Abstract:
:The present study evaluates the temporal relationships between increases in glial fibrillary acidic protein (GFAP) mRNA, GFA protein levels, and GFAP immunostaining in the hippocampus of adult rats following unilateral lesions of the entorhinal cortex (EC). GFAP mRNA levels were assessed at 12 h, 1, 2, 4, 6, 8, 10, 12, 14, and 30 days postlesion by dot blot assays using 35S-labeled cRNA probes against the mRNA. Animals were also prepared for in situ hybridization during the peak of GFAP mRNA expression (2 days postlesion) to explore the nature of individual differences in the spatial extent of the increases. GFA protein levels were assessed by Western blot and dot immunoblot techniques in a separate group of animals prepared at 1, 2, 4, 6, 8, and 10 days postlesion and by immunostaining at 1, 2, 4, 6, and 8 days postlesion. The dot blot analyses of GFAP mRNA levels confirmed previous studies, in that we observed dramatic increases in the levels of GFAP mRNA in the hippocampus ipsilateral to the EC lesions. The increases were biphasic, with a large peak in mRNA levels at 1-2 days postlesion (about 10-fold greater than control) and a second peak at 6-8 days. In most animals, the increases were predominantly ipsilateral to the lesion. However, in some animals, there were also large increases on the contralateral side. In situ hybridization experiments revealed two different spatial patterns of increased gene expression, one in which the increases in GFAP mRNA occurred bilaterally and one in which increases were restricted primarily to the hippocampus ipsilateral to the lesion. Immunochemical measures revealed that GFA protein levels increased gradually in the hippocampus ipsilateral to the lesion, reaching a peak at about 2-fold higher than control at 4 days postlesion, and then remained near this level until at least 10 days postlesion. In the contralateral hippocampus, GFA protein levels were increased to about the same extent as on the ipsilateral side at 1, 2, and 4 days postlesion, but then began to decline, returning to near control levels by 8 days. Increases in immunostaining occurred with about the same time course as the increases in GFA protein levels as measured immunochemically. These results define the temporal relationship between increases in GFAP mRNA and increases in GFA protein, providing new insights into the regulation of gene expression in reactive astrocytes.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Steward O,Kelley MS,Torre ERdoi
10.1006/exnr.1993.1187subject
Has Abstractpub_date
1993-12-01 00:00:00pages
167-83issue
2eissn
0014-4886issn
1090-2430pii
S0014-4886(83)71187-8journal_volume
124pub_type
杂志文章abstract::In this study, we examined modulations in phosphatase and tensin homolog (PTEN) and mammalian target of rapamycin (mTOR) protein expression after a lateral C2 hemisection and subsequent intermittent hypoxia (IH) exposure and training, which initiates respiratory motor plasticity and recovery. PTEN and mTOR are signifi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2013.05.013
更新日期:2013-10-01 00:00:00
abstract::Volume-regulated anion channels (VRACs) are critically involved in regulating cell volume, and leucine-rich repeat-containing protein 8A (LRRC8A, SWELL1) is an obligatory subunit of VRACs. Cell swelling occurs early after brain ischemia, but it is unclear whether neuronal LRRC8a contributes to ischemia-induced glutama...
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1996.6359
更新日期:1997-01-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2017.10.013
更新日期:2018-01-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:2009-05-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(91)90162-6
更新日期:1991-12-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(83)90414-4
更新日期:1983-11-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(92)90217-e
更新日期:1992-01-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2001.7734
更新日期:2001-09-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2001.7721
更新日期:2001-08-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(88)90005-2
更新日期:1988-08-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(92)90238-l
更新日期:1992-01-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.01.021
更新日期:2005-07-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2002.8021
更新日期:2002-11-01 00:00:00
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2008.10.010
更新日期:2009-01-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.12.020
更新日期:2007-04-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(86)90097-x
更新日期:1986-05-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2020.113524
更新日期:2021-02-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(87)90083-5
更新日期:1987-10-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(86)90031-2
更新日期:1986-01-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:1984-01-01 00:00:00