Abstract:
:We established the procedures for cryopreservation and primary culture of fetal cerebral neurons of cynomolgus monkeys (Macaca fascicularis). Three developmental stages of fetuses (80, 93, and 102 days of gestation) were compared to determine the optimal stage of cerebrum development for primary culture. Among the three fetuses, the 80-day-old fetus produced the most process-rich neurons with the highest survival. The number of total recovery cells from the cryopreserved 80-day-old fetus corresponded to 83.4% of that from fresh tissue. Besides, synchronous oscillations of intracellular calcium were first seen in primate cerebral neurons, which suggested the formation of synapse-networks. Cultured neurons expressed synaptophysin protein. Successful cryopreservation and subsequent cell culture of primate neurons would be useful tools for neuroscience research with species specificity.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Negishi T,Ishii Y,Kawamura S,Kuroda Y,Yoshikawa Ydoi
10.1016/s0304-3940(02)00433-0keywords:
subject
Has Abstractpub_date
2002-08-02 00:00:00pages
21-4issue
1eissn
0304-3940issn
1872-7972pii
S0304394002004330journal_volume
328pub_type
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
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journal_title:Neuroscience letters
pub_type: 杂志文章
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journal_title:Neuroscience letters
pub_type: 杂志文章
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更新日期:2002-12-19 00:00:00
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journal_title:Neuroscience letters
pub_type: 杂志文章
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