Abstract：
:Microspheres comprised of particulate hydroxyapatites dispersed in fibrous collagen matrices were prepared. The procedure involved the droplet formation of hydroxyapatite/collagen mixture emulsified in olive oil, followed by the reconstitution of collagen in the presence of hydroxyapatite particles at 37 degrees C. Various sizes of microspheres could be obtained by controlling the stirring speed of the emulsified mixture. By increasing the stirring speed from 200 to 350 and 500 rpm, the average diameter of the microspheres decreased from 1038 to 513 and 226 microm, respectively. The sizes of the microspheres reduced substantially to a range of 141 microm when 2%. Span 85 was present in the emulsion mixture stirring at 200 rpm. The microspheres thus obtained can be used as carriers to support the growth of osteoblast cells. Osteoblast cells derived from calvaria proliferated from 1.5 x 10(5) to 4.5 x 10(5) cells/ml in 7 days. Correspondingly, the alkaline phosphatase activity increased 6 fold during this period. These results suggested that the hydroxyapatite/collagen microspheres could be used as the filling materials for bone defect.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Hsu FY,Chueh SC,Wang YJdoi
10.1016/s0142-9612(99)00095-2keywords:
subject
Has Abstractpub_date
1999-10-01 00:00:00pages
1931-6issue
20eissn
0142-9612issn
1878-5905pii
S0142961299000952journal_volume
20pub_type
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