Abstract：
:The transmission of transposed Ac elements in progeny derived by self-pollination of ten transformed tomato plants has been examined by Southern hybridization analysis. We show that six of these primary transformants have transmitted a transposed Ac to at least one progeny. One of the families was segregating for at least two different insertion events. In five of ten families, progeny were detected that contained a transposed Ac but no donor T-DNA sequences, indicating that a recombination event occurred between the original and new Ac insertion site. Somatic transposition of Ac as late as the R2 generation is evidenced. One family contained an empty donor site fragment but Ac was not detected in either the parent or progeny, indicating Ac was lost in this population early in regeneration. While four of ten families were segregating for aberrant phenotypes, there was no evidence that the mutated gene was linked to a transposed Ac.
journal_name
Geneticsjournal_title
Geneticsauthors
Belzile F,Lassner MW,Tong Y,Khush R,Yoder JIsubject
Has Abstractpub_date
1989-09-01 00:00:00pages
181-9issue
1eissn
0016-6731issn
1943-2631journal_volume
123pub_type
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