Secretion of TFF-peptides by human salivary glands.

Abstract:

:TFF-peptides (formerly P-domain peptides, trefoil factors) are typical secretory products of mucin-producing cells and seem to influence the rheological properties of mucous gels. Here, localization studies of TFF-peptides in human salivary glands are presented. Expression studies (polymerase chain reaction) revealed mainly TFF3 transcripts in submandibular and sublingual glands and trace amounts in parotid glands. Only low levels of expression of TFF1 could be monitored in submandibular and sublingual glands, and TFF2 transcripts were hardly detectable in all three major salivary glands. This result was partly confirmed by Western blot analysis, which only detected TFF3 in submandibular glands, but not in sublingual and parotid glands. TFF3 was also shown to be a constituent of human saliva. Immunofluorescence localized TFF3 solely in the secretory granules of serous cells of submandibular glands but not in mucous cells. This localization is remarkably similar to that of the unique low-molecular-weight mucin MUC7, which interacts with a number of oral microorganisms.

journal_name

Cell Tissue Res

journal_title

Cell and tissue research

authors

Jagla W,Wiede A,Hinz M,Dietzmann K,Gülicher D,Gerlach KL,Hoffmann W

doi

10.1007/s004419900087

keywords:

subject

Has Abstract

pub_date

1999-10-01 00:00:00

pages

161-6

issue

1

eissn

0302-766X

issn

1432-0878

journal_volume

298

pub_type

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