Abstract：
:The success of peripheral nerve regeneration depends on intrinsic properties of neurons and a favorable environment, although the mechanisms underlying the molecular events during degeneration and regeneration are still not elucidated. Schwann cells are considered one of the best candidates to be closely involved in the success of peripheral nerve regeneration. These cells and invading macrophages are responsible for clearing myelin and axon debris, creating an appropriate route for a successful regeneration. After injury, Schwann cells express galectin-3, and this has been correlated with phagocytosis; also, in the presence of galectin-3, there is inhibition of Schwann-cell proliferation in vitro. In the present study we explored, in vivo, the effects of the absence of galectin-3 on Wallerian degeneration and nerve-fiber regeneration. We crushed the sciatic nerves of galectin-3 knockout and wild-type mice, and followed the pattern of degeneration and regeneration from 24 h up to 3 weeks. We analyzed the number of myelinated fibers, axon area, fiber area, myelin area, G-ratio and immunofluorescence for beta-catenin, macrophages and Schwann cells in DAPI counterstained sections. Galectin-3 knockout mice showed earlier functional recovery and faster regeneration than the wild-type animals. We concluded that the absence of galectin-3 allowed faster regeneration, which may be associated with increased growth of Schwann cells and expression of beta-catenin. This would favor neuron survival, followed by faster myelination, culminating in a better morphological and functional outcome.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Narciso MS,Mietto Bde S,Marques SA,Soares CP,Mermelstein Cdos S,El-Cheikh MC,Martinez AMdoi
10.1016/j.expneurol.2009.01.008subject
Has Abstractpub_date
2009-05-01 00:00:00pages
7-15issue
1eissn
0014-4886issn
1090-2430pii
S0014-4886(09)00012-0journal_volume
217pub_type
杂志文章abstract：:Olfactory ensheathing cells (OEC) constitute a specialized population of glia that accompany primary olfactory axons and have been reported to facilitate axonal regeneration after spinal cord injury in vivo. In the present report we describe OEC neurotrophic factor expression and neurotrophic properties of OECs in vit...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/s0014-4886(02)00058-4
更新日期：2003-04-01 00:00:00
abstract：:Fish optic nerve sections were recently shown to be nonpermissive to growth of adult retinal axons. In addition, fish optic nerve myelin was found to inhibit growth of adult retinal axons and this inhibition was neutralized by IN-1 antibodies (known to block rat myelin-associated inhibitors). In this study we examined...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1994.1221
更新日期：1994-12-01 00:00:00
abstract：:Downregulation in the astroglial glutamate transporter EAAT2 in amyotrophic lateral sclerosis (ALS) patients and mutant SOD1 mouse models of ALS is believed to contribute to the death of motor neurons by excitotoxicity. We previously reported that caspase-3 cleaves EAAT2 at a unique cleavage consensus site located in ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2017.03.014
更新日期：2017-06-01 00:00:00
abstract：:The members of the tenascin family are involved in a number of developmental processes, mainly by their ability to regulate cell adhesion. We have here studied the distribution of mRNAs for tenascin-X, -C, and -R and the closely related molecule tenascin/J1 in the olfactory system and spinal cord. The olfactory bulb a...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2000.7543
更新日期：2000-12-01 00:00:00
abstract：:Activity-dependent alteration in synaptic efficacy is referred to as synaptic plasticity and is the believed hallmark of any learning process. Here we employed a recently validated complex maze for spatial training and investigated the impact of repeated and extensive training on basal synaptic transmission of the hip...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2006.08.025
更新日期：2007-02-01 00:00:00
abstract：:This study describes the short-term effects of intracaudate microinjections of carbachol in temporarily isolated and restrained Java monkeys. The monkeys were found to display a series of motor disturbances including blepharoptosis, facial twitches, tongue protrusions, ear flattening, torticollis, and compulsive alter...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(85)90133-5
更新日期：1985-01-01 00:00:00
abstract：:Hydrocephalic mutant mice and matched siblings at different ages were used to measure the pressure and the resistance to drainage of the cerebrospinal fluid (CSF) from the lateral ventricles and from the cisterna magna with glass micropipets. The resting CSF pressure in normal mice increased between 1 to 2 and 4 to 8 ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(85)90049-4
更新日期：1985-10-01 00:00:00
abstract：:Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system, in which the myelin sheath has been considered to be the primary target for many years. However, an increasing number of reports have focused on neurodegenerative aspects of the disease pathogenesis. Recent studies i...
journal_title：Experimental neurology
pub_type： 杂志文章,评审
doi：10.1016/j.expneurol.2009.11.019
更新日期：2010-09-01 00:00:00
abstract：:Abnormal oscillatory activity in the basal ganglia is increasingly implicated in the pathophysiology of Parkinson's disease. Such activity is recorded in patients in the form of oscillations in the local field potential (LFP) picked up in the subthalamic nucleus. Previous studies have focused on correlations between f...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2012.04.024
更新日期：2012-08-01 00:00:00
abstract：:In 3-, 9-, 24-, and 30-month-old male rats (Fischer 344), the nuclear perimeter and the density and diameter of nuclear pore complexes in the granule cells of the dentate fascia were studied. Whereas the nuclear perimeter and the diameter of nuclear pore complexes did not change as a function of age, there was a signi...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(87)90314-1
更新日期：1987-03-01 00:00:00
abstract：:Youth is a strong predictor of functional recovery after peripheral nerve repair, while adulthood is commonly associated with poor outcome. Identification of the factors responsible for this difference could form the basis for strategies to improve regeneration in adults. Preferential reinnervation of motor pathways b...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2000.7538
更新日期：2001-01-01 00:00:00
abstract：:We have investigated the long-term effects of 15-16 min or 19-20 min of perinatal asphyxia on D1, D2, and D3 receptors (analyzed by quantitative autoradiography) in the mesotelencephalic dopamine systems of the 4-week-old rat. Perinatal asphyxia reduced D1 antagonist binding ([3H]SCH 23390 in the presence of ketanseri...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1997.6497
更新日期：1997-07-01 00:00:00
abstract：:Pompe disease is an inherited disorder due to a mutation in the gene that encodes acid α-glucosidase (GAA). Children with infantile-onset Pompe disease develop progressive hypotonic weakness and cardiopulmonary insufficiency that may eventually require mechanical ventilation (MV). Our team conducted a first in human t...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2016.07.013
更新日期：2017-01-01 00:00:00
abstract：:The strength of contractures, produced by 15 to 146 mM [K]0 (as L-glutamate), was measured in isolated small bundles of muscle fibers from the fast-twitch extensor digitorum longus and from the slow-twitch soleus of normal and dystrophic (C57 BL/6J dy2J/dy2J) mice. The analysis of the relation between the maximal ampl...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(85)90179-7
更新日期：1985-03-01 00:00:00
abstract：:Fluoxetine is one of the most promising drugs for improving clinical outcome in patients with ischemic stroke. This in vivo study investigated the hypothesis that fluoxetine may affect HIF-1α-Netrin/VEGF cascade, angiogenesis and neuroprotection using a rat model of transient middle cerebral artery occlusion (tMCAO). ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2020.113312
更新日期：2020-07-01 00:00:00
abstract：:Mitochondria actively participate in neurotransmission by providing energy (ATP) and maintaining normative concentrations of reactive oxygen species (ROS) in both presynaptic and postsynaptic elements. In human and animal epilepsies, ATP-producing respiratory rates driven by mitochondrial respiratory complex (MRC) I a...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2013.11.005
更新日期：2014-01-01 00:00:00
abstract：:Triggering receptor expressed on myeloid cells-2 (TREM2) was a newly identified receptor expressed on microglia. Several observations support the hypothesis that TREM2 variation may confer susceptibility to Parkinson's disease (PD). Therefore, in this paper, we explored the role of TREM2 in the 1-methyl-4-phenyl-1,2,3...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2018.01.016
更新日期：2018-04-01 00:00:00
abstract：:Fibroblast Growth Factors (FGFs) and their receptors (FGFRs) are widely expressed in the mature nervous system and are thought to mediate plasticity and repair. We report the generation of transgenic mice that can be induced to express a dominant-negative FGFR (dnFGFR) in select neuronal populations. We show that a mo...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2005.12.020
更新日期：2006-04-01 00:00:00
abstract：:Dopamine (DA) affects GABA neuronal function in the striatum and together these neurotransmitters play a large role in locomotor function. We recently reported that unilateral striatal administration of GDNF, a growth factor that has neurotrophic effects on DA neurons and enhances DA release, bilaterally increased str...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2009.05.013
更新日期：2009-09-01 00:00:00
abstract：:Damage to the fimbria-fornix, and separately to the perforant path, leads to distinct and dramatic time-dependent increases in glial fibrillary acidic protein immunoreactivity (GFAP-IR) in specific areas of the hippocampal formation. Specifically, fimbria-fornix lesions resulted in an increase in the GFAP-IR in the py...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(88)90073-8
更新日期：1988-10-01 00:00:00
abstract：:Zebrafish is an excellent model to study central nervous system (CNS) axonal degeneration and regeneration since we can observe these processes in vivo and in real time in transparent larvae. Previous studies have shown that Mauthner cell (M-cell) axon regenerates poorly after mechanical spinal cord injury. Inconsiste...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2017.10.028
更新日期：2018-02-01 00:00:00
abstract：:Infantile spasms are generalized convulsive seizures seen in the first year of life. They respond poorly to conventional anticonvulsants, but are often controlled by adrenocorticotropic hormone (ACTH) therapy. Other childhood seizures are also responsive to ACTH. The present study tested the effects of ACTH and relate...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2002.7874
更新日期：2002-05-01 00:00:00
abstract：:Neonatal hypoxia-ischemia (HI) is the most common cause of brain injury in neonates, which leads to high neonatal mortality and severe neurological morbidity in later life (Vannucci, 2000; Volpe, 2001). Yet the molecular mechanisms of neuronal death and brain damage induced by neonatal HI remain largely elusive. Herei...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2018.02.016
更新日期：2018-06-01 00:00:00
abstract：:The effects of superior cervical ganglion stimulation and the local application of norepinephrine or its antagonist propranolol were studied in 18 pineal cells. In 61% of the pineal cells, stimulation elicited excitation; the same cells also responded by excitation to norepinephrine ejection. When stimulation and nore...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(85)90149-9
更新日期：1985-12-01 00:00:00
abstract：:Pretreatment with the synthetic glucocorticoid dexamethasone prevents hypoxic-ischemic brain damage in 7-day-old neonatal rats. We presently characterize the response further by examining the effect of varying the age, the glucocorticoid, and the time of injection and by examining whether fasting can influence the res...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/0014-4886(95)90065-9
更新日期：1995-03-01 00:00:00
abstract：:Mismatches between tissue perfusion-weighted imaging (PWI; an index of blood flow deficit) and cellular diffusion-weighted imaging (DWI; an index of tissue injury) provide information on potentially salvageable penumbra tissue in focal stroke and can identify "treatable" stroke patients. The present pre-clinical studi...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2008.03.011
更新日期：2008-07-01 00:00:00
abstract：:Multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE), an animal model of MS, are inflammatory demyelinating diseases of the central nervous system. The inflammatory attacks lead to glial dysfunction and death, axonal damage, and neurological deficits. Numerous studies in rat suggest that extrace...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2004.05.023
更新日期：2004-09-01 00:00:00
abstract：:As in Alzheimer's disease, brains of Guam Chamorros with amyotrophic lateral sclerosis (ALS) and Parkinsonism-dementia complex (PDC) contain intraneuronal-paired helical filaments composed of accumulated phosphorylated tau protein. Tau mRNA expression in rat neuronal cultures-normally modulated by glutamate-increases ...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.1998.6962
更新日期：1999-01-01 00:00:00
abstract：:Transforming growth factor-alpha (TGF-alpha), previously identified as a major member of the epidermal growth factor (EGF) family of growth factors, plays a role in proliferation, differentiation, and survival of neuronal and glial precursors and is implicated in development of the nervous system. However, its roles i...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1006/exnr.2001.7771
更新日期：2001-11-01 00:00:00
abstract：:Accumulating evidence shows alterations in the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) in ALS patients and in animal models of disease, mainly by endothelial cell (EC) damage. Repair of the altered barrier in the CNS by replacement of ECs via cell transplantation may be a new therapeutic approac...
journal_title：Experimental neurology
pub_type： 杂志文章
doi：10.1016/j.expneurol.2018.08.012
更新日期：2018-12-01 00:00:00
