Abstract：
:In budding yeast, at least 10 proteins are required for formation of the double-strand breaks (DSBs) that initiate meiotic recombination. Spo11 is the enzyme responsible for cleaving DNA and is found in a complex that also contains Ski8, Rec102, and Rec104. The Mre11/Rad50/Xrs2 complex is required for both DSB formation and DSB processing. In this article we investigate the functions of the remaining three proteins--Mer2, Mei4, and Rec114--with particular emphasis on Mer2. The Mer2 protein is present in vegetative cells, but it increases in abundance and becomes phosphorylated specifically during meiotic prophase. Mer2 localizes to distinct foci on meiotic chromosomes, with foci maximally abundant prior to the formation of synaptonemal complex. If DSB formation is blocked (e.g., by a spo11 mutation), dephosphorylation of Mer2 and its dissociation from chromosomes are delayed. We have also found that the Mei4 and Rec114 proteins localize to foci on chromosomes and these foci partially colocalize with each other and with Mer2. Furthermore, the three proteins co-immunoprecipitate. Mer2 does not show significant colocalization with Mre11 or Rec102 and Mer2 does not co-immunoprecipitate with Rec102. We propose that Mer2, Mei4, and Rec114 form a distinct complex required for DSB formation.
journal_name
Geneticsjournal_title
Geneticsauthors
Li J,Hooker GW,Roeder GSdoi
10.1534/genetics.106.058768subject
Has Abstractpub_date
2006-08-01 00:00:00pages
1969-81issue
4eissn
0016-6731issn
1943-2631pii
genetics.106.058768journal_volume
173pub_type
杂志文章相关文献
GENETICS文献大全abstract：:Understanding the transmission dynamics of infectious diseases is important for both biological research and public health applications. It has been widely demonstrated that statistical modeling provides a firm basis for inferring relevant epidemiological quantities from incidence and molecular data. However, the comp...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.180034
更新日期：2016-03-01 00:00:00
abstract：:A flight test box was developed and used in the isolation and initial characterization of Drosophila melanogaster mutants defective in flight behavior. Forty-eight mutants were isolated from F(1) progeny of ethyl methanesulfonate-treated males. Genetic mapping and complementation tests show that the mutations reside a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：:All living organisms require nutrient minerals for growth and have developed mechanisms to acquire, utilize, and store nutrient minerals effectively. In the aqueous cellular environment, these elements exist as charged ions that, together with protons and hydroxide ions, facilitate biochemical reactions and establish ...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.112.147207
更新日期：2013-03-01 00:00:00
abstract：:Gastrulation is fundamental to the development of multicellular animals. Along with neurulation, gastrulation is one of the major processes of morphogenesis in which cells or whole tissues move from the surface of an embryo to its interior. Cell internalization mechanisms that have been discovered to date in Caenorhab...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.119.300240
更新日期：2020-02-01 00:00:00
abstract：:Vertebrate retrotransposons have been used extensively for phylogenetic analyses and studies of molecular evolution. Information can be obtained from specific inserts either by comparing sequence differences that have accumulated over time in orthologous copies of that insert or by determining the presence or absence ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：:Candida albicans, which is diploid, possesses a single mating-type (MTL) locus on chromosome 5, which is normally heterozygous (a/alpha). To mate, C. albicans must undergo MTL homozygosis to a/a or alpha/alpha. Three possible mechanisms may be used in this process, mitotic recombination, gene conversion, or loss of on...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.033167
更新日期：2005-03-01 00:00:00
abstract：:The mutation rates of specific loci and chromosome regions were estimated for two types of dysgenic hybrid males. These came from crosses between P or Q males and M females in the P-M system of hybrid dysgenesis. The M X P hybrids were the more mutable for each of the loci and chromosome regions tested. The Beadex loc...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:Robust and synchronous repression of E2F-dependent gene expression is critical to the proper timing of cell cycle exit when cells transition to a postmitotic state. Previously NuA4 was suggested to act as a barrier to proliferation in Drosophila by repressing E2F-dependent gene expression. Here we show that NuA4 activ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.188581
更新日期：2016-07-01 00:00:00
abstract：:The HML and HMR loci carry unexpressed copies of MATa and MAT alpha information, and a replica of that information is transposed to MAT during mating-type interchange in Saccharomyces yeasts. A negative control mechanism keeps silent the information located at the HML and HMR loci. We mapped these loci by constructing...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:Telomeric DNA repeats are key features of chromosomes that allow the maintenance of integrity and stability in the telomeres. However, interstitial telomere sites (ITSs) can also be found along the chromosomes, especially near the centromere, where they may appear following chromosomal rearrangements like Robertsonian...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.114.166421
更新日期：2014-08-01 00:00:00
abstract：:A causal link between deficiency of the cytochrome respiratory pathway and life span was previously shown in the filamentous fungus Podospora anserina. To gain more insight into the relationship between mitochondrial function and life span, we have constructed a strain carrying a thermosensitive mutation of the gene o...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.033837
更新日期：2005-03-01 00:00:00
abstract：:The ability of a virus population to colonize a novel host is predicted to depend on the equilibrium frequency of potential colonists (i.e., genotypes capable of infecting the novel host) in the source population. In this study, we investigated the determinants of the equilibrium frequency of potential colonists in th...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.064634
更新日期：2007-06-01 00:00:00
abstract：:A simple method of replica plating has been used to isolate mutants of Neurospora crassa that have increased sensitivity to methyl methanesulfonate (MMS) and/or to histidine. Twelve mutants with increased sensitivity to MMS and one mutant with increased sensitivity to histidine showed Mendelian segregation of the muta...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:The larval cuticle protein (Lcp) cluster in Drosophila melanogaster contains four functional genes and a closely related pseudogene. A 630-bp fragment including the larval cuticle pseudogene locus (Lcp psi) was nucleotide sequenced in 10 strains of D. melanogaster and a 458-bp Lcp psi fragment from D. simulans was als...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:In eukaryotic cells, the organization of genomic DNA into chromatin regulates many biological processes, from the control of gene expression to the regulation of chromosome segregation. The proper maintenance of this structure upon cell division is therefore of prime importance during development for the maintenance o...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.190785
更新日期：2017-01-01 00:00:00
abstract：:A procedure permitting deletion scanning of potential cis-regulatory sequences within a transgene whose genomic position remains fixed was applied to the study of the upstream sequences of the ecdysteroid-inducible Fat-body-protein-1 (Fbp1) gene. Deficiencies were induced in a Fbp1:Adh fusion transgene by means of a s...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:We show that major chromosomal rearrangements can occur upon T-DNA transformation of Arabidopsis thaliana. In the ACL4 line, two T-DNA insertion loci were found; one is a tandem T-DNA insert in a head-to-head orientation, and the other is a truncated insert with only the left part of the T-region. The four flanking DN...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:The F(1) hybrids of Nicotiana suaveolens (subgenus Petunioides, 2n = 32) and N. glutinosa (subgenus Tabacum, 2n = 24), were examined during their development, from seedlings to mature plants. It was observed that in the hybrids, there was a progressive change of dominant N. glutinosa morphological characteristics towa...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-04-01 00:00:00
abstract：:The fission yeast Schizosaccharomyces pombe does not form tripartite synaptonemal complexes during meiotic prophase, but axial core-like structures (linear elements). To probe the relationship between meiotic recombination and the structure, pairing, and segregation of meiotic chromosomes, we genetically and cytologic...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:The effective population size (N(e)) is notoriously difficult to accurately estimate in wild populations as it is influenced by a number of parameters that are difficult to delineate in natural systems. The different methods that are used to estimate N(e) are affected variously by different processes at the population...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.111.136580
更新日期：2012-06-01 00:00:00
abstract：:Genome-wide association mapping is a popular method for using natural variation within a species to generate a genotype-phenotype map. Statistical association between an allele at a locus and the trait in question is used as evidence that variation at the locus is responsible for variation of the trait. Indirect assoc...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.121665
更新日期：2010-11-01 00:00:00
abstract：:Paramutation generates heritable changes affecting regulation of specific alleles found at several Zea mays (maize) loci that encode transcriptional regulators of anthocyanin biosynthetic genes. Although the direction and extent of paramutation is influenced by poorly understood allelic interactions occurring in diplo...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.045260
更新日期：2005-10-01 00:00:00
abstract：:The supramolecular striatin-interacting phosphatases and kinases (STRIPAK) complex is conserved from yeast to human, and regulates a variety of key biological processes. In animals, this complex consists of the scaffold protein striatin, the protein phosphatase 2A, and kinases, such as germinal center kinase (GCK) III...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301261
更新日期：2018-09-01 00:00:00
abstract：:Crossovers (COs) generated through meiotic recombination are important for the correct segregation of homologous chromosomes during meiosis. Several models describing the molecular mechanism of meiotic recombination have been proposed. These models differ in the arrangement of heteroduplex DNA (hDNA) in recombination ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.070367
更新日期：2007-05-01 00:00:00
abstract：:The time of the pre-meiotic S-period was determined by (32)P incorporation in synchronously germinating zygospores of Chlamydomonas reinhardi at six and one-half to seven hours after the beginning of germination. Phenethyl alcohol treatment caused death of zygospores at a period one hour before the S-period, and also ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-01-01 00:00:00
abstract：:In S. commune the frequency of recombination between the two subunits, alpha and beta, of the B incompatibility factor is genetically controlled. Analysis of the progeny obtained from crosses between high- and low-recombining strains indicates that the gene controlling recombination frequency in the B factor is linked...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1973-05-01 00:00:00
abstract：:Previously we have shown that the nucleolus organizer region (NOR) of Neurospora crassa changes size frequently during the premeiotic portion of the sexual phase. Here, we have investigated whether these changes in size originate only in specific regions of the NOR, or are distributed throughout the NOR. In two specia...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:We demonstrate the involvement of suppressor of meiotic silencing-2 (sms-2(+)), a Neurospora gene coding for an Argonaute-like protein, in meiotic silencing and normal sexual development. ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:The worldwide pattern of single nucleotide polymorphism (SNP) variation is of great interest to human geneticists, population geneticists, and evolutionists, but remains incompletely understood. We studied the pattern in noncoding regions, because they are less affected by natural selection than are coding regions. Th...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：:The human pathogen Candida albicans is considered an obligate commensal of animals, yet it is occasionally isolated from trees, shrubs, and grass. We generated genome sequence data for three strains of C. albicans that we isolated from oak trees in an ancient wood pasture, and compared these to the genomes of over 200...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.118.301482
更新日期：2019-01-01 00:00:00
