Abstract:
:A series of clear mutants of the temperate phage 16-3 of Rhizobium meliloti were isolated that included various point and deletion mutants of the C cistron, coding for the phage repressor. It was observed that recombinant genotypes, such as c(+) and ti (temperature-sensitive allele), which form turbid plaques, can be detected quantitatively as lysogenic colonies and scored even at frequencies as low as 10(-6). Point mutations, deletions and the autonomy of intracistronic second-site mutations were characterized by this method. Further analysis has shown that each possible pair from three ti mutants gave nonconditional clear recombinants. It was shown that these latter bear the two initial ti mutations, suggesting a cumulative effect of two conditional mutations on the structure of the repressor protein. The double mutants were utilized in fine-structure mapping of the C cistron.
journal_name
Geneticsjournal_title
Geneticsauthors
Orosz Lsubject
Has Abstractpub_date
1980-02-01 00:00:00pages
265-76issue
2eissn
0016-6731issn
1943-2631journal_volume
94pub_type
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