Abstract：
:Directed open reading frame (ORF) disruption and a serial selection technique in Escherichia coli and the extremely thermophilic archaeon Sulfolobus solfataricus allowed the identification of otherwise cryptic crucial and noncrucial viral open reading frames in the genome of the archaeal virus SSV1. It showed that the 15. 5-kbp viral genome can incorporate a 2.96-kbp insertion without loss of viral function and package this DNA properly into infectious virus particles. The selection technique, based on the preferential binding of ethidium bromide to relaxed DNA and the resulting inhibition of endonuclease cleavage to generate a pool of mostly singly cut molecules, should be generally applicable. A fully functional viral shuttle vector for S. solfataricus and E. coli was made. This vector spreads efficiently through infected cultures of S. solfataricus, its replication is induced by UV irradiation, it forms infectious virus particles, and it is stable at high copy number in both S. solfataricus and E. coli. The classification of otherwise unidentifiable ORFs in SSV1 facilitates genetic analysis of this virus, and the shuttle vector should be useful for the development of genetic systems for Crenarchaeota.
journal_name
Geneticsjournal_title
Geneticsauthors
Stedman KM,Schleper C,Rumpf E,Zillig Wkeywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
1397-405issue
4eissn
0016-6731issn
1943-2631journal_volume
152pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Aimed at investigating the recovery of a specific mutant allele of the mating type locus (MAT) by switching a defective MAT allele, these experiments provide information bearing on several models proposed for MAT interconversion in bakers yeast, Saccharomyces cerevisiae. Hybrids between heterothallic (ho) cells carryi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-07-01 00:00:00
abstract：:Centromeres control chromosome inheritance in eukaryotes, yet their DNA structure and primary sequence are hypervariable. Most animals and plants have megabases of tandem repeats at their centromeres, unlike yeast with unique centromere sequences. Centromere function requires the centromere-specific histone CENH3 (CEN...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.120337
更新日期：2010-10-01 00:00:00
abstract：:Methods for inference and interpretation of evolutionary quantitative genetic parameters, and for prediction of the response to selection, are best developed for traits with normal distributions. Many traits of evolutionary interest, including many life history and behavioral traits, have inherently nonnormal distribu...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.186536
更新日期：2016-11-01 00:00:00
abstract：:Condensins are evolutionarily conserved protein complexes that are required for chromosome segregation during cell division and genome organization during interphase. In Caenorhabditis elegans, a specialized condensin, which forms the core of the dosage compensation complex (DCC), binds to and represses X chromosome t...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302254
更新日期：2019-07-01 00:00:00
abstract：:In the yeast Saccharomyces cerevisiae, rim1, 8, 9, or 13 mutations cause four phenotypes: poor growth at low temperature, altered colony morphology, inefficient sporulation due to reduced expression of the meiotic activator IME1, and, as shown here, defective invasive growth. In this report, we have determined the rel...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Hybrid sterility is a common form of reproductive isolation between nascent species. Although hybrid sterility is routinely documented and genetically dissected in speciation studies, its developmental basis is rarely examined, especially in generations beyond the F1 generation. To identify phenotypic and genetic dete...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301658
更新日期：2018-12-01 00:00:00
abstract：:Research on the genetics of natural populations was revolutionized in the 1990s by methods for genotyping noninvasively collected samples. However, these methods have remained largely unchanged for the past 20 years and lag far behind the genomics era. To close this gap, here we report an optimized laboratory protocol...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.187492
更新日期：2016-06-01 00:00:00
abstract：:We previously identified Caenorhabditis elegans mutants in which certain of the six vulval precursor cells adopt fates normally expressed by other vulval precursor cells. These mutants define genes that appear to function in the response to an intercellular signal that induces vulval development. The multivulva (Muv) ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Understanding variation in allele frequencies across populations is a central goal of population genetics. Classical models for the distribution of allele frequencies, using forward simulation, coalescent theory, or the diffusion approximation, have been applied extensively for demographic inference, medical study des...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.117.200493
更新日期：2017-07-01 00:00:00
abstract：:An extending axon growth cone is subjected to attractant and repellent cues. It is not clear how these growth cones discriminate the two opposing forces and select their projection paths. Here, we report that in the Drosophila nerve cord the growth cones of longitudinal tracts are subjected to attraction by the Netrin...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.036863
更新日期：2005-05-01 00:00:00
abstract：:We previously identified a transcriptional regulatory element, which we call NRE(DIT), that is required for repression of the sporulation-specific genes, DIT1 and DIT2, during vegetative growth of Saccharomyces cerevisiae. Repression through this element is dependent on the Ssn6-Tup1 corepressor. In this study, we sho...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:Biology is characterized by complex interactions between phenotypes, such as recursive and simultaneous relationships between substrates and enzymes in biochemical systems. Structural equation models (SEMs) can be used to study such relationships in multivariate analyses, e.g., with multiple traits in a quantitative g...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.112979
更新日期：2010-06-01 00:00:00
abstract：:In Caenorhabditis elegans embryos, specification of the germ lineage depends on PIE-1, a maternal protein that blocks mRNA transcription in germline blastomeres. Studies in mammalian cell culture have suggested that PIE-1 inhibits P-TEFb, a kinase that phosphorylates serine 2 in the carboxyl-terminal domain (CTD) repe...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.083212
更新日期：2008-01-01 00:00:00
abstract：:The Arp2/3 complex is an essential component of the actin cytoskeleton in yeast and is required for the movement of actin patches. In an attempt to identify proteins that interact with this complex in the fission yeast Schizosaccharomyces pombe, we sought high-copy suppressors of the S. pombe arp3-c1 mutant, and have ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Trichome development in Arabidopsis thaliana is a well-characterized model for the study of plant cell differentiation. Two genes that play an essential role in the initiation of trichome development are GL1 and TTG. Mutations in either gene prevent the initiation of most trichomes. The GL1 gene encodes a myb-related ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:G-protein-coupled receptors (GPCRs) are integral membrane proteins that initiate stimulus-dependent activation of cognate heterotrimeric G-proteins, triggering ensuing downstream cellular responses. Tight regulation of GPCR-evoked pathways is required because prolonged stimulation can be detrimental to an organism. St...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.186122
更新日期：2016-05-01 00:00:00
abstract：:We examined the effect of a single variant repeat on the stability of a 51-base pair (bp) microsatellite (poly GT). We found that the insertion stabilizes the microsatellite about fivefold in wild-type strains. The stabilizing effect of the variant base was also observed in strains with mutations in the DNA mismatch r...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
abstract：:Repair replication of DNA has been studied in first instar larvae of Drosophila melanogaster with isopycnic centrifugation techniques. Larvae were fed BUdR, FUdR, streptomycin, penicillin, and Fungazone for two to four hours prior to exposure to UV, X-rays, MMS, or EMS. Feeding was continued for four hours in the pres...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1974-08-01 00:00:00
abstract：:Within the context of a conservation program the management of subdivided populations implies a compromise between the control of the global genetic diversity, the avoidance of high inbreeding levels, and, sometimes, the maintenance of a certain degree of differentiation between subpopulations. We present a dynamic an...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.083816
更新日期：2008-05-01 00:00:00
abstract：:In multigene families, variation among loci and alleles can contribute to trait evolution. We explored patterns of functional and genetic variation in six duplicated Arabidopsis thaliana trypsin inhibitor (ATTI) loci. We demonstrate significant variation in constitutive and herbivore-induced transcription among ATTI l...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.166.3.1419
更新日期：2004-03-01 00:00:00
abstract：:Laboratory crosses between wild strains of the two European house mouse subspecies Mus musculus domesticus (2n = 34) and M. m. musculus (2n = 40) were performed to analyze the selective processes involved in the non-introgression of centromeric regions of Robertsonian (Rb) fusions in the Danish hybrid zone. The chromo...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:In budding yeast, at least 10 proteins are required for formation of the double-strand breaks (DSBs) that initiate meiotic recombination. Spo11 is the enzyme responsible for cleaving DNA and is found in a complex that also contains Ski8, Rec102, and Rec104. The Mre11/Rad50/Xrs2 complex is required for both DSB formati...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.058768
更新日期：2006-08-01 00:00:00
abstract：:While mitochondria are renowned for their role in energy production, they also perform several other integral functions within the cell. Thus, it is not surprising that mitochondrial dysfunction can negatively impact cell viability. Although mitochondria have received an increasing amount of attention in recent years,...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.117200
更新日期：2010-08-01 00:00:00
abstract：:doublesex (dsx) is unusual among the known sex-determination genes of Drosophila melanogaster in that functional homologs are found in distantly related species. In flies, dsx occupies a position near the bottom of the sex determination hierarchy. It is expressed in male- and female-specific forms and these proteins f...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
abstract：:Four species of the Hawaiian Drosophila planitibia subgroup which are homosequential in their polytene chromosomes are resident on the islands of Molokai, Maui and Hawaii. Comparisons of DNA sequence divergence in these four have been made by hybridization of total single-copy radiolabeled tracer DNA from each of the ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1983-06-01 00:00:00
abstract：:The formation of dense core granules (DCGs) requires both the sorting of granule contents from other secretory proteins and a postsorting maturation process. The Tetrahymena thermophila strain SB281 fails to synthesize DCGs, and previous analysis suggested that the defect lay at or near the sorting step. Because this ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:137Cs-gamma irradiation of bacteriophage T4 induces large deletions plus a variety of types of point mutations. All mutations arise with single-hit kinetics, and all by a misrepair process. The estimated point mutation rate is 1.5 X 10(-9) per locus per rad. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：:Two closely related bacteriophage, varphiCr30 and varphiCr35, are the first bacteriophage shown to mediate generalized transduction in Caulobacter crescentus. Unlike most other transducing phage, they are virulent and do not form any sort of lysogenic relationship with their host. However, they are rather inefficient ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-11-01 00:00:00
abstract：:Methodologies for mapping of genes underlying quantitative traits have advanced considerably but have not been accompanied by a parallel development of new population structures. We present a novel population consisting of 50 introgression lines (ILs) originating from a cross between the green-fruited species Lycopers...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Statistical tests of the neutrality hypothesis that are based on the sampling theory of Ewens (1972) require the assumption of panmixia. It is proposed that for a population comprising numerous local populations with weak gene flow among them, tests based on Ewens' theory can be applied separately to samples from each...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
