Abstract:
:The Triplo-lethal locus (Tpl) is unique in its dosage sensitivity; no other locus in Drosophila has been identified that is lethal when present in three doses. Tpl is also haplo-lethal, and its function is still a mystery. Previous workers have found it nearly impossible to mutationally inactive Tpl other than by completely deleting the chromosomal region in which Tpl resides (83DE). We have utilized P-M hybrid dysgenesis in an effort to obtain new mutations of Tpl. We recovered 19 new duplications of Tpl, 15 hypomorphic mutations of Tpl (a previously rare class of mutation), and no null mutations. Surprisingly, 14 of the 15 hypomorphic alleles have no detectable P element sequences at the locus. The difficulty in recovering null mutations in Tpl suggests that it may be a complex locus, perhaps consisting of several genes with redundant functions. The relative ease with which we recovered hypomorphic alleles is in sharp contrast to previous attempts by others to mutagenize Tpl. A higher mutation rate with hybrid dysgenesis than with radiation or chemicals also suggests a peculiar genetic organization for the locus.
journal_name
Geneticsjournal_title
Geneticsauthors
Dorer DR,Christensen ACsubject
Has Abstractpub_date
1990-08-01 00:00:00pages
795-801issue
4eissn
0016-6731issn
1943-2631journal_volume
125pub_type
杂志文章相关文献
GENETICS文献大全abstract::We show how to incorporate fluctuations of the recombination rate along the chromosome into standard gene-genealogical models for the decorrelation of gene histories. This enables us to determine how small-scale fluctuations (Poissonian hot-spot model) and large-scale variations (Kong et al. 2002) of the recombination...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.103.018002
更新日期:2005-02-01 00:00:00
abstract::Mitotic cell divisions increase cell number while faithfully distributing the replicated genome at each division. The Caenorhabditis elegans embryo is a powerful model for eukaryotic cell division. Nearly all of the genes that regulate cell division in C. elegans are conserved across metazoan species, including humans...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.118.301367
更新日期:2019-01-01 00:00:00
abstract::To study the functions of DNA topoisomerase I and Hpr1 protein, a suppressor mutant of the temperature-sensitive growth of an hpr1 top1-5ts double mutant was isolated. The isolated triple mutant showed cold-sensitive growth. By complementation of this phenotype, the suppressor gene was cloned. DNA sequencing showed it...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::The past century has seen substantial theoretical and empirical progress on the genetic basis of adaptation. Over this same period, a pressing need to prevent the evolution of drug resistance has uncovered much about the potential genetic basis of persistence in declining populations. However, we have little theory to...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302890
更新日期:2020-02-01 00:00:00
abstract::Four maternal-effect sterile genes, mes-2, mes-3, mes-4, and mes-6, are essential for germline development in Caenorhabditis elegans. Homozygous mes progeny from heterozygous mothers are themselves fertile but produce sterile progeny with underproliferated and degenerated germlines. All four mes genes encode chromatin...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
abstract::In multicellular organisms, genetic programs guide cells to adopt cell fates as tissues are formed during development, maintained in adults, and repaired after injury. Here we explore how a small molecule in the environment can switch a genetic program from one fate to another. Wild-type Caenorhabditis elegans XX adul...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.169409
更新日期:2014-10-01 00:00:00
abstract::The term "micro-evo-devo" refers to the combined study of the genetic and developmental bases of natural variation in populations and the evolutionary forces that have shaped this variation. It thus represents a synthesis of the fields of evolutionary developmental biology and population genetics. As has been pointed ...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.113.156463
更新日期:2013-11-01 00:00:00
abstract::The fission yeast DNA repair gene rad21+ is essential for cell growth. To investigate the function essential for cell proliferation, we have isolated a temperature-sensitive mutant of the rad21+ gene. The mutant, rad21-K1, showed abnormal mitosis at the nonpermissive temperature. Some cells contained abnormal nuclear ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Cse4p is an essential histone H3 variant in Saccharomyces cerevisiae that defines centromere identity and is required for proper segregation of chromosomes. In this study, we investigated phenotypic consequences of Cse4p mislocalization and increased dosage of histone H3 and Cse4p, and established a direct link betwee...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.088518
更新日期:2008-05-01 00:00:00
abstract::Currently used tests of Hardy-Weinberg proportions do not take into account the nature of the alternative hypothesis, which is generally a heterozygote deficiency. Different exact tests, appropriate for small sample size and large number of alleles, are proposed in this perspective, and their properties are evaluated ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Adherence, an important virulence factor, is mediated by the EPA (Epithelial Adhesin) genes in the opportunistic pathogen Candida glabrata Expression of adhesin-encoding genes requires tight regulation to respond to harsh environmental conditions within the host. The majority of EPA genes are localized in subtelomeric...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301202
更新日期:2018-09-01 00:00:00
abstract::Bonus, a Drosophila TIF1 homolog, is a nuclear receptor cofactor required for viability, molting, and numerous morphological events. Here we establish a role for Bonus in the modulation of chromatin structure. We show that weak loss-of-function alleles of bonus have a more deleterious effect on males than on females. ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.037085
更新日期:2005-02-01 00:00:00
abstract::The Thomas Hunt Morgan Medal is awarded to an individual Genetics Society of America member for lifetime achievement in the field of genetics. It recognizes the full body of work of an exceptional geneticist. The 2016 recipient is Nancy Kleckner, who has made many significant contributions to our understanding of chro...
journal_title:Genetics
pub_type: 历史文章,杂志文章
doi:10.1534/genetics.116.197608
更新日期:2016-12-01 00:00:00
abstract::Previous studies on gene expression in aneuploids revealed numerous trans-acting dosage effects. Segmental aneuploidy of each varied chromosomal region exhibited predominantly inverse effects on several target genes. Here, dosage regulation was examined in a maize (Zea mays L.) ploidy series where the complete genomic...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-04-01 00:00:00
abstract::One of the most important parameters in population genetics is theta = 4Ne mu where Ne is the effective population size and mu is the rate of mutation per gene per generation. We study two related problems, using the maximum likelihood method and the theory of coalescence. One problem is the potential improvement of a...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::We reported previously that the product of the DNA damage-inducible gene of Saccharomyces cerevisiae, DIN7, belongs to a family of proteins that are involved in DNA repair and replication. The family includes S. cerevisiae proteins Rad2p and its human homolog XPGC, Rad27p and its mammalian homolog FEN-1, and Exonuclea...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Many complex human traits exhibit differences between sexes. While numerous factors likely contribute to this phenomenon, growing evidence from genome-wide studies suggest a partial explanation: that males and females from the same population possess differing genetic architectures. Despite this, mapping gene-by-sex (...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303120
更新日期:2020-05-01 00:00:00
abstract::Directed open reading frame (ORF) disruption and a serial selection technique in Escherichia coli and the extremely thermophilic archaeon Sulfolobus solfataricus allowed the identification of otherwise cryptic crucial and noncrucial viral open reading frames in the genome of the archaeal virus SSV1. It showed that the...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::We analyze two models of the maintenance of quantitative genetic variance in a mixed-mating system of self-fertilization and outcrossing. In both models purely additive genetic variance is maintained by mutation and recombination under stabilizing selection on the phenotype of one or more quantitative characters. The ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.176693
更新日期:2015-07-01 00:00:00
abstract::Three sex-transforming genes (ix, tra-D, and dsx) in D. melanogaster were examined with respect to possible interactions with the NSR strain SR-spirochetes. The SR-spirochetes exerted a lethal effect on XY but not on XX individuals, regardless of whether they were phenotypically males, intersexes, or females. These re...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::The Drosophila melanogaster Gld gene has multiple and diverse developmental and physiological functions. We report herein that interactions among proximal promoter elements and a cluster of intronically located enhancers and silencers specify the complex regulation of Gld that underlies its diverse functions. Gld expr...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::Although neurons are highly polarized, how neuronal polarity is generated remains poorly understood. An evolutionarily conserved inositol-producing enzyme myo-inositol monophosphatase (IMPase) is essential for polarized localization of synaptic molecules in Caenorhabditis elegans and can be inhibited by lithium, a dru...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.137844
更新日期:2012-06-01 00:00:00
abstract::We have analyzed donor and target sites of the mobile element Activator (Ac) that are altered as a result of somatic transposition from the P locus in maize. Previous genetic analysis has indicated that the two mitotic daughter lineages which result from Ac transposition from P differ in their Ac constitution at the P...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-09-01 00:00:00
abstract::In Saccharomyces cerevisiae, the Rad52 protein plays a role in both RAD51-dependent and RAD51-independent recombination pathways. We characterized a rad52 mutant, rad52-329, which lacks the C-terminal Rad51-interacting domain, and studied its role in RAD51-independent recombination. The rad52-329 mutant is completely ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::The small subunit ribosomal RNA gene (srDNA) has been used extensively for phylogenetic analyses. One common assumption in these analyses is that substitution rates are biased toward transitions. We have developed a simple method for estimating relative rates of base change that does not assume rate constancy and take...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
abstract::Hybrid male sterility (HMS) contributes to reproductive isolation commonly observed among house mouse (Mus musculus) subspecies, both in the wild and in laboratory crosses. Incompatibilities involving specific Prdm9 alleles and certain Chromosome (Chr) X genotypes are known determinants of fertility and HMS, and previ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303474
更新日期:2020-10-01 00:00:00
abstract::Nonrandom associations between alleles at different loci can be tested for using Fisher's exact test. Extensive simulations show that there is a substantial probability of obtaining significant nonrandom associations between closely or completely linked polymorphic neutral loci in a population of constant size at equi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Genic variation in natural populations of Drosophila simulans was surveyed using allozymic and two-dimensional electrophoretic techniques. Consistent with some previous reports, allozymic heterozygosity appeared lower than in the sibling species D. melanogaster (0.07 vs. 0.16). No variation was detected by two-dimensi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Dissecting the genes involved in complex traits can be confounded by multiple factors, including extensive epistatic interactions among genes, the involvement of epigenetic regulators, and the variable expressivity of traits. Although quantitative trait locus (QTL) analysis has been a powerful tool for localizing the ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.090175
更新日期:2008-12-01 00:00:00
abstract::Malaria control projects based on the introduction and spread of transgenes into mosquito populations depend on the extent of isolation between those populations. On the basis of the distribution of paracentric inversions, Anopheles gambiae has been subdivided into five subspecific chromosomal forms. Estimating gene f...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.026534
更新日期:2005-01-01 00:00:00