Genome-based identification of chromosomal regions specific for Salmonella spp.

Abstract:

:Acquisition of genomic elements by horizontal gene transfer represents an important mechanism in the evolution of bacterial species. Pathogenicity islands are a subset of horizontally acquired elements present in various pathogens. These elements are frequently located adjacent to tRNA genes. We performed a comparative genome analysis of Salmonella enterica serovars Typhi and Typhimurium and Escherichia coli and scanned tRNA loci for the presence of species-specific, horizontally acquired genomic elements. A large number of species-specific elements were identified. Here, we describe the characteristics of four large chromosomal insertions at tRNA genes of Salmonella spp. The tRNA-associated elements harbor various genes previously identified as single virulence genes, indicating that these genes have been acquired with large chromosomal insertions. Southern blot analyses confirmed that the tRNA-associated elements are specific to Salmonella and also indicated a heterogeneous distribution within the salmonellae. Systematic scanning for insertions at tRNA genes thus represents a tool for the identification of novel pathogenicity islands.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Hansen-Wester I,Hensel M

doi

10.1128/iai.70.5.2351-2360.2002

keywords:

subject

Has Abstract

pub_date

2002-05-01 00:00:00

pages

2351-60

issue

5

eissn

0019-9567

issn

1098-5522

journal_volume

70

pub_type

杂志文章
  • Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum 775.

    abstract::Cells of the marine fish pathogen Vibrio anguillarum 775 harboring a plasmid associated with virulence can grow unaffected in the presence of iron-binding compounds such as transferrin. In contrast, the growth of isogenic plasmidless derivatives is inhibited by the presence of iron chelators. Radioactive from (55Fe3+)...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.31.1.223-227.1981

    authors: Crosa JH,Hodges LL

    更新日期:1981-01-01 00:00:00

  • Analysis of the lytic activity of the Serpulina hyodysenteriae hemolysin.

    abstract::The hemolysins of Serpulina hyodysenteriae are active at 27 to 40 degrees C and pH 3 to 9 and are unaffected by enzymatic inhibitors. Pore formation was demonstrated by the inhibition of hemolysis with molecules of 2.0 to 2.3 nm in diameter and the release of 86rubidium from erythrocytes without hemoglobin release aft...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.11.4877-4879.1997

    authors: Hyatt DR,Joens LA

    更新日期:1997-11-01 00:00:00

  • Release of cytokines induced by Salmonella typhimurium porins.

    abstract::Salmonella typhimurium SH5014 porins induce the release of tumor necrosis factor alpha (TNF-alpha), interleukin 1 alpha (IL-1 alpha), and IL-6 by human monocytes and of gamma interferon (IFN-gamma) and IL-4 by human lymphocytes. Porins at 1 microgram/ml induce the greatest release of TNF-alpha, IL-1 alpha, and IL-6 by...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.1.155-161.1993

    authors: Galdiero F,de L'ero GC,Benedetto N,Galdiero M,Tufano MA

    更新日期:1993-01-01 00:00:00

  • Effect on intraerythrocytic Anaplasma marginale of soluble factors from infected calf blood mononuclear cells.

    abstract::Blood mononuclear cells (lymphocytes and monocytes) were isolated from infected calves during in vivo control of acute anaplasmosis and cultured with Anaplasma marginale organisms. Supernatants from the cultures reduced the proportion of erythrocytes containing viable A. marginale in vitro, indicating that an antibody...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.11.4846-4849.1996

    authors: Wyatt CR,Davis WC,Knowles DP,Goff WL,Palmer GH,McGuire TC

    更新日期:1996-11-01 00:00:00

  • Reactive oxygen species-triggered trophoblast apoptosis is initiated by endoplasmic reticulum stress via activation of caspase-12, CHOP, and the JNK pathway in Toxoplasma gondii infection in mice.

    abstract::Toxoplasma gondii infection in pregnant women may result in abortion or in fetal teratogenesis; however, the underlying mechanisms are still unclear. In this paper, based on a murine model, we showed that maternal infection with RH strain T. gondii tachyzoites induced elevated production of reactive oxygen species (RO...

    journal_title:Infection and immunity

    pub_type: 杂志文章,收录出版

    doi:10.1128/IAI.06295-11

    authors: Xu X,Liu T,Zhang A,Huo X,Luo Q,Chen Z,Yu L,Li Q,Liu L,Lun ZR,Shen J

    更新日期:2012-06-01 00:00:00

  • Expression of cathelicidin LL-37 during Mycobacterium tuberculosis infection in human alveolar macrophages, monocytes, neutrophils, and epithelial cells.

    abstract::The innate immune response in human tuberculosis is not completely understood. To improve our knowledge regarding the role of cathelicidin hCAP-18/LL37 in the innate immune response to tuberculosis infection, we used immunohistochemistry, immunoelectron microscopy, and gene expression to study the induction and produc...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01218-07

    authors: Rivas-Santiago B,Hernandez-Pando R,Carranza C,Juarez E,Contreras JL,Aguilar-Leon D,Torres M,Sada E

    更新日期:2008-03-01 00:00:00

  • Ultrastructural study of adherence to and penetration of cultured cells by two invasive Escherichia coli strains isolated from infants with enteritis.

    abstract::The adherence of invasive Escherichia coli strains 444-3 and 469-3 to human erythrocytes and to cultured HeLa and HEp-2 cells has been examined by electron microscopy. Bacteria elaborating type 1 fimbriae, glycocalyces , and nonfimbrial mannose-resistant hemagglutinins specific for human erythrocytes were identified i...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.44.3.599-608.1984

    authors: Knutton S,Williams PH,Lloyd DR,Candy DC,McNeish AS

    更新日期:1984-06-01 00:00:00

  • Serological purification of polysaccharide antigens from Streptococcus mutans serotypes a and d: characterization of multiple antigenic determinants.

    abstract::The polysaccharide antigen preparations from serotype a and serotype d strains of Streptococcus mutans contained both a serotype-specific antigenic determinant and a common a-d antigenic determinant, as demonstrated by agar gel diffusion studies and a quantitative cross-precipitin assay. The chromatographically purifi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.12.4.791-798.1975

    authors: Linzer R,Mukasa H,Slade HD

    更新日期:1975-10-01 00:00:00

  • Volatile fatty acid, metabolic by-product of periodontopathic bacteria, induces apoptosis in WEHI 231 and RAJI B lymphoma cells and splenic B cells.

    abstract::The ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine WEHI 231 cells, splenic B cells, and human RAJI cells was examined. The culture filtrate of Porphyromonas gingivalis, Prevotella loescheii, and Fusobacterium nucleatum, which contains high a percentag...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.6.2587-2594.1998

    authors: Kurita-Ochiai T,Ochiai K,Fukushima K

    更新日期:1998-06-01 00:00:00

  • A Point Mutation in carR Is Involved in the Emergence of Polymyxin B-Sensitive Vibrio cholerae O1 El Tor Biotype by Influencing Gene Transcription.

    abstract::Antimicrobial peptides play an important role in host defense against Vibrio cholerae Generally, the V. cholerae O1 classical biotype is polymyxin B (PB) sensitive and El Tor is relatively resistant. Detection of classical biotype traits like the production of classical cholera toxin and PB sensitivity in El Tor strai...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00080-20

    authors: Samanta P,Mandal RS,Saha RN,Shaw S,Ghosh P,Dutta S,Ghosh A,Imamura D,Morita M,Ohnishi M,Ramamurthy T,Mukhopadhyay AK

    更新日期:2020-04-20 00:00:00

  • Participation of the Serine Protease Jonah66Ci in the Drosophila Antinematode Immune Response.

    abstract::Serine proteases and serine protease homologs form the second largest gene family in the Drosophila melanogaster genome. Certain genes in the Jonah multigene family encoding serine proteases have been implicated in the fly antiviral immune response. Here, we report the involvement of Jonah66Ci in the Drosophila immune...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00094-19

    authors: Yadav S,Eleftherianos I

    更新日期:2019-08-21 00:00:00

  • In vivo induction of interleukin-12 mRNA expression after oral immunization with Salmonella dublin or the B subunit of Escherichia coli heat-labile enterotoxin.

    abstract::Mice orally immunized with Salmonella dublin EL23, a nonreverting, aromatic-dependent, histidine-requiring mutant transformed with a plasmid which carries a gene that codes for production of the B subunit of the heat-labile toxin (LT-B) of enterotoxigenic Escherichia coli, or with purified LT-B alone were compared for...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.3.1076-1083.1995

    authors: Bost KL,Clements JD

    更新日期:1995-03-01 00:00:00

  • NK T cells are a source of early interleukin-4 following infection with third-stage larvae of the filarial nematode Brugia pahangi.

    abstract::Infection of C57BL/6 mice with the third-stage larvae of Brugia pahangi results in a rapid expansion of NK1.1(+) T cells in the spleen and draining lymph nodes. NK T cells produced interleukin-4 in the spleen within 24 h of infection, and these cells were CD4(-). ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.4.2215-2219.2002

    authors: Balmer P,Devaney E

    更新日期:2002-04-01 00:00:00

  • Role of capsule and suilysin in mucosal infection of complement-deficient mice with Streptococcus suis.

    abstract::Virulent Streptococcus suis serotype 2 strains are invasive extracellular bacteria causing septicemia and meningitis in piglets and humans. One objective of this study was to elucidate the function of complement in innate immune defense against S. suis. Experimental infection of wild-type (WT) and C3(-/-) mice demonst...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00080-14

    authors: Seitz M,Beineke A,Singpiel A,Willenborg J,Dutow P,Goethe R,Valentin-Weigand P,Klos A,Baums CG

    更新日期:2014-06-01 00:00:00

  • The psa locus is responsible for thermoinducible binding of Yersinia pseudotuberculosis to cultured cells.

    abstract::Yersinia pseudotuberculosis inv mutant strains cured of the virulence plasmid exhibit thermoinducible adhesion to cultured mammalian cells. To identify the genes responsible for this phenotype, Y. pseudotuberculosis homologs of the Y. enterocolitica ail and the Y. pestis psa loci were identified. Mutations in the Y. p...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.7.2483-2489.1996

    authors: Yang Y,Merriam JJ,Mueller JP,Isberg RR

    更新日期:1996-07-01 00:00:00

  • Interaction of Gardnerella vaginalis and Vaginolysin with the Apical versus Basolateral Face of a Three-Dimensional Model of Vaginal Epithelium.

    abstract::Studies have implicated Gardnerella vaginalis as an important etiological agent in bacterial vaginosis (BV). It produces a cholesterol-dependent cytolysin, vaginolysin (VLY). In this study, we sought to characterize the interaction between vaginal epithelium, G. vaginalis, and VLY using EpiVaginal tissues from MatTek....

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00646-18

    authors: Garcia EM,Kraskauskiene V,Koblinski JE,Jefferson KK

    更新日期:2019-03-25 00:00:00

  • The AraC family transcriptional regulator Rv1931c plays a role in the virulence of Mycobacterium tuberculosis.

    abstract::A Mycobacterium tuberculosis strain disrupted in the AraC homologue Rv1931c was isolated. The mutant strain exhibited reduced survival both in macrophages and in a mouse infection model, with survival being restored on complementation with the Rv1931c gene. These results suggest that Rv1931c regulates genes important ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.72.9.5483-5486.2004

    authors: Frota CC,Papavinasasundaram KG,Davis EO,Colston MJ

    更新日期:2004-09-01 00:00:00

  • Effects of zinc deficiency and pneumococcal surface protein a immunization on zinc status and the risk of severe infection in mice.

    abstract::Streptococcus pneumoniae is a major cause of illness and death in children in developing countries. In these children, zinc deficiency is associated with an increased risk of acute respiratory tract infections, which can be reduced by daily zinc administration. Severe infections decrease zinc levels in plasma and may ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.4.2009-2013.2003

    authors: Strand TA,Hollingshead SK,Julshamn K,Briles DE,Blomberg B,Sommerfelt H

    更新日期:2003-04-01 00:00:00

  • Deletion of wboA enhances activation of the lectin pathway of complement in Brucella abortus and Brucella melitensis.

    abstract::Brucella spp. are gram-negative intracellular pathogens that survive and multiply within phagocytic cells of their hosts. Smooth organisms present O polysaccharides (OPS) on their surface. These OPS help the bacteria avoid the bactericidal action of serum. The wboA gene, coding for the enzyme glycosyltransferase, is e...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.7.4407-4416.2001

    authors: Fernandez-Prada CM,Nikolich M,Vemulapalli R,Sriranganathan N,Boyle SM,Schurig GG,Hadfield TL,Hoover DL

    更新日期:2001-07-01 00:00:00

  • Two distinct cytotoxic activities of subtilase cytotoxin produced by shiga-toxigenic Escherichia coli.

    abstract::Subtilase cytotoxin (SubAB) is a recently identified AB5 subunit toxin produced by Shiga-toxigenic Escherichia coli. The A subunit is thought to be a subtilase-like, serine protease, whereas the B subunit binds to the toxin receptor on the cell surface. We cloned the genes from a clinical isolate; the toxin was produc...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01336-06

    authors: Morinaga N,Yahiro K,Matsuura G,Watanabe M,Nomura F,Moss J,Noda M

    更新日期:2007-01-01 00:00:00

  • Antigenic relatedness of glucosyltransferase enzymes from streptococcus mutans.

    abstract::The antigenic relationship of glucosyltransferases (GTF) produced by different serotypes of Streptococcus mutans was studied by using a functional inhibition assay. Rat, rabbit, or hamster immune fluids, directed to cell-associated or supernatant-derived GTF, were tested against ammonium sulfate-precipitated culture s...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.15.1.91-103.1977

    authors: Smith DJ,Taubman MA

    更新日期:1977-01-01 00:00:00

  • Reactivity profile of human anti-82-kilodalton rhoptry protein antibodies generated during natural infection with Plasmodium falciparum.

    abstract::Immunization of monkeys with the 82-kDa rhoptry protein (p82) of Plasmodium falciparum can protect them against a lethal blood stage challenge, and monoclonal antibodies to p82 inhibit parasite growth in vitro. The role that a p82-specific immune response might play in human immunity to the parasite is not known. To d...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.7.2960-2965.1993

    authors: Howard RF,Jensen JB,Franklin HL

    更新日期:1993-07-01 00:00:00

  • Galactomannan antigenemia in invasive aspergillosis.

    abstract::Galactomannan (GM) extracted from mycelia of Aspergillus fumigatus with cold dilute alkali reacted with antiserum specific for an antigen that circulated in invasive aspergillosis in rabbits and humans. The GM was purified by its affinity for concanavalin A and was separated from a nonantigenic glucan by gel permeatio...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.25.1.357-365.1979

    authors: Reiss E,Lehmann PF

    更新日期:1979-07-01 00:00:00

  • Campylobacter-Wolinella group organisms are the only oral bacteria that form arylsulfatase-active colonies on a synthetic indicator medium.

    abstract::Most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. Arylsulfatase activity was, however, restricted to Campylobacter-Wolinella group organisms, including Wolinella recta, a possible periodontopathogen. W. recta was the only arylsulfatase-active species a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.5.1380-1383.1989

    authors: Wyss C

    更新日期:1989-05-01 00:00:00

  • Tracheal function during influenza infections.

    abstract::Studies with animal models have demonstrated that viral respiratory tract infections suppress bacterial clearance processes in the lung and that this alteration in host defenses appears to explain the excessive mortality from bacterial pneumonia during influenza epidemics. However, since the pathogenesis of postinflue...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.42.3.1102-1108.1983

    authors: Nugent KM,Pesanti EL

    更新日期:1983-12-01 00:00:00

  • Passive hemolysis test for antibody to Treponema hyodysenteriae.

    abstract::Swine were infected orally with pure cultures of Treponema hyodysenteriae, and the humoral antibody response was measured by a passive hemolysis test (passive hemagglutination test with the use of complement). Antibody to T. hyodysenteriae was detected as early as 1 week and later on at 4 months after exposure. Howeve...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.14.4.1106-1108.1976

    authors: Jenkins EM,Sinha PP,Vance RT,Reese GL

    更新日期:1976-10-01 00:00:00

  • Quantification of the relative contribution of major histocompatibility complex (MHC) and non-MHC genes to human immune responses to foreign antigens.

    abstract::Understanding the extent to which genetic factors influence the immune response is important in the development of subunit vaccines. Associations with HLA gene polymorphisms appear insufficient to explain the range of variation in immune responses to vaccines and to infections by major pathogens. In this study of Gamb...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.3.872-876.1997

    authors: Jepson A,Banya W,Sisay-Joof F,Hassan-King M,Nunes C,Bennett S,Whittle H

    更新日期:1997-03-01 00:00:00

  • Relationship of virulence factor expression to evolved virulence in mouse-passaged Cryptococcus neoformans lines.

    abstract::Serial passage of Cryptococcus neoformans in mice increases virulence relative to the nonpassaged line. Postpassaged lines showed no difference in the expression of most known virulence factors, with the exception that the more virulent lines had smaller capsules in vitro. These data imply that other mechanisms of vir...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.10.7047-7050.2005

    authors: McClelland EE,Perrine WT,Potts WK,Casadevall A

    更新日期:2005-10-01 00:00:00

  • Characterization of novel staphylococcal enterotoxin-like toxin type P.

    abstract::We investigated the biological properties of a novel staphylococcal enterotoxin (SE)-like toxin type P (SElP). SElP induced a substantial proliferative response and the production of cytokines interleukin-2, gamma interferon, tumor necrosis factor alpha, and interleukin-4 from human T cells when administered at a conc...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.9.5540-5546.2005

    authors: Omoe K,Imanishi K,Hu DL,Kato H,Fugane Y,Abe Y,Hamaoka S,Watanabe Y,Nakane A,Uchiyama T,Shinagawa K

    更新日期:2005-09-01 00:00:00

  • Levels of salivary lysozyme, lactoperoxidase, and lactoferrin in diabetic hamsters.

    abstract::In an attempt to clarify the mechanism(s) of increased susceptibility to oral infection in diabetics, we examined the levels of salivary antibacterial factors, including lysozyme, lactoperoxidase, and lactoferrin, in diabetic hamsters whose condition was induced with streptozotocin. Saliva was collected from these ham...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.48.2.389-394.1985

    authors: Muratsu K,Morioka T

    更新日期:1985-05-01 00:00:00