Abstract：
:We have studied lethal mutations in the single calmodulin gene (Cam) of Drosophila to gain insight into the in vivo functions of this important calcium sensor. As a result of maternal calmodulin (CaM) in the mature egg, lethality is delayed until the postembryonic stages. Prior to death in the first larval instar, Cam nulls show a striking behavioral abnormality (spontaneous backward movement) whereas a mutation, Cam7, that results in a single amino acid change (V91G) produces a very different phenotype: short indented pupal cases and pupal death with head eversion defects. We show here that the null behavioral phenotype originates in the nervous system and involves a CaM function that requires calcium binding to all four sites of the protein. Further, backward movement can be induced in hypomorphic mutants by exposure to high light levels. In contrast, the V91G mutation specifically affects the musculature and causes abnormal calcium release in response to depolarization of the muscles. Genetic interaction studies suggest that failed regulation of the muscle calcium release channel, the ryanodine receptor, is the major defect underlying the Cam7 phenotype.
journal_name
Geneticsjournal_title
Geneticsauthors
Wang B,Sullivan KM,Beckingham Kkeywords:
subject
Has Abstractpub_date
2003-11-01 00:00:00pages
1255-68issue
3eissn
0016-6731issn
1943-2631journal_volume
165pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The aim of many genetic studies is to locate the genomic regions (called quantitative trait loci, QTL) that contribute to variation in a quantitative trait (such as body weight). Confidence intervals for the locations of QTL are particularly important for the design of further experiments to identify the gene or genes...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.061549
更新日期：2006-09-01 00:00:00
abstract：:Genetic analyses of nine traits associated with stem water-soluble carbohydrate (SWSC) accumulation and remobilization at grain-filling period under drought stress (DS) and well-watered (WW) conditions were undertaken using doubled haploid lines (DHLs) derived from two Chinese common wheat cultivars. Some significantl...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.068361
更新日期：2007-05-01 00:00:00
abstract：:Samples of Rhynchosporium secalis were collected from two experimental barley populations known to carry a diverse array of alleles for resistance to this fungal pathogen. Classification of 163 isolates for four putative isozyme systems, a colony color dimorphism and 20 ribosomal DNA restriction fragment length varian...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:Gene expression in Anopheles gambiae shows a deficiency of testis-expressed genes on the X chromosome associated with an excessive movement of retrogene duplication. We suggest that the degeneration of sex chromosomes in this monandrous species is likely the result of pressures from X inactivation, dosage compensation...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.133157
更新日期：2011-11-01 00:00:00
abstract：:Position effect variegation results from chromosome rearrangements which translocate euchromatic genes close to the heterochromatin. The euchromatin-heterochromatin association is responsible for the inactivation of these genes in some cell clones. In Drosophila melanogaster the Y chromosome, which is entirely heteroc...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:The identification of a second structural gene mutation at the feline arylsulfatase B locus (MPS VIb) provided the opportunity to investigate the expression of allelism by characterization of the residual enzymatic activity in feline mucopolysaccharidosis VI, an animal analogue of human Maroteaux-Lamy syndrome. Mating...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:Gene targeting using sequence insertion vectors generally results in integration of one copy of the targeting vector generating a tandem duplication of the cognate chromosomal region of homology. However, occasionally the target locus is found to contain >1 copy of the integrated vector. The mechanism by which the lat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:Drosophila melanogaster heterochromatin protein 2 (HP2) interacts with heterochromatin protein 1 (HP1). In polytene chromosomes, HP2 and HP1 colocalize at the chromocenter, telomeres, and the small fourth chromosome. We show here that HP2 is present in the arms as well as the centromeric regions of mitotic chromosomes...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.057604
更新日期：2006-11-01 00:00:00
abstract：:Frequent nonhomologous recombination has been previously postulated to explain the 1045-bp insertion in one mitochondrial sperm-transmitted haplotype of Mytilus galloprovincialis. Such recombination would lead to the disruption of gene order and so the existence of a specific mechanism for maintaining the same gene or...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.065698
更新日期：2007-02-01 00:00:00
abstract：:Electron microscope serial section reconstruction analysis of all zygotene-pachytene nuclei of meiotic cells from three wild-type germaria (a subunit of the ovary containing the early meiotic stages arrayed in temporal developmental sequence) of Drosophila melanogaster females corroborates and extends earlier observat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:We previously identified a transcriptional regulatory element, which we call NRE(DIT), that is required for repression of the sporulation-specific genes, DIT1 and DIT2, during vegetative growth of Saccharomyces cerevisiae. Repression through this element is dependent on the Ssn6-Tup1 corepressor. In this study, we sho...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:It has been hypothesized that the ratio of X-linked to autosomal sequence diversity is influenced by unequal sex ratios in Drosophila melanogaster populations. We conducted a genome scan of single nucleotide polymorphism (SNP) of 378 autosomal loci in a derived European population and of a subset of 53 loci in an ance...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.074922
更新日期：2007-09-01 00:00:00
abstract：:The inbred mouse strain, C57BL/6J, was derived from mice of the Mus musculus complex. C57BL/6J can be crossed in the laboratory with a closely related mouse species, M. spretus to produce fertile offspring; however there has been no previous evidence of gene flow between M. spretus and M. musculus in nature. Analysis ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
abstract：:We develop the theory for computing the joint frequency spectra of alleles in two closely related species. We allow for arbitrary population growth in both species after they had a common ancestor. We focus on the case in which a single chromosome is sequenced from one of the species. We use classical diffusion theory...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.070730
更新日期：2007-09-01 00:00:00
abstract：:In this article we investigate multiplicative effects between genes in relation to heterosis. The extensive literature on heterosis due to multiplicative effects between characters is reviewed, as is earlier work on the genetic description of heterosis. A two-locus diallelic model of arbitrary gene action is used to d...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：:The TATA-binding protein (TBP) is composed of a highly conserved core domain sufficient for TATA-element binding and preinitiation complex formation as well as a highly divergent N-terminal region that is dispensable for yeast cell viability. In vitro, removal of the N-terminal region domain enhances TBP-TATA associat...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:Mutations in mes-2, mes-3, mes-4, and mes-6 result in maternal-effect sterility: hermaphrodite offspring of mes/mes mothers are sterile because of underproliferation and death of the germ cells, as well as an absence of gametes. Mutant germ cells do not undergo programmed cell death, but instead undergo a necrotic-typ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:To identify hypothesized missing components of the synaptic G alpha(o)-G alpha(q) signaling network, which tightly regulates neurotransmitter release, we undertook two large forward genetic screens in the model organism C. elegans and focused first on mutations that strongly rescue the paralysis of ric-8(md303) reduct...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.032334
更新日期：2005-02-01 00:00:00
abstract：:The large-scale systematic variation in nucleotide composition along mammalian and avian genomes has been a focus of the debate between neutralist and selectionist views of molecular evolution. Here we test whether the compositional variation is due to mutation bias using two new tests, which do not assume composition...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：:The concept of haplotype blocks has been shown to be useful in genetics. Fields of application range from the detection of regions under positive selection to statistical methods that make use of dimension reduction. We propose a novel approach ("HaploBlocker") for defining and inferring haplotype blocks that focuses ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302283
更新日期：2019-08-01 00:00:00
abstract：:Positive selection in genes and genomes can point to the evolutionary basis for differences among species and among races within a species. The detection of positive selection can also help identify functionally important protein regions and thus guide protein engineering. Many existing tests for positive selection ar...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.074732
更新日期：2007-08-01 00:00:00
abstract：:To study the evolutionary role played by differential male and female fertility (sexual asymmetry) both between individuals and over the life span within single individuals, the terms "intrinsic male fertility" and "intrinsic female fertility" are introduced. With the help of these terms, the concept of sexual asymmet...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：:Mutations were induced in synchronous Chinese hamster cells by bromodeoxyuridine (BUdR) incorporated into cells for one-hour periods in the cell cycle. There was a very pronounced temporal dependence during the first half of the DNA synthesis period for the induction of damage leading to 6-thioguanine (6TG) and ouabai...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
abstract：:Sir2 is a highly conserved NAD+-dependent histone deacetylase that functions in heterochromatin formation and promotes replicative life span (RLS) in the budding yeast, Saccharomyces cerevisiae Within the yeast rDNA locus, Sir2 is required for efficient cohesin recruitment and maintaining the stability of the tandem a...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.119.302047
更新日期：2019-05-01 00:00:00
abstract：:Sister-chromatid exchanges (SCE) were studied in allium cepa L. meristematic cells at the second and third divisions after BrdUrd-substitution during just the first or during the second and third cycles, respectively. The observed SCE nonreciprocal/reciprocal ratios detected at the third division in both experiments, ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-08-01 00:00:00
abstract：:The variances of genetic variances within and between finite populations were systematically studied using a general multiple allele model with mutation in terms of identity by descent measures. We partitioned the genetic variances into components corresponding to genetic variances and covariances within and between l...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:This report examines five different naturally occurring alcohol dehydrogenase-1 alleles via the recombinational behavior of Adh1(-) mutants induced within them. Twenty-two biochemically characterized Adh1(-) mutants have been assessed for ability to recombine intragenically, using data generated by specifically staini...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:Enhancer of split [E(spl)] is one of the neurogenic loci of Drosophila and, as such, is required for normal segregation of neural and epidermal cell progenitors. Genetic observations indicate that the E(spl) locus is in fact a gene complex comprising a cluster of related genes and that other genes of the region are al...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:The noncanonical Frizzled/planar cell polarity (PCP) pathway regulates establishment of polarity within the plane of an epithelium to generate diversity of cell fates, asymmetric, but highly aligned structures, or to orchestrate the directional migration of cells during convergent extension during vertebrate gastrulat...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.181438
更新日期：2016-03-01 00:00:00
abstract：:In this work we study how mutations that change physical properties of cell proteins (stability) affect population survival and growth. We present a model in which the genotype is presented as a set folding free energies of cell proteins. Mutations occur upon replication, so stabilities of some proteins in daughter ce...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.106492
更新日期：2009-10-01 00:00:00
