Abstract:
:The efficiency of antisense approaches to produce a selective regional inhibition of the expression of brain 5-HT1A receptors was tested in the rat. In vivo ICV injections of modified antisense oligodesoxynucleotides yielded at most an 18% specific decrease in 5-HT1A receptor expression in the hippocampus only, as measured by [3H]8-OH-DPAT autoradiographic labeling. In vitro, when 5-HT1A receptors were transiently expressed in LLC-PK1 cells, co-transfection with antisense RNA encoding plasmids resulted in a marked reduction (50-70%) in the density of 5-HT1A binding sites. In vivo stereotaxic injections of the same constructs into the hippocampus, but not in the raphe, which contains 5-HT1A autoreceptors, were shown to produce a approximately 20% reduction in local 5-HT1A receptor density. These data show that antisense strategies could be used to inhibit 5-HT1A receptors expression in the rat hippocampus, but with a limited efficacy.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Sari Y,Sibella C,Vergé D,Hamon M,Miquel MCdoi
10.1016/s0304-3940(98)00925-2keywords:
subject
Has Abstractpub_date
1999-01-15 00:00:00pages
191-5issue
3eissn
0304-3940issn
1872-7972pii
S0304394098009252journal_volume
259pub_type
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