Ultrastructural study of adherence to and penetration of cultured cells by two invasive Escherichia coli strains isolated from infants with enteritis.

Abstract:

:The adherence of invasive Escherichia coli strains 444-3 and 469-3 to human erythrocytes and to cultured HeLa and HEp-2 cells has been examined by electron microscopy. Bacteria elaborating type 1 fimbriae, glycocalyces , and nonfimbrial mannose-resistant hemagglutinins specific for human erythrocytes were identified in cultures of both strains, and each of these different bacterial surface components appeared to be involved in attachment of 444-3 and 469-3 to cultured epithelial cells or human erythrocytes (or to both). Both strains, which were isolated from infants with dysentery-like illness, penetrated cultured epithelial cells and existed within membrane-bounded intracellular vesicles. Mutants of 444-3 and 469-3 selected for deficiency in mannose-resistant hemagglutination did not adhere to or penetrate cultured cells. These ultrastructural studies demonstrate the complexity of the bacterial surface and show that E. coli strains 444-3 and 469-3 can elaborate several different adhesions , each of which could function to promote attachment to host intestinal epithelial cells. Mucosal invasion may also be an important virulence property of these strains.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Knutton S,Williams PH,Lloyd DR,Candy DC,McNeish AS

doi

10.1128/IAI.44.3.599-608.1984

subject

Has Abstract

pub_date

1984-06-01 00:00:00

pages

599-608

issue

3

eissn

0019-9567

issn

1098-5522

journal_volume

44

pub_type

杂志文章
  • Protective properties of rifampin-resistant rough mutants of Brucella melitensis.

    abstract::Vaccination against Brucella infections in animals is usually performed by administration of live attenuated smooth B. abortus strain S19 and B. melitensis strain Rev1. They are proven effective vaccines against B. abortus in cattle and against B. melitensis and B. ovis in sheep and goats, respectively. However, both ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.7.4198-4204.2005

    authors: Adone R,Ciuchini F,Marianelli C,Tarantino M,Pistoia C,Marcon G,Petrucci P,Francia M,Riccardi G,Pasquali P

    更新日期:2005-07-01 00:00:00

  • Regulation of surface presentation of IcsA, a Shigella protein essential to intracellular movement and spread, is growth phase dependent.

    abstract::After lysing the phagocytic vacuole, Shigella spp. accumulate filaments of polymerized actin on their surface at one pole, leading to the formation of actin tails that enable them to move through the cytoplasm. We have recently demonstrated that the Shigella protein IcsA is located at the pole that is adjacent to the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.12.5664-5668.1994

    authors: Goldberg MB,Theriot JA,Sansonetti PJ

    更新日期:1994-12-01 00:00:00

  • Plasmid-dependent attachment of Agrobacterium tumefaciens to plant tissue culture cells.

    abstract::Kinetic, microscopic, and biochemical studies show that virulent Ti (tumor inducing)-plasmid-containing strains of Agrobacterium attach to normal tobacco and carrot tissue culture cells. Kinetic studies showed that virulent strains of A. tumefaciens attach to the plant tissue culture cells in increasing numbers during...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.22.2.516-522.1978

    authors: Matthysse AG,Wyman PM,Holmes KV

    更新日期:1978-11-01 00:00:00

  • Endotoxin-induced tumor necrosis factor alpha synthesis in murine embryo fibroblasts.

    abstract::Murine embryo fibroblasts (MEF) were found to secrete tumor necrosis factor (TNF) in response to stimulation with endotoxin. Endotoxin-induced TNF production by MEF was inhibited by cycloheximide. However, reversal of the effect of this inhibitor on protein synthesis results in TNF being secreted in amounts equivalent...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.5.1630-1635.1993

    authors: Havell EA,Rogerson BJ

    更新日期:1993-05-01 00:00:00

  • Immunological and molecular characterization of Helicobacter felis urease.

    abstract::Urease activity has recently been shown to be an important virulence determinant for Helicobacter pylori, allowing it to survive the low pH of the stomach during colonization. Experimental murine infection with Helicobacter felis is now being used as a model for H. pylori infection to study the effects of vaccines, an...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.3.793-798.1994

    authors: Gootz TD,Perez-Perez GI,Clancy J,Martin BA,Tait-Kamradt A,Blaser MJ

    更新日期:1994-03-01 00:00:00

  • Protection against lethal challenge of BALB/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2.

    abstract::Monoclonal antibodies secreted by six hybridomas and recognizing antigenic sites on glycoproteins gC, gAB, gD, gE, and gF of herpes simplex virus type 2 were examined for their ability to protect BALB/c mice from lethal infection by the virus. Administration of monoclonal antibodies to individual glycoproteins intrape...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.37.3.1132-1137.1982

    authors: Balachandran N,Bacchetti S,Rawls WE

    更新日期:1982-09-01 00:00:00

  • Pure botulinum neurotoxin is absorbed from the stomach and small intestine and produces peripheral neuromuscular blockade.

    abstract::Clostridium botulinum serotype A produces a neurotoxin composed of a 100-kDa heavy chain and a 50-kDa light chain linked by a disulfide bond. This neurotoxin is part of a ca. 900-kDa complex, formed by noncovalent association with a single nontoxin, nonhemagglutinin subunit and a family of hemagglutinating proteins. P...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.9.4708-4712.1999

    authors: Maksymowych AB,Reinhard M,Malizio CJ,Goodnough MC,Johnson EA,Simpson LL

    更新日期:1999-09-01 00:00:00

  • Biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm Ancylostoma caninum.

    abstract::We report the cloning and expression of Ac-GST-1, a novel glutathione S-transferase from the adult hookworm Ancylostoma caninum, and its possible role in parasite blood feeding and as a vaccine target. The predicted Ac-GST-1 open reading frame contains 207 amino acids (mass, 24 kDa) and exhibited up to 65% amino acid ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.10.6903-6911.2005

    authors: Zhan B,Liu S,Perally S,Xue J,Fujiwara R,Brophy P,Xiao S,Liu Y,Feng J,Williamson A,Wang Y,Bueno LL,Mendez S,Goud G,Bethony JM,Hawdon JM,Loukas A,Jones K,Hotez PJ

    更新日期:2005-10-01 00:00:00

  • Nonspecific resistance against infection with Salmonella typhi and Salmonella typhimurium induced in mice by cord factor (trehalose-6,6'-dimycolate) and its analogues.

    abstract::Mice pretreated intraperitoneally with trehalose-6,6'-dimycolate (cord factor) were protected against an intraperitoneal challenge with Salmonella typhi strain Ty2 or Salmonella typhimurium strain SR 11. The nonspecific resistance to S. typhi and S. typhimurium was still detectable 7 and 14 days, respectively, after a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.14.5.1125-1129.1976

    authors: Yarkoni E,Bekierkunst A

    更新日期:1976-11-01 00:00:00

  • Humanized mice, a new model to study the influence of drug treatment on neonatal sepsis.

    abstract::Bacterial infection with group B Streptococcus (GBS) represents a prominent threat to neonates and fetuses in the Western world, causing severe organ damage and even death. To improve current therapeutic strategies and to investigate new approaches, an appropriate in vivo model to study the immune response of a human ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01235-12

    authors: Ernst W,Zimara N,Hanses F,Männel DN,Seelbach-Göbel B,Wege AK

    更新日期:2013-05-01 00:00:00

  • Experimental cryptococcosis in normal and B-cell-deficient mice.

    abstract::B-cell-deficient mice were prepared by administration of rabbit anti-mouse-mu antiserum to newborn animals within 12 h of birth onwards. Such immunodeficient animals, along with the normal controls, were infected intravenously with Cryptococcus neoformans. There was no difference in the mortality pattern, viable count...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.26.1.1-3.1979

    authors: Monga DP,Kumar R,Mohapatra LN,Malaviya AN

    更新日期:1979-10-01 00:00:00

  • Purification and properties of a 75-kilodalton major protein, an immunodominant surface antigen, from the oral anaerobe Bacteroides gingivalis.

    abstract::A 75-kilodalton major protein (75K protein) was purified to homogeneity from the cell lysate fraction and the envelope of Bacteroides gingivalis 381. The 75K protein was originally present in the outer membrane or the outermost part of this organism as a large, stable complex with an apparent molecular weight of about...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.11.3646-3652.1989

    authors: Yoshimura F,Watanabe K,Takasawa T,Kawanami M,Kato H

    更新日期:1989-11-01 00:00:00

  • Characterization of lectinlike surface components on Capnocytophaga ochracea ATCC 33596 that mediate coaggregation with gram-positive oral bacteria.

    abstract::The interactions between Capnocytophaga ochracea ATCC 33596 and Streptococcus sanguis H1, Actinomyces naeslundii PK984, or Actinomyces israelii PK16 are dependent on specific recognitions between heat-sensitive adhesins on C. ochracea and heat-stable structures (probably carbohydrate-containing receptors) on the surfa...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.5.1198-1202.1987

    authors: Weiss EI,London J,Kolenbrander PE,Kagermeier AS,Andersen RN

    更新日期:1987-05-01 00:00:00

  • Evidence on possible Mycoplasma etiology of aster yellows disease I. Suppression of symptom development in plants by antibiotics.

    abstract::Antibiotics suppressed development of aster yellows (AY) disease symptoms in plants of china aster [Callistephus chinensis (L.) Nees.] and annual chrysanthemum (Chrysanthemum carinatum, Schousb.). When inoculated chrysanthemum plants were treated by any of several techniques with tetracycline antibiotics or chloramphe...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.2.2.201-208.1970

    authors: Davis RE,Whitcomb RF

    更新日期:1970-08-01 00:00:00

  • Identification of the gene encoding the FhbB protein of Treponema denticola, a highly unique factor H-like protein 1 binding protein.

    abstract::The gene encoding the Treponema denticola factor H-like protein 1 (FHL-1) binding protein, FhbB, was recovered and characterized. Sequence conservation, expression, and properties of FhbB were analyzed. The identification of FhbB represents an important step in understanding the contribution of FHL-1 binding in T. den...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01458-06

    authors: McDowell JV,Frederick J,Stamm L,Marconi RT

    更新日期:2007-02-01 00:00:00

  • A Legionella pneumophila gene that promotes hemin binding.

    abstract::The ability to bind and utilize hemin is a trait common to many human pathogens. Nevertheless, the relationship between Legionella pneumophila, the agent of Legionnaires' disease, and hemin has received little attention. Thus, we explored the capacity of a virulent, serogroup 1 strain of L. pneumophila to bind hemin a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.3.842-848.1996

    authors: O'Connell WA,Hickey EK,Cianciotto NP

    更新日期:1996-03-01 00:00:00

  • Down regulation of Entamoeba histolytica virulence by monoxenic cultivation with Escherichia coli O55 is related to a decrease in expression of the light (35-kilodalton) subunit of the Gal/GalNAc lectin.

    abstract::Entamoeba histolytica virulence is related to a number of amebic components (lectins, cysteine proteinases, and amebapore) and host factors, such as intestinal bacterial flora. Trophozoites are selective in their interactions with bacteria, and the parasite recognition of glycoconjugates plays an important role in ame...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.5.2096-2102.1999

    authors: Padilla-Vaca F,Ankri S,Bracha R,Koole LA,Mirelman D

    更新日期:1999-05-01 00:00:00

  • Cytoadherence of Plasmodium falciparum-infected erythrocytes to human melanoma cell lines correlates with surface OKM5 antigen.

    abstract::OKM5 antigen and thrombospondin are currently under investigation as potential receptors on the surface of human monocytes, endothelial cells, and melanomas responsible for the cytoadherence of Plasmodium falciparum-infected erythrocytes. We have studies the binding capacity of six human melanoma cell lines and relate...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.11.2754-2758.1987

    authors: Panton LJ,Leech JH,Miller LH,Howard RJ

    更新日期:1987-11-01 00:00:00

  • Localization of high-molecular-weight adhesion proteins of nontypeable Haemophilus influenzae by immunoelectron microscopy.

    abstract::A family of high-molecular-weight (HMW) surface-exposed proteins important in the attachment of nontypeable Haemophilus influenzae (NTHi) to human epithelial cells was previously identified (J. W. St. Geme III, S. Falkow, and S. J. Barenkamp, Proc. Natl. Acad. Sci. USA 90:2875-2879, 1993). In the present investigation...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.10.4460-4468.1994

    authors: Bakaletz LO,Barenkamp SJ

    更新日期:1994-10-01 00:00:00

  • Effect on intraerythrocytic Anaplasma marginale of soluble factors from infected calf blood mononuclear cells.

    abstract::Blood mononuclear cells (lymphocytes and monocytes) were isolated from infected calves during in vivo control of acute anaplasmosis and cultured with Anaplasma marginale organisms. Supernatants from the cultures reduced the proportion of erythrocytes containing viable A. marginale in vitro, indicating that an antibody...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.11.4846-4849.1996

    authors: Wyatt CR,Davis WC,Knowles DP,Goff WL,Palmer GH,McGuire TC

    更新日期:1996-11-01 00:00:00

  • Outer membrane proteins as a carrier for detoxified lipooligosaccharide conjugate vaccines for nontypeable Haemophilus influenzae.

    abstract::Nontypeable Haemophilus influenzae (NTHi) is a common cause of otitis media and respiratory tract infections. Outer membrane proteins (OMP) and lipooligosaccharide (LOS) are major surface antigens of NTHi and potential vaccine candidates. De-O-acylated LOS (dLOS) or oligosaccharide (OS) was coupled to total OMP to for...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.10.5508-5513.1999

    authors: Wu TH,Gu XX

    更新日期:1999-10-01 00:00:00

  • Direct activation of human monocyte-derived macrophages by a bacterial glycoprotein extract inhibits the intracellular multiplication of virulent Legionella pneumophila serogroup 1.

    abstract::Intracellular multiplication of virulent Legionella pneumophila serogroup 1 was inhibited by human monocyte-derived macrophages activated by a glycoprotein extract of Klebsiella pneumoniae, RU 41.740. Macrophage cultures were infected with L. pneumophila in the presence of immune antibodies on day 7 of culture. Extrac...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.9.2234-2239.1987

    authors: Rajagopalan P,Dournon E,Vildé JL,Pocidalo JJ

    更新日期:1987-09-01 00:00:00

  • Anti-Pneumococcal Capsular Polysaccharide Antibody Response and CD5 B Lymphocyte Subsets.

    abstract::The role of CD19(+) CD5(+) and CD19(+) CD5(-) B cell subpopulations in the antibody response to pneumococcal capsular polysaccharides (caps-PSs) is controversial. In the present study, we evaluated the role of human CD19(+) CD5(+) and CD19(+) CD5(-) cell populations in the serotype-specific antibody response to caps-P...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00068-15

    authors: Moens L,Verbinnen B,Covens K,Wuyts G,Johnson M,Roalfe L,Goldblatt D,Meyts I,Bossuyt X

    更新日期:2015-07-01 00:00:00

  • Comparison of haemophilus influenzae type b interaction with respiratory mucosa organ cultures maintained with an air interface or immersed in medium.

    abstract::Haemophilus influenzae type b infection of immersed and air interface organ cultures for 24 h caused significant epithelial damage. Bacterial association with mucus, damaged epithelium, and unciliated cells was significantly higher in air interface than immersed organ cultures, and total bacterial association was 55.8...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.6.2353-2355.1996

    authors: Jackson AD,Cole PJ,Wilson R

    更新日期:1996-06-01 00:00:00

  • Identification of lipoprotein homologues of pneumococcal PsaA in the equine pathogens Streptococcus equi and Streptococcus zooepidemicus.

    abstract::Streptococcus equi and Streptococcus zooepidemicus are major etiological agents of upper and lower airway disease in horses. Despite the considerable animal suffering and economic burden associated with these diseases, the factors that contribute to the virulence of these equine pathogens have not been extensively inv...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.10.6048-6051.2000

    authors: Harrington DJ,Greated JS,Chanter N,Sutcliffe IC

    更新日期:2000-10-01 00:00:00

  • Antibodies to porin antigens of Salmonella typhi induced during typhoid infection in humans.

    abstract::Immunoglobulin G (IgG)- and IgM-specific antibody titers against Salmonella typhi Ty2 porins have been measured in 30 paired typhoid sera by enzyme-linked immunosorbent assay. These studies have found that IgG serum titers of acute and convalescent sera were 625 and 5,000 times higher, respectively than the control se...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.52.1.209-212.1986

    authors: Calderón I,Lobos SR,Rojas HA,Palomino C,Rodríguez LH,Mora GC

    更新日期:1986-04-01 00:00:00

  • A novel component different from endotoxin extracted from Prevotella intermedia ATCC 25611 activates lymphoid cells from C3H/HeJ mice and gingival fibroblasts from humans.

    abstract::A novel immunobiologically active fraction was prepared from a phenol-water extract of Prevotella intermedia ATCC 25611 by Sephadex G-100 column chromatography. The fraction consisted mainly of carbohydrate and protein and was devoid of fatty acid. The fraction showed high-molecular-weight bands (10,000 to 12,000) on ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.11.4531-4538.1997

    authors: Iki K,Kawahara K,Sawamura S,Arakaki R,Sakuta T,Sugiyama A,Tamura H,Sueda T,Hamada S,Takada H

    更新日期:1997-11-01 00:00:00

  • Expression of a Clostridium perfringens type IV pilin by Neisseria gonorrhoeae mediates adherence to muscle cells.

    abstract::Clostridium perfringens is an anaerobic, Gram-positive bacterium that causes a range of diseases in humans, including lethal gas gangrene. We have recently shown that strains of C. perfringens move across the surface of agar plates by a unique type IV pilus (TFP)-mediated social motility that had not been previously d...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00909-10

    authors: Rodgers K,Arvidson CG,Melville S

    更新日期:2011-08-01 00:00:00

  • Involvement of cells of hematopoietic origin in genetically determined resistance of Syrian hamsters to vesicular stomatitis virus.

    abstract::Susceptibility of Syrian hamsters of the inbred LSH and MHA strains to injection of as few as 10 plaque-forming units of vesicular stomatitis virus (VSV) was shown to occur only after intraperitoneal and intrapleural injection and not after injection of VSV intravenously, intranasally, or in the footpads. Despite the ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.34.2.540-549.1981

    authors: Fultz PN,Shadduck JA,Kang CY,Streilein JW

    更新日期:1981-11-01 00:00:00

  • Comparative analyses of a cystic fibrosis isolate of Bordetella bronchiseptica reveal differences in important pathogenic phenotypes.

    abstract::Bordetella bronchiseptica is a Gram-negative bacterium that infects and causes disease in a wide variety of animals. B. bronchiseptica also infects humans, thereby demonstrating zoonotic transmission. An extensive characterization of human B. bronchiseptica isolates is needed to better understand the distinct genetic ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01453-13

    authors: Sukumar N,Nicholson TL,Conover MS,Ganguly T,Deora R

    更新日期:2014-04-01 00:00:00