Abstract：
:The spindle assembly checkpoint-mediated mitotic arrest depends on proteins that signal the presence of one or more unattached kinetochores and prevents the onset of anaphase in the presence of kinetochore or spindle damage. In the presence of either damage, bub2 cells initiate a preanaphase delay but do not maintain it. Inappropriate sister chromatid separation in nocodazole-treated bub2 cells is prevented when mitotic exit is blocked using a conditional tem1(c) mutant, indicating that the preanaphase failure in bub2 cells is a consequence of events downstream of TEM1 in the mitotic exit pathway. Using a conditional bub2(tsd) mutant, we demonstrate that the continuous presence of Bub2 protein is required for maintaining spindle damage-induced arrest. BUB2 is not required to maintain a DNA damage checkpoint arrest, revealing a specificity for spindle assembly checkpoint function. In a yeast two-hybrid assay and in vitro, Bub2 protein interacts with the septin protein Cdc3, which is essential for cytokinesis. These data support the view that the spindle assembly checkpoint encompasses regulation of distinct mitotic steps, including a MAD2-directed block to anaphase initiation and a BUB2-directed block to TEM1-dependent exit.
journal_name
Geneticsjournal_title
Geneticsauthors
Krishnan R,Pangilinan F,Lee C,Spencer Fkeywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
489-500issue
2eissn
0016-6731issn
1943-2631journal_volume
156pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Natural selection favors alleles that increase the number of offspring produced by their carriers. But in a world that is inherently uncertain within generations, selection also favors alleles that reduce the variance in the number of offspring produced. If previous studies have established this principle, they have l...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.156067
更新日期：2014-01-01 00:00:00
abstract：:Genetic divergence in the form of the mandible is examined in ten inbred strains of mice. Several univariate and multivariate genetic distance estimates are given for the morphological data and these estimates are compared to measures of genealogical and molecular divergence. Highly significant divergence occurs among...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:Borde and colleagues reported that a reporter plasmid inserted at different genomic locations in Saccharomyces cerevisiae had different levels of meiotic recombination activity. We show that the level of recombination activity is very significantly correlated with the GC content of DNA sequences flanking the insertion...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：:The vertebrate limb serves as an experimental paradigm to study mechanisms that regulate development of the stereotypical skeletal elements. In this study, we simultaneously inactivated Sall4 using Hoxb6Cre and Plzf in mouse embryos, and found that their combined function regulates development of the proximal-anterior...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303069
更新日期：2020-05-01 00:00:00
abstract：:For multiallelic loci, standard measures of linkage disequilibrium provide an incomplete description of the correlation of variation at two loci, especially when there are different numbers of alleles at the two loci. We have developed a complementary pair of conditional asymmetric linkage disequilibrium (ALD) measure...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.165266
更新日期：2014-09-01 00:00:00
abstract：:Availability of animal protein for human consumption is very low in the developing countries mainly because of low productivity of existing livestock; ways and means to improve productivity through breeding are discussed and some basic issues requiring further research pointed out. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-09-01 00:00:00
abstract：:Comparison of population differentiation in neutral marker genes and in genes coding quantitative traits by means of F(ST) and Q(ST) indexes has become commonplace practice. While the properties and estimation of F(ST) have been the subject of much interest, little is known about the precision and possible bias in Q(S...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.044545
更新日期：2005-11-01 00:00:00
abstract：:We examined mutations that disrupt muscle activation in Caenorhabditis elegans. Fifteen of 17 of these genes were identified previously and we describe new mutations in three of them. We also describe mutations in two new genes, exp-3 and exp-4. We assessed the degree of defect in pharyngeal, body-wall, egg-laying, an...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chrom...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.034801
更新日期：2004-10-01 00:00:00
abstract：:We examined the effect of a single variant repeat on the stability of a 51-base pair (bp) microsatellite (poly GT). We found that the insertion stabilizes the microsatellite about fivefold in wild-type strains. The stabilizing effect of the variant base was also observed in strains with mutations in the DNA mismatch r...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:Recombination of phage lambda attachment sites occurs by sequential exchange of the DNA strands at two specific locations. The first exchange produces a Holliday structure, and the second resolves it to recombinant products. Heterology for base substitution mutations in the region between the two strand exchange point...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:Functional analyses of the Arabidopsis genome require analysis of the gametophytic generation, since approximately 10% of the genes are expressed in the male gametophyte and approximately 9% in the female gametophyte. Here we describe the genetic and molecular characterization of 67 Ds insertion lines that show reduce...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.090852
更新日期：2009-04-01 00:00:00
abstract：:We have genetically analyzed, cloned and physically mapped the modified cytosine-specific restriction determinants mcrA (rglA) and mcrB (rglB) of Escherichia coli K-12. The independently discovered Rgl and Mcr restriction systems are shown to be identical by three criteria: 1) mutants with the RglA- or RglB- phenotype...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-06-01 00:00:00
abstract：:Cyclic guanosine monophosphate (cGMP) is a key secondary messenger used in signal transduction in various types of sensory neurons. The importance of cGMP in the ASE gustatory receptor neurons of the nematode Caenorhabditis elegans was deduced by the observation that multiple receptor-type guanylyl cyclases (rGCs), en...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.152660
更新日期：2013-08-01 00:00:00
abstract：:We demonstrate that the previously described gene Androgen binding protein (Abp; Dlouhy and Karn, 1984) codes for the Alpha subunit of ABP and rename the locus Androgen binding protein alpha (Abpa). A study of recombinant inbred strains demonstrates that Abpa is located on chromosome 7 near Glucose phosphate isomerase...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:A model emphasizing the possible genetic role of tandem duplications of reverse repeats has been developed as an extension of Crick's (1971) general model for the chromosomes of higher organisms. Although developed initially (1) to explain why random differences in the control regions of individual gene loci might con...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-07-01 00:00:00
abstract：:The DNMT1 cytosine methyltransferase enzyme contains a large ∼300-aa intrinsically disordered domain (IDD) that we previously showed regulated DNA methylation patterns in mouse ES cells. Here we generated seven mouse lines with different mutations in the IDD. Homozygous mutant mice of five lines developed normally, wi...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.173609
更新日期：2015-02-01 00:00:00
abstract：:To identify novel genomic regions that regulate sex determination, we utilized the powerful C57BL/6J-Y(POS) (B6-Y(POS)) model of XY sex reversal where mice with autosomes from the B6 strain and a Y chromosome from a wild-derived strain, Mus domesticus poschiavinus (Y(POS)), show complete sex reversal. In B6-Y(POS), th...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.113.160259
更新日期：2014-07-01 00:00:00
abstract：:Competition among domesticated plants or animals can have a dramatic negative impact on yield of a stand or farm. The usual quantitative genetic model ignores these competitive interactions and could result in seriously incorrect breeding decisions and acerbate animal well-being. A general solution to this problem is ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.035956
更新日期：2005-07-01 00:00:00
abstract：:Dis3 encodes a conserved RNase that degrades or processes all RNA species via an N-terminal PilT N terminus (PIN) domain and C-terminal RNB domain that harbor, respectively, endonuclease activity and 3'-5' exonuclease activity. In Schizosaccharomyces pombe, dis3 mutations cause chromosome missegregation and failure in...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.187930
更新日期：2016-06-01 00:00:00
abstract：:A model of the evolution of a transposable element family in a Mendelian host population is proposed that incorporates heritable phenotypic mutations in the elements. The temporal behavior of the numbers of mutant and wild-type elements is studied, and the expected extinction time of the transposable element family is...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-02-01 00:00:00
abstract：:Drosophila dishevelled (dsh) functions in two pathways: it is necessary to transduce Wingless (Wg) signaling and it is required in planar cell polarity. To learn more about how Dsh can discriminate between these functions, we performed genetic screens to isolate additional dsh alleles and we examined the potential rol...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：:To understand the role of Los1p in pre-tRNA splicing, we sought los1 multicopy suppressors. We found SOL1 that suppresses both point and null LOS1 mutations. Since, when fused to the Ga14p DNA-binding domain, Los1p activates transcription, we tested whether Los1p regulates SOL1. We found that las1 mutants have deplete...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:Tetrahymena is a useful eukaryotic model for biochemistry and molecular cell biology studies. We previously demonstrated that targeted ectopic DNA elimination, also called co-Deletion (coDel), can be induced by the introduction of an internal eliminated sequence (IES)-target DNA chimeric construct. In this study, we d...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.178525
更新日期：2015-09-01 00:00:00
abstract：:The detection of adaptive evolution at the molecular level is of interest not only as an insight into the process of evolution but also because of its functional implications for genes of interest. Here, we present the first genomics approach to detecting positive selection operating on the Bos taurus lineage, an impo...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.039040
更新日期：2005-07-01 00:00:00
abstract：:Lethal and sterility mutations were accumulated in a cage population which was initiated with lethal- and sterility-free second chromosomes of D. melanogaster. It took about 2,000 days for the frequencies of these genes to reach equilibrium levels, i.e., 18% lethal and 9% male-sterile chromosomes. Two other cage popul...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:The transmission of transposed Ac elements in progeny derived by self-pollination of ten transformed tomato plants has been examined by Southern hybridization analysis. We show that six of these primary transformants have transmitted a transposed Ac to at least one progeny. One of the families was segregating for at l...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Significant correlations between allelic frequencies and environmental variables in a number of insect species have been demonstrated by multivariate techniques. Since many environmental variables show a strong relationship to geographic location and since gene flow between populations can also produce patterns of gen...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-05-01 00:00:00
abstract：:Several multilocus models that incorporate both preferential mating and viability selection are studied. Specifically, a class of symmetric heterozygosity models are considered that assign individuals to phenotypic classes according to which loci are in heterozygous state regardless of the actual allelic content. Othe...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:A series of clear mutants of the temperate phage 16-3 of Rhizobium meliloti were isolated that included various point and deletion mutants of the C cistron, coding for the phage repressor. It was observed that recombinant genotypes, such as c(+) and ti (temperature-sensitive allele), which form turbid plaques, can be ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-02-01 00:00:00
