Abstract：
:Antisuppressor mutations in the eEF1A gene of Podospora anserina were previously shown to impair ascospore formation, to drastically increase life span, and to permit the development of the Crippled Growth degenerative process. Here, we show that eEF1A controls ascospore formation through accuracy level maintenance. Examination of antisuppressor mutant perithecia reveals two main cytological defects, mislocalization of spindle and nuclei and nuclear death. Antisuppression levels are shown to be highly dependent upon both the mutation site and the suppressor used, precluding any correlation between antisuppression efficiency and severity of the sporulation impairment. Nevertheless, severity of ascospore differentiation defect is correlated with resistance to paromomycin. We also show that eEF1A controls fruiting body formation and longevity through a mechanism(s) different from accuracy control. In vivo, GFP tagging of the protein in a way that partly retains its function confirmed earlier cytological observation; i.e., this factor is mainly diffuse within the cytosol, but may transiently accumulate within nuclei or in defined regions of the cytoplasm. These data emphasize the fact that the translation apparatus exerts a global regulatory control over cell physiology and that eEF1A is one of the key factors involved in this monitoring.
journal_name
Geneticsjournal_title
Geneticsauthors
Silar P,Lalucque H,Haedens V,Zickler D,Picard Mkeywords:
subject
Has Abstractpub_date
2001-08-01 00:00:00pages
1477-89issue
4eissn
0016-6731issn
1943-2631journal_volume
158pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Mutations in genes of Saccharomyces cerevisiae that code for proteins that interact with beta-tubulin were sought by screening for unlinked mutations that fail to complement mutations in the single beta-tubulin-encoding gene (TUB2). Among the first three noncomplementing mutations examined, two are linked to TUB2 whil...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:Next generation sequencing (NGS) is about to revolutionize genetic analysis. Currently NGS techniques are mainly used to sequence individual genomes. Due to the high sequence coverage required, the costs for population-scale analyses are still too high to allow an extension to nonmodel organisms. Here, we show that NG...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.114397
更新日期：2010-09-01 00:00:00
abstract：:Mutagenic DNA repair (MDR) employs low-fidelity DNA polymerases capable of replicating past DNA lesions resulting from exposure to high-energy ultraviolet radiation (UVR). MDR confers UVR tolerance and activation initiates a transient mutator phenotype that may provide opportunities for adaptation. To investigate the ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.096131
更新日期：2009-01-01 00:00:00
abstract：:There have been several attempts to estimate the average dominance (ratio of heterozygous to homozygous effects) of spontaneous deleterious mutations in Drosophila melanogaster, but these have given inconsistent results. We investigated whether transposable element (TE) insertions have higher average dominance for egg...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-04-01 00:00:00
abstract：:Knowledge of the rate and fitness effects of mutations is essential for understanding the process of evolution. Mutations are inherently difficult to study because they are rare and are frequently eliminated by natural selection. In the ciliate Tetrahymena thermophila, mutations can accumulate in the germline genome w...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.153536
更新日期：2013-10-01 00:00:00
abstract：:Mutants resistant to selenomethionine were isolated, and their properties studied. Mapping studies indicate that the mutation sites are located near the eth-1(r) locus in linkage group I, about ten map units away from the mating type locus. The sites of new mutation are either allelic to or very close to eth-1(r). The...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-08-01 00:00:00
abstract：:Over 100 regulatory mutations linked to the histidine (his) operon of S. typhimurium have been isolated. They all map in a region estimated to be several hundred base pairs in length located at one end of the his operon ("the hisO region"). The mutations are located at sixteen recombinationally separable sites or are ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-10-01 00:00:00
abstract：:We have investigated meiotic changes in CAG repeat tracts embedded in a yeast chromosome. Repeat tracts undergo either conversion events between homologs or expansion and contraction events that appear to be confined to a single chromatid. We did not find evidence for conversion of tract interruptions or excess exchan...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:Studies in the fly, Drosophila melanogaster, have revealed that several signaling pathways are important for the regulation of growth. Among these, the insulin receptor/phosphoinositide 3-kinase (PI3K) pathway is remarkable in that it affects growth and final size without disturbing pattern formation. We have used a s...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.045021
更新日期：2005-10-01 00:00:00
abstract：:Mutations in ilvO, the operator site for operon A of the ilv region of E. coli K-12, are reported to cause a cis dominant derepression of the operon A gene products and to map between ilvC and ilvA. Studies reported here demonstrate that the F25 episome which does not carry the region between ilvC and ilvA but only il...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：:Mobile P elements in Drosophila melanogaster cause hybrid dysgenesis if their mobility is not repressed. One type of repression, termed P cytotype, is a complex interaction between chromosomes carrying P elements and cytoplasm and is transmitted through the cytoplasm only of females. Another type of repression is foun...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:Renal agenesis (RA) is one of the more extreme examples of congenital anomalies of the kidney and urinary tract (CAKUT). Bilateral renal agenesis is almost invariably fatal at birth, and unilateral renal agenesis can lead to future health issues including end-stage renal disease. Genetic investigations have identified...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.1125
更新日期：2017-09-01 00:00:00
abstract：:Condensins are evolutionarily conserved protein complexes that are required for chromosome segregation during cell division and genome organization during interphase. In Caenorhabditis elegans, a specialized condensin, which forms the core of the dosage compensation complex (DCC), binds to and represses X chromosome t...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302254
更新日期：2019-07-01 00:00:00
abstract：:Two independent isolates of Saccharomyces cerevisiae lacking hexokinase activity (EC 2.7.1.1) are described. Both mutant strains grow on glucose but are unable to grow on fructose, and contain two mutant genes hxk1 and hxk2 each. The mutations are recessive and noncomplementing. Genetic analysis suggests that these tw...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-08-01 00:00:00
abstract：:Cyclin E together with its kinase partner Cdk2 is a critical regulator of entry into S phase. To identify novel genes that regulate the G1- to S-phase transition within a whole animal we made use of a hypomorphic cyclin E mutation, DmcycEJP, which results in a rough eye phenotype. We screened the X and third chromosom...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.026617
更新日期：2004-09-01 00:00:00
abstract：:The specification of floral organ identity in the higher dicots depends on the function of a limited set of homeotic genes, many of them members of the MADS-box gene family. Two such genes, APETALA3 (AP3) and PISTILLATA (PI), are required for petal and stamen identity in Arabidopsis; their orthologs in Antirrhinum exh...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:Kinesin-1 is a motor protein that moves stepwise along microtubules by employing dimerized kinesin heavy chain (Khc) subunits that alternate cycles of microtubule binding, conformational change, and ATP hydrolysis. Mutations in the Drosophila Khc gene are known to cause distal paralysis and lethality preceded by the o...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.140798
更新日期：2012-09-01 00:00:00
abstract：:Transcription of the Saccharomyces MAL structural genes is induced 40-fold by maltose and requires the MAL-activator and maltose permease. To identify additional players involved in regulating MAL gene expression, we carried out a genetic selection for MAL constitutive mutants. Strain CMY4000 containing MAL1 and integ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.029611
更新日期：2004-10-01 00:00:00
abstract：:The availability of genomewide dense markers brings opportunities and challenges to breeding programs. An important question concerns the ways in which dense markers and pedigrees, together with phenotypic records, should be used to arrive at predictions of genetic values for complex traits. If a large number of marke...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.101501
更新日期：2009-05-01 00:00:00
abstract：:The transmission of transposed Ac elements in progeny derived by self-pollination of ten transformed tomato plants has been examined by Southern hybridization analysis. We show that six of these primary transformants have transmitted a transposed Ac to at least one progeny. One of the families was segregating for at l...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:The change in the frequency of a rare mutant allele under constant sex-differentiated viability selection in an infinite, partial full-sib mating population is studied. The diplo-diploid and haplo-diploid polygynous models are considered with a Poisson distribution for the number of offspring produced by every mated f...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.039024
更新日期：2005-09-01 00:00:00
abstract：:TE146 is a giant transposon of Drosophila melanogaster. It carries two copies of the white and roughest genes, normally found on the X chromosome. The structure of this transposon has been studied at the molecular level. TE146 may transpose to new chromosome positions, excise and be lost from the genome or undergo int...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:The hitchhiking model of population genetics predicts that an allele favored by Darwinian selection can replace haplotypes from the same locus previously established at a neutral mutation-drift equilibrium. This process, known as "selective sweep," was studied by comparing molecular variation between the polymorphic I...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:The third hypervariable (V3) region of the HIV-1 gp120 protein is responsible for many aspects of viral infectivity. The tertiary structure of the V3 loop seems to influence the coreceptor usage of the virus, which is an important determinant of HIV pathogenesis. Hence, the information about preferred conformations of...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.051508
更新日期：2006-03-01 00:00:00
abstract：:The protist Physarum polycephalum is useful for analysis of several aspects of cellular and developmental biology. To expand the opportunities for experimental analysis of this organism, we have developed a method for gene replacement. We transformed Physarum amoebae with plasmid DNA carrying a mutant allele, ardD del...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Preliminary studies with restriction fragment length polymorphisms of mitochondrial DNA (mtDNA) in natural populations of Drosophila melanogaster revealed considerable variation in terms of nucleotide sequence and overall size. In this report we present data from more isofemale lines and more restriction enzymes, and ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
abstract：:We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verifi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.076216
更新日期：2007-09-01 00:00:00
abstract：:We have employed a system that utilizes homologous pairs of human DNA-derived yeast artificial chromosomes (YACs) as marker chromosomes to assess the specific role(s) of conserved centromere DNA elements (CDEI, CDEII and CDEIII) in meiotic chromosome disjunction fidelity. Thirteen different centromere (CEN) mutations ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：:DNA sequence variation in a 1410-bp region including the Cu,Zn Sod locus was examined in 41 homozygous lines of Drosophila melanogaster. Fourteen lines were from Barcelona, Spain, 25 were from California populations and the other two were from laboratory stocks. Two common electromorphs, SODS and SODF, are segregating...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Lineage specification in early development is the basis for the exquisitely precise body plan of multicellular organisms. It is therefore critical to understand cell fate decisions in early development. Moreover, for regenerative medicine, the accurate specification of cell types to replace damaged/diseased tissue is ...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.119.302661
更新日期：2019-12-01 00:00:00
