Abstract：
:Recent in vitro studies have shown that the periventricular subependymal zone (SEZ) of the rodent brain is capable of de novo generation of neurons and glia. There is less information available on neurogenesis in the adult human brain, and no study has shown the clonal generation of neurons and glia from in vitro-generated "neurospheres." Here we describe the isolation of proliferative stem/progenitor cells within neurospheres from two different regions, the SEZ and the hippocampus, from surgical biopsy specimens of adult (24-57 years) human brain. Using light and electron microscopy; immunocytochemistry for a variety of neuronal, glial, and developmental (including extracellular matrix; ECM) markers; and the reverse transcriptase polymerase chain reaction to demonstrate different gene transcripts found in neurospheres, it is shown that the adult human brain harbors a complex population of stem/progenitor cells that can generate neuronal and glial progeny under particular in vitro growth conditions. These methods also show that these neurospheres contain both neurons and glia and demonstrate regional similarities at the mRNA level, indicating common stem/progenitor cell types within two different neurogenic regions of the adult human brain. In addition to the synthesis of developmentally regulated molecules such as the ECM protein tenascin-C, a variety of other genes (e.g., Pax 6) and proteins (e.g. , Bcl-2) involved in cell survival and differentiation are expressed by adult human brain neurospheres.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Kukekov VG,Laywell ED,Suslov O,Davies K,Scheffler B,Thomas LB,O'Brien TF,Kusakabe M,Steindler DAdoi
10.1006/exnr.1999.7028keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
333-44issue
2eissn
0014-4886issn
1090-2430pii
S0014-4886(99)97028-0journal_volume
156pub_type
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journal_title：Experimental neurology
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journal_title：Experimental neurology
pub_type： 杂志文章,评审
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journal_title：Experimental neurology
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journal_title：Experimental neurology
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journal_title：Experimental neurology
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journal_title：Experimental neurology
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journal_title：Experimental neurology
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journal_title：Experimental neurology
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更新日期：1996-04-01 00:00:00
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journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2005.07.009
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journal_title：Experimental neurology
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journal_title：Experimental neurology
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