Abstract:
:Using a transgenic approach, we examined distal regulatory elements located in the IL-4 locus and the role of GATA-3 at these elements. The intergenic DNase I hypersensitive sites (HSS) showed strong enhancement, and the intronic enhancer (IE) and HS5/HS5a sites showed weaker enhancement of the IL-4 promoter. Elements in the 3' region of the IL-4 gene contributed to Th2 specificity. All individual enhancers were T cell activation dependent but not Th2 specific, with the exception of IE. However, when these distal elements were combined into a "minilocus," expression was strongly enhanced and Th2 specific. GATA-3 mediated strong enhancement of IL-4 promoter activity in Th1 cells when the promoter was embedded in the minilocus or linked to HSS and IE, demonstrating that GATA-3 acts through these elements to regulate IL-4 gene expression.
journal_name
Immunityjournal_title
Immunityauthors
Lee GR,Fields PE,Flavell RAdoi
10.1016/s1074-7613(01)00125-xkeywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
447-59issue
4eissn
1074-7613issn
1097-4180pii
S1074-7613(01)00125-Xjournal_volume
14pub_type
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