Membrane association and shedding of the GPI-anchored Ca-MOv18 antigen in human ovary carcinoma cells.


:The antigen recognized by the MOv18 MAb (Ca-MOv18) was recently shown to be a glycosylphosphatidylinositol (GPI)-linked protein. In this report we show that GPI-anchorage is not limited to IGROVI cells nor to other ovary carcinoma cell lines, but Ca-MOv18 was also found to be sensitive to phosphatidylinositol-specific phospholipase C (PI-PLC) treatment on fresh ovarian cancer cells. Furthermore, we found a heterogeneous sensitivity of Ca-MOv18 to PI-PLC cleavage, not only among the different cells studied but also in different experiments performed on the same cell line, during extended periods of time in culture. Sensitivity to PI-PLC cleavage was determined by immunofluorescence on live cells and by double-determinant radioimmunoassay of the antigen released in the supernatant. The specificity of the PI-PLC cleavage was demonstrated as follows: (a) TX114 solubilized Ca-MOv18 shifts from the detergent to the aqueous phase after treatment with PI-PLC; (b) on membrane preparations, PI-PLC specifically released a fraction of the antigen, which is distinct from the weakly associated form released by high-salt treatment; (c) Ca-MOv18 from IGROVI expressed the cross-reacting determinant (CRD), which is characteristic of GPI-linked molecules. The absence of CRD expression on the spontaneously released protein and the possibility of artificially inducing antigen shedding during the resynthesis of Ca-MOv18 which follows bacterial PI-PLC treatment are interesting points which need to be further investigated in order to understand the physiology of the Ca-MOv18 tumor antigen.


Int J Cancer


Miotti S,Alberti S,Facheris P,Mantovani L,Fornaro M,Stella M,Ménard S,Canevari S,Colnaghi MI





Has Abstract


1992-05-28 00:00:00












  • Prevention of benzo(a)pyrene-induced mutagenicity by homogeneous epoxide hydratase.

    abstract::Benzo(a)pyrene and benz(a) anthrancene which, in contrast to the K-region epoxides benzo(a)pyrene 4,5-oxide and benz(a)anthracene 5,6-oxide, are not mutagenic to Salmonella typhimurium TA 1537 in the absence of mammalian enzyme preparations, were activated by liver microsomes from C3H mice, which had not received any ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Oesch F,Bentley P,Glatt HR

    更新日期:1976-10-15 00:00:00

  • Role of natural and adaptive immunity in renal cell carcinoma response to VEGFR-TKIs and mTOR inhibitor.

    abstract::Angiogenesis and immunosuppression work hand-in-hand in the renal cell carcinoma (RCC) microenvironment. Tumor growth is associated with impaired antitumor immune response in RCC, which involves T cells, natural killer cells, dendritic cells (DCs) and macrophages. Vascular endothelial growth factor receptor (VEGFR), s...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Santoni M,Berardi R,Amantini C,Burattini L,Santini D,Santoni G,Cascinu S

    更新日期:2014-06-15 00:00:00

  • Transcriptional regulation of urokinase (uPA) gene expression in breast cancer cells: role of DNA methylation.

    abstract::Carcinoma of the breast is a leading hormone-dependent malignancy, resulting in a high rate of morbidity and mortality. During the complex multi-step process of tumor promotion, this common cancer is initiated as hormone-responsive (HR), non-metastatic cancer, followed by a gradual transition into a highly metastatic ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Xing RH,Rabbani SA

    更新日期:1999-05-05 00:00:00

  • Risk of second cancer after lymphohematopoietic neoplasm.

    abstract::People living with lymphohematopoietic neoplasms (LHNs) are known to have increased risks of second cancer; however, the incidence of second cancers after LHNs has not been studied extensively in Australia. The Australian Cancer Database was used to analyze site-specific risk of second primary cancer after LHNs in 127...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Royle JS,Baade P,Joske D,Fritschi L

    更新日期:2011-08-15 00:00:00

  • Cervical cancer cell-derived interleukin-6 impairs CCR7-dependent migration of MMP-9-expressing dendritic cells.

    abstract::Cervical carcinogenesis is a consequence of persistent infection with high-risk human papillomaviruses (HPVs). Recent studies indicate that HPV-transformed cells actively instruct their microenvironment to promote carcinogenesis. Here, we demonstrate that cervical cancer cells activate monocytes to produce their own C...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pahne-Zeppenfeld J,Schröer N,Walch-Rückheim B,Oldak M,Gorter A,Hegde S,Smola S

    更新日期:2014-05-01 00:00:00

  • Unexpected paracrine action of prostate cancer cells harboring a new class of androgen receptor mutation--a new paradigm for cooperation among prostate tumor cells.

    abstract::The emergence of mutations in the androgen receptor (AR) gene is a recurrent event during progression of prostate cancer (PCa) on androgen ablation therapy. In this study, we show that nonsense mutations that lead to carboxyl-terminal end truncated ARs are found at high frequency in metastatic PCas. Transcriptional ac...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lapouge G,Erdmann E,Marcias G,Jagla M,Monge A,Kessler P,Serra S,Lang H,Jacqmin D,Bergerat JP,Céraline J

    更新日期:2007-09-15 00:00:00

  • Trends in childhood acute lymphoblastic leukemia in Western Australia, 1960-2006.

    abstract::Increases in the incidence of childhood acute lymphoblastic leukemia (ALL) have been reported in some countries, while other reports from similar geographical regions have indicated stable rates. The reasons for the discrepancies have been debated in the literature, with the focus on whether the observed increases are...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Milne E,Laurvick CL,de Klerk N,Robertson L,Thompson JR,Bower C

    更新日期:2008-03-01 00:00:00

  • A functional TNFRSF5 polymorphism and risk of non-Hodgkin lymphoma, a pooled analysis.

    abstract::Interaction between CD40 and its ligand, CD154, has a key function in immune regulation. Recent experimental data support a role of deregulated CD40 signalling in lymphomagenesis. Data from earlier studies that are part of this pooling study implicate a functional polymorphism (-1C>T, rs1883832) in the TNFRSF5 gene en...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Nieters A,Bracci PM,de Sanjosé S,Becker N,Maynadié M,Benavente Y,Foretova L,Cocco P,Staines A,Holly EA,Boffetta P,Brennan P,Skibola CF

    更新日期:2011-03-15 00:00:00

  • Fifty years of cancer incidence: CI5 I-IX.

    abstract::The Cancer Incidence in Five Continents (CI5) series comprises nine volumes that bring together peer-reviewed results from population-based cancer registries worldwide. The aim of each is to make available comparable data on cancer incidence from as wide a range of geographical locations as possible. In addition, the ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Parkin DM,Ferlay J,Curado MP,Bray F,Edwards B,Shin HR,Forman D

    更新日期:2010-12-15 00:00:00

  • Biological effects induced by insulin-like growth factor binding protein 3 (IGFBP-3) in malignant melanoma.

    abstract::The insulin like growth factor (IGF) signaling pathway has been shown to contribute to melanoma progression, but little is known about the role of the IGF binding protein 3 (IGFBP-3) in melanoma biology. The aim of the present study was to characterize expression, function and regulation of IGFBP-3 in malignant melano...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Oy GF,Slipicevic A,Davidson B,Solberg Faye R,Maelandsmo GM,Flørenes VA

    更新日期:2010-01-15 00:00:00

  • Effects of an inducible anti-sense c-myc gene transfer in a drug-resistant human small-cell-lung-carcinoma cell line.

    abstract::Small-cell-lung-cancer (SCLC) is characterized by rapid development of resistance to cytotoxic agents, such as cisplatin (cDDP) and anthracyclines. c-myc over-expression is one of the reported genetic alterations in this tumor. Amplification of the c-myc gene in this and other cancers is often correlated with poor pro...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Van Waardenburg RC,Meijer C,Burger H,Nooter K,De Vries EG,Mulder NH,De Jong S

    更新日期:1997-11-14 00:00:00

  • Effects of the farnesyltransferase inhibitor UCF-1C/manumycin on growth and p21-ras post-translational processing in NIH3T3 cells.

    abstract::Examination of the effect of the farnesylprotein transferase (FPTase) inhibitor UCF1-C/manumycin on NIH3T3 cells transfected with a normal N-ras gene and expressing high levels of the corresponding p21-ras protein showed that 10 microm UCF1-C immediately and reversibly inhibited growth in these cells, without modifyin...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Servais P,Gulbis B,Fokan D,Galand P

    更新日期:1998-05-18 00:00:00

  • Pharmacokinetics of methotrexate and its 7-OH metabolite in cancer patients treated with different high-methotrexate dosage regimens.

    abstract::The pharmacokinetics of methotrexate (MX) and 7-OH methotrexate (MXOH) was studied in 18 cancer patients treated with 6-hr intravenous (i.v.) infusion of 100 mg/kg (A), 80 mg/kg preceded by 20 mg/kg i.v. loading dose (B), and 70 mg/kg preceded by 30 mg/kg i.v. loading dose (C). Simultaneous analysis of MX and MXOH was...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: el-Yazigi A,Amer M,Al-Saleh I,Martin C

    更新日期:1986-12-15 00:00:00

  • Inhibition of experimental pulmonary metastasis by controlling biodistribution of catalase in mice.

    abstract::In a previous study, we showed that targeted delivery of bovine liver catalase to hepatocytes by direct galactosylation augmented the inhibitory effect of the enzyme on experimental hepatic metastasis of colon carcinoma cells (unpublished data). Here, we examined the ability of catalase to inhibit tumor metastasis to ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Nishikawa M,Tamada A,Kumai H,Yamashita F,Hashida M

    更新日期:2002-05-20 00:00:00

  • Expression of MAGE genes in transitional-cell carcinomas of the urinary bladder.

    abstract::Human genes MAGE-1 and MAGE-3 code for distinct antigens, which are recognized on melanoma cells by autologous cytolytic T lymphocytes (CTL). These antigens may constitute useful targets for anti-cancer immunotherapy, since no expression of MAGE genes has been observed in normal tissues other than testis. Out of 57 sa...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Patard JJ,Brasseur F,Gil-Diez S,Radvanyi F,Marchand M,François P,Abi-Aad A,Van Cangh P,Abbou CC,Chopin D

    更新日期:1995-02-20 00:00:00

  • Second primary tumors in patients with hereditary retinoblastoma: a register-based follow-up study, 1945-1994.

    abstract::The aim of this register-based follow-up study was to evaluate the long-term cumulative incidence of second primary tumors (SPT) among survivors of hereditary retinoblastoma, with special interest for the incidence of pineoblastoma in retinoblastoma patients born after 1970. The Dutch Retinoblastoma Register was compl...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Moll AC,Imhof SM,Bouter LM,Kuik DJ,Den Otter W,Bezemer PD,Koten JW,Tan KE

    更新日期:1996-08-07 00:00:00

  • Modulation of invasive properties of murine squamous carcinoma cells by heterologous expression of cathepsin B and cystatin C.

    abstract::Murine SCC-VII squamous carcinoma cells have the capacity to penetrate reconstituted basement membranes (Matrigel) in vitro. The invasion of Matrigel layers by SCC-VII cells was significantly reduced by E-64, a specific inhibitor of lysosomal cysteine proteinases. The cathepsin-B-selective E-64 derivative, CA-074, inh...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Coulibaly S,Schwihla H,Abrahamson M,Albini A,Cerni C,Clark JL,Ng KM,Katunuma N,Schlappack O,Glössl J,Mach L

    更新日期:1999-11-12 00:00:00

  • 6-thioguanine resistance in a human colon carcinoma cell line with unaltered levels of hypoxanthine guanine phosphoribosyltransferase activity.

    abstract::Cell populations resistant to high doses (30 microM) of 6-thioguanine (6-TG, 6-TG(r) cells) were selected from a human colon carcinoma cell line, LoVo. This cell line, which lacks hMSH2, a component of the human mismatch binding heterodimer hMutSalpha, is resistant to low doses of 6-TG. The level of activity of hypoxa...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bemi V,Turchi G,Margotti E,Giorgelli F,Pesi R,Sgarrella F,Tozzi MG,Camici M

    更新日期:1999-08-12 00:00:00

  • The expression of hematopoietic progenitor cell antigen CD34 is regulated by DNA methylation in a site-dependent manner in gastrointestinal stromal tumours.

    abstract::The anatomic site-dependent expression of hematopoietic progenitor cell antigen CD34 is a feature of gastrointestinal stromal tumours (GISTs). The basis for the differential CD34 expression is only incompletely understood. This study aimed at understanding the regulation of CD34 in GISTs and clarification of its site-...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bure I,Braun A,Kayser C,Geddert H,Schaefer IM,Cameron S,Ghadimi MB,Ströbel P,Werner M,Hartmann A,Wiemann S,Agaimy A,Haller F,Moskalev EA

    更新日期:2017-12-01 00:00:00

  • p16/MTS1 inactivation in ovarian carcinomas: high frequency of reduced protein expression associated with hyper-methylation or mutation in endometrioid and mucinous tumors.

    abstract::Inactivation of the tumor-suppressor gene p16 (MTS1/ CDKN2/INK4a) has been described in various human malignancies. Although p16 deletion has been found in various ovarian tumor cell lines, p16 inactivation by homozygous deletion or mutation has been reported only sporadically in primary ovarian carcinomas. In a compr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Milde-Langosch K,Ocon E,Becker G,Löning T

    更新日期:1998-02-20 00:00:00

  • Site distribution of cutaneous melanoma in Queensland.

    abstract::The age-adjusted incidence rates of cutaneous melanoma in Queensland in 1987 have been analysed for 16 anatomic sites, taking into account their surface areas. In men, the incidence of invasive melanoma on the ears, a chronically sun-exposed site, was extraordinarily high with annual rates of over 200 per 10(5) units ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Green A,MacLennan R,Youl P,Martin N

    更新日期:1993-01-21 00:00:00

  • The traditional Chinese herbal compound rocaglamide preferentially induces apoptosis in leukemia cells by modulation of mitogen-activated protein kinase activities.

    abstract::With an increasing cancer rate worldwide, there is an urgent quest for the improvement of anticancer drugs. One of the main problems of present chemotherapy in treatment of tumor patients is the toxicity of drugs. Most of the existent anticancer drugs, unfortunately, attack also proliferating normal cells. In recent y...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Zhu JY,Lavrik IN,Mahlknecht U,Giaisi M,Proksch P,Krammer PH,Li-Weber M

    更新日期:2007-10-15 00:00:00

  • Differential lysis of melanoma clones by autologous recombinant interleukin 2-activated lymphocytes. Relationship with spontaneous resistance to doxorubicin (Dx).

    abstract::To investigate whether human melanoma cells intrinsically resistant to autologous LAKs do exist, and whether a relationship between the level of lysis of LAKs and spontaneous drug resistance can be identified at the clonal level, we studied 44 clones obtained from a metastatic melanoma lesion. The antigenic phenotype ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gambacorti-Passerini C,Rivoltini L,Supino R,Mariani M,Parmiani G

    更新日期:1988-10-15 00:00:00

  • Involvement of nucleophosmin/B23 in the response of HeLa cells to UV irradiation.

    abstract::The steady-state mRNA level of nucleophosmin/B23 in HeLa cells increased after UV irradiation. Nucleophosmin/B23 antisense transfection potentiated ultraviolet (UV)-induced cell killing. A block in G(2)/M phase, larger peak of apoptotic cells and higher caspase-3 in vitro activity were noted in nucleophosmin/B23 antis...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wu MH,Chang JH,Chou CC,Yung BY

    更新日期:2002-01-20 00:00:00

  • Involvement of p53 in specific anti-neuroectodermal tumor activity of aloe-emodin.

    abstract::Previously, we have identified aloe-emodin (AE) as a new type of anticancer agent, with activity that is based on apoptotic cell death promoted by a neuroectodermal tumor-specific drug uptake. We attempt to clarify the intracellular target of AE and the apoptosis-signaling pathway activated by AE in neuroblastoma cell...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Pecere T,Sarinella F,Salata C,Gatto B,Bet A,Dalla Vecchia F,Diaspro A,Carli M,Palumbo M,Palù G

    更新日期:2003-10-10 00:00:00

  • Time to clearance of human papillomavirus infection by type and human immunodeficiency virus serostatus.

    abstract::Persistent infection with high-risk human papillomavirus (HPV) is central to cervical carcinogenesis. Certain high-risk types, such as HPV16, may be more persistent than other HPV types, and type-specific HPV persistence may differ by HIV serostatus. This study evaluated the association between HPV type and clearance ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Koshiol JE,Schroeder JC,Jamieson DJ,Marshall SW,Duerr A,Heilig CM,Shah KV,Klein RS,Cu-Uvin S,Schuman P,Celentano D,Smith JS

    更新日期:2006-10-01 00:00:00

  • The B cell antigen receptor in atypical chronic lymphocytic leukemia with t(14;19)(q32;q13) demonstrates remarkable stereotypy.

    abstract::The t(14;19)(q32;q13) is a recurrent chromosomal translocation reported in a variety of B-cell leukemias and lymphomas, including chronic lymphocytic leukemia (CLL). CLL cases associated with t(14;19) often have atypical morphologic and immunophenotypic features and unmutated immunoglobulin heavy chain (IGH) variable ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Schweighofer CD,Huh YO,Luthra R,Sargent RL,Ketterling RP,Knudson RA,Barron LL,Medeiros LJ,Keating MJ,Abruzzo LV

    更新日期:2011-06-01 00:00:00

  • An eHealth decision-support tool to prioritize referral practices for genetic evaluation of patients with Wilms tumor.

    abstract::Over 10% of children with Wilms tumor (WT) have an underlying cancer predisposition syndrome (CPS). Cognizant of increasing demand for genetic evaluation and limited resources across health care settings, there is an urgent need to rationalize genetic referrals for this population. The McGill Interactive Pediatric Onc...

    journal_title:International journal of cancer

    pub_type: 杂志文章,多中心研究


    authors: Cullinan N,Villani A,Mourad S,Somers GR,Reichman L,van Engelen K,Stephens D,Weksberg R,Foulkes WD,Malkin D,Grant R,Goudie C

    更新日期:2020-02-15 00:00:00

  • Hot maté drinking and precancerous lesions of the oesophagus: an endoscopic survey in southern Brazil.

    abstract::Thermal injury resulting from drinking very hot beverages has been incriminated as a risk factor for oesophageal cancer, although no information is available on the lesions caused by this injury in human or experimental animals. The drinking of hot maté tea is very common in areas of moderately high incidence of oesop...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Muñoz N,Victora CG,Crespi M,Saul C,Braga NM,Correa P

    更新日期:1987-06-15 00:00:00

  • A mutant KRAS-induced factor REG4 promotes cancer stem cell properties via Wnt/β-catenin signaling.

    abstract::Mutant KRAS provides a driving force for enhancement of cancer stem cells (CSCs) characteristics contributing transformation of colorectal cancer (CRC) cells harboring adenomatous polyposis coli (APC) mutations. Here, we identified the factors mediating the promotion of CSCs properties induced by KRAS mutation through...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hwang JH,Yoon J,Cho YH,Cha PH,Park JC,Choi KY

    更新日期:2020-05-15 00:00:00