Abstract:
:Repair replication of DNA has been studied in first instar larvae of Drosophila melanogaster with isopycnic centrifugation techniques. Larvae were fed BUdR, FUdR, streptomycin, penicillin, and Fungazone for two to four hours prior to exposure to UV, X-rays, MMS, or EMS. Feeding was continued for four hours in the presence of (3)HBUdR and DNA was isolated from whole larvae. Repair replication is stimulated by each of these agents. MMS is about 10 times as potent as EMS in stimulating repair synthesis. A dose of 200 ergs/mm(2) largely saturates the level of repair replication observed after UV irradiation. Repair replication rises between 0 and 80,000 R of X-rays before falling off. Semiconservative synthesis is seriously inhibited above a dose of 40,000 R of X-rays. Photorepair has been detected as a reduction in repair synthesis resulting from post-irradiation exposure to photoreactivating light. The same treatment has no detectable effect on X-ray-stimulated repair replication. Repair replication is insensitive to the presence of caffeine or hydroxyurea during the final incubation, although semiconservative synthesis is strongly inhibited by these agents. A mixture of BUdR and (3)HTdR can be used to replace (3)HBUdR in detecting repair replication.
journal_name
Geneticsjournal_title
Geneticsauthors
Boyd JB,Presley JMsubject
Has Abstractpub_date
1974-08-01 00:00:00pages
687-700issue
4eissn
0016-6731issn
1943-2631journal_volume
77pub_type
杂志文章相关文献
GENETICS文献大全abstract::Gain or loss of chromosomes resulting in aneuploidy can be important factors in cancer and adaptive evolution. Although chromosome gain is a frequent event in eukaryotes, there is limited information on its genetic control. Here we measured the rates of chromosome gain in wild-type yeast and sister chromatid cohesion ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.159202
更新日期:2014-02-01 00:00:00
abstract::Mutants of Paramecium caudatum with abnormal swimming behavior or responses to cations were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Some of the mutants, like pawn in P. tetraurelia, cannot swim backward and are called CNR. Seven independently obtained CNR mutants belonged to three complement...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
abstract::A major challenge in undergraduate life science curricula is the continual evaluation and development of courses that reflect the constantly shifting face of contemporary biological research. Synthetic biology offers an excellent framework within which students may participate in cutting-edge interdisciplinary researc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.096784
更新日期:2009-01-01 00:00:00
abstract::Though much has been learned about the process of ovarian follicle maturation through studies of oogenesis in both vertebrate and invertebrate systems, less is known about how follicles form initially. In Drosophila, two somatic follicle stem cells (FSCs) in each ovariole give rise to all polar cells, stalk cells, and...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.109538
更新日期:2010-02-01 00:00:00
abstract::We provide a genetic analysis of the meiotic drive system on maize abnormal chromosome 10 (Ab10) that causes preferential segregation of specific chromosomal regions to the reproductive megaspore. The data indicate that at least four chromosomal regions contribute to meiotic drive, each providing distinct functions th...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00
abstract::The RPS5 disease resistance gene of Arabidopsis mediates recognition of Pseudomonas syringae strains that possess the avirulence gene avrPphB. By screening for loss of RPS5-specified resistance, we identified five pbs (avrPphB susceptible) mutants that represent three different genes. Mutations in PBS1 completely bloc...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::We present theoretical explanations and show through simulation that the individual admixture proportion estimates obtained by using ancestry informative markers should be seen as an error-contaminated measurement of the underlying individual ancestry proportion. These estimates can be used in structured association t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.075408
更新日期:2007-07-01 00:00:00
abstract::Crossovers (COs) between homologous chromosomes are critical for meiotic chromosome segregation and form in the context of the synaptonemal complex (SC), a meiosis-specific structure that assembles between aligned homologs. During Caenorhabditis elegans meiosis, central region components of the SC (SYP proteins) are e...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302625
更新日期:2019-10-01 00:00:00
abstract::This FlyBook chapter summarizes the history and the current state of our understanding of the Wingless signaling pathway. Wingless, the fly homolog of the mammalian Wnt oncoproteins, plays a central role in pattern generation during development. Much of what we know about the pathway was learned from genetic and molec...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.117.300157
更新日期:2018-04-01 00:00:00
abstract::Using the biological species definition, yeasts of the genus Saccharomyces sensu stricto comprise six species and one natural hybrid. Previous work has shown that reproductive isolation between the species is due primarily to sequence divergence acted upon by the mismatch repair system and not due to major gene differ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.062166
更新日期:2006-10-01 00:00:00
abstract::There have been several attempts to estimate the average dominance (ratio of heterozygous to homozygous effects) of spontaneous deleterious mutations in Drosophila melanogaster, but these have given inconsistent results. We investigated whether transposable element (TE) insertions have higher average dominance for egg...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::Circadian clocks organize the metabolism, physiology, and behavior of organisms throughout the day-night cycle by controlling daily rhythms in gene expression at the transcriptional and post-transcriptional levels. While many transcription factors underlying circadian oscillations are known, the splicing factors that ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300139
更新日期:2017-10-01 00:00:00
abstract::Sequence divergence among the mitochondrial (mt) DNA of nine Lycopersicon and two closely related Solanum species was estimated using the shared fragment method. A portion of each mt genome was highlighted by probing total DNA with a series of plasmid clones containing mt-specific DNA fragments from Lycopersicon penne...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::Reversion from the suppressed to nonsuppressed phenotype in strains of geno;type SUQ5 [psi+] ade2-1 his5-2 lys1-1 can1-100 ura3-1 has been induced by treatment with ethyl methanesulphonate, nitrosoguanidine or UV (254 nm) light. Spontaneously occurring revertants have also been selected by two different methods. Rever...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
abstract::The Duchenne muscular dystrophy (Dmd) locus lies in a region of the X chromosome that experiences a high rate of recombination and is thus expected to be relatively unaffected by the effects of selection on nearby genes. To provide a picture of nucleotide variability at a high-recombination locus in humans, we sequenc...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::We investigated genetic polymorphism in the Caenorhabditis elegans srh and str chemoreceptor gene families, each of which consists of approximately 300 genes encoding seven-pass G-protein-coupled receptors. Almost one-third of the genes in each family are annotated as pseudogenes because of apparent functional defects...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.035329
更新日期:2005-04-01 00:00:00
abstract::Many genetic variants identified in genome-wide association studies (GWAS) are associated with multiple, sometimes seemingly unrelated, traits. This motivates multi-trait association analyses, which have successfully identified novel associated loci for many complex diseases. While appealing, most existing methods foc...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303242
更新日期:2020-08-01 00:00:00
abstract::Biofilms are microbial communities that form on surfaces. They are the primary form of microbial growth in nature and can have detrimental impacts on human health. Some strains of the budding yeast Saccharomyces cerevisiae form colony biofilms, and there is substantial variation in colony architecture between biofilm-...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.142067
更新日期:2013-02-01 00:00:00
abstract::At pH 6.8, a substantial fraction of recombinant colonies obtained from conjugation with an HfrH donor contained multiple recombinant classes in a single colony (polygenotype colony). In contrast, when the conjugation was performed at pH 7.6, the number of polygenotypic colonies was drastically reduced, and the recomb...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract::Diversity of the founding population of Human Immunodeficiency Virus Type 1 (HIV-1) transmissions raises many important biological, clinical, and epidemiological issues. In up to 40% of sexual infections, there is clear evidence for multiple founding variants, which can influence the efficacy of putative prevention me...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300284
更新日期:2017-11-01 00:00:00
abstract::The rate of adaptive evolution of a population ultimately depends on the rate of incorporation of beneficial mutations. Even beneficial mutations may, however, be lost from a population since mutant individuals may, by chance, fail to reproduce. In this paper, we calculate the probability of fixation of beneficial mut...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::We have studied lethal mutations in the single calmodulin gene (Cam) of Drosophila to gain insight into the in vivo functions of this important calcium sensor. As a result of maternal calmodulin (CaM) in the mature egg, lethality is delayed until the postembryonic stages. Prior to death in the first larval instar, Cam...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::It is well known that an allele causing increased recombination is expected to proliferate as a result of genetic drift in a finite population undergoing selection, without requiring other mechanisms. This is supported by recent simulations apparently demonstrating that, in small populations, drift is more important t...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::Genic variation in natural populations of Drosophila simulans was surveyed using allozymic and two-dimensional electrophoretic techniques. Consistent with some previous reports, allozymic heterozygosity appeared lower than in the sibling species D. melanogaster (0.07 vs. 0.16). No variation was detected by two-dimensi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Translocations between the two large autosomes were scored in the progeny of males that had been fed mitomycin-C. The frequencies were the same in spermatogonia sampled after 12 and 25 days and in spermatozoa stored for the same periods in untreated females. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::The Drosophila melanogaster ovo gene is required for survival and differentiation of female germline cells, apparently playing a role in germline sex determination. We recovered 60 kb of genomic DNA from its genetic location at 4E1,2 on the X chromosome. A transcription unit coding for an apparently female-specific ge...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::The type of variegation of eyes of white-mottled mutants of D. hydei, either small-spotted or large-spotted, depends on the specific chromosome rearrangement involved. This distinction between mutants, though handsome, is not absolute because very seldomly small-spotted types do show a larger pigment aggregate and som...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1973-10-01 00:00:00
abstract::Pontocerebellar hypoplasia type 1b (PCH1b) is an autosomal recessive disorder that causes cerebellar hypoplasia and spinal motor neuron degeneration, leading to mortality in early childhood. PCH1b is caused by mutations in the RNA exosome subunit gene, EXOSC3 The RNA exosome is an evolutionarily conserved complex, con...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.195917
更新日期:2017-01-01 00:00:00
abstract::Stress caused by the increased salinity of irrigated fields impairs plant growth and is one of the major constraints that limits crop productivity in many important agricultural areas. As a contribution to solving such agronomic problems, we have carried out a large-scale screening for Arabidopsis thaliana mutants ind...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Plants with mutations in one of three maize genes, mop1, rmr1, and rmr2, are defective in paramutation, an allele-specific interaction that leads to meiotically heritable chromatin changes. Experiments reported here demonstrate that these genes are required to maintain the transcriptional silencing of two different tr...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.058669
更新日期:2006-07-01 00:00:00