Abstract：
:Vascular endothelial growth factor (VEGF) can overcome a potential anti-angiogenic effect of TNF-alpha by inhibiting endothelial apoptosis induced by this cytokine. Soluble TNF-alpha receptor I (sTNFRI) is an extracellular domain of TNFRI and antagonizes the activity of TNF-alpha. Here we report that sTNFRI is able to stimulate the growth of endothelial cells not by antagonizing TNF-alpha. Exogenously added recombinant human sTNFRI stimulated significantly more cell growth of human umbilical venous endothelial cells (HUVEC) with a low dose (50-200 pg/ml) compared with smooth muscle cells. In contrast, monoclonal antibody against TNF-alpha did not stimulate growth of human HUVEC. The sTNFRI expression plasmid (pcDNA3.1 plasmid) was introduced into the cell culture using OPTI-MEM, lipofectin and transferrin. Growth of HUVEC transfected with sTNFRI vector also increased significantly compared with those transfected with control vector. HUVEC transfected with sTNFRI vector increased the extracellular domain of TNFRI mRNA levels, but did not affect the intracellular domain of TNFRI mRNA levels. Accumulation of sTNFRI significantly increased in conditioned medium from HUVEC transfected with sTNFRI vector compared with those transfected with control vector. HUVEC transfected with sTNFRI vector not only increased sTNFRI but also prevented shedding of sTNFRI from TNFRI. The TNF-alpha -induced internucleosomic fragmentation was also significantly prevented in HUVEC transfected with sTNFRI vector compared with those transfected with control vector. These results suggest that instead of growth factors such as VEGF, local transfection of the sTNFRI gene may have potential therapeutic value in vascular diseases in which TNF-alpha is also usually highly expressed.
journal_name
Atherosclerosisjournal_title
Atherosclerosisauthors
Sugano M,Tsuchida K,Tomita H,Makino Ndoi
10.1016/s0021-9150(01)00684-0keywords:
subject
Has Abstractpub_date
2002-05-01 00:00:00pages
77-84issue
1eissn
0021-9150issn
1879-1484pii
S0021-9150(01)00684-0journal_volume
162pub_type
杂志文章相关文献
ATHEROSCLEROSIS文献大全abstract：:SR-12813 inhibits cholesterol biosynthesis in Hep G2 cells via an enhanced degradation of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase. Here we also show that SR-12813 inhibits cholesterol biosynthesis in vivo. A sterol balance study was performed in normolipemic beagle dogs. The dogs were given SR-12813 ...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/s0021-9150(97)00131-7
更新日期：1997-09-01 00:00:00
abstract：:Altered activity of matrix metalloproteinases (MMPs) is implicated in the vascular remodeling of hypertension. We examined whether increased MMP-2 expression/activity plays a role in the vascular remodeling and dysfunction found in the two-kidney, one-clip (2K-1C) hypertension. Sham operated or 2K-1C hypertension rats...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2007.10.011
更新日期：2008-06-01 00:00:00
abstract：OBJECTIVE:25-Hydroxycholesterol (25HC) and its sulfated metabolite, 25-hydroxycholesterol-3-sulfate (25HC3S), regulate certain aspects of lipid metabolism in opposite ways. Hence, the enzyme for the biosynthesis of 25HC3S, oxysterol sulfotransferase (SULT2B1b), may play a crucial role in regulating lipid metabolism. We...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2010.11.021
更新日期：2011-02-01 00:00:00
abstract：:Lysosomal acid lipase deficiency (LAL-D) is a rare autosomal recessive lysosomal storage disease caused by deleterious mutations in the LIPA gene. The age at onset and rate of progression vary greatly and this may relate to the nature of the underlying mutations. Patients presenting in infancy have the most rapidly pr...
journal_title：Atherosclerosis
pub_type： 杂志文章,评审
doi：10.1016/j.atherosclerosis.2014.04.003
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVE:We investigated whether early atherosclerosis before calcified plaque appeared as assessed by carotid intima-media thickness (IMT) was associated with levels of the major carotenoids, and assessed the associations among these serum carotenoids, carotid IMT, and arterial stiffness. METHODS:The case-control st...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2011.08.006
更新日期：2011-12-01 00:00:00
abstract：:Glycoprotein VI is a platelet collagen receptor binding to subendothelial collagen after a rupture of an atherosclerotic plaque. The GPVI gene is polymorphic with several SNPs and the T13254C polymorphism predicting amino acid substitution (serine to proline) has been associated with the risk of MI in a preliminary st...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2004.03.021
更新日期：2004-09-01 00:00:00
abstract：BACKGROUND:CD163 is a new potential scavenger receptor of Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) which elicits diverse biologic actions involved in atherosclerosis. We have analyzed the importance of TWEAK-CD163 interaction in atherosclerosis. METHODS:TWEAK and CD163 interaction was studied in cu...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2009.04.033
更新日期：2009-11-01 00:00:00
abstract：:The relationships among platelet membrane fluidity, arterial thrombosis tendency, and platelet aggregation and prostanoid formation were investigated after feeding rats diets enriched in (n-6) or (n-3) polyunsaturated fatty acids. For at least 8 weeks, rats were fed a control diet containing 5 energy % (en%) sunflower...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(86)90101-2
更新日期：1986-12-01 00:00:00
abstract：:The cell-adhesion molecule CD44 likely participates in atherosclerosis development. We have shown previously that pro-inflammatory cytokines affect CD44 expression. Therefore, this work examined the role of elevated CD44 levels in human macrophages. Macrophages from human atherosclerotic subjects (n=15) showed elevate...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2006.03.020
更新日期：2007-02-01 00:00:00
abstract：:Deficit sialylation of vascular endothelium, circulating cells and macromolecules has been associated with the development of atherosclerosis. On the other hand, an elevated serum level of sialic acid is a long-lasting marker of atherosclerosis and complications from atherosclerosis. One may speculate that the inverse...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2007.03.011
更新日期：2007-06-01 00:00:00
abstract：:No evidence of premature vascular disease is found in apolipoprotein A-I(Milano) (apoA-I(M)) human carriers, despite very low high density lipoprotein (HDL) cholesterol levels. Whether apoA-I(M) may impart a "gain of function" in atherosclerosis protection compared to wild-type apoA-I is hotly debated. To address this...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2005.03.008
更新日期：2005-12-01 00:00:00
abstract：:Proton NMR spectroscopy as a means to quantify lipoprotein subclasses has received wide clinical interest. The experimental part is a fast routine procedure that contrasts favourably to other lipoprotein measurement protocols. The difficulties in using (1)H NMR, however, are in uncovering the subclass specific informa...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2006.04.020
更新日期：2007-02-01 00:00:00
abstract：:Role of enterohepatic cholesterol metabolism in obesity-induced increase of cholesterol synthesis was studied in healthy lean (BMI <24) and overweight (BMI >31) subjects by measuring serum lipids (including plant sterols, cholestanol and cholesterol precursors), cholesterol absorption % (double-label method), sterol b...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/s0021-9150(00)00404-4
更新日期：2000-11-01 00:00:00
abstract：:The pathological remodeling of the arterial wall in atherosclerosis involves protease activities, which play a major role in complications, through plaque rupture. Here, we investigated the release of active proteases by human carotid plaques in relation to (1) the degree of lesion complexity and (2) their compartment...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2006.04.011
更新日期：2007-03-01 00:00:00
abstract：BACKGROUND AND AIMS:The exact mechanism of premature atherosclerosis in diabetes is still unclear. Inappropriate activation of the renin-aldosterone-angiotensin system may be an important risk factor for cardiovascular disease. We investigated whether renin and aldosterone are associated with vasoactive peptides midreg...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2016.07.905
更新日期：2016-09-01 00:00:00
abstract：:This is a study to define the profile of chemokine receptors expressed on isolated infiltrating lymphocytes in human abdominal aortic aneurysms (AAAs), and to examine their role in lymphoid recruitment. AAA T-lymphocytes were CXCR4-positive, CCR7-negative and partially CXCR3 and CCR5-positive. Functionally, AAA T-cell...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2007.12.043
更新日期：2008-10-01 00:00:00
abstract：:We studied the aortic histology of 28 Macaca nigra males and females, from 6 to more than 20 years old, normal and manifesting various degrees of spontaneous diabetes. Correlations of several metabolic and hormonal indicators of diabetes severity with gross and microscopic findings in the aortas demonstrated direct as...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(84)90158-8
更新日期：1984-07-01 00:00:00
abstract：OBJECTIVE:To summarize the evidence with respect to sugar sweetened beverages (SSBs) consumption and risk of coronary heart disease (CHD) and to recommend field standards for future analysis on this topic. METHODS:We searched for articles published up to February 2013 through PubMed, EMbase, and Cochrane Library Datab...
journal_title：Atherosclerosis
pub_type： 杂志文章,meta分析
doi：10.1016/j.atherosclerosis.2014.01.037
更新日期：2014-05-01 00:00:00
abstract：:Cholesterol lowering in both primary and secondary prevention has been clearly demonstrated to lower coronary morbidity and, in secondary prevention, to lower coronary mortality as well. Putative dangers of cholesterol lowering remain unproven. Population studies linking low cholesterol to noncoronary mortalities do n...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(94)90159-7
更新日期：1994-08-01 00:00:00
abstract：:We have analyzed the development of aortic and coronary atherosclerosis over 40 years in native and non-native males of Yakutsk by comparing the data obtained in 1963-1965, 1985-1987 and 2001-2003. The research was carried out in compliance with the WHO program for the atherosclerosis epidemiology studies using autops...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2006.04.002
更新日期：2007-02-01 00:00:00
abstract：:Vascular endothelial cell death contributes to the progression of atherosclerotic lesion, and several transcriptional regulators are involved in the process. Activating transcription factor 3/liver regenerating factor-1 (ATF3/LRF-1), a stress-inducible transcriptional repressor, was shown to be highly expressed in vas...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/s0021-9150(01)00639-6
更新日期：2002-04-01 00:00:00
abstract：:Polar coordinate mapping was used to determine the rate of growth of individual sudanophilic lesions on the aortic wall around several major branches of the aortae of cholesterol fed rabbits. Four groups, with 6 8-month old male albino white rabbits in each, were used in the study. One group served as a control and th...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(76)90043-5
更新日期：1976-10-01 00:00:00
abstract：:To investigate the association between time from diagnosis of type 2 diabetes to initiation of insulin therapy (pre-exogenous insulin period) and intima-media thickness (IMT) of the common carotid artery (CCA), a cross-sectional analysis was conducted among 98 males and 193 females (90% Hispanic) with type 2 diabetes,...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/s0021-9150(03)00291-0
更新日期：2003-10-01 00:00:00
abstract：:The effect of short-term dietary restriction on plasma cholesterol and cholesterol balance was examined in young While Carneau pigeons. Dietary restriction of 1 month increased plasma cholesterol significantly and subsequently decreased to initial levels at 3 months. The fecal excretion of neutral sterols was signific...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(76)90143-x
更新日期：1976-09-01 00:00:00
abstract：BACKGROUND:In coronary artery disease (CAD), concomitant peripheral arterial disease (PAD) entails more severe coronary atherosclerosis. We investigated whether the inflammatory status of affected limbs impairs coronary artery endothelial function (CAEF). METHODS:We measured the neutrophil myeloperoxidase content (NMP...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2008.01.014
更新日期：2008-12-01 00:00:00
abstract：:Animal models are used extensively in the ongoing investigation of a possible causal link between Chlamydia pneumoniae infection and conditions such as asthma and cardiovascular disease. Respiratory infections have been studied in monkeys, while mouse and rabbit models have been used to study both respiratory and card...
journal_title：Atherosclerosis
pub_type： 杂志文章,评审
doi：10.1016/s0021-9150(98)00115-4
更新日期：1998-10-01 00:00:00
abstract：:The aim of this work was to study how CETP expression affects whole body cholesterol homeostasis. Thus, tissue uptake and plasma removal rates of labeled HDL-cholesteryl ester (CE), VLDL secretion rates, and biliary lipid secretion and fecal bile acid content were compared between human CETP transgenic (Tg) and non-tr...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/j.atherosclerosis.2006.05.036
更新日期：2007-04-01 00:00:00
abstract：:Patients with homozygous familial hypercholesterolemia (FH), as a result of the increased levels and prolonged residence time of low density lipoprotein (LDL) in plasma, have a strong tendency toward accumulation of LDL-cholesterol in the arterial wall, causing premature atherosclerosis. This phenomenon may enhance pe...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(95)05612-2
更新日期：1995-12-01 00:00:00
abstract：:The effect of cage population density on plasma lipids and the development of atherosclerosis was examined in female C57BL/6 mice. Mice were housed at a density of one, two or five animals per cage and fed an atherogenic diet for 28 weeks. Subsequently, the animals were bled, sacrificed, the hearts removed and the ext...
journal_title：Atherosclerosis
pub_type： 杂志文章
doi：10.1016/0021-9150(94)05502-a
更新日期：1995-05-01 00:00:00
abstract：:We investigated the modulating effect of serum total triglycerides on the lipid composition of various lipoproteins, and on the response to gemfibrozil treatment. This placebo controlled study was conducted blind in 60 participants of the Helsinki Heart Study. An inverse relationship was observed between cholesterol c...
journal_title：Atherosclerosis
pub_type： 临床试验,杂志文章
doi：10.1016/0021-9150(90)90054-m
更新日期：1990-02-01 00:00:00
