Abstract:
BACKGROUND:Nonencapsulated and nontypeable Haemophilus influenzae (NTHi) is a major cause of human respiratory tract infections. Some strains of NTHi can cause invasive diseases such as septicemia and meningitis, even if H. influenzae is not generally considered to be an intracellular pathogen. There have been very few reports about the therapeutic efficacy of antibiotics against respiratory tract infection caused by NTHi in mice because it is difficult for H. influenzae to infect mice. Therefore, we evaluated the efficacy of antibiotics against NTHi in both a cell culture model and a mouse model of infection. METHODS:We used six strains of NTHi isolated from adult patients with chronic otitis media, namely three beta-lactamase-negative ampicillin (AMP)-resistant (BLNAR) strains and three beta-lactamase-negative AMP-susceptible (BLNAS) strains, to evaluate the efficacy of AMP, cefcapene (CFPN), levofloxacin (LVX), clarithromycin (CLR), and azithromycin (AZM) in both a cell culture infection model and a mouse infection model. In the cell culture infection model, strains that invade A549 human alveolar epithelial cells were treated with each antibiotic (1 mug/ml). In the mouse infection model, female C57BL/6 mice were intraperitoneally injected with cyclophosphamide (200 mg/kg) three days before intranasal infection with 1 x 109 colony-forming units (CFU) of NTHi and on the day of infection. After infection, the mice were orally administered each antibiotic three times daily for three days, except for AZM, which was administered once daily for three days, at a dose of 100 mg/kg/day. RESULTS:In the cell culture infection model, it was found that two BLNAR strains were able to enter the cell monolayers by the process of macropinocytosis, and treatment with LVX yielded good bactericidal activity against both strains inside the cells. In the mouse infection model, no bacteria were detected by means of plating the lung homogenates of LVX-treated mice at day 4 after infection, while more than 105 CFU of bacteria per tissue sample were detected in nontreated mice. CONCLUSION:Our findings show the outcome and rich benefits of fluoroquinolone treatment of respiratory infections caused by either invasive or noninvasive BLNAR strains of NTHi.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Sekiya Y,Eguchi M,Nakamura M,Ubukata K,Omura S,Matsui Hdoi
10.1186/1471-2334-8-15subject
Has Abstractpub_date
2008-02-07 00:00:00pages
15issn
1471-2334pii
1471-2334-8-15journal_volume
8pub_type
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3423-z
更新日期:2018-10-16 00:00:00
abstract:BACKGROUND:The standard of care for HCV Hepatitis is the combination of interferon (IFN) plus Ribavirin. In HIV patients the use of this combination therapy may induce drug interactions, and reduces the adherence to HAART. The aim of this study is to evaluate safety and efficacy of a 48 weeks daily dose IFN schedule. ...
journal_title:BMC infectious diseases
pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,meta分析
doi:10.1186/s12879-020-4879-1
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abstract:BACKGROUND:Although there is a large increase in investment for tuberculosis control in Myanmar, there are few operational analyses to inform policies. Only 34% of nationally reported cases are from women. In this study, we investigate sex differences in tuberculosis diagnoses in Myanmar in order to identify potential ...
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pub_type: 杂志文章
doi:10.1186/s12879-016-1429-y
更新日期:2016-03-03 00:00:00
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pub_type: 杂志文章
doi:10.1186/1471-2334-13-240
更新日期:2013-05-24 00:00:00
abstract:BACKGROUND:Spinal neuroschistosomiasis (SN) is one of the most severe clinical presentations of schistosomiasis infection and an ectopic form of the disease caused by any species of Schistosoma. In Brazil, all cases of this clinical manifestation are related to Schistosoma mansoni, the only species present in the count...
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1186/1471-2334-9-190
更新日期:2009-11-29 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12879-017-2348-2
更新日期:2017-04-11 00:00:00
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pub_type: 杂志文章
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更新日期:2020-08-27 00:00:00
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pub_type: 杂志文章
doi:10.1186/1471-2334-12-73
更新日期:2012-03-29 00:00:00
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pub_type: 杂志文章
doi:10.1186/1471-2334-4-4
更新日期:2004-02-13 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12879-019-4035-y
更新日期:2019-05-16 00:00:00
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pub_type: 杂志文章
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更新日期:2014-09-22 00:00:00
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pub_type: 杂志文章
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doi:10.1186/s12879-017-2651-y
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pub_type: 杂志文章
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更新日期:2020-02-26 00:00:00
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pub_type: 杂志文章
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更新日期:2020-12-04 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12879-019-3741-9
更新日期:2019-01-31 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12879-020-05264-4
更新日期:2020-07-25 00:00:00
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-015-1013-x
更新日期:2015-08-11 00:00:00
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-11-341
更新日期:2011-12-14 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12879-019-3815-8
更新日期:2019-02-20 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12879-018-3193-7
更新日期:2018-07-04 00:00:00