Protease-degradable microgels for protein delivery for vascularization.

Abstract:

:Degradable hydrogels to deliver bioactive proteins represent an emerging platform for promoting tissue repair and vascularization in various applications. However, implanting these biomaterials requires invasive surgery, which is associated with complications such as inflammation, scarring, and infection. To address these shortcomings, we applied microfluidics-based polymerization to engineer injectable poly(ethylene glycol) microgels of defined size and crosslinked with a protease degradable peptide to allow for triggered release of proteins. The release rate of proteins covalently tethered within the microgel network was tuned by modifying the ratio of degradable to non-degradable crosslinkers, and the released proteins retained full bioactivity. Microgels injected into the dorsum of mice were maintained in the subcutaneous space and degraded within 2 weeks in response to local proteases. Furthermore, controlled release of VEGF from degradable microgels promoted increased vascularization compared to empty microgels or bolus injection of VEGF. Collectively, this study motivates the use of microgels as a viable method for controlled protein delivery in regenerative medicine applications.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Foster GA,Headen DM,González-García C,Salmerón-Sánchez M,Shirwan H,García AJ

doi

10.1016/j.biomaterials.2016.10.044

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

170-175

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(16)30595-6

journal_volume

113

pub_type

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