Vasoactive intestinal peptide and related molecules induce nitrite accumulation in the extracellular milieu of rat cerebral cortical cultures.

Abstract:

:Nanomolar concentrations of vasoactive intestinal peptide (VIP), picomolar concentrations of stearyl-norleucine17-VIP (SNV) and femtomolar concentrations of NAPVSIPQ (NAP), an 8-amino-acid peptide derived from the VIP-responsive activity-dependent neuroprotective protein, provide broad neuroprotection. In rat cerebral cortical cultures, 10(-16)-10(-7) M NAP increased intracellular cyclic guanosine monophosphate (cGMP) (2.5-4-fold) and 10(-10) M NAP increased extracellular nitric oxide (NO) by 60%. In the same culture system, VIP and SNV (at micromolar concentrations) increased extracellular NO by 45-55%. The NAP dose required for cGMP increases correlated with the dose providing neuroprotection. However, the concentrations of NAP, SNV and VIP affecting NO production did not match the neuro-protective doses. Thus, NO may mediate part of the cell-cell interaction and natural maintenance activity of VIP/SNV/NAP, while cGMP may mediate neuroprotection.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Ashur-Fabian O,Giladi E,Furman S,Steingart RA,Wollman Y,Fridkin M,Brenneman DE,Gozes I

doi

10.1016/s0304-3940(01)01954-1

keywords:

subject

Has Abstract

pub_date

2001-07-20 00:00:00

pages

167-70

issue

3

eissn

0304-3940

issn

1872-7972

pii

S0304394001019541

journal_volume

307

pub_type

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