Abstract:
:The melanosomal protein TRP2 expressed by melanocytes and most melanoma cells is an attractive, clinically relevant model antigen for the experimental development of melanoma immunotherapy in mice. A peptide shared by murine and human TRP2 can be recognized by melanoma-reactive CTL in C57BL/6 mice, as well as in human melanoma patients. Previous experiments demonstrated that gene gun immunization of mice with plasmid DNA encoding autologous murine TRP2 was unable to induce protective immunity against B16 melanoma cells naturally expressing TRP2. In the present study, we investigated whether the use of cDNA encoding xenogeneic human TRP2, which is highly homologous to murine TRP2, would be more effective. Genetic immunization of mice with human TRP2 resulted in coat depigmentation as a sign of autoimmune-mediated destruction of melanocytes and provided significant protection against metastatic growth of B16 melanoma Induction of protective immunity was associated with TRP2-reactive antibodies and CD8+ T cells. Furthermore, immunization with recombinant adenovirus was more effective than immunization with plasmid DNA using the gene gun. Our results provide new insights for the development of antigen-specific immunotherapy of melanoma.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Steitz J,Brück J,Steinbrink K,Enk A,Knop J,Tüting Tdoi
10.1002/(sici)1097-0215(20000401)86:1<89::aid-ijc1keywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
89-94issue
1eissn
0020-7136issn
1097-0215pii
10.1002/(SICI)1097-0215(20000401)86:1<89::AID-IJC1journal_volume
86pub_type
杂志文章abstract::There is increasing use of multiple molecular markers to predict prognosis in human cancer. Our aim was to examine the prognostic significance of cyclin D1 and retinoblastoma (pRb) expression in association with human papillomavirus (HPV) status in oropharyngeal squamous cell carcinoma. Clinical records and specimens ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25479
更新日期:2011-04-01 00:00:00
abstract::Cells were isolated from post-radiation fibrosis biopsies of patients with recurrent breast carcinoma. These cells were identified as fibroblasts and compared with fibroblasts from normal breast tissues for their proliferative activities, chromosome number and for the presence of various components of the extracellula...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910470512
更新日期:1991-03-12 00:00:00
abstract::Pathological deregulation of the transforming growth factor, beta 1 (TGFB1) pathway has been implicated in the development of several major diseases, including cancers. Regulatory variation in the TGFB1 gene may lead to altered TGFB1 expression and activity, and thus, modulate an individual's susceptibility to disease...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24526
更新日期:2009-09-15 00:00:00
abstract::The MLN64 gene, which is localized in q12-q21 of the human chromosome 17, encodes a novel protein containing 2 distinct domains. At the N-terminal, MLN64 exhibits a potential trans-membrane region, while at the C-terminal, it shares homology with the F26F4.4 protein of Coenorhabditis elegans and the steroidogenic acut...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970410)71:2<183::aid-ijc
更新日期:1997-04-10 00:00:00
abstract::Selenium is a promising chemopreventive agent for prostate cancer, possibly via an induction of apoptosis. Earlier studies have shown that selenite induces DNA single strand breaks (SSBs), reactive oxygen species (ROS), p53 Ser-15 phosphorylation and caspase-dependent and -independent apoptosis, whereas a methylseleno...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22480
更新日期:2007-05-01 00:00:00
abstract::In a hospital-based case-control study of esophageal cancer undertaken in Athens (1989-1991), 43 patients with incident esophageal squamous-cell carcinoma and 56 patients with incident esophageal adenocarcinoma were compared to 200 injury patients. Personal interviews were conducted in the hospital setting, and dietar...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19961104)68:3<300::AID-IJC
更新日期:1996-11-04 00:00:00
abstract::KLF5 is a transcription factor that plays important roles in multiple physical and pathological processes, including cell growth, cell cycle regulation, and angiogenesis. To better characterize KLF5 function in bladder carcinogenesis, we established stable TSU-Pr1 cell clones expressing different levels of KLF5. These...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21533
更新日期:2006-03-15 00:00:00
abstract::A new non peptidic farnesyltransferase inhibitor, RPR-115135, in combination with 5-FU was studied in 10 human colon cancer cell lines (HCT-116, RKO, DLD-1, Colo-320, LoVo, SW-620, HT-29, HCT-15, Colo-205 and KM-12) carrying several mutations but well characterized for p53 and Ras status. We found that there was a sli...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10461
更新日期:2002-07-20 00:00:00
abstract::During 1984-88 a population-based case-control study was carried out in The Netherlands in collaboration with the International Agency for Research on Cancer in order to further explore the possible relationship between diet and exocrine pancreatic carcinoma. Past habitual dietary intake was assessed in 164 cases and ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910460319
更新日期:1990-09-15 00:00:00
abstract::The role of alcoholic beverages in the etiology of gastric cancer is unclear. Recent summaries showed a positive association between higher alcohol intake and gastric cancer risk, but the magnitude of association is small, there is moderate heterogeneity among studies, and most cases were from Asian populations. We pr...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31740
更新日期:2018-12-01 00:00:00
abstract::The 1100delC mutation of the cell cycle checkpoint kinase 2 (CHEK2) gene confers an increased risk for breast cancer, but the clinical impact of other CHEK2 gene variants remains unclear. We determined the frequency of two functionally relevant CHEK2 gene mutations, I157T and IVS2+1G > A, in two large series of breast...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21022
更新日期:2005-08-20 00:00:00
abstract::The pharmacokinetics of methotrexate (MX) and 7-OH methotrexate (MXOH) was studied in 18 cancer patients treated with 6-hr intravenous (i.v.) infusion of 100 mg/kg (A), 80 mg/kg preceded by 20 mg/kg i.v. loading dose (B), and 70 mg/kg preceded by 30 mg/kg i.v. loading dose (C). Simultaneous analysis of MX and MXOH was...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910380603
更新日期:1986-12-15 00:00:00
abstract::Expression of intermediate filaments (IFs) has been suggested to be a reliable marker for differentiating epithelial and non-epithelial tumors. Moreover, the c-erbB-2 and p53 genes are considered to be involved relatively early in the process of human carcinogenesis. In order to elucidate the origin of uterine carcino...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970904)72:5<821::aid-ijc
更新日期:1997-09-04 00:00:00
abstract::The aim of this study was to identify pathways that have a significant impact during renal carcinogenesis. Sixty-seven paired samples of both noncancerous renal cortex tissue and cancerous tissue from patients with clear cell renal cell carcinomas (RCCs) were subjected to whole-exome, methylome and transcriptome analy...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28768
更新日期:2014-09-15 00:00:00
abstract::The female inflorescences of the hop plant (Humulus lupulus L.) are essential during brewing to add taste and flavor to beer and to stabilize beer foam. Xanthohumol, the main prenylated chalcone in hops, was investigated for its antiinvasive activity on human breast cancer cell lines (MCF-7 and T47-D) in vitro. Xantho...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21249
更新日期:2005-12-20 00:00:00
abstract::The stroma of ductal infiltrating carcinoma of the human breast shows characteristic and localized areas of collagen rarefaction and fragmentation. This finding has been correlated with a peculiar type of fibrillar damage, observed in a small percentage of collagen fibrils isolated in the native state from the tumour ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390510
更新日期:1987-05-15 00:00:00
abstract::More than 90% of oral cancers are histopathologically squamous cell carcinomas (SCCs). According to clinical behavior and histopathological features, we hypothesize that oral SCC can originate from either oral squamous epithelium or minor salivary glands. Here, we examined whether some oral SCCs originate from minor s...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31811
更新日期:2018-11-15 00:00:00
abstract::Variability in the expression of enzymes metabolizing carcinogens derived from cigarette smoke may contribute to individual susceptibility to pulmonary carcinogenesis. This study was designed to determine the effects of smoking and 3 major cytochrome P450 (CYP) enzymes, i.e., CYP1A1, CYP1B1 and CYP3A, which metabolize...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000601)86:5<610::aid-ijc
更新日期:2000-06-01 00:00:00
abstract::Pancreatic tumors overexpress FGF-2 and t-PA, but the implication of the growth factor in t-PA synthesis and t-PA-dependent tumor invasion remains unknown. FGF-2 is present in different isoforms: The 18 kDa FGF-2 is secreted, while the 22.5 kDa one is nuclearized and exerts intracrine regulations bypassing cell-surfac...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000215)85:4<555::aid-ijc
更新日期:2000-02-15 00:00:00
abstract::Fibrin deposition in the microenvironment of tumor cells may be critical to tumor growth and metastasis formation. Procoagulant activities of tumor cells themselves, or of infiltrating macrophages, activated by the host's immune system to the tumor, may both contribute to coagulopathies associated with metastases. Lym...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910370506
更新日期:1986-05-15 00:00:00
abstract::An important feature of cytochrome P450 (CYP) 2B1 is its high ability to convert the prodrug cyclophosphamide (CPA) to therapeutically cytotoxic metabolites, resulting in interstrand DNA-cross-linking and cell death. We have examined whether and how the phosphorylation of CYP2B1 influences CPA metabolic activation in ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1517
更新日期:2001-12-01 00:00:00
abstract::Interest has increased in the potential role of circulating tumour cells in cancer management. Most cell-based studies have been designed to determine the number of circulating tumour cells in a given volume of blood. Ability to understand the biology of the cancer cells would increase the clinical potential. The purp...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29680
更新日期:2016-01-01 00:00:00
abstract::Germline CDKN2A mutations are found in 5-20% of melanoma families. Numerous studies have shown that carriers of CDKN2A mutations have increased risks of non-melanoma cancers, but so far there have been no studies investigating cancer risks in CDKN2A wild type (wt) melanoma families. In this prospective cohort study, i...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29595
更新日期:2015-11-01 00:00:00
abstract::Cell kinetic studies are of interest for clarifying the concepts of chemotherapeutic strategy in the multimodality therapy of advanced squamous-cell carcinoma of the head and neck. A poorly-differentiated squamous-cell carcinoma of the head and neck heterotransplanted to nude mice was used for analyses of chemotherape...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910330209
更新日期:1984-02-15 00:00:00
abstract::Retinoids are a group of vitamin A analogues that have shown promise as chemopreventive and therapeutic agents in many types of malignancy and have been entered in clinical trials with some successful results. To better understand the mechanism that mediates retinoid action and the anti-proliferative effects, we treat...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970117)70:2<194::aid-ijc
更新日期:1997-01-17 00:00:00
abstract::The antigen recognized by the MOv18 MAb (Ca-MOv18) was recently shown to be a glycosylphosphatidylinositol (GPI)-linked protein. In this report we show that GPI-anchorage is not limited to IGROVI cells nor to other ovary carcinoma cell lines, but Ca-MOv18 was also found to be sensitive to phosphatidylinositol-specific...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910510326
更新日期:1992-05-28 00:00:00
abstract::In a novel attempt to understand the variations in DNA sequences underlying HLA class I alleles associated with HPV16-related CaCx, we determined the alleles by reconstructing SNP-based haplotypes from resequencing of the most polymorphic exons 2 and 3 of HLA-A, HLA-B and HLA-C. We also determined the impact of SNPs a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30681
更新日期:2017-06-01 00:00:00
abstract::Condylomata acuminata have been shown to increase the risk of anogenital cancers. However, previous studies have been of limited sample size and/or short follow-up duration, which prevent precise estimates of long-term excess risk, especially for specific cancer sites. We estimated the risk of specific cancers in a la...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21892
更新日期:2006-08-15 00:00:00
abstract::We have studied the production and release of cholecystokinin (CCK) forms by rat medullary thyroid cancer (MTC) in vivo. MTC cells were inoculated s.c. into 8 Wag-Rij rats. One month later, after i.v. injection of calcium plasma levels of CCK, calcitonin and tissue contents of gastrin and calcitonin were determined by...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910480510
更新日期:1991-07-09 00:00:00
abstract::The effect of blockade of NF-kappaB activity on human pancreatic cancer angiogenesis was determined in an orthotopic xenograft model. Highly metastatic L3.3 human pancreatic cancer cells, which expressed an elevated level of constitutive NF-kappaB activity, were transfected with a mutated IkappaBalpha (IkappaBalphaM)....
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11562
更新日期:2004-01-10 00:00:00