Abstract：
:In this study we examined the presence of dopamine D1 receptors in monkey amniotic epithelial cells (MAEC) using RT-PCR and radioligand binding experiments. We found that MAEC express D1 receptor mRNA that is having 99% homology with human dopamine D1 receptors. Saturation binding studies using [3H]SCH-23390 showed a high affinity D1 site with K(D) and Bmax values of 0.82 +/- 0.12 nM and 20.77 +/- 4.22 fmol/mg protein, respectively. Competition experiments showed that selective D1, but not D2, antagonists are potent displacers of [3H]SCH 23390 binding with a rank order of potency that is consistent with the pharmacology of the dopaminergic D1 site. These data provide, for the first time, compelling evidence that MAEC natively express D1 mRNA and binding sites and suggest that it may be a potential primate cell model to study D1 receptors and to explore new selective drugs active at these receptors.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Elwan MA,Ishii T,Ono F,Sakuragawa Ndoi
10.1016/s0304-3940(99)00016-6keywords:
subject
Has Abstractpub_date
1999-02-26 00:00:00pages
9-12issue
1eissn
0304-3940issn
1872-7972pii
S0304-3940(99)00016-6journal_volume
262pub_type
杂志文章abstract：:The breakdown in brain ionic homeostasis during energy deprivation (anoxic depolarization [AD]) is intimately linked to neuronal injury. We studied the role of one particular route of Na+ influx, voltage-sensitive Na+ channels, in the AD induced by O2 and/or glucose deprivation. We recorded extracellular Na+ concentra...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00728-4
更新日期：1999-11-05 00:00:00
abstract：:Abnormal neural activity generated at a site of nerve injury is thought to contribute to the development of dysaesthesia. Vanilloid receptor 1 (TRPV1), a transducer of noxious stimuli, may be involved in the initiation of this abnormal activity and could provide a useful therapeutic target. We investigated the effect ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.06.088
更新日期：2008-09-26 00:00:00
abstract：:Prolyl endopeptidase (PEP; EC 3.4.21.26) cleaves the Pro-Arg bond of arginine-vasopressin (AVP). This study investigated the effects of PEP inhibitors, 1-[1-(benzyloxycarbonyl)-L-prolyl]prolinal (Z-Pro-prolinal) and 1-[3-(2-indanylacetyl)-L-thioprolyl]pyrrolidine (Z-321), on the AVP content in the septum of rats. Oral...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11821-d
更新日期：1995-08-18 00:00:00
abstract：:To investigate potential trophic actions of extracellular ATP in human astrocytes, we have examined mitogenic signaling by purinergic receptors in cultures prepared from first trimester rostral central nervous system tissue. We found that ATP and ATPgammaS, a hydrolysis-resistant analog, stimulated DNA synthesis, ther...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(98)00067-6
更新日期：1998-02-20 00:00:00
abstract：:Acute stress can provoke apoptosis of the retina cells, and up-regulation of calcitonin gene related peptide (CGRP) in retina. However the role of CGRP in the pathology of the stress induced apoptosis of the retina cells is still elusive. The aim of this study was to investigate the endogenous of CGRP on retinal cell ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2011.06.049
更新日期：2011-08-26 00:00:00
abstract：:The standard pseudohomophone effect in the lexical decision task, i.e. longer response times and higher error rates for pseudohomophones compared with spelling controls, is commonly explained by an orthography-phonology-conflict. This study tested this conflict account, using a multi-method approach including particip...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.03.010
更新日期：2009-05-15 00:00:00
abstract：:Recent findings document numerous interactions between neuronal and glial systems that likely play a role in the pathophysiology of depression. These findings suggest that glia-derived neurotrophic protein S100B may play a significant role in developing depression. To test the relationship between S100B and depressive...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.09.003
更新日期：2012-11-07 00:00:00
abstract：:The forebrain is one of the important suprapontine micturition centres. Previous studies have shown that electrical stimulation of the frontal lobe and the anterior cingulate gyrus elicited either inhibition or facilitation of bladder contraction. Patients with frontal lobe tumours and aneurysms showed micturition dis...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.02.015
更新日期：2010-03-31 00:00:00
abstract：:Glycinergic inhibitory neurotransmission plays a pivotal role in the development of neuropathic pain. The glycine concentration in the synaptic cleft is controlled by the glycine transporters GlyT1 and GlyT2. GlyT1 is expressed throughout the central nervous system, while GlyT2 is exclusively located in glycinergic ne...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2015.06.026
更新日期：2015-07-23 00:00:00
abstract：:We and others have previously reported that neurons expressing neuronal nitric oxide synthase (nNOS) do not co-express GTP cyclohydrolase I, the enzyme that synthesize its cofactor tetrahydrobiopterin (BH4). BH4 is released from catecholaminergic cells and nNOS-expressing cells are located close to BH4-producing catec...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2003.08.032
更新日期：2003-12-04 00:00:00
abstract：:The perinatal period of brain is characterized by dynamic changes in structure and high propensity for epilepsy. Animal models have shown that alterations of AMPA receptor (AMPAR) assembly or function may be related to seizure-induced cell damage, long-lasting impairments in brain development and seizure threshold. Ho...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.08.006
更新日期：2008-10-17 00:00:00
abstract：:Noradrenaline (NA)- and neuropeptide Y (NPY)-containing cell bodies were found to occur in high numbers (>75% of all cells were positive) in the human superior cervical ganglion and distributed homogeneously throughout the ganglion and showed colocalisation. A few cell bodies were VIP-immunoreactive (-ir) (less than 5...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00115-9
更新日期：1999-03-26 00:00:00
abstract：:Calpain activity of nerve growth factor (NGF)-induced rat pheochromocytoma (PC12) cells shows a transient diminution in the early phase of differentiation. Calpain activity can be further decreased by a cell-permeable calpain inhibitor, calpeptin, which enhances the effect of NGF by stimulating neurite elongation. The...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90246-1
更新日期：1994-03-28 00:00:00
abstract：:beta-Bungarotoxin (BuTx)-binding cells were immunocytochemically examined in the developing rat cerebellum. The tissue was incubated with BuTx and then immunostained with antiserum against its toxoid. On postnatal day 6, only Golgi cells were positive for immunoreaction. Immunoreactive Golgi cells were reduced in numb...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(87)90187-x
更新日期：1987-04-23 00:00:00
abstract：:The activities of monoamine oxidase (MAO)-A and -B in subcellular fractions of bovine retina were determined using serotonin and beta-phenylethylamine, respectively, as substrates. The subcellular fractions were a P1 fraction containing photoreceptor cell synaptosomes and a P2 fraction enriched with small synaptosomes...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(81)90382-7
更新日期：1981-01-20 00:00:00
abstract：:The effect of intracerebroventricular (i.c.v.) injection of brain natriuretic peptide (BNP), a novel peptide purified from the porcine brain, on arginine vasopressin (AVP) secretion was studied in conscious, unrestrained rats and was compared with that of atrial natriuretic polypeptide (ANP). I.c.v. administration of ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(88)90661-1
更新日期：1988-12-19 00:00:00
abstract：:Localized moderate cooling (+10 degrees C) of the anterior and ventral part of the raphe dorsalis induced cortical synchronization and ponto-geniculo-occipital (PGO) waves followed sometimes by paradoxical sleep. Cooling the region of the raphe magnus produced cortical arousal. After depletion of brain 5-hydroxytrypta...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(76)90077-x
更新日期：1976-11-01 00:00:00
abstract：:The objective of the present study was to determine differences in neural networks between expert and novice archers during an archery pre-performance routine period (PPR). The experiment was conducted with eight world-class competitor or Olympic medalist archers and eight novices. In the experiment, the task was to s...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.09.018
更新日期：2008-11-21 00:00:00
abstract：:Alpha-2 macroglobulin (A2M) is a serum pan-protease inhibitor that is related with the pathogenesis of Alzheimer's disease (AD) through its ability to mediate amyloid beta degradation. Recently, it has been reported that the I1000V polymorphism in A2M gene might increase the risk of AD. In the present study, we invest...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)01330-6
更新日期：2000-08-25 00:00:00
abstract：:The light microscopic localization of gamma-aminobutyric acidB (GABAB) receptors in the rat brain has been accomplished using in vitro autoradiography with [3H]baclofen as a ligand. Initial biochemical studies indicated this compound specifically bound to slide-mounted tissue sections with high affinity in a saturable...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(85)90126-0
更新日期：1985-05-14 00:00:00
abstract：:The neuromuscular junction (NMJ) is the vehicle for fast, reliable and robust communication between motor neuron and muscle. The unparalleled accessibility of this synapse to morphological, electrophysiological and genetic analysis has yielded an in depth understanding of many molecular components mediating its format...
journal_title：Neuroscience letters
pub_type： 杂志文章,评审
doi：10.1016/j.neulet.2020.135014
更新日期：2020-07-13 00:00:00
abstract：:Deficit in balance control is a common and often an initial disabling symptom of multiple sclerosis (MS). The aim of the study was to investigate the organization of anticipatory and compensatory postural adjustments in individuals with MS dealing with external perturbations. Ten individuals with MS and ten age-and-ge...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2015.02.050
更新日期：2015-03-30 00:00:00
abstract：:Mutations in DJ-1 cause familial Parkinson's disease (PD). The expression pattern of DJ-1 in the brain remains controversial. In the present study, we used DJ-1 deficient mice as negative controls and examined DJ-1 mRNA expression in mouse brains. In sequential double labeling on the same sections, in situ hybridizati...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.09.024
更新日期：2009-11-20 00:00:00
abstract：:Consummatory successive negative contrast (cSNC) occurs when animals exposed to an unexpected downshift from a high palatable reward (e.g., 32% sucrose solution) to a less preferred one (e.g., 4% sucrose solution) show an abrupt and transient suppression of the consummatory response, compared with control animals that...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2018.07.013
更新日期：2018-09-25 00:00:00
abstract：:The cognitive deficits observed in schizophrenia are considered a core feature of the disease. Neuregulin-1 is a risk gene for schizophrenia that is involved in many neurodevelopmental and synaptic plasticity-related processes relevant to schizophrenia. Here, we have utilized a rat model (Nrg1(Tn)), which is hypomorph...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.10.018
更新日期：2012-12-07 00:00:00
abstract：:Sphingosine-1-phosphate (S1P) has been demonstrated to be an important regulator of cell death and survival. Although it has been suggested that the sphingolipid may act as a neuroprotector in the cell apoptosis induced by traumatic brain injury, the mechanisms involved in this action are unknown. In this study, the r...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.12.070
更新日期：2010-02-12 00:00:00
abstract：:We present a comprehensive analysis of the change in event-related potential (ERP) due to task difficulty during a visual oddball task. Specifically, we investigated the inter-subject difference in difficulty-related change of ERP patterns using single-trial ERP analysis focusing on P300 and P2 components. ERPs were r...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.09.004
更新日期：2008-11-14 00:00:00
abstract：:Evidence indicates that adaptive responses to systemic stress occur in retina, as indicated by the changes in sympathetic and sensory afferent neurotransmitters in the retina when under stressful stimulation, such as acute myocardial infarction. The aim of this study was to investigate the potential association of the...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.10.006
更新日期：2011-01-03 00:00:00
abstract：:We describe a novel and powerful extracellular method for the staining of a single neuron identified by electrophysiological criteria. This single-unit technique involves the use of glass micro-electrodes (tip diameter: 1.5-2.5 microns) filled with a saline solution (NaCl; 0.5 M) containing 1.5% of biocytin or Neurobi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90332-8
更新日期：1994-04-11 00:00:00
abstract：:Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal culture...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.04.089
更新日期：2004-08-05 00:00:00
