Abstract:
BACKGROUND:The impacts of genetic polymorphisms on drug resistance mutations (DRMs) among various HIV-1 subtypes have long been debated. In this study, we aimed to analyze the natural polymorphisms and acquired DRM profile in HIV-1 CRF01_AE-infected patients in a large first-line antiretroviral therapy (ART) cohort in northeastern China. METHODS:The natural polymorphisms of CRF01_AE were analyzed in 2034 patients from a long-term ART cohort in northeastern China. The polymorphisms in 105 treatment failure (TF) patients were compared with those in 1148 treatment success (TS) patients. The acquired DRM profile of 42 patients who experienced TF with tenofovir/lamivudine/efavirenz (TDF/3TC/EFV) treatment was analyzed by comparing the mutations at TF time point to those at baseline. The Stanford HIVdb algorithm was used to interpret the DRMs. Binomial distribution, McNemar test, Wilcoxon test and CorMut package were used to analyze the mutation rates and co-variation. Deep sequencing was used to analyze the evolutionary dynamics of co-variation. RESULTS:Before ART, there were significantly more natural polymorphisms of 31 sites on reverse transcriptase (RT) in CRF01_AE than subtype B HIV-1 (|Z value| ≥ 3), including five known drug resistance-associated sites (238, 118, 179, 103, and 40). However, only the polymorphism at site 75 was associated with TF (|Z value| ≥ 3). The mutation rate at 14 sites increased significantly at TF time point compared to baseline, with the most common DRMs comprising G190S/C, K65R, K101E/N/Q, M184 V/I, and V179D/I/A/T/E, ranging from 66.7 to 45.2%. Moreover, two unknown mutations (V75 L and L228R) increased by 19.0 and 11.9% respectively, and they were under positive selection (Ka/Ks > 1, log odds ratio [LOD] > 2) and were associated with several other DRMs (cKa/Ks > 1, LOD > 2). Deep sequencing of longitudinal plasma samples showed that L228R occurred simultaneously or followed the appearance of Y181C. CONCLUSION:The high levels of natural polymorphisms in CRF01_AE had little impact on treatment outcomes. The findings regarding potential new CRF01_AE-specific minor DRMs indicate the need for more studies on the drug resistance phenotype of CRF01_AE.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Sun Z,Ouyang J,Zhao B,An M,Wang L,Ding H,Han Xdoi
10.1186/s12879-020-4808-3subject
Has Abstractpub_date
2020-02-26 00:00:00pages
178issue
1issn
1471-2334pii
10.1186/s12879-020-4808-3journal_volume
20pub_type
杂志文章abstract:BACKGROUND:On 18 August 2015, Kyegegwa District reported eight deaths during a measles outbreak to the Uganda Ministry of Health (MoH). We investigated this death cluster to verify the cause, identify risk factors, and inform public health interventions. METHODS:We defined a probable measles case as onset of fever and...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2558-7
更新日期:2017-07-03 00:00:00
abstract:BACKGROUND:H7N9 posed potentially serious health challenges for Chinese society. The previous SARS outbreak in this country was accompanied by contradictory information, while worries about wide-spread influenza led to discrimination worldwide. Early understanding of public threat perceptions is therefore important for...
journal_title:BMC infectious diseases
pub_type: 杂志文章
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abstract:BACKGROUND:Infection by Staphylococcus aureus (S. aureus) is a major cause of morbidity and mortality. Colonization by S. aureus increases the risk of infection. Little is known about decolonization strategies for S. aureus beyond antibiotics, however probiotics represent a promising alternative. A randomized controlle...
journal_title:BMC infectious diseases
pub_type: 杂志文章,随机对照试验
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journal_title:BMC infectious diseases
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abstract:BACKGROUND:After antibiotic treatment of Lyme borreliosis, a subset of patients report persistent symptoms, also referred to as post-treatment Lyme disease syndrome. The reported prevalence of persistent symptoms varies considerably, and its pathophysiology is under debate. The LymeProspect study has been designed to i...
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1186/1471-2334-6-54
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-480
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3560-4
更新日期:2018-12-06 00:00:00
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章,meta分析
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更新日期:2019-06-17 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12879-019-4176-z
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pub_type: 杂志文章
doi:10.1186/s12879-016-1760-3
更新日期:2016-08-20 00:00:00
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pub_type: 杂志文章,meta分析
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更新日期:2013-01-22 00:00:00
abstract:BACKGROUND:Hospital-based surveillance for pneumococcal meningitis has been conducted since January 1996 in the city of Salvador, Brazil. The purpose of this study was to describe the temporal evolution of Penicillin Non-Susceptible Streptococcus pneumoniae (PNSSP) in regards to serotype distributions and clonal divers...
journal_title:BMC infectious diseases
pub_type: 杂志文章
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更新日期:2015-07-30 00:00:00
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-4847-9
更新日期:2020-02-11 00:00:00
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journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-4-53
更新日期:2004-11-29 00:00:00
abstract:BACKGROUND:The effects of helminth co-infection on malaria in humans remain uncertain. This study aimed to evaluate the nature of association of intestinal helminths with prevalence and clinical outcomes of Plasmodium infection. METHODS:A cross-sectional study involving 1,065 malaria suspected febrile patients was con...
journal_title:BMC infectious diseases
pub_type: 杂志文章
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更新日期:2012-11-09 00:00:00
abstract:BACKGROUND:Human parechovirus type 3 (HPeV-3) is known to cause cold-like symptoms, diarrhea, or severe infections such as sepsis in infants and children. In adults, HPeV-3 infection is rarely diagnosed because the symptoms are generally mild and self-limiting; however, this infection has been linked to epidemic myalgi...
journal_title:BMC infectious diseases
pub_type: 杂志文章,评审
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更新日期:2018-08-07 00:00:00