstract::Mass spectrometry is a powerful technology for the analysis of large numbers of endogenous proteins. However, the analytical challenges associated with comprehensive identification and relative quantification of cellular proteomes have so far appeared to be insurmountable. Here, using advances in computational proteom...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature07341
更新日期:2008-10-30 00:00:00
abstract::Fireflies communicate with each other by emitting yellow-green to yellow-orange brilliant light. The bioluminescence reaction, which uses luciferin, Mg-ATP and molecular oxygen to yield an electronically excited oxyluciferin species, is carried out by the enzyme luciferase. Visible light is emitted during relaxation o...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04542
更新日期:2006-03-16 00:00:00
abstract::Observations of galaxies and primordial radiation suggest that the Universe is made mostly of non-luminous dark matter1,2. Several new types of fundamental particle have been proposed as candidates for dark matter3, such as weakly interacting massive particles (WIMPs)4,5. These particles would be expected to interact ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-018-0739-1
更新日期:2018-12-01 00:00:00
abstract::Most of the progress in human culture has required the exploitation of energy resources. About 100 years ago, the major source of energy shifted from recent solar to fossil hydrocarbons, including liquid and gaseous petroleum. Technology has generally led to a greater use of hydrocarbon fuels for most human activities...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature02130
更新日期:2003-11-20 00:00:00
abstract::It is thought that in skeletal muscle excitation-contraction (EC) coupling, the release of Ca2+ from the sarcoplasmic reticulum is controlled by the dihydropyridine (DHP) receptor in the transverse tubular membrane, where it serves as the voltage sensor. We have shown previously that injection of an expression plasmid...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/346567a0
更新日期:1990-08-09 00:00:00
abstract:: ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/d41586-018-07501-y
更新日期:2018-11-01 00:00:00
abstract::Members of the Hedgehog family of secreted proteins control a number of important inductive interactions in the development of both vertebrates and Drosophila, but little is known about the ways in which their signalling activities are transduced. In Drosophila, hedgehog is one of the segment-polarity genes, mutations...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/382547a0
更新日期:1996-08-08 00:00:00
abstract::When chemical reactions whose rate increases with the concentration of a product species are carried out in imperfectly mixed systems, a variety of complex behaviours can occur. These phenomena, which have relevance for biological processes as well, include chaotic and stochastic behaviour and selection of one final s...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/374321a0
更新日期:1995-03-23 00:00:00
abstract::Magnetic fields play a part in almost all stages of stellar evolution. Most low-mass stars, including the Sun, show surface fields that are generated by dynamo processes in their convective envelopes. Intermediate-mass stars do not have deep convective envelopes, although 10 per cent exhibit strong surface fields that...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature16171
更新日期:2016-01-21 00:00:00
abstract::Joining dissimilar materials such as plastics and metals in engineered structures remains a challenge1. Mechanical fastening, conventional welding and adhesive bonding are examples of techniques currently used for this purpose, but each of these methods presents its own set of problems2 such as formation of stress con...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2813-8
更新日期:2020-10-01 00:00:00
abstract::The immunophilins cyclophilin and FK506 binding protein (FKBP) are small, predominantly soluble proteins that bind the immunosuppressant drugs cyclosporin A and FK506, respectively, with high affinity, and which seem to mediate their pharmacological actions. The Ca(2+)-dependent protein phosphatase, calcineurin, binds...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/358584a0
更新日期:1992-08-13 00:00:00
abstract::The distribution of geochemical species in the Earth's interior is largely controlled by fractional melting and crystallization processes that are intimately linked to the thermal state and evolution of the mantle. The existence of patches of dense partial melt at the base of the Earth's mantle, together with estimate...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature06355
更新日期:2007-12-06 00:00:00
abstract::The masses of the supermassive black holes found in galaxy bulges are correlated with a multitude of galaxy properties, leading to suggestions that galaxies and black holes may evolve together. The number of reliably measured black-hole masses is small, and the number of methods for measuring them is limited, holding ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11819
更新日期:2013-02-21 00:00:00
abstract::Magma intrusions and eruptions commonly produce abrupt changes in seismicity far from magma conduits that cannot be associated with the diffusion of pore fluids or heat. Such 'swarm' seismicity also migrates with time, and often exhibits a 'dog-bone'-shaped distribution. The largest earthquakes in swarms produce after...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature00997
更新日期:2002-09-05 00:00:00
abstract::The development of a eutherian mammal as a male is a consequence of testis formation in the embryo, which is thought to be initiated by a gene on the Y chromosome. In the absence of this gene, ovaries are formed and female characteristics develop. Sex determination therefore hinges on the action of this testis-determi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/348450a0
更新日期:1990-11-29 00:00:00
abstract::The crown-of-thorns starfish (COTS, the Acanthaster planci species group) is a highly fecund predator of reef-building corals throughout the Indo-Pacific region. COTS population outbreaks cause substantial loss of coral cover, diminishing the integrity and resilience of reef ecosystems. Here we sequenced genomes of CO...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature22033
更新日期:2017-04-05 00:00:00
abstract::Monoclonal antibodies can now be genetically engineered and endowed with new properties. In the future, gene technology could enable antigen-binding fragments to be made by exploiting repertoires of variable domain genes derived from immunized animals and expressed in bacteria. How readily can this approach be extende...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/349293a0
更新日期:1991-01-24 00:00:00
abstract::Some surprising facts about the most productive institutions emerge when Japanese life-science research is subject to a novel type of assessment. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/372125a0
更新日期:1994-11-10 00:00:00
abstract::The nucleotide sequence of the RNA polymerase binding site at a promotor on fd RF DNA has been determined in comparison with the starting sequence of RNA initiated at this promoter. The RNA polymerase binding site contained the startpoint of transcription in the centre and a region with twofold symmetry in the non-tra...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/253410a0
更新日期:1975-02-06 00:00:00