Abstract:
:Centromeres control chromosome inheritance in eukaryotes, yet their DNA structure and primary sequence are hypervariable. Most animals and plants have megabases of tandem repeats at their centromeres, unlike yeast with unique centromere sequences. Centromere function requires the centromere-specific histone CENH3 (CENP-A in human), which replaces histone H3 in centromeric nucleosomes. CENH3 evolves rapidly, particularly in its N-terminal tail domain. A portion of the CENH3 histone-fold domain, the CENP-A targeting domain (CATD), has been previously shown to confer kinetochore localization and centromere function when swapped into human H3. Furthermore, CENP-A in human cells can be functionally replaced by CENH3 from distantly related organisms including Saccharomyces cerevisiae. We have used cenh3-1 (a null mutant in Arabidopsis thaliana) to replace endogenous CENH3 with GFP-tagged variants. A H3.3 tail domain-CENH3 histone-fold domain chimera rescued viability of cenh3-1, but CENH3's lacking a tail domain were nonfunctional. In contrast to human results, H3 containing the A. thaliana CATD cannot complement cenh3-1. GFP-CENH3 from the sister species A. arenosa functionally replaces A. thaliana CENH3. GFP-CENH3 from the close relative Brassica rapa was targeted to centromeres, but did not complement cenh3-1, indicating that kinetochore localization and centromere function can be uncoupled. We conclude that CENH3 function in A. thaliana, an organism with large tandem repeat centromeres, has stringent requirements for functional complementation in mitosis.
journal_name
Geneticsjournal_title
Geneticsauthors
Ravi M,Kwong PN,Menorca RM,Valencia JT,Ramahi JS,Stewart JL,Tran RK,Sundaresan V,Comai L,Chan SWdoi
10.1534/genetics.110.120337subject
Has Abstractpub_date
2010-10-01 00:00:00pages
461-71issue
2eissn
0016-6731issn
1943-2631pii
genetics.110.120337journal_volume
186pub_type
杂志文章相关文献
GENETICS文献大全abstract::Polyhomeotic is a member of the Polycomb group (Pc-G) of homeotic repressors. The proteins encoded by the Pc-G genes form repressive complexes on the polycomb group response element sites. The phP1 mutation was induced by insertion of a 1.2-kb P element into the 5' transcribed nontranslated region of the proximal poly...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-10-01 00:00:00
abstract::Vertebrate retrotransposons have been used extensively for phylogenetic analyses and studies of molecular evolution. Information can be obtained from specific inserts either by comparing sequence differences that have accumulated over time in orthologous copies of that insert or by determining the presence or absence ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-06-01 00:00:00
abstract::The pattern of mutational covariance among traits plays a central, but largely untested, role in many theories in evolutionary genetics. Here we estimate the pattern of phenotypic, environmental, and mutational correlations for a set of life-history, behavioral, and morphological traits using 67 self-fertilizing lines...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.040022
更新日期:2005-06-01 00:00:00
abstract::As a genetic mutation is passed down across generations, it distinguishes those genomes that have inherited it from those that have not, providing a glimpse of the genealogical tree relating the genomes to each other at that site. Statistical summaries of genetic variation therefore also describe the underlying geneal...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303253
更新日期:2020-07-01 00:00:00
abstract::MiRNAs bear an increasing number of functions throughout development and in the aging adult. Here we address their role in establishing sexually dimorphic traits and sexual identity in male and female Drosophila. Our survey of miRNA populations in each sex identifies sets of miRNAs differentially expressed in male and...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.169268
更新日期:2014-10-01 00:00:00
abstract::Type II topoisomerases are essential ATP-dependent homodimeric enzymes required for transcription, replication, and chromosome segregation. These proteins alter DNA topology by generating transient enzyme-linked double-strand breaks for passage of one DNA strand through another. The central role of type II topoisomera...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.144006
更新日期:2012-11-01 00:00:00
abstract::Knowledge of the rate and fitness effects of mutations is essential for understanding the process of evolution. Mutations are inherently difficult to study because they are rare and are frequently eliminated by natural selection. In the ciliate Tetrahymena thermophila, mutations can accumulate in the germline genome w...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.153536
更新日期:2013-10-01 00:00:00
abstract::The Triplo-lethal locus (Tpl) is unique in its dosage sensitivity; no other locus in Drosophila has been identified that is lethal when present in three doses. Tpl is also haplo-lethal, and its function is still a mystery. Previous workers have found it nearly impossible to mutationally inactive Tpl other than by comp...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::Much recent work in the theoretical study of adaptation has focused on the so-called strong selection-weak mutation (SSWM) limit, wherein adaptation is due to new mutations of definite selective advantage. This work, in turn, has focused on the first step (substitution) during adaptive evolution. Here we extend this t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.106757
更新日期:2009-11-01 00:00:00
abstract::Forty-eight new mutations at the am locus of Neurospora crassa have been characterized. Nineteen mutations were induced by UV; of these, eight were missense, two were frameshifts, two were nonsense, three were deletions and four were unidentified. Twenty-nine mutations were induced with nitrous acid; of these, twenty-...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::We have mapped quantitative trait loci (QTL) for Drosophila mechanosensory bristle number in six recombinant isogenic line (RIL) mapping populations, each of which was derived from an isogenic chromosome extracted from a line selected for high or low, sternopleural or abdominal bristle number and an isogenic wild-type...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::We have used the results of an experiment mapping quantitative trait loci (QTL) affecting milk yield and composition to estimate the total number of QTL affecting these traits. We did this by estimating the number of segregating QTL within a half-sib daughter design using logic similar to that used to estimate the "fa...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.077784
更新日期:2007-10-01 00:00:00
abstract::Two variations of the method of localized mutagenesis were used to introduce mutations into the 72 min region of the Escherichia coli chromosome. Twenty temperature-sensitive mutants, with linkage to markers in this region, have been examined. Each strain showed an inhibition of growth in liquid medium at 44 degrees, ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract::Nontandem terminal chromosome duplications derived from N. crassa translocation T(I-->VI)NM103 give rise mitotically to some daughter nuclei which have become euploid by loss of one or the other of the two duplicated segments. Loss of the segment in normal sequence occurs as often as loss of the translocated segment. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1977-03-01 00:00:00
abstract::Genotype by environment interactions (GEI) play a major part in shaping the genetic architecture of quantitative traits and are confounding factors in genetic studies, for example, in attempts to associate genetic variation with disease susceptibility. It is generally not known what proportion of phenotypic variation ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.086769
更新日期:2008-06-01 00:00:00
abstract::The maize ligule is an adaxial membranous structure on the leaf that develops at the boundary of the sheath and blade. The ligule and the associated auricle are dispensable structures, amenable to genetic manipulation. We present here a genetic analysis of liguleless1 (lg1) and liguleless2 (lg2), the two genes known t...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::Isogenic lines, in which chromosomes sampled from natural populations of C. melanogaster are substituted into a common genetic background, were used to detect and partially characterize autosomal factors that affect the activities of the two pentose phosphate pathway enzymes, glucose 6-phosphate dehydrogenase (G6PD) a...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract::Specialized insect mouthparts, such as those of Drosophila, are derived from an ancestral mandibulate state, but little is known about the developmental genetics of mandibulate mouthparts. Here, we study the metamorphic patterning of mandibulate mouthparts of the beetle Tribolium castaneum, using RNA interference to d...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.134296
更新日期:2012-02-01 00:00:00
abstract::Cyclin E together with its kinase partner Cdk2 is a critical regulator of entry into S phase. To identify novel genes that regulate the G1- to S-phase transition within a whole animal we made use of a hypomorphic cyclin E mutation, DmcycEJP, which results in a rough eye phenotype. We screened the X and third chromosom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.026617
更新日期:2004-09-01 00:00:00
abstract::We studied the control of pharyngeal excitation in Caenorhabditis elegans. By laser ablating subsets of the pharyngeal nervous system, we found that the MC neuron type is necessary and probably sufficient for rapid pharyngeal pumping. Electropharyngeograms showed that MC transmits excitatory postsynaptic potentials, s...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::Homolog recombination and unequal sister chromatid recombination were monitored in rad1-1/rad1-1 diploid yeast cells deficient for excision repair, and in control cells, RAD1/rad1-1, after exposure to UV irradiation. In a rad1-1/rad1-1 diploid, UV irradiation stimulated much more sister chromatid recombination relativ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Within the context of a conservation program the management of subdivided populations implies a compromise between the control of the global genetic diversity, the avoidance of high inbreeding levels, and, sometimes, the maintenance of a certain degree of differentiation between subpopulations. We present a dynamic an...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.083816
更新日期:2008-05-01 00:00:00
abstract::The genetic effects of one generation of spermatogonial X-irradiation in rats, by a single dose of 600r in one experiment and by a fractionated dose of 450r in another, were measured in three generations of their descendants. Estimates of dominant lethal mutation rates--(2 to 3) X 10-4/gamete/r--from litter size diffe...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1977-02-01 00:00:00
abstract::Genetic control over chromosome stability in the interspecific hybrid embryos of Hordeum vulgare and H. bulbosum has been hypothesized to reside on specific chromosomes. In this study, crosses between the primary trisomic lines for the seven different H. vulgare chromosomes and tetraploid H. bulbosum revealed that bot...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-10-01 00:00:00
abstract::Mutants arising spontaneously from sporulated cultures of certain strains of yeast, Saccharomyces cerevisiae, contained deletions of the CYC1 gene which controls the primary structure of iso-1-cytochrome c. At least 60 different kinds of deletions were uncovered among the 104 deletions examined and these ranged in len...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-09-01 00:00:00
abstract::Borde and colleagues reported that a reporter plasmid inserted at different genomic locations in Saccharomyces cerevisiae had different levels of meiotic recombination activity. We show that the level of recombination activity is very significantly correlated with the GC content of DNA sequences flanking the insertion...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::Ras1 GTPase is the Schizosaccharomyces pombe homolog of the mammalian Ha-Ras proto-oncoprotein. Ras1 interacts with Scd1 (aka Ral1), a presumptive guanine nucleotide exchange factor for Cdc42sp, to control organization of the cytoskeleton. In this study, we demonstrated that the scd1 deletion (scd1Delta) induced hyper...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::The DNMT1 cytosine methyltransferase enzyme contains a large ∼300-aa intrinsically disordered domain (IDD) that we previously showed regulated DNA methylation patterns in mouse ES cells. Here we generated seven mouse lines with different mutations in the IDD. Homozygous mutant mice of five lines developed normally, wi...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.173609
更新日期:2015-02-01 00:00:00
abstract::I reexamine the use of isolation by distance models as a basis for the estimation of demographic parameters from measures of population subdivision. To that aim, I first provide results for values of F-statistics in one-dimensional models and coalescence times in two-dimensional models, and make more precise earlier r...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::Translation of the chloroplast psbC mRNA in the unicellular eukaryotic alga Chlamydomonas reinhardtii is controlled by interactions between its 547-base 5' untranslated region and the products of the nuclear loci TBC1, TBC2, and possibly TBC3. In this study, a series of site-directed mutations in this region was gener...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00