Adenosine and propentofylline inhibit the proliferation of cultured microglial cells.

Abstract:

:Propentofylline is a xanthine derivative that has been known to protec t neurons against ischemia-induced damage. To assess its neuroprotective mechanisms, we examined the effect of propentofylline on microglial proliferation that is thought to play an important role in neuronal damage. We determined the proliferation of microglia cultured from neonatal rat brains by measuring [3H]thymidine update. Propentofylline inhibited microglial proliferation in a dose dependent manner; EC50 was about 3 mu M. Similar results were observed with 2-chloroadenosine (agonist for A1 and A2 adenosine receptors) and 2-chloro-N6-cyclopentyladenosine (A1 receptor agonist) but not with 2-p-(2-carboxyethyl)phenethylamino-5'-N-ethyl-carboxamidoadenosine hydrochloride (A2 receptor agonist). However, 8-cyclopentyl-1,3-dipropylxathine (A1 receptor antagonist) could not reverse the inhibitory effect of propentofylline. Our results suggest that the neuroprotection by propentofylline is, as least in part, due to the direct effect of the drug on microglia and that the drug inhibits the proliferation via a certain mechanism not directly mediated by adenosine receptors.

journal_name

Exp Neurol

journal_title

Experimental neurology

authors

Si QS,Nakamura Y,Schubert P,Rudolphi K,Kataoka K

doi

10.1006/exnr.1996.0035

subject

Has Abstract

pub_date

1996-02-01 00:00:00

pages

345-9

issue

2

eissn

0014-4886

issn

1090-2430

pii

S0014-4886(96)90035-7

journal_volume

137

pub_type

杂志文章
  • A bilateral cervical contusion injury model in mice: assessment of gripping strength as a measure of forelimb motor function.

    abstract::Here, we describe a bilateral cervical contusion model for mice. Adult female mice received graded bilateral contusion injuries at cervical level 5 (C5) using a commercially available impactor (the IH device). Three separate experiments were carried out to define conditions that produce impairments in forelimb functio...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2009.09.028

    authors: Aguilar RM,Steward O

    更新日期:2010-01-01 00:00:00

  • NGF released from a polymer matrix prevents loss of ChAT expression in basal forebrain neurons following a fimbria-fornix lesion.

    abstract::Following a unilateral fimbria-fornix lesion, the delivery of nerve growth factor (NGF) to the ipsilateral lateral ventricle of the rat can prevent the lesion-induced loss of choline acetyltransferase (ChAT) expression in the ipsilateral medial septum and vertical diagonal band region. In the present study, the abilit...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/0014-4886(90)90049-x

    authors: Hoffman D,Wahlberg L,Aebischer P

    更新日期:1990-10-01 00:00:00

  • Apolipoprotein E epsilon4 allele has no effect on age at onset or duration of disease in cases of frontotemporal dementia with pick- or microvacuolar-type histology.

    abstract::Frontotemporal dementia (FTD) is the second most common cause of presenile dementia. Here we have investigated the frequency of the epsilon4 allele of the Apolipoprotein (APOE) gene in FTD and in other non-Alzheimer forms of dementia related to FTD such as Motor Neurone disease dementia, semantic dementia, progressive...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.2000.7387

    authors: Pickering-Brown SM,Owen F,Isaacs A,Snowden J,Varma A,Neary D,Furlong R,Daniel SE,Cairns NJ,Mann DM

    更新日期:2000-06-01 00:00:00

  • Effects of pathway and neuronal aging on the specificity of motor axon regeneration.

    abstract::Youth is a strong predictor of functional recovery after peripheral nerve repair, while adulthood is commonly associated with poor outcome. Identification of the factors responsible for this difference could form the basis for strategies to improve regeneration in adults. Preferential reinnervation of motor pathways b...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.2000.7538

    authors: Le TB,Aszmann O,Chen YG,Royall RM,Brushart TM

    更新日期:2001-01-01 00:00:00

  • Relation of whole muscle contractile properties to source of innervation.

    abstract::This work investigated the extent to which constituent proportions of fast- and slow-twitch muscle units determine whole muscle contractile properties. We employed several self- and cross-reinnervation models using synergistic homogeneous and heterogeneous muscles. By comparing the present data with previously publish...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/0014-4886(88)90048-9

    authors: Foehring RC,Sypert GW,Munson JB

    更新日期:1988-09-01 00:00:00

  • Aripiprazole and environmental enrichment independently improve functional outcome after cortical impact injury in adult male rats, but their combination does not yield additional benefits.

    abstract::Typical antipsychotic drugs (APDs) with D2antagonistic properties impede functional outcome after experimental traumatic brain injury (TBI) and reduce the effectiveness of environmental enrichment (EE). Here we test the hypothesis that aripiprazole (ARIP), an atypical APD with partial D2and 5-HT1Areceptor agonist acti...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2019.01.010

    authors: Besagar S,Radabaugh HL,Bleimeister IH,Meyer EA,Niesman PJ,Cheng JP,Bondi CO,Kline AE

    更新日期:2019-04-01 00:00:00

  • Muscle-derived but not centrally derived transgene GDNF is neuroprotective in G93A-SOD1 mouse model of ALS.

    abstract::Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for motoneurons (MNs), and is considered a potential agent for the treatment of amyotrophic lateral sclerosis (ALS) and other MN diseases. The effectiveness of GDNF may depend significantly upon its route of delivery to MNs. In this study w...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2006.08.028

    authors: Li W,Brakefield D,Pan Y,Hunter D,Myckatyn TM,Parsadanian A

    更新日期:2007-02-01 00:00:00

  • Degradation of chondroitin sulfate proteoglycan enhances the neurite-promoting potential of spinal cord tissue.

    abstract::The contribution of chondroitin sulfate proteoglycan (CSPG) in the suppression of axonal growth in rat spinal cord has been examined by means of an in vitro bioassay in which regenerating neurons are grown on tissue section substrata. Dissociated embryonic chick dorsal root ganglionic neurons were grown on normal and ...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.1998.6951

    authors: Zuo J,Neubauer D,Dyess K,Ferguson TA,Muir D

    更新日期:1998-12-01 00:00:00

  • Striatal deafferentation increases dopaminergic neurogenesis in the adult olfactory bulb.

    abstract::Dopaminergic loss is known to be one of the major hallmarks of Parkinson disease (PD). In addition to its function as a neurotransmitter, dopamine plays significant roles in developmental and adult neurogenesis. Both dopaminergic deafferentation and stimulation modulate proliferation in the subventricular zone (SVZ)/o...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2005.08.028

    authors: Winner B,Geyer M,Couillard-Despres S,Aigner R,Bogdahn U,Aigner L,Kuhn G,Winkler J

    更新日期:2006-01-01 00:00:00

  • N,N-dimethyltryptamine reduces infarct size and improves functional recovery following transient focal brain ischemia in rats.

    abstract:BACKGROUND AND PURPOSE:N,N-dimethyltryptamine (DMT) is an endogenous ligand of the Sigma 1 receptor (Sig-1R) with documented in vitro cytoprotective properties against hypoxia. Our aim was to demonstrate the in vivo neuroprotective effect of DMT following ischemia-reperfusion injury in the rat brain. METHODS:Transient...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2020.113245

    authors: Nardai S,László M,Szabó A,Alpár A,Hanics J,Zahola P,Merkely B,Frecska E,Nagy Z

    更新日期:2020-05-01 00:00:00

  • Synaptic evoked potentials from regenerating dorsal root axons within fetal spinal cord tissue transplants.

    abstract::Previously injured dorsal roots were electrically stimulated to determine if regenerating sensory axons can form physiologically active synaptic contacts with neurons within fetal spinal cord tissue transplants. Dorsal rootlets, sectioned at their spinal cord entry zone, were apposed to intraspinal transplants of feta...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.1996.0101

    authors: Houle JD,Skinner RD,Garcia-Rill E,Turner KL

    更新日期:1996-06-01 00:00:00

  • A reassessment of P2X7 receptor inhibition as a neuroprotective strategy in rat models of contusion injury.

    abstract::These experiments were completed as part of an NIH "Facilities of Research Excellence in Spinal Cord Injury" contract to support independent replication of published studies that could be considered for eventual clinical testing. Recent studies have reported that selective inhibition of the P2X7 receptor improves both...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2011.06.008

    authors: Marcillo A,Frydel B,Bramlett HM,Dietrich WD

    更新日期:2012-02-01 00:00:00

  • Coding of peripheral electrical stimulation frequency in thalamocortical pathways.

    abstract::Frequency information of the environment is an important feature for sensory perception. It has been demonstrated that cortical and thalamic neurons exhibited frequency-specific responses to peripheral stimulation. In the present study, we investigated the effects of 1-100 Hz peripheral electrical stimulations on vari...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2005.07.019

    authors: Yang H,Chang JY,Woodward DJ,Baccalá LA,Han JS,Luo F

    更新日期:2005-11-01 00:00:00

  • Neonatal ablation of the nigrostriatal dopamine pathway does not influence limb development in rats.

    abstract::Hemiparkinson-hemiatrophy syndrome (HP-HA) is associated with skeletal hemiatrophy and the later development of parkinsonism. It is generally assumed that this phenotype is due to the combination of dysfunction of the basal ganglia (e.g., substantia nigra compacta and/or other related structures), causing parkinsonism...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.2002.8005

    authors: Hebb MO,Lang AE,Fletcher PJ,Chambers JW,Lozano AM

    更新日期:2002-10-01 00:00:00

  • Axon regeneration in peripheral nerves is enhanced by proteoglycan degradation.

    abstract::Regeneration of axons in the peripheral nervous system is enhanced by the removal of glycosaminoglycan side chains (GAGs) of chondroitin sulfate proteoglycans. However, some axons regenerate poorly despite such treatment, suggesting the existence of additional inhibitors. We compared the effects of enzymatic removal o...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2005.04.007

    authors: Groves ML,McKeon R,Werner E,Nagarsheth M,Meador W,English AW

    更新日期:2005-10-01 00:00:00

  • Mutation analysis of the glial cell line-derived neurotrophic factor gene in Parkinson's disease.

    abstract::Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for nigrostriatal dopaminergic, central cholinergic, and motoneurons. GDNF also prevents the neuronal loss in experimental animal models for Parkinson's disease (PD). We have now investigated the GDNF gene for possible mutations in a group ...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.1998.6857

    authors: Wartiovaara K,Hytönen M,Vuori M,Paulin L,Rinne J,Sariola H

    更新日期:1998-08-01 00:00:00

  • Controlled release of nerve growth factor enhances sciatic nerve regeneration.

    abstract::Based on previous studies demonstrating the potential of growth factors to enhance peripheral nerve regeneration, we developed a novel growth factor delivery system to provide sustained delivery of nerve growth factor (NGF). This delivery system uses heparin to immobilize NGF and slow its diffusion from a fibrin matri...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/s0014-4886(03)00258-9

    authors: Lee AC,Yu VM,Lowe JB 3rd,Brenner MJ,Hunter DA,Mackinnon SE,Sakiyama-Elbert SE

    更新日期:2003-11-01 00:00:00

  • Osteopontin expression in activated glial cells following mechanical- or toxin-induced nigral dopaminergic cell loss.

    abstract::Osteopontin (OPN) is a glycosylated phosphoprotein that regulates both oxidative stress and inflammatory processes. OPN is present in the rat substantia nigra (SN) and both protein and mRNA levels are up-regulated following a pro-inflammatory insult produced by lipopolysaccharide. We now report on the effects of lesio...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2007.05.030

    authors: Iczkiewicz J,Rose S,Jenner P

    更新日期:2007-09-01 00:00:00

  • Effects of fasting during gestation on brain development in BALB/c mice.

    abstract::The development of the corpus callosum (CC) was examined in BALB/cCF mice in relation to the effects of a 32-h period of maternal fasting. The treatment was imposed on days 15 and 16 during gestation, which immediately precedes the time when the initial callosal axons cross the midline. The BALB/c strain was used beca...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/0014-4886(84)90176-6

    authors: Wainwright P,Gagnon M

    更新日期:1984-07-01 00:00:00

  • NMDA receptors in the inferior colliculus are critically involved in audiogenic seizures in the adult rats with neonatal hypothyroidism.

    abstract::The effects of N-methyl-d-aspartate (NMDA) and non-NMDA receptor antagonists were compared on audiogenic seizures in the rats neonatally exposed to propylthiouracil (PTU). The rats treated with 0.02% PTU through mother's milk during days 0-19 after delivery showed a high incidence of audiogenic seizures consisting of ...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.1998.6871

    authors: Higashiyama A,Ishida N,Nishimura T,Yasuda S,Kuroda Y,McEwen BS,Kato N

    更新日期:1998-09-01 00:00:00

  • The role of growth factors as a therapeutic approach to demyelinating disease.

    abstract::A variety of growth factors are being explored as therapeutic agents relevant to the axonal and oligodendroglial deficits that occur as a result of demyelinating lesions such as are evident in Multiple Sclerosis (MS). This review focuses on five such proteins that are present in the lesion site and impact oligodendroc...

    journal_title:Experimental neurology

    pub_type: 杂志文章,评审

    doi:10.1016/j.expneurol.2016.02.023

    authors: Huang Y,Dreyfus CF

    更新日期:2016-09-01 00:00:00

  • Exogenous BDNF enhances the integration of chronically injured axons that regenerate through a peripheral nerve grafted into a chondroitinase-treated spinal cord injury site.

    abstract::Although axons lose some of their intrinsic capacity for growth after their developmental period, some axons retain the potential for regrowth after injury. When provided with a growth-promoting substrate such as a peripheral nerve graft (PNG), severed axons regenerate into and through the graft; however, they stop wh...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2012.09.011

    authors: Tom VJ,Sandrow-Feinberg HR,Miller K,Domitrovich C,Bouyer J,Zhukareva V,Klaw MC,Lemay MA,Houlé JD

    更新日期:2013-01-01 00:00:00

  • Time course and age dependence of motor neuron death following facial nerve crush injury: role of fibroblast growth factor.

    abstract::Peripheral nerve crush injury (PNCI) has been used for many years in adult animals to study central and peripheral changes related to regeneration across the injury site. While these adult animals experience full recovery with no neuronal cell loss following PNCI, it has been noted that the injury in perinatal animals...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.1999.7014

    authors: Kuzis K,Coffin JD,Eckenstein FP

    更新日期:1999-05-01 00:00:00

  • The earliest patterns of neuronal differentiation and migration in the mammalian central nervous system.

    abstract::With the use of four independent cell markers and Brd-U birthdating we have charted the earliest stages of neuronal differentiation and migration in the developing rat central nervous system, including the cortex, spinal cord, and retina. One of the markers, the monoclonal antibody 2G12, labeled a large subpopulation ...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.1995.1031

    authors: Brittis PA,Meiri K,Dent E,Silver J

    更新日期:1995-07-01 00:00:00

  • Effect of gum hardness on chewing pattern.

    abstract::Chewing rhythms are set by a putative central pattern generator whose output is influenced by sensory feedback. In this study we assessed how an altered feedback imposed by changing the hardness of a gum bolus modifies the timing of chewing, the maximal gape, and the activity in the masseter muscle on the chewing side...

    journal_title:Experimental neurology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/0014-4886(86)90292-x

    authors: Plesh O,Bishop B,McCall W

    更新日期:1986-06-01 00:00:00

  • Antisense knock down of TRPA1, but not TRPM8, alleviates cold hyperalgesia after spinal nerve ligation in rats.

    abstract::Patients with neuropathic pain frequently experience hypersensitivity to cold stimulation. However, the underlying mechanisms of this enhanced sensitivity to cold are not well understood. After partial nerve injury, the transient receptor potential ion channel TRPV1 increases in the intact small dorsal root ganglion (...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2006.01.031

    authors: Katsura H,Obata K,Mizushima T,Yamanaka H,Kobayashi K,Dai Y,Fukuoka T,Tokunaga A,Sakagami M,Noguchi K

    更新日期:2006-07-01 00:00:00

  • Neurogenesis in postnatal mouse dorsal root ganglia.

    abstract::Neurogenesis continues in various regions of the central nervous system (CNS) throughout life. As the mitogen basic fibroblast growth factor (bFGF) can proliferate neuronal precursors of CNS neurons in culture, and is also upregulated within adult dorsal root ganglia following axotomy, it is possible that the postnata...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1006/exnr.2001.7761

    authors: Namaka MP,Sawchuk M,MacDonald SC,Jordan LM,Hochman S

    更新日期:2001-11-01 00:00:00

  • One hour electrical stimulation accelerates functional recovery after femoral nerve repair.

    abstract::The clinical outcome of peripheral nerve injuries requiring surgical repair is usually poor and efficient therapies do not exist. Recent work has suggested that low-frequency electrical stimulation of the severed nerve which produces repeated discharges of the parent motoneuron perikarya positively influences axonal r...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2007.08.005

    authors: Ahlborn P,Schachner M,Irintchev A

    更新日期:2007-11-01 00:00:00

  • Pharmacological hypothermia induced neurovascular protection after severe stroke of transient middle cerebral artery occlusion in mice.

    abstract::Therapeutic hypothermia is a potential protective strategy after stroke. The present study evaluated the neurovascular protective potential of pharmacological hypothermia induced by the neurotensin receptor 1 agonist HPI-201 after severe ischemic stroke. Adult C57BL/6 mice were subjected to filament insertion-induced ...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/j.expneurol.2019.113133

    authors: Zhao Y,Wei ZZ,Lee JH,Gu X,Sun J,Dix TA,Wei L,Yu SP

    更新日期:2020-03-01 00:00:00

  • Effects of treatment with scopolamine and naloxone, singly and in combination, on amygdala kindling.

    abstract::Scopolamine and naloxone were administered singly and in combination to different groups of rats undergoing electrical kindling of the amygdala. Scopolamine significantly reduced the maximal seizure stage attained during 15 drug sessions and increased the total number of afterdischarges required to kindle a generalize...

    journal_title:Experimental neurology

    pub_type: 杂志文章

    doi:10.1016/0014-4886(87)90171-3

    authors: Cain DP,McKitrick DJ,Desborough KA

    更新日期:1987-04-01 00:00:00