Abstract:
:We have employed a system that utilizes homologous pairs of human DNA-derived yeast artificial chromosomes (YACs) as marker chromosomes to assess the specific role(s) of conserved centromere DNA elements (CDEI, CDEII and CDEIII) in meiotic chromosome disjunction fidelity. Thirteen different centromere (CEN) mutations were tested for their effects on meiotic centromere function. YACs containing a wild-type CEN DNA sequence segregate with high fidelity in meiosis I (99% normal segregation) and in meiosis II (96% normal segregation). YACs containing a 31-bp deletion mutation in centromere DNA element II (CDEII delta 31) in either a heterocentric (mutant/wild type), homocentric (mutant/mutant) or monosomic (mutant/--) YAC pair configuration exhibited high levels (16-28%) of precocious sister-chromatid segregation (PSS) and increased levels (1-6%) of nondisjunction meiosis I (NDI). YACs containing this mutation also exhibit high levels (21%) of meiosis II nondisjunction. Interestingly, significant alterations in homolog recombination frequency were observed in the exceptional PSS class of tetrads, suggesting unusual interactions between prematurely separated sister chromatids and their homologous nonsister chromatids. We also have assessed the meiotic segregation effects of rare gene conversion events occurring at sites located immediately adjacent to or distantly from the centromere region. Proximal gene conversion events were associated with extremely high levels (60%) of meiosis I segregation errors (including both PSS and NDI), whereas distal events had no apparent effect. Taken together, our results indicate a critical role for CDEII in meiosis and underscore the importance of maintaining sister-chromatid cohesion for proper recombination in meiotic prophase and for proper disjunction in meiosis I.
journal_name
Geneticsjournal_title
Geneticsauthors
Sears DD,Hegemann JH,Shero JH,Hieter Psubject
Has Abstractpub_date
1995-03-01 00:00:00pages
1159-73issue
3eissn
0016-6731issn
1943-2631journal_volume
139pub_type
杂志文章相关文献
GENETICS文献大全abstract::During selection for protein content in mice at the Technical University of Berlin, individuals showing high protein content and a compact exterior were noted. Animals showing this "Compact" phenotype were separated to form a new line. The present investigations were carried out on a Hungarian subpopulation of this li...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Crossovers (COs) between homologous chromosomes are critical for meiotic chromosome segregation and form in the context of the synaptonemal complex (SC), a meiosis-specific structure that assembles between aligned homologs. During Caenorhabditis elegans meiosis, central region components of the SC (SYP proteins) are e...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302625
更新日期:2019-10-01 00:00:00
abstract::As millions of single-nucleotide polymorphisms (SNPs) have been identified and high-throughput genotyping technologies have been rapidly developed, large-scale genomewide association studies are soon within reach. However, since a genomewide association study involves a large number of SNPs it is therefore nearly impo...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.060491
更新日期:2006-11-01 00:00:00
abstract::Dauer formation in Caenorhabditis elegans is induced by chemosensation of high levels of a constitutively secreted pheromone. Seven genes defined by mutations that confer a dauer-formation constitutive phenotype (Daf-c) can be congruently divided into two groups by any of three criteria. Group 1 genes (daf-11 and daf-...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::Heterochromatin protein 1a (HP1a) is a highly conserved and versatile epigenetic factor that can both silence and activate transcription. However, the function of HP1a in development has been underinvestigated. Here, we report the role of maternal HP1a in producing maternal transcripts that drive early Drosophila embr...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301704
更新日期:2019-01-01 00:00:00
abstract::Two composite multiple regression-interval mapping analyses were performed to identify candidate quantitative trait loci (QTL) affecting components of wing shape in Drosophila melanogaster defined by eight relative warp-based measures. A recombinant inbred line design was used to map QTL for the shape of two intervein...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::Due to issues of practicality and confidentiality of genomic data sharing on a large scale, typically only meta- or mega-analyzed genome-wide association study (GWAS) summary data, not individual-level data, are publicly available. Reanalyses of such GWAS summary data for a wide range of applications have become more ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300813
更新日期:2018-06-01 00:00:00
abstract::Various measures have been proposed for characterizing the statistical association that arises between alleles at different loci. Hedrick has compared these measures with the standardized measure D' proposed by Lewontin on the grounds that this latter measure is independent of allele frequency. Although D' has the sam...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::Correlation and regression analyses indicate that isofemale strains extracted from a population of the parasitic wasp, Nasonia vitripennis, differ in the fit of their second sex ratios (those produced in previously parasitized hosts) to the predictions of the theory of optimal facultative sex ratio adjustment. Under t...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract::The vacuolar hydrolase protease B in Saccharomyces cerevisiae is synthesized as an inactive precursor (Prb1p). The precursor undergoes post-translational modifications while transiting the secretory pathway. In addition to N- and O-linked glycosylations, four proteolytic cleavages occur during the maturation of Prb1p....
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract::Hybrid incompatibility can result from gene misregulation produced by divergence in trans-acting regulatory factors and their cis-regulatory targets. However, change in trans-acting factors may be constrained by pleiotropy, which would in turn limit the evolution of incompatibility. We employed a mechanistically expli...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.171397
更新日期:2014-12-01 00:00:00
abstract::The first five steps in de novo purine biosynthesis are involved in the formation of the 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) moiety of thiamine. We show here that the first enzyme in de novo purine biosynthesis, PurF, is required for thiamine synthesis during aerobic growth on some but not other carbon s...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::The LmR1 locus, which controls seedling resistance to the blackleg fungus Leptosphaeria maculans in the Brassica napus cultivar Shiralee, was positioned on linkage group N7. Fine genetic mapping in a population of 2500 backcross lines identified three molecular markers that cosegregated with LmR1. Additional linkage m...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.049098
更新日期:2005-12-01 00:00:00
abstract::The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chrom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.034801
更新日期:2004-10-01 00:00:00
abstract::Meiotically driven sex chromosomes can quickly spread to fixation and cause population extinction unless balanced by selection or suppressed by genetic modifiers. We report results of genetic analyses that demonstrate that extreme female-biased sex ratios in two sister species of stalk-eyed flies, Cyrtodiopsis dalmann...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::Tetrazolium oxidase from the blood and liver of rainbow trout was found to be genetically polymorphic. The inheritance pattern of the liver enzyme was compatible only with a one locus-two allele hypothesis. The enzymes in the blood while having an electrophoretically identical polymorphism could differ genotypically f...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-10-01 00:00:00
abstract::Chromosomal double-strand breaks (DSBs) that have only one end with homology to a donor duplex undergo repair by strand invasion followed by replication to the chromosome terminus (break-induced replication, BIR). Using a transformation-based assay system, it was previously shown that BIR could occur by several rounds...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.162297
更新日期:2014-04-01 00:00:00
abstract::To further understand how the nematode Caenorhabditis elegans defends itself against pathogen attack, we analyzed enhanced pathogen resistance (epr) mutants obtained from a forward genetic screen. We also examined several well-characterized sterile mutants that exhibit an Epr phenotype. We found that sterility and pat...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.083923
更新日期:2008-02-01 00:00:00
abstract::A model of the evolution of a transposable element family in a Mendelian host population is proposed that incorporates heritable phenotypic mutations in the elements. The temporal behavior of the numbers of mutant and wild-type elements is studied, and the expected extinction time of the transposable element family is...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::This FlyBook chapter summarizes the history and the current state of our understanding of the Wingless signaling pathway. Wingless, the fly homolog of the mammalian Wnt oncoproteins, plays a central role in pattern generation during development. Much of what we know about the pathway was learned from genetic and molec...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.117.300157
更新日期:2018-04-01 00:00:00
abstract::This study addresses three questions about the properties of recombination hotspots in Saccharomyces cerevisiae: How much DNA is required for double-strand-break (DSB) site recognition? Do naturally occurring DSB sites compete with each other in meiotic recombination? What role does the sequence located at the sites o...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::The Saccharomyces cerevisiae mitochondrial endonuclease I-SceI creates a double-strand break as the initiating step in the gene conversional transfer of the omega+ intron to omega- DNA. We have expressed a galactose-inducible synthetic I-SceI gene in the nucleus of yeast that also carries the I-SceI recognition site o...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-03-01 00:00:00
abstract::How many genes contribute to reproductive isolation between closely related species? We determined the number of genes located in the 9D-12B region of the Drosophila mauritiana X chromosome that cause hybrid male sterility in a D. simulans background. Previous low resolution studies suggested that a single hybrid ster...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::Self-incompatibility (SI) is a genetic system found in some hermaphrodite plants. Recognition of pollen by pistils expressing cognate specificities at two linked genes leads to rejection of self pollen and pollen from close relatives, i.e., to avoidance of self-fertilization and inbred matings, and thus increased outc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.127399
更新日期:2011-07-01 00:00:00
abstract::The Drosophila melanogaster Chd3 gene encodes a member of the CHD group of SNF2/RAD54 ATPases. CHD proteins are conserved from yeast to man and many are subunits of chromatin-remodeling complexes that facilitate transcription. Drosophila CHD3 proteins are not found in protein complexes, but as monomers that remodel ch...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.115121
更新日期:2010-07-01 00:00:00
abstract::Maize (Zea mays L.) serves as model plant for heterosis research and is the crop where hybrid breeding was pioneered. We analyzed genomic and phenotypic data of 1254 hybrids of a typical maize hybrid breeding program based on the important Dent × Flint heterotic pattern. Our main objectives were to investigate genome ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.165860
更新日期:2014-08-01 00:00:00
abstract::Isogenic lines, in which chromosomes sampled from natural populations of C. melanogaster are substituted into a common genetic background, were used to detect and partially characterize autosomal factors that affect the activities of the two pentose phosphate pathway enzymes, glucose 6-phosphate dehydrogenase (G6PD) a...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract::The TATA-binding protein (TBP) is composed of a highly conserved core domain sufficient for TATA-element binding and preinitiation complex formation as well as a highly divergent N-terminal region that is dispensable for yeast cell viability. In vitro, removal of the N-terminal region domain enhances TBP-TATA associat...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::We present theoretical explanations and show through simulation that the individual admixture proportion estimates obtained by using ancestry informative markers should be seen as an error-contaminated measurement of the underlying individual ancestry proportion. These estimates can be used in structured association t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.075408
更新日期:2007-07-01 00:00:00
abstract::Brassica carinata, an allotetraploid with B and C genomes, has a number of traits that would be valuable to introgress into B. napus. Interspecific hybrids were created between B. carinata (BBCC) and B. napus (AACC), using an advanced backcross approach to identify and introgress traits of agronomic interest from the ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.124925
更新日期:2011-03-01 00:00:00