Abstract:
:TFF-peptides (formerly P-domain peptides, trefoil factors) are typical secretory products of mucin-producing cells and seem to influence the rheological properties of mucous gels. Here, localization studies of TFF-peptides in human salivary glands are presented. Expression studies (polymerase chain reaction) revealed mainly TFF3 transcripts in submandibular and sublingual glands and trace amounts in parotid glands. Only low levels of expression of TFF1 could be monitored in submandibular and sublingual glands, and TFF2 transcripts were hardly detectable in all three major salivary glands. This result was partly confirmed by Western blot analysis, which only detected TFF3 in submandibular glands, but not in sublingual and parotid glands. TFF3 was also shown to be a constituent of human saliva. Immunofluorescence localized TFF3 solely in the secretory granules of serous cells of submandibular glands but not in mucous cells. This localization is remarkably similar to that of the unique low-molecular-weight mucin MUC7, which interacts with a number of oral microorganisms.
journal_name
Cell Tissue Resjournal_title
Cell and tissue researchauthors
Jagla W,Wiede A,Hinz M,Dietzmann K,Gülicher D,Gerlach KL,Hoffmann Wdoi
10.1007/s004419900087keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
161-6issue
1eissn
0302-766Xissn
1432-0878journal_volume
298pub_type
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